• The Korean Journal of Ecology
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• v.10 no.1
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• pp.23-31
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• 1987

This study was carried out to investigate by the MGP analysis the composition of oribatid mite in coniferous and broad-leaved forests, Kwang Yng experiment plantation, Chola Nam Do Province, southern part of Korea. In these study area, 157 species of oribatid mites (Acari: Cryptogimata) were identified. Among them 6 species such as; Brachychochthonius jugatus JACOT, B hungaricus BALOGH, Eremulus translamealtus BALOGH et. MAHUNKA, Brachioppiella ctenifera GOLOSOVA, Striatoppia opuntiseta BALOGH et. MAHUNKA, Suctobelba perdentata baculifer BALOGH et. MAHUNKA have not been described in Korea. Species compositions were as follows; Among them 69 species were found in all the six sampling sites, 33 species scattered sporadically in all the area, 12 species found in coniferous forest, 10 species in broad-leaved forest only, and 33 species sampled at one site. According to the MGP analysis I, site B-1, B-2, and B-3 were found to be "Type G" and site C-1, C-2, and C-3 were "Type MG". According to the MGP analysis II, site B were found to be all "Type G". But site C-1 was "Type MG", C-2 was "Type M" and C-3 was "Type G" suggesting that "Group M" increased in the site C.e G" suggesting that "Group M" increased in the site C. in the site C.

• Korean Journal of Ecology and Environment
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• v.41 no.1
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• pp.111-115
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• 2008

Direct effects of zooplankton grazing activities on the natural assemblage of bacterioplankton and algae were evaluated at monthly intervals, from June to October of 2000, in the middle part of the River Spree, Germany. We quantified bacterioplankton, algae, zooplankton abundance and measured carbon ingestion rates (CIRs) by zooplankton according to two zooplankton size classes: (i) micro zooplankton (MICZ), ranging in size from 30 to $150{\mu}m$ and including rotifers and nauplii, excluding protozoans and (ii) macrozooplankton (MACZ), larger than $150{\mu}m$ and including cladocerans and copepods. CIRs were measured using natural bacterial and algae communities in the zooplankton density manipulation experiments. Algae biomass (average${\pm}$SD: $377{\pm}306{\mu}gC\;L^{-1}$, n=5) was always higher than bacterial biomass ($36.7{\pm}9.9{\mu}gC\;L^{-1}$, n=5). Total zooplankton biomass varied from 19.8 to $137{\mu}gC\;L^{-1}$. Total mean biomass of zooplankton was $59.9{\pm}52.5{\mu}gC\;L^{-1}$ (average${\pm}$SD, n=5). Average MICZ biomass ($40.2{\pm}47.6{\mu}gC\;L^{-1}$ n=5) was nearly twofold higher than MACZ biomass ($19.6{\pm}20.6{\mu}gC\;L^{-1}$ n=5). Total zooplankton CIRs on algae (average${\pm}$SD: $56.6{\pm}26.4{\mu}gC\;L^{-1}\;day^{-1}$) were $\sim$fourfold higher than that on bacteria $(12.7{\pm}6.0{\mu}gC\;L^{-1}\;day^{-1})$. MICZ CIRs on bacteria $(7.0{\pm}2.8{\mu}gC\;L^{-1}\;day^{-1})$ and algae $(28.6{\pm}20.6{\mu}gC\;L^{-1}\;day^{-1})$ were slightly higher than MACZ CIRs. On average, MICZ accounted for 55.6 and 50.5% of total zooplankton grazing on bacteria and algae, respectively. Considering the MICZ and MACZ CIRs, the relative role of transferring carbon to higher trophic levels were nearly similar between both communities in the lake-river ecosystem.

