• Journal of Veterinary Clinics
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• v.8 no.1
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• pp.109-117
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• 1991

This experiment was carrid out to investigate whether Aloe arborescens had a radioprotective effect in cobalt-60 gamma irradiated(600 and 700ra4s) mice. The survival ratio of mice for 30 days and blood pictures were investigated. 1. The survival ratios of 600ra4s gamma Irradiated(dose rate 50rads/min.) in pre-irradiation aloe administered group(A), post-irradiation aloe administered group(B) and control group were 73.3%, 72.3% and 63.3%, and those of 700ra4s groups were 63.3%, 60.0% and 51.7%, respectively. 2. The total leucocyte counts of aloe administered groups were less decreased and more rapidly recovery to normal level than those of control group. 3. The lymphocyte counts were rapidly decreased on the 1st day after irradiation in all experimental groups than those of control group. 4. The recovery effects of total leucocyte counts of pre-irradiation aloe administered groups were superior to those of post-irradiation aloe groups and control group.

• Asian-Australasian Journal of Animal Sciences
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• v.15 no.4
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• pp.509-515
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• 2002

Copper deficiency was induced in eight male buffalo calves by adding molybdenum (30 ppm wet basis) to their diet. Copper status was monitored from the liver copper concentration and a level below 30 ppm (DM basis) was considered as deficient. Haemoglobin, haematocrit, total and differential leucocyte numbers were determined. The functions of peripheral neutrophils were assessed by in vitro phagocytosis and killing of Staphylococcus aureus. The effect of molybdenum induced copper deficiency on bone marrow was monitored. The mean total leucocyte count was unaffected whereas a significant fall in neutrophil count coincided with the fall in hepatic copper level to $23.9{\pm}2.69$ ppm. Reduced blood neutrophil numbers was not accompanied by any change in the proportion of different neutrophil precursor cells in bone marrow. It was hypothesised that buffalo calves were more tolerant to dietary molybdenum excess than cattle. It was concluded that neutropenia in molybdenum induced copper deficiency occurred without any effect on their synthesis and maturation process. Bone marrow studies in healthy calves revealed higher percentage of neutrophilic myelocytes and metamyelocytes as compared to cattle.

• Korean Journal of Poultry Science
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• v.45 no.3
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• pp.183-191
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• 2018

The study was conducted to investigate the effect of dietary supplementation of Lactobacillus, medicinal plants extract, and essential oil on laying performance, cecal microflora, relative organic weight, leucocyte profiles in laying hens against Salmonella gallinarum. A total of 100 Hy-Line Brown laying hens of 65 weeks of age were assigned into 5 dietary treatments supplemented with no antibiotic (negative control), antibiotic (positive control), Lactobacillus 0.1% (LB), medicinal plants extract 100 ppm (MPE), herb extract 100 ppm (EO). After 7 days of treatment, the 20 hens in each treatment group were divided into challenge group (n=10) and non-challenge group (n=10) for oral administration of Salmonella gallinarums, and were maintained in the same dietary treatments for two additional weeks. Laying hens were selected 20 by each treatment, splitting up into oral Salmonella group and nontreated group. The feeding trial lasted for one weeks and Salmonella gallinarum was orally administered for two weeks. Egg production, egg weight, egg mass, and feed conversion ratio were calculated and cecal microflora, relative organ, and blood were collected at the end of experiment. Compared with the control, laying hens fed Lactobacillus, medical plant extract, and essential oil were not observed to change of laying performance. Salmonella count increased significantly when Salmonella gallinarum was orally administered (P<0.05) but coliform bacteria and Lactobacillus counts were not affected. The relative organs weight was not different in hens with oral administration of Salmonella gallinarum and also in hens fed Lactobacillus, medical plant extract, and essential oil. H/L ratio was not significantly different, but total leucocyte level was somewhat increased in the treatment with oral administration of Salmonella gallinarum and more leucocyte increased in laying hens fed Lactobacillus, medical plant extract, and essential oil than control.

