• Natural Product Sciences
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• 제13권2호
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• pp.123-127
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• 2007

We investigated effect of small black soybean fraction (SBSF) T cell-mediated responses for tumor surveillance and proliferation in leukemia cells in vitro. Each SBSF butanol fraction (SBSFBu) and SBSF chloroform fraction (SBSFCh) was administered p.o. once a day far 21 days in BALB/c mice and then levels of serum cytokines and subpopulation of lymphocytes were measured. Moreover, SBSF fraction was treated into the cultured various cell lines for proliferation in leukemia cell lines, NO production by RAW264.7 cells, and expression of p53 gene in U937 leukemia cells. These results showed that SBSFBu increased levels of serum IL-4but not IL-2 and IFN-${\gamma}$, and increased expression of CD4$^+$ T cells and CD8$^+$ T cells in splenocytes in vivo, while SBSFCh increased levels of serum IL-2 and IFN-${\gamma}$ but decreased IL-4, and increased CD8$^+$ T cells but not CD4$^+$ T cells. Moreover, both of SBSFBu and SBSFCh inhibited proliferation of HL60, U937, and L1210 leukemia cell lines in a dose-dependent manner, up-regulated NO production by RAW264.7 cells in a dose-dependent manner, and enhanced expression of p53 gene in U937 leukemia cells. Our findings indicate that SBSFBu and SBSFCh may enhance T cell-dependent immune responses, and that both of SBSFBu and SBSFCh may inhibit proliferation of leukemia cells by up-regulation of NO production and expression of p53 gene.

• Archives of Pharmacal Research
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• 제16권3호
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• pp.219-226
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• 1993

For the analysis of structure relationship of ar-turmerone analogues, the compounds containing the various substituents on the phenyl ring and 1(or 2)-naphthyl group in the place of phenyl of ar-turmerone were prepared and tested their cytotoxicity against HL-60, K-562, and L1210 leukemia cells in vitro. The substituents at para position are methoxy, phenoxy, methyl, trifluoromethyl, fluoro, and chloro. At meta position methoxy, methyl, trifluoromethyl, or chloro groups at ortho position mathoxy or chloro group were introduced. Against HL-60 and K-562 cells, $ED_{50}$ values of the analogues are ranged from 0.8 to $30.0\;\mu{g/ml}$. Againste L1210 cell, these are located more than $20.0\;\mu{g/ml}$. However, 5-carbone-thoxy-2-methyl-6(1-naphthyl)-2-octen-4-one (5n)possesses $ED_{50}$ valuses 0.8, 2.1, $6.5\;\mu{g/ml}$ against HL-60, L1210 cells, respectively. The electronic nature of the substituents on phenyl ring of ar-tumerone dose not affect the biological activity. Therefore the flat structure of aromatic potion of ar-tumerone analogues is the more important factor for their activity rather than its electronic nature. The potentiation of the cytotoxicity with the enlargement of aromatic ring region also supports the importance of the plane structure of this area. The restriction of the single bond rotation between C-6 and aromatic ring through the introduction of substituents at the ortho position of phenyl ring and the increment of size of alkyl group at C-6 position enhances the activity. Therefore the effective conformation should by the one having the orthogonal arrangement between the aromatic ring and the side chain.

• Biomolecules & Therapeutics
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• 제7권4호
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• pp.371-376
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• 1999

By cytotoxicity screening of the 65 Korean medicinal plants against leukemia L1210 and P388D$_{1}$ cell line using the MTT assay in vitro, Albizzia julibrissin was studied. This plant was extracted with MeOH and MeOH extract was solvent-fractionated with CHCl$_{3}$, EtOAc and n-BuOH in sequence. Each fraction by various solvents system was purified by column chromatography and preparative TLC, and four compounds were isolated. The structure of each compound was deduced from UV, IR, $^{1}$H-HMR, $^{13}$ C-NMR and CI-MS spectral data. The cytotoxic activity ($IC_{50}$/_ of the compounds, quercetin-3-rhamnoside, 4',5,7-trihydroxyfla-van-3-glucoside, spinasterol-3-glucoside and acacic acid lactone, were evaluated as 5 $\mu\textrm{g}$/ml, 2$\mu\textrm{g}$/ml, 1 $\mu\textrm{g}$/ml against L1210 and as 9$\mu\textrm{g}$/ml, 10$\mu\textrm{g}$/ml, 1.5 $\mu\textrm{g}$/ml, 1.5 $\mu\textrm{g}$/ml and 0.9 $\mu\textrm{g}$/ml against P388D$_{1}$, respectively.

