• 한국식품영양과학회지
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• 제32권7호
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• pp.1108-1113
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• 2003

본 연구에서는 방사선 손상 경감효과를 나타내는 기호식품을 발굴하고자 국내에서 주로 음용되고 있는 녹차의 방사선 손상 경감효과를 확인하기 위하여 고선량 및 저선량 방사선을 마우스에 조사하고 소장움 생존, 내재성 비장 조혈세포집락 형성 및 apoptosis 유발 등을 관찰하였으며, 그 효과를 방사선 방호효과가 알려져 있는 diethyldithiocarbamate(DDC)와 비교$.$관찰하였다. 생존 소장움의 수는 방사선 조사 전 녹차 투여군(경구투여군 : p<0.01, 복강내 투여군 : p<0.01) 및 DDC 투여군(p<0.005)에서 유의성있게 증가되었다. 내재성 조혈세포집락 형성은 방사선 조사 대 조군에 비하여 조사 전 복강내 투여한 군(p<0.05)에서만 유의성 있는 증가를 나타냈으며, DDC 투여군에서는 효과를 관찰할 수 없었다. 방사선에 의한 apoptotic cell의 형성은 방사선 조사 전 녹차 투여군(경구 투여군 : p<0.005, 복강내 투여군 : p<0.05)및 DDC투여군 (p<0.005)에서 통계적으로 유의성 있게 감소되었다. 이상의 결과에서 녹차의 방사선 손상 경감효과를 조혈세포의 생존과 회복, 소장움 세포 생존 및 방사선에 의한 apoptosis 유발억제를 통하여 확인하였으며, 이는 독성이 적은 천연물이라는 관점에서 방사선 손상 경감 음료로서 적용이 가능할 것으로 사료되며 추후 유효 분획 및 성분에 관한 연구가 요구된다.

• 한국방사선학회논문지
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• 제5권6호
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• pp.415-420
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• 2011

본 연구는 방사선 일반 촬영시 촬영 도움자가 피폭받을 수 있는 테이블 주변의 산란선에 의한 피폭선량을 알아보고자 하였다. 두개부, 흉부, 복부, 요추부, 고관절, 그리고 슬관절 촬영시 테이블 중앙으로부터 옆으로 45cm, 75cm 떨어진 지점에서 바닥으로부터 70cm, 80cm, 130cm, 150cm 높이에서 산란선에 의한 피폭선량을 측정하였다. 측정결과 요추 측방향, 복부 정면, 그리고 고관절 촬영이 80cm 높이에서 $66.21{\mu}Sv$, $34.22{\mu}Sv$와 $32.35{\mu}Sv$로 가장 높은 피폭을 보였고, 흉부, 복부, 고관절, 두개부, 슬관절 순서로 선량이 감소하였다. 촬영 테이블 높이는 70cm 보다 80cm에서 대체로 높은 선량을 보였으며, 80cm 이상에서는 높이가 높아질수록 선량은 감소하였다. 측정된 선량들은 촬영 도움자가 납 방호복을 착용할 경우 일반인 연간 피폭선량한도인 1mSv와는 큰 차이가 있어 우려할 선량은 아닌 것으로 나타났다.

• 대한수의학회지
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• 제27권2호
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• pp.185-189
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• 1987

The number of splenic lymphocyte, serum prostaglandin $E_2$ level and natural killer cell activity were assayed after single whole body irradiation of a sublethal dose of $^{60}Co-{\gamma}$ ray to C57BL/6J mice. With a view to knowing the relationships between radiation induced prostaglandin $E_2$ level and the normal natural killer cell activity after natural killer cell-target cell conjugation, The change of normal natural killer cell activity were measured by administration of prostaglandin $E_2$ containing serum from irradiated mice. The results were summarized as follows; 1. The total number of splenic lymphocyte was significantly decreased by irradiation and the number was not affected by indometacin, prostaglandin synthesis inhibitor, treatment. 2. Serum prostaglandin $E_2$ level was increased in irradiated mice, but indometacin treated mice group showed low level of prostaglandin $E_2$. 3. In the case of irradiated mice, natural killer cell activity was not shown any difference between irradiated group and indometacin combined group. But when natural killer cell-target cell conjugations were exposed to the serum of each group during cytotoxic activity assay, whereas the normal natural killer cell activity was significantly decreased by treatment of serum from irradiated mice, the activity was not changed by treatment of indometacin pretreated mice serum. This result indicated that the prostaglandin $E_2$ induced by the radiation inhibited the post-target binding cytolytic process of natural killer activity.

