• Title/Summary/Keyword: Ligusticum

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Embryological Characteristics on Seed Sterility of Ligusticum chuanxiong Hoit. (천궁의 종자불임에 관한 발생학적 특성)

  • Park, Chung-Heon;Lee, Man-Sang;Namkoong, Seung-Bak;Yu, Hong-Seob;Park, Hee-Woon
    • Korean Journal of Medicinal Crop Science
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    • v.12 no.3
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    • pp.209-213
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    • 2004
  • Ligusticum chuanxiong is receiving much attentions as one of the important medicinal crops with the increasement of the crude drug demands. This study was conducted to obtain the basic informations of breeding of Ligusticum chuanxiong. Embryological characteristics were examined to elucidate the process of male and female gametophytic development and fertilization. Meiosis and nucleus division of megaspore and microspore were proceeded normally. With regard to the formation of female gametophyte, only a half of female gametophyte developed to normal egg apparatus. While another 50% showed abnormal egg apparatus with poly-nuclei or non-nuclei ovule. The pollens developed from the micros pore were formed more than 90 % of normal pollen. It was difficult to observe fertilization because ovule tissue was very compact and cell was extremely tiny, but could be easely observed proembryo and embryo formation. Only 30 percent developed into proembryo and subsequently into embryo, and the others were degenerated.

Mass Propagation by Stem Cutting in Ligusticum chuangxiong Hort. (토천궁(土川芎)의 줄기삽목에 의한 대양번식(大量繁殖))

  • Yu, Hong-Seob;Bang, Jin-Ki;Kim, Young-Guk;Lee, Bong-Ho
    • Korean Journal of Medicinal Crop Science
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    • v.7 no.3
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    • pp.200-204
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    • 1999
  • Ligusticum chuanxiong has been an important oriental crude drug and cultivated for a long time in Korea. Ligusticum chuanxiong has been vegetatively propagating crop without seed due to its sterility and would be an allodiploid derived from the hybridization of two allied species. Generally, vegetative propagation is more costly per unit propagule than sexual(seedling) propagation. This study was conducted to find out the techniques of mass propagation by stem cutting for reduced seedling cost. When cutted on August 30 with 1st to 2nd internode, its highest rooting percentage was 81 percent. Rhizome yield of plants cutted in 50 percent on September 1 decreased to 9 percent less than control (rhizome propagation). Rooting percentage of rooted cutting plants after field planting on October 25 was the highest being 87 percent. Rhizome yield with planting appeared 127 and 120kg/10a on September 25 and October 25, respectively.

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Effect of Ligusticum chuonxiong Hort Extracts on the Bioactivity in High-fat diet-fed Obese Rats (천궁 추출물이 고지방식이로 유도된 비만흰쥐의 생체활성에 미치는 영향)

  • Heo, Ye-Young;Ha, Bae-Jin
    • Journal of Food Hygiene and Safety
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    • v.26 no.4
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    • pp.370-376
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    • 2011
  • This study was performed to investigate the antioxidative effect of Ligusticum chuanxiong Hort extracts (LCE) against the hyperlipidemia of high-fat diet-fed obese rats. The rats were divided into the three groups (normal group, control group and sample group) to perform the experimental research. 1.5 ml/kg of LCE was intraperitoneally administered into the sample group for 21 days. The equal dose of 0.9% saline was intraperitoneally administered into the normal group and the control group. On day 22, they were anesthetized with ether and dissected. The levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT) were examined in serum of rats. Superoxide dismutase (SOD) was measured in mitochondrial fraction. Malondialdehyde (MDA), catalase (CAT), and glutamate peroxidase (GPx) were determined in liver homogenate. High-fat diet markedly increased the levels of AST, ALT and MDA, significantly decreasing those of SOD, CAT and GPx. But Ligusticum chuanxiong Hort-pretreatment decreased the levels of AST, ALT, and MDA. increasing those of SOD, CAT and GPx. These results demonstrated the antioxidative effects, suggesting that LCE could be the candidate for the functional material.

