• KSBB Journal
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• 제28권6호
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• pp.400-407
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• 2013

The growth characteristics and production of color pigments by Monascus strains were investigated during liquid culture, and production of monacolin K in red mold rice was carried out by solid state fermentation. Four different Monascus ruber strains were cultured in potato dextrose yeast extract broth (PDYB) media at $25^{\circ}C$ for 15 days. The high producing strain for red pigment was not corresponded to the strain for yellow pigment. Production of red pigment was high in the strain causing the fast pH change in culture broth. Production of monacolin K in red mold rice by solid state fermentation was influenced by a combination of wet cell weight and spore density in inoculum by liquid culture. Most strains showed the high production of monacolin K in red mold rice, when submerged fermentation was carried out for 5 days as inoculum for solid state fermentation. These results suggest that submerged fermentation period of inoculum have an effect on the production of monacolin K in red mold rice by solid state fermentation, and monacolin K in red mold rice could be increased by controlling the condition of submerged fermentation for inoculum.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2001년도 추계학술발표대회
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• pp.355-358
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• 2001

팽나무버섯균의 배양환경조사에서 최적배양온도는 $20^{\circ}C$, pH는 $6.0{\sim}7.5$ 탄소원은 yellow sugar. potato starch. 질소원은 soybean flour. Yeast extract. 무기염류는 $KH_2PO_4,\;MgSO_4,\;7H_2O$를 0.05%(w/v)에서 우수한 생장을 나타냈다. 액체종균 배양 실험에서는 전 배양시 erlenmeyer flask에서 6 일간 $21^{\circ}C$, 125rpm으로 배양 후 본 배양시 5% 접종량, 1.0vvm 통기로 4-5 일 배양하였을 때 균체량이 높게 나타났다.

• Mycobiology
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• 제34권4호
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• pp.196-199
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• 2006

Effects of various preservation periods and subcultures on fruiting body formation of Cordyceps militaris were investigated using EFCC C-10995 single ascospore strains. Fruiting body formation by original strains was profuse when preserved at $4^{\circ}C$ for $5{\sim}6$ months. Fruiting from subcultures was stable till second to sixth subcultures, after which it decreased sharply. The more the colony color of subcultures changed, the less the fruiting bodies formed. Liquid inoculum preparation of single ascospore strains in the same or separate broths did not affect fruiting body formation. Similarly, two strains C-10995-3 and C-10995-6 in different numbers during liquid inoculum preparation produced similar fruiting bodies.

• 한국균학회지
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• 제31권2호
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• pp.94-97
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• 2003

버들송이의 액체종균 제조를 위한 배양조건과 종균으로 사용시의 접종량을 검토하기 위한 시험 결과 배지 종류로는 감자추출배지(PSB)가 가장 좋았으며 온도는 $25^{\circ}C$, pH는 7.0에서 좋았다. 접종량별로는 $10{\sim}15ml$ 접종한 것이 우수하였다. 버들송이의 액체종균은 13l들이의 간이배양 장치를 제작하여 감자추출배지 10l에 10일간 배양후 균체건조량 21.8 g을 얻었다. 이를 병재배용 850 ml PP병의 톱밥배지에 10m1(균체량 21.8mg상당)씩 접종한 결과 배지 배양기간은 30일로써 톱밥종균을 사용할 때 보다 5일이 단축되었다. 또한 버섯발생병은 톱밥종균을 사용할 때의 75%에 비하여 92%로 높아졌고 자실체 수량도 122g으로 톱밥종균보다 10% 증수되었다. 따라서 본 액체종균 배양기술은 버섯 병재배 시설을 활용한 버들송이의 연중 재배에 도입하므로써 안정된 버섯생산으로 농가소득 증대에 기여할 것으로 본다.

• 한국버섯학회지
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• 제19권2호
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• pp.109-113
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• 2021

현재 양송이 종균은 1980년대 개발된 방법으로 제조되고 있어, 양송이 종균 품질 향상을 위한 새로운 제조 방법을 개발하였다. 그 결과 균사체량이 5.92±0.52 g/L로 가장 많이 배양된 CDB(compost dextrose broth) 배지에서 24℃에서 120 rpm으로 진탕 배양(24시간/일) 하는 통기식(2.5 L/min) 액체배양법으로 배양기간 10일이 액체 접종원 사용으로 양호했으며, 양송이 밀 배지 종균 생산은 밀 배지 50 g당 액체 접종원 1ml을 적정 접종량으로 하여 10일 배양하면 종균의 뭉침과 흔드는 작업없이 적절하게 배양되었음을 보고한다.

• Mycobiology
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• 제38권3호
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• pp.189-194
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• 2010

This study investigated the cultural characteristics of Shimizuomyces paradoxus in different nutritional and environmental conditions. The highest mycelial growth was observed in Schizophyllum (mushroom) genetics complete medium plus yeast extract agar medium, and the optimal temperature and pH were $25^{\circ}C$ and pH 8.0, respectively. The optimal carbon and nitrogen sources were 1% dextrose and 1% peptone in agar. However, in liquid culture the highest dry mycelium weight was found for the potato dextrose agar and potato sucrose agar broths. The optimum inoculum size was five mycelial discs (5 mm) per 100 mL of broth, and the optimum liquid culture period was 25 days. This is the first ever report of S. paradoxus cultural characteristics.

