• Bulletin of the Korean Chemical Society
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• v.23 no.8
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• pp.1073-1077
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• 2002

Binding of dodecyl trimethylammonium bromide (DTAB) to human and bovine hemoglobin and globin samples has been investigated in 50 mM glycine buffer pH = 10, I = 0.0318 and 300 K by equilibrium dialysis and temperature scanning spectrophotometry techniques and method for calculation of average hydrophobicity. The binding data has been analyzed, in terms of binding capacity concept $({\theta})$, Hill coefficient (nH) and intrinsic Gibbs free energy of binding $({\Delta}Gbv).$ The results of binding data, melting point (Tm) and average hydrophobicity show that human hemoglobin has more structural stability than bovine hemoglobin sample. Moreover the results of binding data analysis represent the systems with two and one sets of binding sites for hemoglobin and globin, respectively. It seems that the destabilization of hemoglobin structure due to removal of heme group, is responsible of such behavior. The results indicating the removal of heme group from hemoglobin caused the depletion of first binding set as an electrostatic site upon interaction with DTAB and exposing the hydrophobic patches for protein.

• Journal of electromagnetic engineering and science
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• v.19 no.1
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• pp.6-12
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• 2019

An IE-FFT algorithm is implemented and applied to the electromagnetic (EM) solution of perfect electric conducting (PEC) scattering problems. The solution of the method of moments (MoM), based on the magnetic field integral equation (MFIE), is obtained for PEC objects with closed surfaces. The IE-FFT algorithm uses a uniform Cartesian grid to apply a global fast Fourier transform (FFT), which leads to significantly reduce memory requirement and speed up CPU with an iterative solver. The IE-FFT algorithm utilizes two discretizations, one for the unknown induced surface current on the planar triangular patches of 3D arbitrary geometries and the other on a uniform Cartesian grid for interpolating the free-space Green's function. The uniform interpolation of the Green's functions allows for a global FFT for far-field interaction terms, and the near-field interaction terms should be adequately corrected. A 3D block-Toeplitz structure for the Lagrangian interpolation of the Green's function is proposed. The MFIE formulation with the IE-FFT algorithm, without the help of a preconditioner, is converged in certain iterations with a generalized minimal residual (GMRES) method. The complexity of the IE-FFT is found to be approximately $O(N^{1.5})$and $O(N^{1.5}logN)$ for memory requirements and CPU time, respectively.

• BMB Reports
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• v.37 no.2
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• pp.153-158
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• 2004

The acid-labile subunit (ALS) is known to interact with the IGF binding protein (IGFBP) in the presence of insulin-like growth factors (IGFs). Studies, however, indicate that ALS forms a doublet with IGFBP3, independent of IGFs. To characterize the structural domain required for the IGF-free ALS-IGFBP3 interaction, seven recombinant human IGFBP3 mutants were generated: three deletion mutants and four site-specific mutants that had altering N-terminal regions of IGFBP3. ALS and IGFBP3 mRNAs were co-injected into Xenopus oocytes, and their products were cross-linked and immunoprecipitated using antisera against ALS or IGFBP3. Among the deletion mutants, the mutant of D40 (deleted in 11-40th amino acids) exerted no effect in the interaction with ALS, while D60 (${\Delta}11$-60) demonstrated a moderate reduction. D88 (${\Delta}11$-88), however, showed a significant decrease. In the case of site-specific mutants, the mutation that alterated the IGF binding site (codons 56 or 80) exerted a significant reduction in the interaction, whereas codons 72 or 87 showed no significant change in the interaction with ALS. The stability of the ALS-IGFBP3 interaction was analyzed according to a time-dependent mode. Consistent with the binding study, mutants on the IGF binding sites (56 or 80) consistently show a weakness in the ALS-IGFBP3 interaction when compared to the mutants that covered the non-IGF binding sites (72 or 87). This study suggests that the N-terminal of IGFBP3, especially the IGF binding site, plays an important role in interacting with ALS as well as in stabilizing the dual complex, independent of IGFs.

