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A DNA-Damage Response Gene Expression Analysis in MCF-7 followed by γ-Radiation  

Park Ji-Yoon (Department of Biochem & Mol. Biol, Hanyang University)
Hwang Chang-Il (Department of Biochem, Seoul National University)
Park Woong-Yang (Department of Biochem, Seoul National University)
Kim Jin-Kyu (RI Radiation Research Team, Korea Atomic Energy Research Institute)
Chai Young Gyu (Department of Biochem & Mol. Biol, Hanyang University)
Publication Information
Korean Journal of Environmental Biology / v.23, no.1, 2005 , pp. 21-26 More about this Journal
Abstract
Cell response to genotoxic agents is complex and involves the participation of different classes of genes including cell cycle control, DNA repair and apoptosis. In this report, we presented a approach to characterize the cellular functions associated with the altered transcript profiles of MCF-7 exposed to low-dose in vitro gamma-irradiation. We used the method of human 2.4 k cDNA microarrays containing apoptosis, cell cycle, chromatin, repair, stress and chromosome genes to analyze the differential gene expression characterization that were displayed by radiation-exposed cell, human breast carcinoma MCF-7 cell line, such as 4 Gy 4 hr, 8 Gy 4 hr, and 8 Gy 12 hr. Among these genes, 66 were up-regulated and 49 were down-regulated. Specific genes were concomitantly induced in the results. Cyclin dependent kinase 4 (Cdk4) is induced for starting the cell cycle. This regulation is required for a DNA damage­induced G1 arrest. In addition to, an apoptotic pathways gene Bcl-w was concomitantly induced. Mismatch repair protein homologue-l (hMLH1), a necessary component of DNA mismatch protein repair (MMR), in G2-M cell cycle checkpoint arrest. The present study provides new information on the molecular mechanism underlying the cell response to genotoxic stress, with relevance to basic and clinical research.
Keywords
irradiation; MCF-7; DNA-damage response; gene expression;
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