Effects of the Essential Oil Components from Ligusticum chuanxiong on Proinflammatory Mediators of RAW264.7 Macrophage Cells

  • Lim, Hye-Rim;Shin, Seung-Won
    • Natural Product Sciences
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    • v.16 no.4
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    • pp.259-264
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    • 2010
  • The essential oil fraction was obtained from the underground parts o of Ligusticum chuanxiong (Umbelliferae) by steam distillation, and its main components, Z-ligustilide and butylidene phthalide, were isolated by column chromatography. Its essential oil fraction and the isolated main components were examined for effects on their anti-inflammatory properties in RAW 264.7 macrophage cells to develop a new natural anti-inflammatory drug. The results showed that the L. chuanxiong essential oil fraction and its main components, Z-ligustilide and butylidene phthalide, inhibited the production of nitric oxide significantly in lipopolysaccharide (LPS)-treated RAW 264.7 cells. LPS-induced interleukin-$1{\beta}$ (IL-$1{\beta}$), interleukin-6 (IL-6), and tumor necrosis factor-alpha (TNF-$\alpha$) production was also decreased in a dose-dependent manner. In addition, western blot analysis revealed that the L. chuanxiong essential oil fraction and also its main components, Z-ligustilide, and butylidene phthalide reduced the expression levels of cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS).

Two Unrecorded Enophytic Fungi Isolated from Root of Ligusticum chuanxiong in Korea: Pithomyces chartarum and Plectosphaerella niemeijerarum (토천궁의 뿌리에서 분리된 2종의 국내 미기록 내생균: Pithomyces chartarum and Plectosphaerella niemeijerarum)

  • Park, Hyeok;Jung, Chung Ryul;Eom, Ahn-Heum
    • The Korean Journal of Mycology
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    • v.47 no.4
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    • pp.329-335
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    • 2019
  • Endophytic fungal strains were isolated from the sterilized roots of a medicinal plant, Ligusticum chuanxiong and identified based on morphological characteristics and molecular analysis of internal transcribed spacer, large subunit rDNA, and beta-tubulin DNA regions. Our results confirmed the presence of Pithomyces chartarum and Plectosphaerella niemeijerarum in the fungal strains. To the best of our knowledge, the fungal strains have not been reported in Korea. In this report, we describe morphological characteristics and phylogenetic trees of these endophytic fungal strains.

Study on Cytotoxic Activities of the Essential Oil Compounds from Ligusticum chuanxiong against Some Human Cancer Strains (토천궁 정유 성분의 수종 사람 암 세포주에 대한 세포 독성)

  • Sim, Youn;Shin, Seung-Won
    • YAKHAK HOEJI
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    • v.55 no.5
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    • pp.398-403
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    • 2011
  • Ligusticum chuanxiong (Umbelliferae) is a perennial herb that has been used for invigoration of blood in Korean traditional medicine. It is especially important in gynecological therapy of amenorrhea and dysmenorrhea. In this study, the essential oil of L. chuanxiong was obtained by steam distillation and its main components of L. chuanxiong, Z-ligustilide and butylidene phthalide, were isolated by silica gel column chromatography. We investigated the cytotoxic effects of the essential oil fraction of L. chuanxiong and its main components on MCF-7, HeLa and SK-Hep-1 cell lines by measuring the number of surviving cancer cells after treatment through direct cell counting and MTT analysis, and by examining the morphological changes under the microscope. The essential oil from the rhizomes of L. chuanxiong and its main components showed significant cytotoxic activities for all three tested cell lines. We also observed morphological changes of shrinking and blebbing in the membranes of the three cell lines, depending on the concentration of L. chaunxiong oil or its main components.