• Tribology and Lubricants
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• v.8 no.1
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• pp.63-69
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• 1992

In order to gain better understanding of wear behaviors of TiN-coated boron cast iron, tests and analyses were conducted with block-on disc type tribometer. TiN layer of thickness $2 \mu m$ and $4 \mu m$, coated by cathodic arc evaporation process, were experimentally investigated with the variation of applied load and sliding speed under dry sliding condition. Wear characteristics were expressed in terms of the three-dimentional wear map as well as the wear rate vs sliding speed and load. Comparisons of wear and friction characteristics between coated cast irons and uncoated cast irns were also made. Wear mechanism of TiN layer was explained in view of surface interaction between the mating surfaces. The thicker coating exhibited higher hardness and adhesion strength. the significance of stresses at the surface and in the subsurface was briefly discussed in relation to the wear behavior.

• Journal of the Semiconductor & Display Technology
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• v.9 no.3
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• pp.1-4
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• 2010

First-principles calculations based on spin density functional theory are performed to study the spin-resolved electronic properties of AlN doped with a Cu concentration of 6.25%-18.75%. The ferromagnetic state is more energetically favorable state than the antiferromagnetic state or the nonmagnetic state. For $Al_{0.9375}Cu_{0.0625}N$, a global magnetic moment of 1.26 mB per supercell, with a localized magnetic moment of 0.75 $m_B$ per Cu atom is found. The magnetic moment is reduced due to an increase in the number of Cu atoms occupying adjacent cation lattice position. For $Al_{0.8125}Cu_{0.1875}N$, the magnetism of the supercell disappears by the interaction of the neighboring Cu atoms. The nonmagnetic to ferromagnetic phase transition is found to occur at this Cu concentration. The range of concentrations that are spin-polarized should be restricted within very narrow.

• Bulletin of the Korean Chemical Society
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• v.31 no.11
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• pp.3099-3102
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• 2010

A label-free DNA detection method was developed for a simple electrochemical DNA sensor with a short assay time. Self-assembled monolayers of peptide nucleic acid were used as a probe on gold electrodes. The formation of the self-assembled monolayers on the gold electrodes was successfully checked by means of cyclic voltammetry. The target DNA, hybridized with peptide nucleic acid, can be detected by the anodic peak current of ferrocene dendrimers, which interact electrostatically with the target DNA. This anodic peak current was measured by square wave voltammetry at 0.3 V to decrease the detection limit on the order of the nanomolar concentrations. As a result, the label-free electrochemical DNA sensor can detect the target DNA in concentrations ranging from 1 nM to $1\;{\mu}M$ with a detection limit of 1 nM.

• Mass Spectrometry Letters
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• v.12 no.4
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• pp.179-185
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• 2021

Collision-induced dissociation (CID) combined with electrospray ionization mass spectrometry (ESI-MS) was used to obtain structural information on rat islet amyloid polypeptide (rIAPP) monomers (M) and dimers (D) observed in the multiply charged state in the MS spectrum. MS/MS analysis indicated that the rIAPP monomers adopt distinct structures depending on the molecular ion charge state. Peptide bond dissociation between L₂₇ and P₂₈ was observed in the MS/MS spectra of rIAPP monomers, regardless of the monomer molecular ion charge state. MS/MS analysis of the dimers indicated that D⁵⁺ comprised M²⁺ and M³⁺ subunits, and that the peptide bond dissociation process between the L₂₇ and P₂₈ residues of the monomer subunit was also maintained. The observation of (M+ b₂₇)⁴⁺ and (M+ y₁₀)³⁺ fragment ions were deduced to originate from the two different D⁵⁺ complex geometries, the N-terminal and C-terminal interaction geometries, respectively. The fragmentation pattern of the [M_rIAPP + M_hIAPP]⁵⁺ MS/MS spectrum showed that the interaction occurred between the two N-terminal regions of M_rIAPP and M_hIAPP in the heterogeneous dimer (hetero-dimer) D⁵⁺ structure.