• 한국약용작물학회지
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• 제12권4호
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• pp.315-320
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• 2004

와송 (OJB)의 면역 및 항암 효과를 관찰한 결과, 와송은 생쥐의 비장 및 흉선세포의 생존율을 증가시켰으며, 임파구 아집단분석 결과 비장 T세포를 유의성있게 증가시켰는데 비장 T세포 중의 $T_H$세포 및 Tc세포가 모두 증가되었고, 혈청 중 ${\gamma}-interferon$의 생성을 현저하게 증가시켰다. 또한 와송은 L1210세포 및 U937세포 등의 백혈병세포의 아폽토시스를 촉진시키는 항암능력을 보유하고 있었다.

• 동의생리병리학회지
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• 제18권5호
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• pp.1337-1342
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• 2004

The purpose of this research was to investigate the immunoregulatory effect and the leukemia cell apoptosis of EtOH extract of OGAPI(OGP). The proliferation of cultured splenocytes, thymocytes and mesenteric lymph node cells were enhanced by the addition of OGP. Splenic and thymic T lymphocytes, especially TH and Tc cells were significantly increased in OGP-administered mice. OGP markedly increased the production of γ-interferon in mice serum and accelerated the phagocytic activity in peritoneal macrophages. OGP treatment enhanced the apoptosis of L1210 mouse leukemia and Jurkat, Molt4 human leukemia cells, and increased the expression of apoptosis-related ICE, c-myc, p53 gene in Jurkat cell. These results suggest that OGP have an immunoregulatory action and anti-cancer activity.

• 생약학회지
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• 제30권1호
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• pp.7-11
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• 1999

The present work was carried out to examine the effect of costunolide on the growth of several cells and characteristics of U-937 human leukemia-derived cell line. Costunolide produced a potent antitumor activity in vitro dependent on concentration against several tumor cells such as P-388, L-1210 leukemia and SNU-5 stomach cancer cells. However, it showed less cytotoxicity on normal cells such as Maccaccus rheus monkey kidney cells (MA-104) up to 200 ${\mu}M$ concentration. An effect of cell differentiation by costunolide was assessed by its ability to reduce nitroblue tetrazolium (NBT), and to induce phagocytosis of latex particles. In order to establish whether costunolide induces U-937 cells to differentiate toward macrophage or granulocyte, esterase activities was measured. Based on these results, we found that costunolide having cytotoxicity on U-937 human leukemia cells was explained through differentiation inducing activity.

• Archives of Pharmacal Research
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• 제18권6호
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• pp.396-401
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• 1995

As a part of trials to develop the antitumor agent from tannins isolated from plants, the antitumor activity of peduculagin, an ellagitannin, isolated from Alnus hirsuta var. microphylla was examined in vitro and in vivo. In vitro, the cytotoxicity was determined by 0.4% typanblue dye exclusion method. peduculagin showed the dose-dependent cytotoxicity against human chronic myelogenous leukemia (K-562), human promyelocytic leukemia (HL-60), mouse lymphoid neoplasm (P388), mouse lymphocytic leukemia (L1210) and mouse sarcoma 180(S180) cell lines. $ED_{50}\; values\; (ED_{50})$ of each cell line were 5.30, 0.92, 2.78, 9.35 and $1.38 \mug/ml$ respectively. The most sensitive cell line was HL-60. In vivo, pedunculagin was administered to ICR mouse with the doses of 50 and $100{\;}{\mu}g/ml$intraperitoneally once at 20 days before S180 inoculation. peduculagin showed the antitumor activity and its T/C ratio (%) was 120.82% in the group of both concentrations.

