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The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Comparison of Biomass Productivity of Two Green Microalgae through Continuous Cultivation

두 종 미세 녹조류의 연속배양을 통한 바이오매스 생산성 비교

  • Gim, Geun-Ho (Department of Environmental Engineering, BK 21 Team for Biohydrogen Production, Chosun University) ;
  • Lee, Young-Mi (Department of Environmental Engineering, BK 21 Team for Biohydrogen Production, Chosun University) ;
  • Kim, Duk-Jin (Department of Water Supply and Sewage, Korea Environment Corporation) ;
  • Jeong, Sang-Hwa (Department of Mechanical Engineering, Chosun University) ;
  • Kim, Si-Wouk (Department of Environmental Engineering, BK 21 Team for Biohydrogen Production, Chosun University)
  • 김근호 (조선대학교 환경공학과, 조선대학교 BK21 바이오수소 생산팀) ;
  • 이영미 (조선대학교 환경공학과, 조선대학교 BK21 바이오수소 생산팀) ;
  • 김덕진 (한국환경공단 상하수도지원처 물산업진흥팀) ;
  • 정상화 (조선대학교 기계공학과) ;
  • 김시욱 (조선대학교 환경공학과, 조선대학교 BK21 바이오수소 생산팀)
  • Received : 2012.01.25
  • Accepted : 2012.03.27
  • Published : 2012.04.30
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Abstract

In the present study, the biomass productivity of two green microalgae (Chlorella sp. and Dunaliella salina DCCBC2) were assessed in a 12 L tubular photobioreactor under optimum culture conditions. In the batch culture optimization process, the Chlorella sp. biomass was obtained as 1.2 g/L under atmospheric air as a sole $CO_2$ source and other culture conditions as follows: light intensity, temperature, pH, $NH_4Cl$ and $K_2HPO_4$ were 100 ${\mu}E/m^2/s$, $27^{\circ}C$, 7.0, 20.0 mM and 2.0 mM, respectively. On the other hand, 2.9 g/L of D. salina DCCBC2 biomass production was observed under the following conditions: light intensity, temperature, pH, $KNO_3$ and $K_2HPO_4$were 80 ${\mu}E/m^2/s$, $27^{\circ}C$, 8.0, 3.0 mM and 0.025 mM, respectively. At 1% $CO_2$ supply to the reactor, the Chlorella sp. production was reached 1.53 g/L with 25% increment under the same operating conditions. In addition, the maximum D. salina DCCBC2 biomass was observed as 3.40 g/L at 3% $CO_2$ concentration. Based on the aforementioned optimized conditions, the dilution rate and maximal biomass productivity of Chlorella sp. and D. salina DCCBC2 in the continuous cultivation were 0.4/d and 0.6 g/L/d and 0.6/d and 1.5 g/L/d, respectively.

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References

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