• Journal of Applied Biological Chemistry
• /
• 제66권
• /
• pp.492-502
• /
• 2023

This study investigated the antioxidant and immune enhancement activities of Artemisia argyi H. fermented by Lactobacillus plantarum. The fermented A. argyi H. ethanol extract increased scavenging activities of 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS⁺), hydroxyl (·OH), and superoxide (O₂^-) radicals. Particularly, the ethanol extract of fermented A. argyi H. exhibited higher ·OH and O₂^- radical scavenging activities, compared with DPPH and ABTS⁺ radical scavenging activities. To evaluate the immune enhancement effects of the fermented A. argyi H., mice were fed a normal diet supplemented the fermented A. argyi H. at concentrations of 1%, 2%, and 5%, respectively. The supplementation of fermented A. argyi H. dose-dependently increased splenocyte proliferation. In addition, mice fed with 5% fermented A. argyi H. showed enhanced proliferation of T-cells and B-cells, along with increased levels of interferon-γ, interleukin-10, and tumor necrosis factor-α, compared to the normal group. Furthermore, mice fed with fermented A. argyi H. exhibited an increase in prominent probiotics such as Akkermansia muciniphila and Lactobacillus in gut microbiota, compared to the normal group. This study suggests that fermented A. argyi H. with Lactobacillus plantarum could be used as a dietary antioxidant and immune enhancement agent.

• 농업과학연구
• /
• 제51권2호
• /
• pp.227-238
• /
• 2024

Methane (CH₄) is an important greenhouse gas, with a short-term greenhouse effect 80-fold that of carbon dioxide. Blast furnace slag used as a base ingredient for silicate fertilizer, and contained Fe³⁺, which acts as reduction of CH₄ emissions in flooded rice paddy. This study was evaluated the effects of the silicate fertilizer with different rates of the iron slag on CH₄ emissions and rice growth. In this study, the SF 0.0% was applied with silicate fertilizer containing 0.0% of the iron slag, while the SF 2.5% and SF 5.0% were treated with silicate fertilizer containing 2.5 and 5.0%, respectively. The CH₄ emissions during rice cropping period were assessed using a closed-chamber method and then determined by Gas chromatography. The CH₄ fluxes were reduced by 17% (SF 0.0%), 17% (SF 2.5%), and 8% (SF 5.0%) compared to the treatment with only-inorganic fertilization (control). Conversely, rice grain yield increased by 15 - 30% compared to the control owing to the improvement of soil quality by silicate fertilization. In particular, soil pH, available phosphorus and available silicic acid content were increased with the increase in the iron slag rates from 0.0 to 5.0%. These contributed to a significant increase in rice growth such as 1,000-grains weight and percentage of filled grains. Consequently, these findings were indicated that the application of silicate fertilizer containing 2.5 - 5.0% of iron slag would be the most effective in both CH₄ reduction and rice growth.

• 한국환경농학회지
• /
• 제13권1호
• /
• pp.47-59
• /
• 1994

유채재배시 기상조건에 의하여 유채의 발육속도 및 발육단계의 예측을 위하여 제주도 농촌진흥원에서 7년간($1982{\sim}1988$년) 실시한 유채 재배시기시험 및 작황시험성적을 분석하였다. 유채의 생육단계를 파종기, 추대기, 개화기, 성숙기로 나누어서 각 생육단계별로 온도와 일장의 함수로서 일 발육속도(DVR)를 구하고 그 적산치로서 발육단계(DVS)를 추정하여 실제 관측치와 추정치를 비교검토한 결과 다음과 같다. 1) 생육단계별 생육일수는 그 기간의 적산온도보다는 평균기온과의 상관이 높았다. 2) 생육단계별 구분없이 온도가 높고 일장이 길어지면 일 발육속도는 빨라지는 경향이었다. 3) 유채는 월동 후 기온이 높아지고 일장이 길어짐에 따라 생육이 활발해지며 일 발육속도는 경과일수에 따라 직선적으로 증가하는 경향을 보였다 4) 파종기부터 추대기까지의 일 발육속도는 $0{\sim}0.026$으로 시기별로 변화폭이 컸다. 5) 추대기에서 개화기까지의 일 발육속도는 $0.018{\sim}0.048$까지의 변화를 보였다. 6) 추대기에서 성숙기까지의 일 발육속도는 $0.005{\sim}0.018$까지의 변화를 보였다. 7) 각 생육시기별 발육단계 예측치는 실측치와 거의 일치하였으며, 상관계수는 $r=0.772{\sim}\;r=0.948$이었으며, 그 중 추대기에서 성숙기까지가 가장 높았다.