• The Korean Journal of Nuclear Medicine
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• v.33 no.4
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• pp.413-421
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• 1999

Purpose: This study was performed to evaluate the usefulness of $^{99m}Tc$-HMPAO-labelled leucocyte scintigraphy for diagnosing prosthetic infection after total knee replacement arthroplasty without the aid of following bone marrow scintigraphy Materials and Methods: The study subjects were 25 prostheses of 17 patients (one man and 16 women, mean age. 65 years) who had total knee replacement arthroplasty. After injection of $^{99m}Tc$-HMPAO-labelled leucocyte, the whole body planar and knee SPECT images were obtained in all patients. The subjects were classified into three groups according to clinical suspicion of prosthetic infection. Group A (n=11) with high suspicion of infection; Group B (n=6) with equivocal suspicion of infection, and Group C (n=8) with asymptomatic contralateral prostheses. Final diagnosis of infection was based on surgical, histological and bacteriological data and clinical follow-up. Results: Infection was confirmed in 13 prostheses (11 in Group A and 2 in Group B). All prostheses in Group A were true positive. There were two true positives, one false positive and three true negatives in Group B, and six true negatives and two false positives in Group C. Overall sensitivity, specificity, and accuracy for diagnosis of the infected knee prosthesis were 100%, 75% and 88%, respectively Conclusion: $^{99m}Tc$-HMPAO-labelled leucocyte scintigraphy is a sensitive method for the diagnosis of infected knee prosthesis. However, false positive uptakes even in asymptomatic prosthesis suggest that bone marrow scintigraphy may be needed to achieve improved specificity.

• Journal of fish pathology
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• v.18 no.3
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• pp.277-285
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• 2005

Israeli carps were intraperitoneally injected with an aqueous extract of red ginseng (RG) at five different concentrations (0, 1, 10, 25 and 50 mg/kg of body weight). The fish were sacrificed after 3 days and measured the neutrophil activity and plasma components. The activity of lysozyme, Ht, TIC and NBT reduction were significantly enhanced in the concentrations of more than 10 mg/kg. Further attempts were made to measure the effects of RG on leucocyte function at five different concentrations (1-100 ${\mu}g/m{\ell}$), in vitro. The proliferation of lymphocytes co-incubated with phytohaemagglutin (PHA) and concanavalin A (Con A) was significantly increased at 10 ${\mu}g/m{\ell}$ concentration, but suppressed at the highest concentration (100 ${\mu}g/m{\ell}$). RG alone or with lipopolysaccharide (LPS) had no effect on proliferation at any concentrations tested. Migration and reactive oxygen intermediates (ROI) production of leucocytes were enhanced in all concentration higher than 10 ${\mu}g/m{\ell}$. These results suggest that the RG activates the leucocyte function and may be able to be used as a stimulant of fish innate defences.

• Toxicological Research
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• v.7 no.1
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• pp.51-59
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• 1991

The purpose of the present study was to investigate the effects of non-watering, fasting and non-watering fasting for 16-17 hrs on haematological and biochemical parameters in SD rats. 1. Liver weight was decreased in the order of in the fasting group, non-watering fasting group and non-watering group in both sexes. 2. In fasting group and non-watering fasting group, haematologic parameters of male (HGB and MCH) and femal (MCHC) were trends to decrease compared with normal feeding & watering group. 3. In the differential leucocyte counts, there were no significant differences compared with noraml feeding % watering group.

• Korean Journal of Medicinal Crop Science
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• v.10 no.3
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• pp.167-170
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• 2002

Agromonia pilosa L. has been used as a medicinal plant in traditional folk remedy, and it shows increasing tendency at various sections such as medicine-making material, functional food, and agricultural chemicals using plant or extract. Dosage effect of extract from Agrimonia pilosa L. plant on rat performance experiments were summarized as follows : Body weight was increased with 0.02% dosage treated-rat by 5% as compared to non-treated one, however, decreasing tendency was observed with 0.04% extract of Agrimonia pilosa L. plant fed rat to control Considering feed efficiency was similar result between extract dosage with 0.02% and non-treated rat. The number of leucocyte was increased by dosage-treated level except 0.02% dosage-treated rat. Number of erythrocyte was increased with 0.06% extract dosage-treated rat by 20% as compared to non-treated one.

