• Fisheries and Aquatic Sciences
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• v.24 no.4
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• pp.163-170
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• 2021

Peptides are naturally occurring biological molecules that are found in all living organisms. These biologically active peptides play a key role in various biological processes. The aim of this study is the extraction and the purification of bioactive materials that induce relaxation of an apical muscle from the pyloric caeca of Patiria pectinifera. The acidified pyloric caeca extract was partially separated by the solid phase extraction using a stepwise gradient on Sep-Pak C18 cartridge. Among the fractions, materials eluted with 60% methanol/0.1% trifluoroacetic acid was put a thorough of a series of high performance liquid chromatography (HPLC) steps to isolate a neuropeptide with relaxation activity. The purified compound was eluted at 28% acetonitrile in 0.1% trifluoroacetic acid with retention time of 25.8 min on the CAPCELL-PAK C18 reversed-phase column. To determine the molecular weight and the amino acid sequence of the purified peptide, LC-MS and Edman degradation method were used, respectively. The primary structure of the peptide was determined to be FGMGGAYDPLSAGFTD which corresponded to the amino acid sequence of a starfish myorelaxant peptide (SMP) isotype (SMPb) found in the cDNA sequence encoding SMPa and its isotypes. In this study, a muscle relaxant neuropeptide (SMPb) has been isolated from pyloric caeca of starfish P. pectinifera. This is the first report of SMPb isolation on the protein level from P. pectinifera.

• Journal of the Society of Disaster Information
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• v.18 no.1
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• pp.163-172
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• 2022

Purpose: To secure efficient thermal management technology for Li-ion batteries, the applicability of the system applied with single-phase immersion technology was checked through an experiment. Method: Using JH3 pouch cells produced by LG-Chem, Korea, A 14S2P module was manufactured and immersed in a vegetable-based cooling fluid produced by Cargill, USA, and then charged and discharged at a rate of 0.3C to 1C to check the heat distribution. Result: It was possible to manage and there was no change in the molecular structure of the immersion solution. Conclusion: It was confirmed that the immersion cooling method can be applied to the thermal management of Li-ion batteries.

• Current Photovoltaic Research
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• v.9 no.4
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• pp.137-144
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• 2021

In this study, the microstructure of the CVD-fabricated Cu(In,Ga)Se₂ (CIGSe) absorber layer by simulating the stacking sequence used in a co-evaporation method, and changes solar cell performance were investigated. The absorber layer prepared by stacking CuSe and (In,Ga)Se between InSe is separated into Ga-free CuInSe₂ and Ga-rich CIGSe, and transformed to CIGSe by selenization heat treatment with slight improvement in the the solar cell efficiency. However, in CVD, since the supply of liquid Cu-Se is not as active as in the co-evaporation method, the nanoocrystalline layer containing a large amount of Ga remained independently in the absorption layer, which acted as a cause of the loss of J_SC and FF. Therefore, by using a precursor structure in which CuGa is sputter-deposited on a single layer of InSe deposited by CVD, performance parameters of V_OC, J_SC, and FF could be greatly improved.

• Proceedings of the Korean Society of Near Infrared Spectroscopy Conference
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• 2001.06a
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• pp.3114-3114
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• 2001

During the last years phytochemistry and phytopharmaceutical applications have developed rapidly and so there exists a high demand for faster and more efficient analysis techniques. Therefore we have established a near infrared transflectance spectroscopy (NIRS) method that allows a qualitative and quantitative determination of new polyphenolic pharmacological active leading compounds within a few seconds. As the NIR spectrometer has to be calibrated the compound of interest has at first to be characterized by using one or other a combination of chromatographic or electrophoretic separation techniques such as thin layer chromatography (TLC), high performance liquid chromatography (HPLC), capillary electrophoresis (CE), gas chromatography (GC) and capillary electrochromatography (CEC). Both structural elucidation and quantitative analysis of the phenolic compound is possible by direct coupling of the mentioned separation methods with a mass spectrometer (GC-MS, LC-MS/MS, CE-MS, CEC-MS) and a NMR spectrometer (LC-NMR). Furthermore the compound has to be isolated (NPLC, MPLC, prep. TLC, prep. HPLC) and its structure elucidated by spectroscopic techniques (UV, IR, HR-MS, NMR) and chemical synthesis. After that HPLC can be used to provide the reference data for the calibration step of the near infrared spectrometer. The NIRS calibration step is time consuming, which is compensated by short analysis times. After validation of the established NIRS method it is possible to determine the polyphenolic compound within seconds which allows to raise the efficiency in quality control and to reduce costs especially in the phytopharmaceutical industry.

• Natural Product Sciences
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• v.28 no.4
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• pp.181-186
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• 2022

A germacrane-type sesquiterpenoid was isolated and purified from a methanol extract of the roots of Valeriana fauriei (RVF) through open column chromatography using silica gel. This compound was verified to be heishuixiecaoline A by spectroscopic analysis. This compound was isolated for the first time from RVF. Quantitative analysis of heishuixiecaoline A from RVF cultivated from three different regions (Eumseong, Jinbu, and Jinan regions) was performed by combining high-performance liquid chromatography with a photodiode array detector. The extract of RVF cultivated in the Jinbu region showed the highest content (9.23 mg/g). In addition, a significant amount of the compound was detected in all RVF samples, which could be expected since it is a characteristic compound of RVF. The sesquiterpenoid group heishuixiecaoline A was isolated from RVF, a resource for various pharmacological substances, and quantitative analysis of RVF cultivated from three different regions was performed. As a result of these experiments, basic data on RVF that can be used in the development and application of pharmaceuticals and health functional foods in the future were obtained.

