• KSBB Journal
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• 제13권5호
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• pp.569-576
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• 1998

The effects of carbohydrates, hormones and elicitors on both cell growth and betaine production were investigated in the cell cultures of Lycium chinense Mill. The maximum effect of glucose and sucrose was observed in cells cultured in the presence of 3% and 7% for cell growth and betaine production, respectively. The effect of hormones on cell growth and betaine production was prominent in the presence of 10 ${\mu}$M 2, 4-D, 10 ${\mu}$M NAA and 2.5 ${\mu}$M IAA, whereas cell growth and betaine production were excellent at 2.5 ${\mu}$M BA and 10 ${\mu}$M BA, respectively. Abiotic elicitors such as KCI, MnCl2 and NaCl exhibited an inhibitory role on cell growth in all treatment groups. Betaine production was increased according to increase of concentration of abiotic elicitors. methanol-soluble and insoluble components as biotic elicitor remarkably inhibited cell growth from 2 mg and 6 mg, respectively. Betaine production was increased maximally at 2 mg of biotic elicitors. When growth medium was switched to production medium at two stage culture, it resulted that cell fresh weight and dry weight decreased but betaine content increased about 2.2-fold.

• 한국자원식물학회지
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• 제20권3호
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• pp.251-254
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• 2007

This study was conducted to investigate the optimal plant growth regulator level for the shoot formation of Lycium chinense Mill. In vitro plant propagation was developed for leaf explants of boxthorn. Leaf explants were cultured on MS medium supplemented with cytokinins (BA and 2-iP) alone. Plants were successfully regenerated through in vitro culture by using leaf explants of boxthorn grown in the field. After 4 weeks of culture, 58% of shoot formation had developed from the leaf explants. The shoot formation rate of 'Jangmyeong' was highest followed by 'Myeongan', ;Cheondae', and 'Bullo'. The use of 0.2mg/L BA was critical for enhanced production of shoot formation and resulted in 58% of the culture producing shoot formations. Regenerated plantlets transplanted to pots were developed and successfully acclimatized to greenhouse.

• 한국약용작물학회지
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• 제8권4호
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• pp.291-296
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• 2000

구기자나무의 부위별 물추출물이 LPS (lipopolysaccharide) 및 Con A (concanavalin A)에 의한 마우스의 비장세포 증식능에 미치는 영향을 시험한 결과 부위별 면역 활성중 과실에서 의 No mil 처리구에서는 $0.1mg/ml{\sim}0.5mg/ml$ 농도에서, LPS 처리구에서는 $0.01mg/ml{\sim}0.1mg/ml$의 농도에서 B-cell (체액성 면역)의 분열증식을 촉진시켰다. 잎과 뿌리는 LPS 처리구에서 0.1mg/ml 까지 비장세포의 분열증식을 촉진시켰으며 Con A 처리구에서는 T-cell (세포성 면역)의 증식효과가 없었다.

• 한국자원식물학회지
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• 제7권1호
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• pp.23-27
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• 1994

The growing condition and the point at issue were investigated for utilization as a fundamental data of the Boxthorn research in the growing farm household, which was chief producing district of Box-thorn in Chongyang, Chungmam province. The results were summarized as follow'; Lycium fruit wasproduced 445M/. in the 170.4ha. In practical cultivation, major numbers of decapitation were threetimes,, and planting distance was dense planting, and also amount of applied fertilizer was beyondbasic-fertilizer level and number of split application was abundant.Numbers of harvesting were six-ten times, and many labor was requiremented.The issues in cultivation were development of processing technique, supplement of varieties whichhave disease tolerance, superior quality and high yielding, improving of cultivation methods for laborsaving and magnification of demand.