• Applied Chemistry for Engineering
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• v.23 no.1
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• pp.93-99
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• 2012

In this study, the evaluation of antioxidative activity and componential analysis of C. obtusa leaf extracts was carried out. Reactive oxygen species (ROS) scavenging activities ($OSC_{50}$) of C. obtusa leaf extracts on ROS generated in $Fe^{3+}-EDTA/H_2O_2$ system were investigated using the luminol-dependent chemiluminescence assay. The ethyl acetate fraction ($OSC_{50}$; 0.22 ${\mu}g/mL$) and aglycone fraction of C. obtusa leaf extracts (0.20 ${\mu}g/mL$) showed about 7 times more prominent ROS scavenging activity than L-ascorbic acid (1.50 ${\mu}g/mL$). The cellular protective effects of fractions obtained from C. obtusa leaf extracts on the rose-bengal sensitized photohemolysis of human erythrocytes were investigated. The ethyl acetate fraction and aglycone fraction of C. obtusa leaf extracts showed the cellular protective effects in a concentration dependent manner (5~25 ${\mu}g/mL$). The inhibitory effect ($IC_{50}$) of ethyl acetate fraction and aglycone fraction on tyrosinase exhibited 74.43 and 53.80 ${\mu}g/mL$, repectively. The aglycone fraction showed four times higher tyrosinase inhibitory effect than arbutin (226.88 ${\mu}g/mL$), known as a whitening agent. The aglycone fraction of C. obtusa leaf extracts showed three bands in TLC chromatogram and three peaks in HPLC chromatogram (360 nm). Three compounds were identified as taxifolin, quercetin and kaempferol. These results indicate that the fractions of C. obtusa leaf extracts can function as antioxidants in biological systems, particularly skin exposed to UV radiation by scavenging $^1O_2$ and other ROS, and protect cellular membranes against reactive oxygen species. The fractions of C. obtusa leaf extracts can be applicable to new functional cosmetics for antioxidan and whitening effects.

• KSBB Journal
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• v.27 no.2
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• pp.97-102
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• 2012

In the present study, the biomass productivity of two green microalgae (Chlorella sp. and Dunaliella salina DCCBC2) were assessed in a 12 L tubular photobioreactor under optimum culture conditions. In the batch culture optimization process, the Chlorella sp. biomass was obtained as 1.2 g/L under atmospheric air as a sole $CO_2$ source and other culture conditions as follows: light intensity, temperature, pH, $NH_4Cl$ and $K_2HPO_4$ were 100 ${\mu}E/m^2/s$, $27^{\circ}C$, 7.0, 20.0 mM and 2.0 mM, respectively. On the other hand, 2.9 g/L of D. salina DCCBC2 biomass production was observed under the following conditions: light intensity, temperature, pH, $KNO_3$ and $K_2HPO_4$were 80 ${\mu}E/m^2/s$, $27^{\circ}C$, 8.0, 3.0 mM and 0.025 mM, respectively. At 1% $CO_2$ supply to the reactor, the Chlorella sp. production was reached 1.53 g/L with 25% increment under the same operating conditions. In addition, the maximum D. salina DCCBC2 biomass was observed as 3.40 g/L at 3% $CO_2$ concentration. Based on the aforementioned optimized conditions, the dilution rate and maximal biomass productivity of Chlorella sp. and D. salina DCCBC2 in the continuous cultivation were 0.4/d and 0.6 g/L/d and 0.6/d and 1.5 g/L/d, respectively.

• Microbiology and Biotechnology Letters
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• v.37 no.1
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• pp.42-48
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• 2009

Anticandidial activity of seven herbal extracts, Taraxacum Platycarpum, Houttuyniae Herba, Lonicerae Flos, Anemarrhena Rhizome, Forsythia Fruit, Paeoniae Ratix, and Coptidis Rhizoma, were determined against five different Candida sp. by agar diffusion assay. The concentration of total phenolic compounds of seven herbal extracts ranged from 0.6 to $2.5{\mu}g/mg$. The total antioxidant activities showed that Taraxacum Platycarpum and Houttuyniae Herba were 60% in 80% ethanol extract and Lonicera Flower and Paeoniae Ratix were 70, 75%, respectively, in 100% ethanol extract. Coptidis Rhizoma extract showed antifungal activity against non-Candida albicans, C. tropicalis and C. glabrata. The MIC values of a compound separated in TLC from Coptidis Rhizoma extract were 24, and $48{\mu}g/mL$ against C. tropicalis and C. glabrata. The above compound showed the same retention time with berberin in HPLC analysis.

