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http://dx.doi.org/10.4489/MYCO.2006.34.4.196

Effect of Preservation Periods and Subcultures on Fruiting Body Formation of Cordyceps militaris In Vitro  

Sung, Jae-Mo (Department of Applied Biology and Entomopathogenic Fungal Culture Collection (EFCC), Kangwon National University)
Park, Young-Jin (Department of Applied Biology and Entomopathogenic Fungal Culture Collection (EFCC), Kangwon National University)
Lee, Je-O (Department of Applied Biology and Entomopathogenic Fungal Culture Collection (EFCC), Kangwon National University)
Han, Sang-Kuk (Department of Applied Biology and Entomopathogenic Fungal Culture Collection (EFCC), Kangwon National University)
Lee, Won-Ho (Department of Applied Biology and Entomopathogenic Fungal Culture Collection (EFCC), Kangwon National University)
Choi, Sung-Keun (Department of Applied Biology and Entomopathogenic Fungal Culture Collection (EFCC), Kangwon National University)
Shrestha, Bhushan (Department of Applied Biology and Entomopathogenic Fungal Culture Collection (EFCC), Kangwon National University)
Publication Information
Mycobiology / v.34, no.4, 2006 , pp. 196-199 More about this Journal
Abstract
Effects of various preservation periods and subcultures on fruiting body formation of Cordyceps militaris were investigated using EFCC C-10995 single ascospore strains. Fruiting body formation by original strains was profuse when preserved at $4^{\circ}C$ for $5{\sim}6$ months. Fruiting from subcultures was stable till second to sixth subcultures, after which it decreased sharply. The more the colony color of subcultures changed, the less the fruiting bodies formed. Liquid inoculum preparation of single ascospore strains in the same or separate broths did not affect fruiting body formation. Similarly, two strains C-10995-3 and C-10995-6 in different numbers during liquid inoculum preparation produced similar fruiting bodies.
Keywords
Cordyceps militaris; Fruiting body production; Original strains; Single ascospore strains; Subcultures;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
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1 Smith, D. and Onions, A. H. S. 1994. The preservation and maintenance of living fungi. CAB International, Wallingford
2 Kornerup, A. and Wanscher, J. H. 1978. Methuen handbook of colour. 3rd ed. Eyre Methuen, London
3 Shrestha, B., Kim, H. K., Sung, G H., Spatafora, J. W. and Sung, J. M. 2004. Bipolar heterothallism, a principal mating system of Cordyceps militaris in vitro. Biotech. Bioprocess Engin. 9: 440-446   DOI   ScienceOn
4 Sung, J. M., Choi, Y. S., Shrestha, B. and Park, Y. J. 2002. Investigation on artificial fiuiting of Cordyceps militaris. Kor. J. Mycol. 30: 6-10   DOI
5 Yokoyama, E., Yamagishi, K. and Hara, A. 2004. Development of a PCR-based mating type assay for Clavicipitaceae. FEMS Microbiol. Lett. 237: 205-212
6 Yokoyama, E., Yamagishi, K. and Hara, A. 2005. Heterothallism in Cordyceps takaomontana. FEMS Microbiol. Lett. 250: 145-150   DOI   ScienceOn
7 Sung, J. M., Park, Y. J., Lee, J. O., Han, S. K., Lee, W. H., Choi, S. K. and Shrestha, B. 2006. Selection of superior strains of Cordyceps militaris with enhanced fiuiting body productivity. Kor. J. Mycol. 34: 131-137   과학기술학회마을   DOI
8 Yokoyama, E., Yamagishi, K. and Hara, A. 2003. Structures of the mating-type loci of Cordyceps takaomontana. Appl. Environ. Microbiol. 69: 5019-502   DOI
9 Sung, J. M. 1996. Insect-Borne Fungi of Korea. Kyo-Hak Publishing Co. Ltd., Seoul
10 Seaver, F. J. 1911. The Hypocreales of North America-IV. Mycologia 3: 207-230   DOI