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Plant Regeneration through Leaf Explant Culture of Boxthorn (Lycium chinense Mill.)  

Ham, In-Ki (Chungcheongnam-do Agricultural Research & Extension Services)
Park, Sang-Kyu (Chungcheongnam-do Agricultural Research & Extension Services)
Lee, Bong-Chun (Chungcheongnam-do Agricultural Research & Extension Services)
Lee, Mi-Ae (Chungcheongnam-do Agricultural Research & Extension Services)
Kwon, Kyeong-Hak (Chungcheongnam-do Agricultural Research & Extension Services)
Lee, Eun-Mo (Chungcheongnam-do Agricultural Research & Extension Services)
Jo, Man-Hyun (Chungcheongnam-do Agricultural Research & Extension Services)
Publication Information
Korean Journal of Plant Resources / v.20, no.3, 2007 , pp. 251-254 More about this Journal
Abstract
This study was conducted to investigate the optimal plant growth regulator level for the shoot formation of Lycium chinense Mill. In vitro plant propagation was developed for leaf explants of boxthorn. Leaf explants were cultured on MS medium supplemented with cytokinins (BA and 2-iP) alone. Plants were successfully regenerated through in vitro culture by using leaf explants of boxthorn grown in the field. After 4 weeks of culture, 58% of shoot formation had developed from the leaf explants. The shoot formation rate of 'Jangmyeong' was highest followed by 'Myeongan', ;Cheondae', and 'Bullo'. The use of 0.2mg/L BA was critical for enhanced production of shoot formation and resulted in 58% of the culture producing shoot formations. Regenerated plantlets transplanted to pots were developed and successfully acclimatized to greenhouse.
Keywords
Leaf; shoot formation; Lycium chinense Mill;
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Times Cited By KSCI : 2  (Citation Analysis)
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1 Chungcheongnam-do Agricultural Research & Extension Services. 2000b. Research report. pp. 425-432
2 Chungcheongnam-do Agricultural Research & Extension Services. 2004. Research report. pp. 373-380
3 Jo, M.H., I.K. Ham, B.C. Lee, J.W. Kim, W.S. Lee, S.Y. Kwon, H.S. Lee and S.S. Kwak. 2004. High frequency shoot formation and plant regeneration from cotyledonary hypocotyl explants of boxthorn (Lycium chinense Mill.) seedlings. Korean J. Plant Biotechnol. 31: 203-207   DOI
4 Kim S.M. 1997. Agronomic characteristics and changes in chemical components as affected by harvest time in Lycium chinense Mill. Department of Agronomy Graduate School, Dankook University Ph.D. Thesis
5 Murashige, T. and F. Skoog. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant 15: 473-497   DOI
6 Tang, W. and G. Eisenbrand. 1986. Chinense drugs of plant origin. Springer-Verlag. pp. 633-638
7 Kim, D.C., H.J. Chung, B.H. Min and D.C. Yang. 2001. Plant regeneration from leaf and internode segment cultures of boxthorn (Lycium chinense Mill.). Korean J. Plant Biotechnol. 28: 329-333   과학기술학회마을
8 Kurokawa S. 1962. General pharmacological studies of the water and various organic solvent soluble components derived from Lycium chinense. Shikoku I gkuzasshi. 18: 127-136
9 Park, Y.G., B.W. Kim, M. S. Choi and K.S. Noh. 1993. In vitro organogenesis from leaf callus of Lycium chinense Mill. Korean J. Plant Tissue Culture 20: 85-89
10 Chungcheongnam-do Agricultural Research & Extension Services. 2000a. Research report. pp. 417-424
11 Chungcheongnam-do Agricultural Research & Extension Services. 1997. Research report. pp. 773-776
12 Hotta, M., K. Ogata, A. Nitta, K. Hosikawa, M. Yanagi and K. Yamazaki. 1989. Sekai yuhyou-shokubutu jiten (useful plant of the world). Heibonsha, Tokyo. pp. 640-641
13 Lee, B.C., J.S. Park, T.S. Kwak and C.S. Moon. 1998. Variation of chemical properties in collected boxthorn varieties. Korean J. Breed. 30: 267-272
14 Lee, B.C., S.D. Kim, T.S. Yun, B.H. Lee, S.W. Ra, Y.C. Park, I.S. Woo, D.H. Kim and T.S. Jeon. 2000. Production of new cultivar in boxthorn (Lycium chinense Mill.). Research report of Chungcheongnam-do Agricultural Research & Extension Services, Yeasan, Korea. pp. 417-424
15 Lee, M.S., D.C. Kim, J.H. Kim and W.J. Lim. 1984. Studies on the tissue culture of Lycium chinense Mill. Bul. Agr. Wonkwang Univ. 7: 261-275