• Childhood Kidney Diseases
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• v.13 no.2
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• pp.138-145
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• 2009

Purpose : This study was aimed to evaluate the changes of immunologic parameters during hospitalization, and the relationship between IgG and other laboratory or clinical indices in patients with acute poststreptococcal glomerulonephritis (APSGN). Methods : We reviewed the medical charts of 36 children with APSGN who showed ASO titer>250 Todd U/L and C3<70 mg/dL. We evaluated the levels of IgG and other laboratory parameters including C3 and ASO at admission and at discharge (14 cases). Results : The mean age of APSGN patients was $7.5{\pm}2.6$ year of age, and male-to-female ratio was 2.3:1. At presentation, hypertension (systolic blood pressure>125 mmHg), gross hematuria, and weight gain were observed in 27.8% (10/36), 80.1% (29/36), and 80% (24/30) of the patients, respectively. The mean IgG level was $1,432{\pm}322$ mg/dL ($1,025{\pm}234$ mg/dL in control group, P<0.001), and C3 and ASO levels were $26.1{\pm}16.1$ mg/dL and $1,068{\pm}730$ Todd U, respectively. There were no correlation between IgG level and the levels of any of the parameters analyzed (ASO, C3, BUN, creatinine and white blood cell count), and the severity of the disease assessed by the weight-change during admission. The patients aged<6 years of age (10 cases) had less degree of the weight-change, compared to those of the patients aged>8 years of age (15 cases) (-0.6% vs. -5.7%, P=0.01). The IgG and ASO levels did not change, but C3 (P=0.001) and IgM (P=0.02) levels increased during admission. Conclusion : Increased IgG and ASO levels in APSGN did not change, but C3 level increased during admission. IgG level was not correlated with other laboratory parameters (ASO and C3) and the severity of the disease. Younger children seem to have less severe clinical course compare to older children. With our hypothetic pathogenesis of APSGN, further studies are needed to resolve the pathogenesis of the disease including the increase of IgG.

• Communications of Mathematical Education
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• v.5
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• pp.523-523
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• 1997

Suppose that G is a group of permutations of a set ${\Omega}$. For a finite subset ${\gamma}$of${\Omega}$, the movement of ${\gamma}$ under the action of G is defined as move(${\gamma}$):=$max\limits_{g{\epsilon}G}|{\Gamma}^{g}{\backslash}{\Gamma}|$, and ${\gamma}$ will be said to have restricted movement if move(${\gamma}$)<|${\gamma}$|. Moreover if, for an infinite subset ${\gamma}$of${\Omega}$, the sets|{\Gamma}^{g}{\backslash}{\Gamma}| are finite and bounded as g runs over all elements of G, then we may define move(${\gamma}$)in the same way as for finite subsets. If move(${\gamma}$)${\leq}$m for all ${\gamma}$${\subseteq}$${\Omega}$, then G is said to have bounded movement and the movement of G move(G) is defined as the maximum of move(${\gamma}$) over all subsets ${\gamma}$ of ${\Omega}$. Having bounded movement is a very strong restriction on a group, but it is natural to ask just which permutation groups have bounded movement m. If move(G)=m then clearly we may assume that G has no fixed points is${\Omega}$, and with this assumption it was shown in [4, Theorem 1]that the number t of G=orbits is at most 2m-1, each G-orbit has length at most 3m, and moreover|${\Omega}$|${\leq}$3m+t-1${\leq}$5m-2. Moreover it has recently been shown by P. S. Kim, J. R. Cho and C. E. Praeger in [1] that essentially the only examples with as many as 2m-1 orbits are elementary abelian 2-groups, and by A. Gardiner, A. Mann and C. E. Praeger in [2,3]that essentially the only transitive examples in a set of maximal size, namely 3m, are groups of exponent 3. (The only exceptions to these general statements occur for small values of m and are known explicitly.) Motivated by these results, we would decide what role if any is played by primes other that 2 and 3 for describing the structure of groups of bounded movement.

