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http://dx.doi.org/10.5012/bkcs.2002.23.8.1073

Thermodynamic Elucidation of Binding Isotherms for Hemoglobin & Globin of Human and Bovine upon Interaction with Dodecyl Trimethyl Ammonium Bromide  

Bordbar, A.K.
Nasehzadeh, A.
Ajloo, D.
Omidiyan, K.
Naghibi, H.
Mehrabi, M.
Khajehpour, H.
Rezaei-Tavirani, M.
Moosavi-Movahedi, A.A.
Publication Information
Bulletin of the Korean Chemical Society / v.23, no.8, 2002 , pp. 1073-1077 More about this Journal
Abstract
Binding of dodecyl trimethylammonium bromide (DTAB) to human and bovine hemoglobin and globin samples has been investigated in 50 mM glycine buffer pH = 10, I = 0.0318 and 300 K by equilibrium dialysis and temperature scanning spectrophotometry techniques and method for calculation of average hydrophobicity. The binding data has been analyzed, in terms of binding capacity concept $({\theta})$, Hill coefficient (nH) and intrinsic Gibbs free energy of binding $({\Delta}Gbv).$ The results of binding data, melting point (Tm) and average hydrophobicity show that human hemoglobin has more structural stability than bovine hemoglobin sample. Moreover the results of binding data analysis represent the systems with two and one sets of binding sites for hemoglobin and globin, respectively. It seems that the destabilization of hemoglobin structure due to removal of heme group, is responsible of such behavior. The results indicating the removal of heme group from hemoglobin caused the depletion of first binding set as an electrostatic site upon interaction with DTAB and exposing the hydrophobic patches for protein.
Keywords
Hemoglobin; Globin; Binding sites; Hydrophobicity; Electrostatic contribution;
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