• 혜화의학회지
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• 제10권2호
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• pp.73-81
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• 2002

In the literal study of anti-tumor effects of Jakeumjung. the results were as follows. 1. Jakeumjung is a traditional oriental medical prescription which is composed of Cremastare appeniculatae tuber, Euphorbiae pekinensis radix, Toosendan fructus, Chinensis galla, Moschus, Realgar and Cinnabaris. 2. Jakeumjung is applied to patients by administering or external application. When it is administered for patients, pertinent dose is 0.6~1.5g twice or three times per one day. When it is applied by external application, we melt it by water or vinegar and apply it to patients. 3. Effects of Jakeumjung are expelling toxin and pestilence, counteracting pathogen and relieving stagnation, detumescence and stopping pain. So it is used for detoxification from ancient. In recent, it is often used for cancer such as breast cancer, pancreatic cancer, epigastric cancer, acute leukemia, lymphoma, thymus cancer and skin cancer. 4. From the various experiments, Jakeumjung has been proved to have antifungal and antitumor effects. It inhibits and kills L7212, L1210 cells of leukemia. Especially, it acts in S stage of cell period. 5. Jakeumjung includes mineral medicines such as Realgar, Cinnabaris. So if we execute progressive study for anticancer effects and safety, the boundary of oriental medicine of using mineral medicines for cancer therapy will magnify in the future.

• 한국식품영양학회지
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• 제15권2호
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• pp.151-157
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• 2002

대두 배아로부터 saponin을 분리.정제하여 유리기 억제효과 및 동물의 암세포에 대한 세포 독성을 조사하였다. 유리기 억제활성에 대한결과를 보면 대두 배아 saponin을 0.5 및 1.0%씩 첨가한 급여 식에서 4주 동안 사육한 후 간장을 절취하여 간장 획분의 활성산소 및 항산화 관련 효소에 대하여 평가하였다. 간장의 mitochondria 및 microtome 획분의 hydroxy radical(.OH)의 생성에 미치는 영향에서 급여식에 사포닌을 첨가한 급여군에서 .OH기의 생성억제 효과를 나타내었다. 간장의 microsome 획 분에서도 0.5 및 1.0% 투여군에서 $H_2O$$_2$생성억제 효과가 인정되었다. 또한 간장의 cytosol획분에서 $O_2$.$^{-}$의 생성은 대조군에 비하여 현저한 억제 효과를 나타내었다. 대두 배아 사포닌의 세포독성을 P338 (mouse Iyoupoid neoplasm)과 L1210 (mouse leukemia) 세포주를 사용하여 실시한 결과 200 $\mu$g/mL 농도에서 높은 성장억제효과를 나타내었다. 그리고 처리 농도에 따른 대두배아 추출물이 P338과 L1210에 대한 성장 효과 실험에서도 200 $\mu$g/mL에서 은 성장억제 효과가 나타났으며 농도가 높을 수록 완전히 억제되는 것을 볼 수 있었다.

• 한국식품과학회지
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• 제30권3호
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• pp.702-708
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• 1998

연구는 버섯을 이용한 기능성식품 개발 및 버섯가공 산업 육성에 기초자료를 제공하고자 표고버섯 및 느타리버섯 자실체, 균사체 및 폐상의 조단백다당체 분말을 0, 5, 25 mg/kg 농도로 생리식염수에 용해시켜 마우스(DBA/2와 ICR)에 주사하여 i) 백혈병$(L_{1210})$, 간암$(H_{22}),\;mouser{\;}sarcoma180\;(S_{180})$에 대한 항암효과, ii) 면역기전에 관련된 장기중량변화, 용혈반 형성 세포수 변화 등 조단백다당이 면역에 미치는 영향, 그리고 in vitro 세포독성실험을 수행하였다. 본 실험에서 사용한 모든 조단백다당류는 백혈병에 대해 항암효과를 보였는데 그 중 표고버섯자실체 25 mg/kg 처리구가 가장 높은 저지율(86%)을 보였다. 간암$(H_{22})$에 대한 항암효과는 표고버섯균사체 5 mg/kg 처리구를 제외하고 모든 처리구에서 저지효과를 보였는데, 그 중 느타리버섯 폐상 25 mg/kg 처리구가 가장 높은 저지율(87.6%)을 보였으며, 다음은 표고버섯자실체 25 mg/kg 처리구(71%)였다. Sarcoma180에 대한 항암효과는 표고버섯균사체와 자실체 25 mg/kg 처리구에서 각각 30.9,와 33.9%의 저지율을 보였다. In vitro 세포독성검사에서 각 시료의 최종농도 $50,\;100,\;200,\;400\;{\mu}g/{\mu}L$에서 $L_{1210}$에 대한 유의적 세포사망률은 보이지 않았다. 면역효과 실험에서 간장과 비장중량은 농도증가에 따라 증가하는 추세이나 현저한 차이는 없었다. 표고버섯균사체와 자실체 처리시 항체생성능력을 지닌 용혈반 형성 세포수는 대조군에 비해 현저히 높게 나타났다.