• The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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• v.19 no.3 s.31
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• pp.75-89
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• 2006

Background & Objectives : Rhinitis is an inflammation of nasal mucosa and the symtoms are watery rhinorrhea, sneezing, itchy nose, and nasal obstruction. Rhinitis is classified into allergic rhinitis and nonallergic. Allergic rhinitis is an immune reaction by allergen, and vasomotor rhinitis which is nonallergic noninfectious hypersensitive reaction. The incidence of allergic rhinitis has increased and the rate of vasomotor rhinitis is high. However there have been no studies about vasomotor rhinitis compared with allergic rhinitis. And there have been no studies so far performed on the effect of Tongkwansan. Therefore this study is aimed to find out the effects of Tongkwansan on allergic rhinitis and vasomotor rhinitis. Materials and Methods : Fifteen BALC/c mouses divided into three groups : normal group, control group and sample group. To induce the allergic rhinitis in control group and sample group, mouses were sensitized intrapertioneally 0.1 % ovalvumin solution three times at intervals of 1 week. Then intranasal sensitization was performed by diffusing 0.1 % ovalbumin solution 3 times at intervals of 2 days. After that time, mouses in the sample group were oral and administration treated by Tongkwansan for 28 days. We observed changes in nasal mucosa and submucosa; also changes in the segment of leucocyte, erythrocyte, neutrophil, lymphocyte, monocyte, eosinophil, IL-4 and $IFN-{\gamma}$ in blood. We used the statistical methods of ANOVA test(p<0.05). Results : There were no significant changes statistically in leucocyte, erythrocyte, neutrophil, lymphocytem, monocyte, eosinophil, IL-4 and $IFN-{\gamma}$ in blood(p<0.05). Hypertrophy of epithelium in nasal mucosa and expansion of glandular cells in nasal submucosa were decreased in treated group when compared with control group. Conclusion : According to above results, it is supposed that Tongkwansan has significant effects on vasomotor rhinitis which is nonallergic and noninfectious.

• Asian Pacific Journal of Cancer Prevention
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• v.15 no.8
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• pp.3641-3644
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• 2014

The present study aimed to determine the effect of oral ${\beta}$-glucan on mucositis and leukopenia in 62 consecutive patients with colorectal cancer treated with an adjuvant FOLFOX-4 regimen. The patients were retrospectively evaluated in 2 groups: one group received ${\beta}$-glucan and the other did not (control group). Leucocytes, neutrophils, and platelets were evaluated before and 1 week after chemotherapy and oral mucositis and diarrhea were noted. Leucocyte and neutrophil counts after chemotherapy in the ${\beta}$-glucan group were $7,300/mm^3$ and $3,800/mm^3$, respectively, and the reductions, as compared to baseline, were not significant (p=0.673 and 0.784). The median platelet count was $264,000/mm^3$ after chemotherapy in the ${\beta}$-glucan group and the reduction, as compared to baseline, was borderline significant (p=0.048). In the control group, reduction in leucocyte, neutrophil, and platelet counts was statistically significant. Oral mucositis and diarrhea were less common in the ${\beta}$-glucan group. We conclude that ${\beta}$-glucan can be used to reduce the adverse effects of chemotherapy.

• Asian-Australasian Journal of Animal Sciences
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• v.6 no.1
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• pp.147-153
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• 1993

A total of 109 heads of horses and ponies from eight horseback riding clubs nearby Central Taiwan were investigated to evaluate the blood parameters and the biochemical compositions of serum for the documentation of clinical pathological diagnosis and exercise physiology. Blood samples were collected from the jugular vein of resting horses. The sex difference in the blood traits were compared both in horses and in ponies. Results shows that total plasma proteins (PP) and hematocrit (PCV) were found higher in male horses than in females (p < 0.05). The sexual effect also exertes a significant influence on the leucocyte (WBC) count, but not on the erythrocyte (RBC) concentration. According to the differential counts of leucocytes, the number of monocytes and lymphocytes was higher in the male pony than that of in male horse. A close relationship Between the erythrocyte sedimention rate (ESR) and the other blood parameters were found especially in PCV, RBC concentration, and plasma protein level. The average ESR observed at 60 minutes were $21.80{\pm}21.87mm$, $39.50{\pm}18.90mm$ and $43.73{\pm}17.89mm$ in stallions, geldings, and mares, respectively. Most of the biochemical components of horse serum detected were distributed in normal ranges, although some of the items show a great variation in such a large sample size.