• Transactions of the Korean Society of Mechanical Engineers
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• v.14 no.2
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• pp.453-462
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• 1990

The interaction of forced convection-conduction with thermal radiation in laminar boundary layer over a plate fin is studied numerically. The analysis is based on complete solution whereby the heat conduction equation for the fin is solved simultaneously with the conservation equations for mass, momentum and energy in the fluid boundary layer adjacent to the fin. The fluid is a gray medium and diffusion(Rosseland) approximation is used to describe the radiative heat flux in the energy equation. The resulting boundary value problem are convection-conduction parameter N$_{c}$ and radiation-conduction parameter m, Prandtl number Pr. Numerical results are presented for gases with the Prandtl numbers of 0.7 & 5 with values of N$_{c}$ and M ranging from 0 to 10 respectively. The object of this study is to provide the first results on forced convection-radiation interaction in boundary layer flow over a semi-infinite flay plate which can be used for comparisons with future studies that will consider a more accurate expression for the radiative heat flux. The agreement of the results from the complete solution presented by E. M. Sparrow and those from this paper for the special case of M=0 is good. The overall rate of heat transfer from the fin considering radiative effect is higher than that from the fin neglecting radiative effect. The local heat transfer coefficient with radiative effect is higher than that without radiative effect. In the direction from tip to base, those coefficients decrease at first, attain minimum, and then increase. The larger values of N$_{c}$ M, Pr give rise to larger fin temperature variations and the fin temperature without radiative effect is always higher than that with radiative effect.

• BMB Reports
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• v.39 no.1
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• pp.91-96
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• 2006

Previously, we have shown that nitric oxide (NO) directly activates the Maxi-K channels. In the present study, we have investigated whether NO has prolonged effects on the Maxi-K channels reconstituted in lipid bilayer. Application of S-nitroso-N-acetyl-D, L-penicillamine (SNAP), a NO donor, induced an immediate increase of open probability (Po) of Maxi-K channel in a dose-dependent manner. When SNAP was removed from the cytosolic solution, the Po did not simply returned to, but irreversibly decreased to a level lower than that of the control Po. At 0.2 mM, (Z)-[N-(3-Ammoniopropyl)-N-(n-propyl)amino] diazen-1-ium-1,2-diolate (PAPA-NO), another NO donor, produced a similar increase of Po and decrease of Po upon washout. The increasing effects of SNAP on Po were not blocked by either 50 U/ml superoxide dismutase (SOD) or 2 mM N-ethylmaleimide (NEM) pre-treatments. However, NEM appears to be ineffective when applied after SNAP. These results suggest that NO can modulate Maxi-K channel via direct interaction and chemical modification, such as S-nitrosylation in the brain.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.51 no.6
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• pp.491-496
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• 2006

The present study was initiated to explore the yield potential of bushbean by exploiting the interaction of variety, sowing time, and fertilizers containing N, P, and K Two varieties namely BARI Bushbean 1 and BARI Bushbean 2, three levels of fertilizer viz. control ($N_{0}P_{0}K_{0}$), medium ($N_{20}P_{30}K_{30}$), high ($N_{40}P_{60}K_{60}$) and three sowing times (November 15, December 1 and December 15) were considered as treatment variables. Among the varieties, BARI Bushbean 2 always showed better performance for most of the yield and yield attributes duly attributed by the application of fertilizers ${\circleda}$ N: P: K = 40: 60: 60 respectively. The highest seed yield (1375.17 kg/ha) was recorded when the crop was sown on the $15^{th}$ November with the supplemented soil nutrition status as above. The influence of sowing times indicate that there is a gradual trend of decreasing in seed yield and other associated parameters after first sowing ($15^{th}\;November$). The results of this study suggest that the multi-locational trial under varied sowing times with soil nutrition might potentially increase the long-term adaptation of bushbean in Bangladesh.