Browse > Article

Free Redical Scavenging and Cytotoxicity Activitives of Soybean Germ Saponin  

류병호 (경성대학교 의용공학부 식품공학과)
이홍수 (동부산대학 호텔외식조리과)
김현대 (동부산대학 호텔외식조리과)
Publication Information
The Korean Journal of Food And Nutrition / v.15, no.2, 2002 , pp. 151-157 More about this Journal
Abstract
This study was carried out to investigate functional activities of the free radical scavenging and germ of Glycin max. Merrill fur cytotoxicity toward P338 and L1210 cells derived from mouse. Effect of crude saponin were examined to oxygen radicals and their scavenger enzymes in liver fractions of Spragae-Dawley(SD) rats. Male rats were fed basic diets of control and experiment diets of 0.5∼1.0% crude saponin. There were no significant differences in hydroxy radical($.$OH) formation of liver mitochondria and microsomes in 1.0% group, while $.$OH formations were significantly decrease in 0.5% and 1.0% saponin compared with control group. Their oxygen radical(O$_2$$\^$$.$/) scavenging activities were significantly decrease in liver cytosol of 0.5% and 1.0% saponin group compared with control group. Soybean germ saponin was isolated purified by the method of HPLC to investigate the cytotoxicity of mouse cells by using the MTT assay. SA-1 saponin fraction of soybean germ showed to inhibit toward growth cell of P338 and L1210 cells and its showed less than 50% cytotoxicity These results suggest that the saponin may play a effective role in attenuating a oxygen radical formations and increasing a scavenger enzyme activities.
Keywords
soybean germ saponin; free radical scavenging; cytotoxicity;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Adlercreutz, H. : Phytoestrogens : Epidemiology and a possible role in cancer protection. Environ. Health Perspect., 103, 103-112(1995)   DOI
2 Aklecreutz, H., Hockerstedt, K., Bannwart, C., Bloigu, S., Hamalainen, E., Fotsis, T. and Ollus, A. : Effect of dietar y components, including lignans and phytoestrogens on sex hormone binding globulin. J. Steroid Biochem., 27. 1135-1144(1987)   DOI   ScienceOn
3 Messina, M. J., Persky, V., Setchell, K. D. R. and Barnes, S. : Soy intake and cancer risk, a review of in vitro and in vivo data, Nutr. and Cancer, 21, 113-124(1994)   DOI   ScienceOn
4 Tsukamoto, C., Kikuchi, A., Harada, K., Kitamura, K., Kudoh, S., Wasaki, T. and Okubo, K. : Group A acetyl saponin deficient mutant from the wild soybean. Phytochem., 31 (12), 4913-4142(1988)
5 大久保一良：吉城由美子, 吉城昌樹, 本知玄, 工藤重光：大豆配糖體成分の種類, 構造, 遺伝性, 生理活性:ニューフードインダストリー, 36, 17-27 (1994)
6 윤기도, 권동진, 홍석찬, 김수일, 정건섭 : 대두 및 대두발효식품의 항돌연변이성, 한국산업미생물학회지(1996)
7 Price, K. R., Johnson, I. T. and Fenwick, C. R. : The chemistry and biological significance of saponins in foods and feeding stuffs. CRC Crit. Rev. Food Sci. Nutr., 26, 27-135(1987)   DOI   ScienceOn
8 Agrwal, P. K., Jain, D. C., Gupta, R. K. and Thakur, R. S. : Carbon-13 NMR spectroscopy of steroidal sapogenins and steroidal saponins, Phytochemistry. 24, 2479-2496(1985)   DOI   ScienceOn
9 Halliwell, B. and Gutteridge, J. M. C. : Formation of a thiobarbituric acid-reactive substance from deoxyribose in the persence of iron salts. FEBS Lett., 128, 347-350(1981)   DOI   PUBMED   ScienceOn