• Progress in Superconductivity and Cryogenics
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• v.12 no.1
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• pp.37-41
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• 2010

To limit fault current in a power system, superconducting fault current limiters (SFCLs) using high temperature superconducting (HTS) coils have been developed by many research groups so far. Non-inductive winding of HTS coils used for SFCLs can be classified into solenoid winding and pancake winding. Each of winding is expected to have different quench and recovery characteristics because the structure of solenoid winding differs from pancake winding's. Therefore it is important to the SFCLs application to investigate characteristics of each winding. In this paper, we deal with quench and recovery characteristics of four kinds of winding : solenoid winding, pancake winding without spacers, and with spacers of 2 and 4 mm thickness. In order to obtain quench and recovery parameters of coils, short circuit tests were performed in liquid nitrogen.

• Membrane and Water Treatment
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• v.13 no.2
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• pp.63-72
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• 2022

Choline chloride-glycerol (1:2 mol), a natural deep eutectic solvent (NADES) is examined as a draw solution in forward osmosis (FO) for dewatering application. The NADES is easy to prepare, low in toxicity and environmentally benign. A polyamide thin film composite membrane was used. Characterization of the membrane confirmed porous membrane structure with good hydrophilicity and a low structural parameter (722 ㎛) suitable for FO application. A dilute solution of 20% (v/v) NADES was enough to generate moderate water flux (14.98 L m^-2h^-1) with relatively low reverse solute flux (0.125 g m^-2h^-1) with deionized water feed. Application in dewatering industrial wastewater feed showed reasonably good water flux (11.9 L m^-2h^-1) which could be maintained by controlling the external concentration polarization and fouling/scaling mitigation via simple periodic deionized water wash. In another application, clarified sugarcane juice could be successfully concentrated. Recovery of the draw solute was accomplished easily by chilling utilizing thermo responsive phase transition property of NADES. This study established that low concentration NADES can be a viable alternative as a draw solute for dewatering of wastewater and other heat sensitive applications along with a simple recovery process.

• Journal of ILASS-Korea
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• v.28 no.2
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• pp.97-104
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• 2023

A sprinkler is a fire suppression system that extinguishes combustible materials in the early stages of a fire, creating a spray. However, spray formation method of the sprinkler can result in an uneven distribution of water spray on the surface of combustible materials. It is necessary to ensure a consistent water flux density regardless of the spray direction and angle. In this study, the water flux distribution was analyzed for the various types of sprinkler head: circular, flush, pendent, and upright types. All sprinkler heads have a K-factor of 80 LPM/(0.1MPa)^0.5. In this study, water collection cubes were used to examine the water flux distribution. The upright type sprinkler head showed a low standard deviation in total sprayed area, indicating a high level of uniformity. The upright type head showed the lowest standard deviation in the radial direction, and also showed the lowest standard deviation in the azimuthal direction. Upright sprinkler head has no obstructing structure along the path of droplets after they are generated. For this reason, upright sprinkler head showed the most uniform water flux distribution on the floor.

• Nuclear Engineering and Technology
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• v.55 no.7
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• pp.2642-2649
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• 2023

A real-time unmeasured dynamic response prediction process for the nuclear power plant pressure pipeline is proposed and its performance is tested in the test-loop system (KAERI). The aim of the process is to predict unmeasurable or unreachable dynamic responses such as acceleration, velocity, and displacement by using a limited amount of directly measured physical responses. It is achieved by combining a well-constructed finite element model and robust inverse force identification algorithm. The pressure pipeline system is described by using the displacement-pressure vibro-acoustic formulation to consider fully filled liquid effect inside the pipeline structure. A robust multiphysics modal projection technique is employed for the real-time sensor synchronized prediction. The inverse force identification method is also derived and employed by using Bathe's time integration method to identify the full-field responses of the target system from the modal domain computation. To validate the performance of the proposed process, an experimental test is extensively performed on the nuclear power plant pressure pipeline test-loop under operation conditions. The results show that the proposed identification process could well estimate the unmeasured acceleration in both frequency and time domain faster than 32,768 samples per sec.

• Elastomers and Composites
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• v.58 no.3
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• pp.121-127
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• 2023

The effect of diisocyanate type on the decomposition temperature of polyurethane (PU) hydrolysis was investigated in a subcritical water medium up to 250℃. PU samples were prepared using different types of diisocyanate: two aromatic diisocyanates (4,4'-methylene diphenyl diisocyanate (MDI) and methyl phenylene diisocyanate (TDI)), one unbranched aliphatic diisocyanate (hexamethylene diisocyanate (HDI)), and two cyclic aliphatic diisocyanates (4,4'-methylene dicyclohexyl diisocyanate (H₁₂MDI) and isophorone diisocyanate (IPDI)). The pressure had no effect on hydrolysis in the range of 70-250 bar. The decomposition temperature of the PU samples increased in the following order: TDI-PU (199℃) < H₁₂MDI ≈ IPDI ≈ HDI (218-220℃) < MDI-PU (237℃). This order of increase in temperature is related to the electron-donating ability of the group to connected to the nitrogen of the urethane unit. When the temperature of the (PU + water) mixture reached the specific decomposition temperature, the PU samples hydrolyzed completely within 5 min into primary amine and 1,4-butanediol. The hydrolysis products from MDI-PU and H₁₂MDI-PU were separated into a liquid phase rich in (BD + water) and a solid low phase rich in amine, whereas the products from TDI-, IPDI-, and HDI-PU existed in a single aqueous phase.