• Asian Pacific Journal of Cancer Prevention
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• 제14권4호
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• pp.2557-2561
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• 2013

Background: Chemoradiation (CRT) using cisplatin-based regimens has become the standard of care in the treatment of squamous cell head and neck cancers (SCHNC). The impact of taxanes as radiosensitizing agents with concurrent CRT regimens is unknown. We therefore retrospectively evaluated the efficacy and tolerability of a weekly cisplatin+docetaxel combination with CRT in locally advanced SCHNC. Methods: Sixty-six patients with locally advanced SCHNC (39.4% stage IV, 53% stage III, and 7.6% stage II) were assessed retrospectively. Total radiation dose to the PTV of gross disease (primary and/or node) was 70 Gy/35 fractions, 5 fractions per week. Minimum doses of 60 Gy and 50 Gy were administered to PTVs of elective high risk and low risk disease, respectively. Chemotherapy (CT) consisted of weekly cisplatin (20 $mg/m^2$)+docetaxel (20 $mg/m^2$) concurrently with RT. Results: The median age of the patients was 58 years (range, 32-77). Objective response rate was 83.3%. The 2-year progression-free survival (PFS) and overall survival (OS) were 75.7% and 78.3%, respectively. The most common grade 3 and 4 toxicities were mucositis (36.4%), nausea and vomiting (12.1%), neutropenia (4.5%). Conclusion: Weekly cisplatin and docetaxel concurrent with RT for locally advanced SCHNC was found tolerable with high efficacy.

• 대한수의학회지
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• 제43권1호
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• pp.49-55
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• 2003

This study was performed to determine the effect of Phyllostachys nigra var. henenis Strapf leaf extract on jejunal crypt survival, endogenous spleen colony formation and apoptosis in jejunal crypt cells of mice irradiated with high and low dose of gamma-radiation. Phyllostachys nigra var. henenis Strapf administration before irradiation (I.P.: 125 mg/kg of body weight, at 24 hours before irradiation) resulted in an increase of the formation of endogenous spleen colony (p<0.01). The frequency of radiation-induced apoptosis was also reduced by pretreatment of Phyllostachys nigra var. henenis Strapf (I.P.: 280 mg/kg or 28 mg/kg of body weight, at 24 hours before irradiation, p<0.01). These results indicated that Phyllostachys nigra var. henenis Strapf might be a useful radioprotector, especially since it is a relatively nontoxic natural product. Further studies are needed to characterize better the promotion nature of Phyllostachys nigra var. henenis Strapf and its components.

• 한국의학물리학회지:의학물리
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• 제27권3호
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• pp.105-110
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• 2016

Sparse angular sampling has been studied recently owing to its potential to decrease the radiation exposure from computed tomography (CT). In this study, we investigated the analytic reconstruction algorithm in sparse angular sampling using the sinogram interpolation method for improving image quality and computation speed. A prototype of the spectral CT system, which has a 64-pixel Cadmium Zinc Telluride (CZT)-based photon-counting detector, was used. The source-to-detector distance and the source-to-center of rotation distance were 1,200 and 1,015 mm, respectively. Two energy bins (23~33 keV and 34~44 keV) were set to obtain two reconstruction images. We used a PMMA phantom with height and radius of 50.0 mm and 17.5 mm, respectively. The phantom contained iodine, gadolinium, calcification, and lipid. The Feld-kamp-Davis-Kress (FDK) with the sinogram interpolation method and Maximum Likelihood Expectation Maximization (MLEM) algorithm were used to reconstruct the images. We evaluated the signal-to-noise ratio (SNR) of the materials. The SNRs of iodine, calcification, and liquid lipid were increased by 167.03%, 157.93%, and 41.77%, respectively, with the 23~33 keV energy bin using the sinogram interpolation method. The SNRs of iodine, calcification, and liquid state lipid were also increased by 107.01%, 13.58%, and 27.39%, respectively, with the 34~44 keV energy bin using the sinogram interpolation method. Although the FDK algorithm with the sinogram interpolation did not produce better results than the MLEM algorithm, it did result in comparable image quality to that of the MLEM algorithm. We believe that the sinogram interpolation method can be applied in various reconstruction studies using the analytic reconstruction algorithm. Therefore, the sinogram interpolation method can improve the image quality in sparse-angular sampling and be applied to CT applications.

• 대한수의학회지
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• 제40권2호
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• pp.221-228
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• 2000

We performed this study to determine the effect of Bu-Zhong-Yi-Qi-Tang, as a prescription of traditional Oriental medicine, and its major ingredients on jejunal crypt survival, endogenous spleen colony formation, apopotosis in jejunal crypt cells, lethality and hematological change of mice irradiated with high and low dose of Y-radiation. Bu-Zhong-Yi-Qi-Tang administration before irradiation protected the jejunal crypts (p<0.0001), increased the formation of endogenous spleen colony (p<0.05) and reduced the frequency of radiation-induced apoptosis (p<0.05). The survival rate and mean survival time of the groups treated with Bu-Zhong-Yi-Qi-Tang within 30 days after the treatment were far better than the irradiation control group. In the experiment on the effect of ingredients of Bu-Zhong-Yi-Qi-Tang, the result indicated that the extract of Rensan (Panax ginseng), Danggui (Angelica sinensis), Shengma (Cimicifuga heracleifolia) and Chaihu (Bupleurum falcatnosa) might have a major radioprotective effect. Although the mechanisms of this inhibitory effect remain to be elucidated, these results indicated that BU-Zhong-Yi-Qi-Tang might be a useful radioprotector, especially since it is a relatively nontoxic natural product. Further studies are needed to characterize better the protective nature of Bu-Zhong-Yi-Qi-Tang extract and its ingredients.