10 Thurman, F. C., Ley, J. C. and Scholz, R. : Hepatic microsomal ethanol oxidation. Eur. J. Biochem., 25, 420-430(1972)   DOI   ScienceOn
11 Wang, H. and Murphy, P. A. : Isoflavone composition of American and Japanese soybeans in Iowa, Effects of variety crop tear and location, J. Agric. Food Chem., 42, 1674-1677(1994)   DOI   ScienceOn
12 Bogacheva, N. G., Kiselev, V. P. and Kogan, L. M. : Isolation of 3, 6-bisglycoside of yamogenin from Tgonella foemungaecum. Khim Prir Soedin 268-269(1976)
13 Liu, C. L., Chen, Y. Y., Ge, S. B. and Li, B. G. : Studies on the constituents from Cioscorec plants ll . Isolation and identification of steroidal saponins from Bi'oscoreg cojjeffii Hook F. Tfoxue fuefgo. 18, 597-606(1983)
14 Kudou, S., Tonomura, M., Tsukamoto, C., Shmoyamada M., Uchida, T. and Okubo, K. : Isolation and structural elucidation of main genuine soybean saponin. Biosci. Biotech. Biochem., 56(1), 142-143 (1992)   DOI   ScienceOn
15 Charmichael, J., Degroff, W. G., Gazdar, A. F., Minna, J. D. and Michell, J. B : Evalution of a terazolium based semiautonated colorimeteric assay, assessment of chemo sentivity testing, Cancer Res., 47,9366(1987)
16 Yoshicoshi, M., Yoshiki, Y., Okubo,K.,Sato,J.and Sasaki, Y. : Prevention of hydrogen peroxide damage by soybean saponins to mouse fibroflasts, Planta Medica, 6., 252-255(1996)
17 Shiraiwa, M., Kudou, S., Shimoyamada, M., Harada, K. and Okubo, K. : Composition and structure of 'group A'saponin in soybean seed. Agric. Biol. Chem., 55 (2), 315-22(1991)   DOI
18 남상해, 양민성 : 산국으로부터 항암활성 성분의 분리, 한국농화학회지, 38, 273(1995)
19 Tsukamoto, C., Kikuchi, A., Kudou, S., Tonomura, M., Harada, K., Iwasaki, T. and Okubo, K. : Genetic impovement of saponin components in soybean. ACS Symposium seres 546, Food Phytochemicals for Cancer Prevention, American Chemical Society, Washinton DC., USA, pp. 372-379(1994)
20 Shimoyamada, M., Suzuki, M., Sonta, H., Maruyama, M. and Okubo, K. : Antifungal Lctivity of the saponin fraction obtained from Asparagus officinalis L. and its active priciple, Agric. Biol. Chem., 54, 2553-2557(1990)   DOI
21 McCord, J. M. and Frdovch, I. : Superoxide dismutase. An enzyme funtion for erythro cuprein(heme cuprein). Chem. J. Biol., 244, 6049-6054(1969)
22 有地泫 :新規サポニン物質, 特許出願公開, 昭 56-64000 (1997)
23 Lowry, O. H., Roseborough, N. J., Farr, L. A. and Randall, R. J. : Protein measurement with Folin-Phenol reagent. J. Biol. Chem., 193, 265-275(1951)
24 Wei, H., Cai, Q. and Rahn, R. : Inhibition of UV light and Feton reaction-induced oxidative DNA damage by the soybean isoflavone genistein. Carcinogenesis, 17, 73(1996)   DOI   ScienceOn
25 이병우, 유익제, 유무영, 황우익, 최춘언 : 초산균체 추출물의 in vitro의 암세포 증식 억제효과, 한국식품과학회지, 27, 445(1995)
26 有地泫 : 代謝作用薬劑, 特許出願公開, 昭 56-160981 (1980)
27 Sharma, O. P., Adlercreutz, H., Strandberg, J. D., Zirkin, B. R., Coffey, D. S. and Ewing, L. L. : Soy of dietary source plays a preventive role against the pathogenesis of prostatitis in rats. J. Steroid Biochem. Mol. Biol., 43, 557-564(1992)   DOI   ScienceOn
28 Kawano, K., Sato, H. and Sakamura, S. : Isolation and structure of furostanol saponin in asparagus edible shoots. Agric. Biol. Chem., 41, 1-8(1997)