• Annals of Fuzzy Mathematics and Informatics
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• v.16 no.3
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• pp.301-316
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• 2018

In this paper, the concept of connected geodesic number, $gn_c(G)$, of a fuzzy graph G is introduced and its limiting bounds are identified. It is proved that all extreme nodes of G and all cut-nodes of the underlying crisp graph $G^*$ belong to every connected geodesic cover of G. The connected geodesic number of complete fuzzy graphs, fuzzy cycles, fuzzy trees and of complete bipartite fuzzy graphs are obtained. It is proved that for any pair k, n of integers with $3{\leq}k{\leq}n$, there exists a connected fuzzy graph G : (V, ${\sigma}$, ${\mu}$) on n nodes such that $gn_c(G)=k$. Also, for any positive integers $2{\leq}a<b{\leq}c$, it is proved that there exists a connected fuzzy graph G : (V, ${\sigma}$, ${\mu}$) such that the geodesic number gn(G) = a and the connected geodesic number $gn_c(G)=b$.

• Journal of Microbiology and Biotechnology
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• v.25 no.10
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• pp.1742-1750
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• 2015

Human insulin is composed of 21 amino acids of an A-chain and 30 amino acids of a B-chain. This is the protein hormone that has the role of blood sugar control. When the recombinant human proinsulin is expressed in Escherichia coli, a serious problem is the formation of an inclusion body. Therefore, the inclusion body must be denatured and refolded under chaotropic agents and suitable reductants. In this study, H27R-proinsulin was refolded from the denatured form with β-mercaptoethanol and urea. The refolding reaction was completed after 15 h at $15^{\circ}C$, whereas the reaction at $25^{\circ}C$ was faster than that at $15^{\circ}C$. The refolding yield at $15^{\circ}C$ was 17% higher than that at $25^{\circ}C$. The refolding reaction could be carried out at a high protein concentration (2 g/l) using direct refolding without sulfonation. The most economical and optimal refolding condition for human preproinsulin was 1.5 g/l protein, 10 mM glycine buffer containing 0.6 M urea, pH 10.6, and 0.3 mM β-mercaptoethanol at $15^{\circ}C$ for 16 h. The maximum refolding yield was 74.8% at $15^{\circ}C$ with 1.5 g/l protein. Moreover, the refolded preproinsulin could be converted into normal mature insulin with two enzymes. The average amount of human insulin was 138.2 g from 200 L of fermentation broth after enzyme reaction with H27R-proinsulin. The direct refolding process for H27R-proinsulin was successfully set up without sulfonation. The step yields for refolding and enzyme reaction were comparatively high. Therefore, our refolding process for production of recombinant insulin may be beneficial to the large-scale production of other biologically active proteins.

• Food Science and Biotechnology
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• v.14 no.2
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• pp.190-195
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• 2005

The conditions for the extraction of effective substances from the stem of Opuntia ficus-indica were optimized by a response surface methodology. The total extract yield was maximized under the temperature of $97.77^{\circ}C$, at a time duration of 145.82 min and a water to sample ratio 16.59 mL/g. Moreover, the optimum conditions for the extraction of effective substances were as follows: $84.95^{\circ}C$, 156.50 min and a water/sample ratio of 7.46 mL/g for the phenolics content; and $97.11^{\circ}C$, 139.03 min and a water/sample ratio of 10.91 mL/g for the pectin content. The range of optimum extraction conditions in consideration of the physicochemical properties of the extracts were shown to be as $95-100^{\circ}C$ as the extraction temperature, 120-180 min as extraction time and a water to sample ratio of 5-18 mL/g.