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Effects of the Essential Oil Components from Ligusticum chuanxiong on Proinflammatory Mediators of RAW264.7 Macrophage Cells  

Lim, Hye-Rim (College of Pharmacy, Duksung Women's University)
Shin, Seung-Won (College of Pharmacy, Duksung Women's University)
Publication Information
Natural Product Sciences / v.16, no.4, 2010 , pp. 259-264 More about this Journal
Abstract
The essential oil fraction was obtained from the underground parts o of Ligusticum chuanxiong (Umbelliferae) by steam distillation, and its main components, Z-ligustilide and butylidene phthalide, were isolated by column chromatography. Its essential oil fraction and the isolated main components were examined for effects on their anti-inflammatory properties in RAW 264.7 macrophage cells to develop a new natural anti-inflammatory drug. The results showed that the L. chuanxiong essential oil fraction and its main components, Z-ligustilide and butylidene phthalide, inhibited the production of nitric oxide significantly in lipopolysaccharide (LPS)-treated RAW 264.7 cells. LPS-induced interleukin-$1{\beta}$ (IL-$1{\beta}$), interleukin-6 (IL-6), and tumor necrosis factor-alpha (TNF-$\alpha$) production was also decreased in a dose-dependent manner. In addition, western blot analysis revealed that the L. chuanxiong essential oil fraction and also its main components, Z-ligustilide, and butylidene phthalide reduced the expression levels of cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS).
Keywords
Ligusticum chuanxiong; essential oil; ligustilide; butylidene phthalide; anti-inflammatory effect; nitric oxide; IL-$1{\beta}$; IL-6; TNF-$\alpha$; COX-2; iNOS;
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