• Journal of Ginseng Research
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• 제22권1호
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• pp.66-72
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• 1998

Studies were performed to determine the effect of Korean red ginseng (extract powder, spray-dried), it is made of choice 6-year-old raw ginseng roots, and processed by steaming and drying, on jejunal crypt survival, endogenous spleen colony formation, and apoptosis in jejunal crypt cells of irradiated mice. Jejunal crypts were protected by pretreatment of red ginseng (1 mg/head, single I.P. at 24hours before irradiation, p<0.05). Red ginseng administration before irradiation (1 mg/head, single I.P at 24hours before irradiation) resulted in an increase of the formation of endogenous spleen colony (p<0.05). The frequency of radiation-Induced apoptosis in intestinal crypt cells was also reduced by treatment of red ginseng both pretreatment (P.O.: 2 mg/ml of drinking water for 7 days, p<0.005, I.P.: 1 mg/head, single I.P. at 24 hours before irradiation, p<0.005) and post-treatment (1 mg/head, single I.P at 30 minutes after irradiation, p<0.05). These results indicated that Korean red ginseng might be a useful radio-protector, especially since it is a relatively nontoxic natural product. Further studies are needed to characterize better the promotion nature of red ginseng and its fractions.

• 미생물학회지
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• 제6권4호
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• pp.122-130
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• 1968

Action of various wavelengths of visible light on ultraviolet-radiation damage to haploid yeast cells, Saccharomyces cerevisiae 23971, was studied. The results were obtained on the basis of the survival and respiration rates by pre- and post-illuminations of various wavelengths before and after U.V.-irradiations on the yeast cells. Among the wavelengths tested, 635 $m{\mu}$, 429 $m{\mu}$ and white light which caused increase of respiration in pre-treatment alone, induced less resistance to the U. V.-damage than in the control, in both pre- and U.V.-treatment. On the contrary, such wavelengths as 574 $m{\mu}$and 530 $m{\mu}$, showing a weak effect on respiration in pre-treatment increased the susceptability to U.V.-radiation. Photoinactivation was generally obtained by both pre- and post- illuminations along with U.V.-treatment. At 635 $m{\mu}$ the PI rate was the lowest and also a low PI rate was shown at 429 $m{\mu}$. But 429 $m{\mu}$, in the post-treatment of the yeast cells pre-treated by the white light and the darkness respectively, showed the highest PI rate. In both pre- and post- treatment of 574, 530 and 473 $m{\mu}$,the PI rates were high to the same degree. Post-treatments of the wavelengths on U.V.-treated yeasts incubated rather under the white light than the darkness induced lower PI rate. It is assumed that there are great differences in action even of the same wavelength, depending upon the various combination of pre- and post-treatments, and that, moreover, the action of various wavelengths of visible light on U.V.-damage on the cells are concerned with the doses and dose rates of U.V. and visible lights. These observations led to an interpretation that each wavelength of visible light might exert distinctively different effects oil U. V.-damage, mainly causing the inhibition or stimulation of enzymes in the yeast cells.

• 환경생물
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• 제23권1호
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• pp.21-26
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• 2005

Cell response to genotoxic agents is complex and involves the participation of different classes of genes including cell cycle control, DNA repair and apoptosis. In this report, we presented a approach to characterize the cellular functions associated with the altered transcript profiles of MCF-7 exposed to low-dose in vitro gamma-irradiation. We used the method of human 2.4 k cDNA microarrays containing apoptosis, cell cycle, chromatin, repair, stress and chromosome genes to analyze the differential gene expression characterization that were displayed by radiation-exposed cell, human breast carcinoma MCF-7 cell line, such as 4 Gy 4 hr, 8 Gy 4 hr, and 8 Gy 12 hr. Among these genes, 66 were up-regulated and 49 were down-regulated. Specific genes were concomitantly induced in the results. Cyclin dependent kinase 4 (Cdk4) is induced for starting the cell cycle. This regulation is required for a DNA damage­induced G1 arrest. In addition to, an apoptotic pathways gene Bcl-w was concomitantly induced. Mismatch repair protein homologue-l (hMLH1), a necessary component of DNA mismatch protein repair (MMR), in G2-M cell cycle checkpoint arrest. The present study provides new information on the molecular mechanism underlying the cell response to genotoxic stress, with relevance to basic and clinical research.