• Journal of Biosystems Engineering
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• 제24권4호
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• pp.365-372
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• 1999

Physical characteristics of stem (ile., length and diameter) and branch, and detachment force of mature and immature fruits of 12 species of the Lycium chinese Mill at a cultivar were determined. The number of stems and branches were counted after the first and second pruning of each plant. Length and diameter of the stem were average of 113.5 cm and 9.5 mm for 12 species of the Lycium chinese Mill, respectively. Average number of stem and number of branch after the first and second pruning in each plant were 5, 30, and 61, respectively. Diameter of major and minor axis of the fruit was average of 13.8mm and 8.3mm, respectively, and the sphericity of fruits was average of 0.7 for 12 species of Lycium chinese Mill. Detachment force of mature and immature fruits was average of 1 N and 2.7 N, respectively, for 12 species of the Lycium chinese Mill. The maximum and minimum detachment force of the mature fruits was 2.06 N and 0.39 N, respectively, and that of the immature fruits was 3.72 N and 1.27 N, respectively. The force-weight ratio showed a decreasing trend as the weight of fruit increasing for all samples.

• 한국농업기계학회:학술대회논문집
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• 한국농업기계학회 1999년도 동계 학술대회 논문집
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• pp.139-148
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• 1999

• 한국농업기계학회:학술대회논문집
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• 한국농업기계학회 2000년도 동계 학술대회 논문집
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• pp.300-308
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• 2000

• 식물조직배양학회지
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• 제28권6호
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• pp.329-333
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• 2001

배지, 절편체의 종류 그리고 생장조절제가 약용작물인 구기자나무 (Lycium chinese Mill. 'Cheongyang')의 캘러스 유도 및 신초 분화조건에 미치는 영향을 조사하였다. 배지 종류별 기관분화는 신초 분화율, 신초 발생수 및 생체중이 MS, B5, WPM 배지 순으로 양호하였다. 구기자나무의 캘러스 유도는 절간 절편체보다 잎 절편체가 좋았고, 캘러스는 0.5mg/L NAA와 0.2 mg/L BA가 첨가된 MS배지에서 효율적으로 유도되었다. 신초 분화에 관여하는 cytokinin으로는 TDZ보다는 BA가 좋았으며 BA 단일처리보다는 NAA와의 혼용처리에서 더 양호하였다. 신초분화는 0.01 mg/L NAA와 0.2 mg/L BA가 첨가된 MS 배지에서 가장 좋았다

• 한국식물병리학회지
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• 제2권1호
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• pp.31-36
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• 1986

구기자(Lycium chinense Mill) 탄저병은 구기자 열매에 수침상의 흑색 병반을 형성하고 포자층을 형성한다. 일반농가 포장에서의 발병율은 $0.8\%\~23\%$(평균 $9.6\%$)의 이병과율을 보였다. 구기자 탄저병균은 Colletotrichum gloeosporioides Penz와 Colletotrichum dematium(Fr.) Grove의 2종으로 동정되었다. 구기자에 대한 병원성은 공시한 C. gloeosporioides와 C. dematium 모두 어린 잎이나 줄기에는 병원성이 없었고 열매에는 강한 병원성을 나타냈다. 성숙과에는 C. dematium Isolate I이 특히 병원성이 강하였고 미숙과에 대하여는 C. dematium Isolate II만이 약하게 나타났으나 10일정도 경과하면 병원성 정도의 구분이 어려웠다.

• Journal of Biosystems Engineering
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• 제26권2호
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• pp.105-114
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• 2001

Modulus of elasticity, modulus of rigidity, damping ratio, and natural frequency of three varieties of boxthorn (Lycium chinense Mill) (Cheongyang #2, Cheongyang gugija, and Cheongyang native) branches were analyzed. Modulus of elasticity and modulus of elasticity and modulus of rigidity of the boxthorn branch was determined using standard formula after simple beam bending and torsion test, respectively, using an universal testing machine. Damping ratio and natural frequency of branches were determined using a system consisted of an accelerometer, a PC equipped with A/D converter, and a software for data analysis. Relationship between the elastic modulus and branch diameter in overall varieties and branch types showed a good correlation (r -0.81). There was, however, no correlation between torsional rigidity and branch diameter. The internal damping results were highly variable and the overall range of the damping ratio of the boxthorn branch was 0.014-0.087, which indicated that the branch was a lightly damped structure. The natural frequency of the boxthorn branch was in the range of 89-363 rad/s for the overall varieties and branch types. A good correlation (r 0.82) existed between the natural frequency and branch diameter in overall varieties and branch type.