Korean Journal of Poultry Science (한국가금학회지)

The Korean Society of Poultry Science (한국가금학회)
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Effect of Dietary Lactobacillus, Medicinal Plants Extract, Herb Extract on the Laying Performance, Cecal Microflora, Relative Organs Weight, Leucocyte Profiles in Laying Hens against Salmonella gallinarum

Salmonella gallinarum에 대한 Lactobacillus, Medicinal Plants Extract, Essential Oil 급여가 산란계의 생산성, 맹장 내 미생물 균총, 조직중량 및 백혈구 조성에 미치는 영향

  • Kang, Hwan Ku (Poultry Research Institute, National Institute of Animal Science, Rural Development Administration) ;
  • Park, Seong Bok (Poultry Research Institute, National Institute of Animal Science, Rural Development Administration) ;
  • Kim, Hyun Soo (Poultry Research Institute, National Institute of Animal Science, Rural Development Administration) ;
  • Jeon, Jin Joo (Poultry Research Institute, National Institute of Animal Science, Rural Development Administration) ;
  • Park, Ki-Tae (Poultry Research Institute, National Institute of Animal Science, Rural Development Administration) ;
  • Kim, Chan Ho (Poultry Research Institute, National Institute of Animal Science, Rural Development Administration) ;
  • Hong, Eui Chul (Poultry Research Institute, National Institute of Animal Science, Rural Development Administration) ;
  • Kim, Sang Ho (Poultry Research Institute, National Institute of Animal Science, Rural Development Administration)
  • 강환구 (농촌진흥청 국립축산과학원 가금연구소) ;
  • 박성복 (농촌진흥청 국립축산과학원 가금연구소) ;
  • 김현수 (농촌진흥청 국립축산과학원 가금연구소) ;
  • 전진주 (농촌진흥청 국립축산과학원 가금연구소) ;
  • 박기태 (농촌진흥청 국립축산과학원 가금연구소) ;
  • 김찬호 (농촌진흥청 국립축산과학원 가금연구소) ;
  • 홍의철 (농촌진흥청 국립축산과학원 가금연구소) ;
  • 김상호 (농촌진흥청 국립축산과학원 가금연구소)
  • Received : 2018.07.10
  • Accepted : 2018.08.28
  • Published : 2018.09.30
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Abstract

The study was conducted to investigate the effect of dietary supplementation of Lactobacillus, medicinal plants extract, and essential oil on laying performance, cecal microflora, relative organic weight, leucocyte profiles in laying hens against Salmonella gallinarum. A total of 100 Hy-Line Brown laying hens of 65 weeks of age were assigned into 5 dietary treatments supplemented with no antibiotic (negative control), antibiotic (positive control), Lactobacillus 0.1% (LB), medicinal plants extract 100 ppm (MPE), herb extract 100 ppm (EO). After 7 days of treatment, the 20 hens in each treatment group were divided into challenge group (n=10) and non-challenge group (n=10) for oral administration of Salmonella gallinarums, and were maintained in the same dietary treatments for two additional weeks. Laying hens were selected 20 by each treatment, splitting up into oral Salmonella group and nontreated group. The feeding trial lasted for one weeks and Salmonella gallinarum was orally administered for two weeks. Egg production, egg weight, egg mass, and feed conversion ratio were calculated and cecal microflora, relative organ, and blood were collected at the end of experiment. Compared with the control, laying hens fed Lactobacillus, medical plant extract, and essential oil were not observed to change of laying performance. Salmonella count increased significantly when Salmonella gallinarum was orally administered (P<0.05) but coliform bacteria and Lactobacillus counts were not affected. The relative organs weight was not different in hens with oral administration of Salmonella gallinarum and also in hens fed Lactobacillus, medical plant extract, and essential oil. H/L ratio was not significantly different, but total leucocyte level was somewhat increased in the treatment with oral administration of Salmonella gallinarum and more leucocyte increased in laying hens fed Lactobacillus, medical plant extract, and essential oil than control.

본 시험은 Salmonella gallinarum에 대한 Lactobacillus, medicinal plants extract, essential oil 급여 시 산란계의 생산성, 맹장 내 미생물 균총, 조직중량 및 혈구 조성에 미치는 영향을 조사하기 위하여 수행하였다. 공시동물로 65주령 산란계(Hy-line brown) 총 100수를 공시하였고, 시험처리는 항생제 무첨가구(NC)와 항생제 첨가구(PC)를 대조구로 하였으며, 유산균 0.1% 첨가구(LB), 약용식물 추출물 100 ppm 첨가구(MPE) 및 식물 추출물 100 ppm 첨가구(EO)를 처리구로 두었다. 배치 후 1주일동안 시험 사료를 급여하고, 이후 Salmonella gallinarum을 경구투여하였다. 조사항목으로는 생산성, 맹장 내 미생물 균총, 조직중량 및 혈구 조성을 조사하였다. Salmonella gallinarum를 경구투여한 처리구와 투여하지 않은 처리구의 경우, Salmonella gallinarum를 경구투여한 처리구가 산란율, 1일 산란량 및 사료섭취량이 다소 감소하는 경향을 보였고, 유산균(LB), 약용식물 추출물(MPE) 및 식물추출물(EO)을 급여하였을 때 약용식물 추출물(MPE)을 급여한 처리구가 대조구에 비해 다소 개선되었지만 처리구간의 통계적인 유의차는 없었다. 맹장 내 미생물의 경우, Salmonella gallinarum을 경구투여하였을 때, Salmonella 수가 유의하게 증가하였으나(P<0.05), coliform bacteria 및 유산균 수에는 영향을 미치지 않았으며, Salmonella gallinarum과 유산균(LB), 약용식물 추출물(MPE), 및 식물추출물(EO) 사이의 상호작용도 나타나지 않았다. 조직중량에서도 Salmonella gallinarum 경구투여와 유산균(LB), 약용식물(MPE) 및 식물추출물(MPE) 첨가에 대한 통계적인 유의차는 없었고, 혈구 조성에서도 Salmonella gallinarum 경구투여와 유산균(LB), 약용식물 추출물(MPE) 및 식물추출물(EO) 첨가에 대한 유의차는 없었지만 각각 총 백혈구 수가 증가하는 경향을 보이기도 하였다.

Keywords

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