Molecular Marker to Identify and Origin of Cnidii Rhizoma from Korea and China (천궁의 기원과 식별을 위한 분자마커)

  • Song, Im-Geun;An, Bo-Ram;Seo, Bu-Il;Park, Seon-Joo
    • The Korea Journal of Herbology
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    • v.24 no.4
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    • pp.1-8
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    • 2009
  • Objectives : This study was carried out to discriminate origin and molecular marker of oriental medicine "Cnidii Rhizoma" be circulated between Korea and China, which is difficult to discriminate from morphological distinction because of a fragmental materials of roots. Methods : Materials were collected randomly from a medicinal herb markets in Korea and China and be analyzed with ITS (internal transcribed spacer) regions of nuclear ribosomal DNA (nrDNA). Results : As a results, ITS regions of nrDNA was shown to be identify as three molecular markers. "Cnidii Rhizoma" was made up syster group of the genus Ligusticum L. and divided into three groups with "Tou-chun-gung", "IL-chun-gung" and "China-chun-gung". Conclusions : From the analysis of ITS regions of nrDNA, we presumed that it is the same origin of "Cnidii Rhizoma" from Korea and China because of phylogenetic tree consisted of sister groups with the genus Ligusticum than the genus Cnidium.

Studies on the Umbelliferous Fruits (XI) -'Kobon' (Korean Gaoben)- (미나리과(科) 식물(植物)의 과실(果實)에 관한 연구(硏究)(XI) -고본에 대하여-)

  • Suk, Kuy-Duk
    • Korean Journal of Pharmacognosy
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    • v.6 no.3
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    • pp.131-135
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    • 1975
  • In continuation of studies on the Umbelliferous fruits the inner morphology of 'Kobon' fruit was examined, 'Kobon' is an original plant of Korean Gaoben, its scientific name being given Angelica tenuissima NAKAI or Ligusticum tenuissimum KITAGAWA. The arrangement of vascular bundles is the same as those of the genus Ligusticum or Tilingia and Conioselinum. The tracheids reach nearly the top of the ribs, and this is similar to that of the genus Conioselinum. From these characters and the results of studies on the inner morphology of the fruits of Umbelliferous plants related to 'Kobon,' the origin of 'Kobon' was identified as the genus Conioselinum.

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Disease Resistance and High Yielding Ligusticum chuanxiong Hort. Variety ‘Sinto’ (천궁 내병 다수성 신품종 ‘신토’)

  • Park, Chung-Heon;Yu, Hong-Seob;Park, Hee-Woon;Seong, Nak-Sul;Park, Chun-Geon;Kim, Young-Guk;Kim, Chung-Guk
    • Korean Journal of Medicinal Crop Science
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    • v.10 no.5
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    • pp.415-418
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    • 2002
  • Ligusticum chuanxiong Hort. is one of the most important root-using medicinal crops in Korea. A new variety 'Sinto' (Suwon 2) was developed from the population of Muju local through phenotyptic restricted mass selection at National Crop Experiment Station, RDA. in 2001. The average yield of this variety was about 2,260 kg/ha in RYT at three location from 1999 to 2001 which were about 20% higher than that of Pyeongchang local. Sinto showed a strong resistance to powdery mildew. This variety may be well adaptable to mountainous mid-southern areas in Korean peninsula.

Factors Influencing Rooting and Growth in Stem-Cut Planting of Ligusticum chuangxiong Hort (천궁(川芎)의 줄기 삽식시 발근(發根)과 생육(生育)에 영향(影響)하는 요인(要因))

  • Ohk, H.C.;Lee, H.S.;Chae, Y.A.
    • Korean Journal of Medicinal Crop Science
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    • v.5 no.2
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    • pp.119-125
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    • 1997
  • This study was carried out to know the factors affecting rooting and growth in stem cutting of Ligusticum chuangxiong for mass propagation. The results indicated that : (1) Rooting and growth were better in perlite which has higher air permeability. (2) Sub-irrigation with 15min long with 30min interval was more favorable for growth. (3) Proper tempeature for rooting and growth ranged from $25^{\circ}C\;to\;30^{\circ}C$ while photoperiod had no effect on rooting and growth.

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