• Journal of Life Science
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• v.14 no.2
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• pp.325-330
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• 2004

The RAD3 gene of Saccharomyces cerevisiae is required for excision repair and is essential for cell viability. RAD3 encoded protein possesses a single stranded DNA-dependent ATPase and DNA and DNA-RNA helicase activities. To examine the extent of conservation of structure and function of RAD3 during eukaryotic evolution, the RAD3 homolog gene was isolated by screening of genomic DNA library. The isolated gene was designated as HRD3 (Homologue of RAD3 gene). The over-expressed HRD3 protein was estimated to be a 75 kDa in size which is in good agreement with the estimated by the nucleotide sequence of the cloned gene. Two-dimensional gel electrophoresis showed that a number of other protein spots dramatically disappeared when the HRD3 protein was overexpressed. The overexpressed RAD3 protein showed a toxicity in E. coli host, suggesting that this protein may be involved in the inhibition of protein synthesis and/or degradation of host protein. To determine which part of HRD3 gene contributes to the toxicity in E. coli, various fusion plasmids containing a partial sequence of HRD3 and lac'Z gene were constructed. These results suggest that the C-terminal domain of HRD3 protein may be important for both toxic effect in E. coli and for its role in DNA repair in S. pombe.

• Journal of Life Science
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• v.20 no.11
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• pp.1648-1655
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• 2010

The flavor extracts of Mideoduck muscle and its juices were concentrated by simultaneous distillation and extraction (SDE) and solid-phase microextraction (SPME) methods. Each component present in the extracts was identified with GC and GC-MS by the n-paraffin hydrocarbon retention index and standard MS library data system. By SDE, $371.3\;{\mu}g/g$ of hexanal, $80.1\;{\mu}g/g$ of 1-tridecanol, $72.1\;{\mu}g/g$ of (Z)-4,5-dimethylhex-2-en-4-ol with other alcohols, aldehydes and acids were present in the flavor extracts, with the alcohols having the highest composition and being the most important factor in Mideoduck muscle flavor. By SPME, 9 alcohols, 1 acid, 1 aldehyde, 1 hydrocarbon, 1 ester, 1 amine and 2 ketones were detected in the extracts, with alcohol such as 1-nonanol, 1-decanol and 1-tridecanol as the major components. In SPME, the muscle sample, consisting of $31.6\;{\mu}g/g$ of 1-nonanol, $20.3\;{\mu}g/g$ of (E)-2-butenedioic acid dibutylester, and $26.7\;{\mu}g/g$ of heptadecanoic acid made up the 62.1% of total flavor extracts of Mideoduck muscle. The results of the SPME methods were similar to the composition of the raw material flavor of the sample even at a low concentration.

• Journal of Life Science
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• v.23 no.1
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• pp.1-7
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• 2013

Understanding salt tolerance mechanisms is important for the increase of crop yields, and so, several screening approaches were developed to identify plant genes which are involved in salt tolerance of plants. Here, we transformed the Arabidopsis cDNA library into a salt-sensitive calcineurin (CaN)-deficient ($cnb{\Delta}$) yeast mutant and isolated the colonies which can suppress salt-sensitive phenotype of $cnb{\Delta}$ mutant. Through this functional complementation screen, a total of 34 colonies functionally suppressed the salt-sensitive phenotype of $cnb{\Delta}$ yeast cells, and sequencing analysis revealed that these are 9 genes, including CaS, AtSUMO1 and AtHB-12. Among these genes, the ectopic expression of CaS gene increased salt tolerance in yeast, and CaS transcript was up-regulated under high salinity conditions. CaS-antisense transgenic plants showed reduced root elongation under 100 mM NaCl treatment compared to the wild type plant, which survived under 150 mM NaCl treatment, whereas CaS-antisense transgenic plant leaves turned yellow under 150 mM NaCl treatment. These results indicate that the expression of CaS gene is important for stress tolerance in yeast and plants.

• Journal of Life Science
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• v.30 no.7
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• pp.625-629
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• 2020

The Ocean is a rich source of diverse living organisms include viruses. In this study, we examined the microbial communities in the sea adjacent to Jeju Island in two seasons by metatranscriptomics. We collected and extracted total RNA, and, using the next-generation sequencing HiSeq 2000 and de novo transcriptome assembly, we identified 652,984 and 163,759 transcripts from the March and December samples, respectively. The most abundant organisms in March were bacteria, while eukaryotes were dominant in the December sample. The bacterial communities differed between the two samples, suggesting seasonal change. To identify the viruses, we searched the transcripts against a viral reference database using MegaBLAST with the most identified being bacteriophages infecting the marine bacteria. However, we also revealed an abundance of transcripts associated with diverse herpesviruses in the two transcriptomes, indicating the presence or possible threat of infection of fish in the sea around Jeju Island. This data is valuable for the study of marine microbial communities and for identifying possible viral pathogens.

• Journal of the Institute of Convergence Signal Processing
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• v.11 no.2
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• pp.177-182
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• 2010

The flow of a universal system-level design methodology consists of system specification, system-level hardware/software partitioning, co-design, co-verification using virtual or physical prototype, and system integration. In the developing process of a hardware component in system, the design phase has been regarded as a phase consuming lots of time and cost. However, the verification phase in which functionality of the designed component is verified has recently been considered as a much important phase. In this paper, the implementation of a verification environment which is based on SystemC infrastructure and verifies the functionality of a hardware component is described. The proposed verification system uses SystemC user-defined channel as communication interface between variables of SystemC module and registers of Verilog module. The functional verification of an UART is performed on the proposed verification system. SystemC provides class library for hardware modeling and has an advantage of being able to design a system consisting hardware and software in higher abstraction level than register transfer level. Source codes of SystemC modules are reusable with a minor adaptation on verifying functionality of another hardware component.