• Mycobiology
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• 제35권2호
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• pp.54-61
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• 2007

Two isolates of Tricholoma matsutake T-008 and T-034, preserved in Entomopathogenic Fungal Culture Collection (EFCC) of Korea, were used in the present study. The isolates had 100% Bootstrap homology with Tricholoma matsutake U62964 and T. matsutake AB188557 and AF309538 preserved in Gene Bank of NCBI. Mycelial growth of T. matsutake was highest in TMM and MYA at $25^{\circ}C$. The highest dry wt. of mycelium was obtained after 65 days of culture, when 6 mycelial discs were inoculated in 100 ml of broth in 250 ml shaking flask. Mycelial mats were observed in clumped condition at the inoculation sites of pine forest after two weeks of inoculation. After 5 months of inoculation, mycelia mats were observed growing inside soil and walls of a few inoculation sites, while mycelial mats growth up to $5{\sim}8$ cm were observed in the roots of pine tree after 6 months. The survival rate of the inoculum was about 40% of the total inoculation sites. The survival rate was found below 20% when the mycelium was inoculated in the summer. The reasons for low survival rates of the mycelium were mainly due to dry season and the soil-borne small animals such as earthworm and mole. After one year of inoculation, no external difference was observed between the artificially inoculated mycelia and the naturally existing mycelia of T. matsutake. The present study showed that fruiting bodies of T. matsutake could be produced by artificial inoculation under the appropriate environmental conditions.

• 한국균학회지
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• 제32권2호
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• pp.95-100
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• 2004

목질진흙버섯의 균사생육에 적합한 배지로는 MYA(malt yeast agar)와 SMS(soybean flour malt extract sugar)이며, MYA를 기본배지로 사용하였다. 균사생육은 $30^{\circ}C$의 온도와 pH는 5.0에서 생장이 양호하였다. 영양원으로는 malt extract 2%, yeast extract 0.2%와 $KH_{2}PO_{4}$ 0.1% 상태에서 균사생장 및 밀도가 양호하였다. C/N비는 10 : 1의 비율이 가장 적합하였다. 삼각플라스크배양을 위해서는 용기부피 300 ml Shake flask에 100 ml의 배양액을 넣고 $5{\sim}6$개 균사체 절편을 접종하여 20일간 배양하면 높은 고립상의 균체를 양호하게 증식시킬 수 있었다. 대량 액체종균 배양을 위해 접종원을 균질기로 분쇄하여 통기량 2.0 vvm의 조건에서 12일 배양하면 높은 균사의 생장을 보였고 접종에도 수월하였다.

• 한국균학회지
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• 제27권1호통권88호
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• pp.1-9
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• 1999

느타리버섯 8품종의 균사생장은 $25{\sim}30^{\circ}C$의 온도 범위와 $5.5{\sim}6.5$의 pH 범위에서 가장 우수하였다. 영양원의 선발시험에서 탄소원으로서는 황백당, 밀가루 및 옥수수가루가, 그리고 질소원으로서는 대두분이 공시균주의 균사생장이 가장 우수하였다. 농업적으로 이용성이 우수한 황백당 3%, 대두분 0.3%, 인산칼륨 0.05%, 황산마그네슘 0.05%를 배지 조성으로 하는 농업용 액체배지를 선발하였다. 삼각플라스크 배양에서는 250 ml의 erlenmeyer flask에서 보다는 250ml의 shake flask에서 직경 6 mm의 균사절편을 2개 접종하여 배양액량 100ml에서 배양했을 때 공시균주의 균사체 생산이 우수하였다. 그리고 erlenmeyer flask에서 배양방법을 달리하여 느타리버섯의 균사를 배양하였을 때, $45^{\circ}$ 경사배양한 처리구에서 균사체 생산이 가장 우수하였으며, 길이 $50mm{\times}$ 직경 10mm의 유리막대를 넣고 배양한 처리구에서는 균체량이 가장 적었지만 펄프형의 균사생육을 유도할 수 있었다.

• 산업기술연구
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• 제21권A호
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• pp.343-349
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• 2001

This study characterizes the growth of white rot fungi Phanerochaete chrysosporium IFO 31249) and lignin peroxidase(LiP) activity in different submerged culture media. P. chrysosporium was grown in the form of pellet of various sizes from a spore inoculum under shaking liquid culture condition. While the growth of mycelia was higher under the nitrogen-sufficient culture than under the nitrogen-limited culture, ligninase activity was relatively lower. The lignin peroxidase appeared in nitrogen-limited culture and was suppressed by excess nitrogen. High level(40U/l) of lignin peroxidase activity was obtained in the growth medium containing 1.5mM veratryl alcohol, a secondary metabolite of P. chrysosporium. Lignin peroxidase production was not observed under conditions of nitrogen sufficiency or in balanced media, suggesting that control parameters could increase the activity by manipulating the secondary metabolism.