• Journal of Information Management
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• v.34 no.2
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• pp.67-88
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• 2003

There have been increased interests in information visualization. Information visualization has been considered as a way to summarize textual data so that the users can access large amount of data more efficiently and effectively. However, many information visualization techniques stem from scientific visualization techniques, which might be difficult for the regular users to understand. More importantly, the system models used by most of the information visualization techniques do not have real world counterpart. For example, most of the users do not represent or process the textual data in terms of fisheye view or a topological map. This means that there is no affordance on the current information visualization systems from the users point of view. In this paper, we analyzed this problem by using the user interface design principles to point out what lacks in the current information visualization systems. More specifically, we have applied Nielson's Heuristic Evaluation technique to review four representative information visualization techniques. The analysis results confirmed our original hypothesis on why the current information visualization systems are not part of the mainstream information systems. Finally, we suggested to invest more efforts in improving the currently prevalent and familiar bullet list type textual information presentation method based on the usability studies and the intelligent content analysis.

• Journal of Information Management
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• v.42 no.3
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• pp.71-94
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• 2011

Query by visual example is the principal query paradigm for expressing queries in a content-based image retrieval environment. Query by image and query by sketch have long been purported as being viable methods of query formulation yet there is little empirical evidence to support their efficacy in facilitating query formulation. The ability of the searcher to express their information problem to an information retrieval system is fundamental to the retrieval process. The aim of this research was to investigate the query by image and query by sketch methods in supporting a range of information problems through a usability experiment in order to contribute to the gap in knowledge regarding the relationship between searchers' information problems and the query methods required to support efficient and effective visual query formulation. The results of the experiment suggest that query by image is a viable approach to visual query formulation. In contrast, the results strongly suggest that there is a significant mismatch between the searchers information problems and the expressive power of the query by sketch paradigm in supporting visual query formulation. The results of a usability experiment focusing on efficiency (time), effectiveness (errors) and user satisfaction show that there was a significant difference, p<0.001, between the two query methods on all three measures: time (Z=-3.597, p<0.001), errors (Z=-3.317, p<0.001), and satisfaction (Z=-10.223, p<0.001). The results also show that there was a significant difference in participants perceived usefulness of the query tools Z=-4.672, p<0.001.

• Korean Journal of Food Science and Technology
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• v.26 no.2
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• pp.162-166
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• 1994

A rapid gas chromatographic (GC) profiling method for the simultaneous analysis of volatile and nonvolatile organic acids was applied to alcoholic beverages (white wine, red wine, brandy, and beer). It involves the solid-phase extraction of organic acids using Chromosorb P as the sorbent and diethyl ether as the eluent with subsequent triethylamine treatment. The resulting triethylammonium salts of acids were directly converted to volatile tert.-butyldimethylsilyl derivatives, which were analyzed by dual-capillary column GC and GC-mass spectrometry. From the alcoholic beverages studied, more than 29 organic acids were detected. When the simplified retention infer (RI) spectra of organic acids, and the direct comparisor method between alcoholic beverages and a test sample were attempted to identify a test sample, it was quickly recognized to be a red wine with the 998 ppt match quality value.

• Journal of Korea Society of Industrial Information Systems
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• v.24 no.6
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• pp.43-53
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• 2019

In recent years, there have been many cases of damage to indoor air hazardous materials, and major damage due to the lack of quick action. In this regard, the system is intended to establish for sending push messages to the user's mobile when the concentration of hazardous substances is exceeded. This system extracts data with IoT system such as Arduino and Raspberry Pi and then constructs database through MongoDB and MySQL in cloud computing system. The database is imported through the application server using NodeJS and sent to the application for visualization. Also, when receiving signals about a dangerous situation in IoT system, push message is sent using Google FCM library. Mobile application is developed using Android Web view, and page to enter Web view is developed using HTML5 (HTML, Javascript CSS). The application of this system enables real-time monitoring of indoor air-dangerous substances. In addition, real-time information on indoor/outdoor detection location and concentration can be sent to the user's mobile in case of a risk situation, which can be expected to help the user respond quickly.

• The Journal of Korean Academy of Sensory Integration
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• v.11 no.2
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• pp.41-56
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• 2013

Objective : The purpose of this study tried to present the performed average and range through systematic review about assessment tools that measures the praxis ability of sensory integrative function for foreign and domestic normal children. Methods: With making use of Medline, PubMed, Ovid, Eric, the Library of Congress, National Science and Technology Information Center, Kiss, RISS, and the Google search engine, we searched the name of assessment tool on the basis of the framework of the theory of sensory integration. Target paper was thesis and Journal published from January 2000 to April 2011. Results: Total of 24 papers were retrieved, used assessment tools were Bruininks-Oseretsky Test of Motor Proficiency (BOTMP), Bruininks-Oseretsky Test of Motor Proficiency, Short Form (BOTMP-SF), Bruininks-Oseretsky Test of Motor Proficiency, Second Edition (BOT-2), Bruininks-Oseretsky Test of Motor Proficiency, Second Edition, Short Form (BOT-2-SF), Clinical Observations of Motor and Postural Skills (COMPS), Motor Assessment Battery for Children (MABC), Clinical Test of Sensory Interaction with Balance (CTSIB), and subtest of Sensory Integration and Praxis Test (SIPT). We presented the average and range of assessment tools applied to normal children in each country. Conclusion: A performed result in other cultural and domestic normal children which this study presented will provide a standard in comparison with performance of children with disabilities who are the actual clinical evaluation.