• 지질공학
• /
• 제20권1호
• /
• pp.25-33
• /
• 2010

연구지역의 오염된 대부분의 지하수는 상부의 오염된 천부지하수가 지하수공내로 유입되면서 질산성질소로 오염되었다. 공내 카메라 검층 결과 오염된 공들은 그라우팅 불량과 찢어진 유공관으로 인하여 오염된 천부지하수가 공내로 유입되어 지하수질을 악화시켰다. 오염된 천부지하수는 야외 지질조사시 확인된 동남동-서북서 방향, 북북서 방향, 북서-남동 방향, 남북 방향의 단층 및 절리, 파쇄대를 따라서 공내로 유입되었다. 금번 연구의 목적은 질산성질소로 오염된 지하수 공에 대하여 압축패커 기법을 이용하여 상부 오염물질 유입을 차단함으로써 지하수 수질개선 여부를 평가하는 것이다. 본 연구를 위하여 연구지역내 46개소의 지하수공을 대상으로 질산성질소 분석용 시료를 채취하였다. 질산성질소 함량이 10 mg/L 이상으로 먹는물 수질기준을 초과하거나 먹는물 수질기준에 가까운 공은 9개소로 전체 시료수의 약 20%이다. 압축패커 장치를 설치한 후 모든 공들의 질산성질소 함량이 설치 전에 비하여 26~81% 감소되었다.

• Molecules and Cells
• /
• 제41권6호
• /
• pp.582-590
• /
• 2018

Endothelial progenitor cells (EPCs) and outgrowth endothelial cells (OECs) play a pivotal role in vascular regeneration in ischemic tissues; however, their therapeutic application in clinical settings is limited due to the low quality and quantity of patient-derived circulating EPCs. To solve this problem, we evaluated whether three priming small molecules (tauroursodeoxycholic acid, fucoidan, and oleuropein) could enhance the angiogenic potential of EPCs. Such enhancement would promote the cellular bioactivities and help to develop functionally improved EPC therapeutics for ischemic diseases by accelerating the priming effect of the defined physiological molecules. We found that preconditioning of each of the three small molecules significantly induced the differentiation potential of $CD34^+$ stem cells into EPC lineage cells. Notably, long-term priming of OECs with the three chemical cocktail (OEC-3C) increased the proliferation potential of EPCs via ERK activation. The migration, invasion, and tube-forming capacities were also significantly enhanced in OEC-3Cs compared with unprimed OECs. Further, the cell survival ratio was dramatically increased in OEC-3Cs against $H_2O_2$-induced oxidative stress via the augmented expression of Bcl-2, a pro-survival protein. In conclusion, we identified three small molecules for enhancing the bioactivities of ex vivo-expanded OECs for vascular repair. Long-term 3C priming might be a promising methodology for EPC-based therapy against ischemic diseases.

• 농업과학연구
• /
• 제51권3호
• /
• pp.271-281
• /
• 2024

The application of biochar to soil for sustainable agriculture has been considered to have a positive impact on soil fertility and crop productivity. In this study, biochar derived from rice husk and chicken manure was applied to the soil at rates of 1% and 3% by weight, respectively, for lettuce cultivation experiments. The results indicated that both rice husk biochar and chicken manure biochar were effective in improving soil fertility through soil pH correction and increased nutrient content. The nitrogen content and pH of the biochar increased the available nitrogen and phosphate in the soil, creating a soil environment conducive to the growth enhancement of lettuce. In particular, after the application of 3% chicken manure biochar, the soil exhibited the highest levels of available nitrogen and phosphate at 87.42 mg·kg^-1 and 69.07 mg·kg^-1, respectively. Plant fresh weight increased with rising biochar amount, with the 3% chicken manure biochar treatment (228.25 g·plant^-1 fresh weight [FW]) exhibiting superior fresh weight compared to 3% rice husk biochar treatment (120.88 g·plant^-1 FW). The nutrient content in lettuce, except for K₂O, was higher in the chicken manure biochar treatment compared to the same dosage of rice husk biochar. Therefore, for enhancing lettuce productivity and soil fertility, chicken manure biochar appears to be more effective than rice husk biochar, and increasing the application rate up to 3% showed improvement effects without adverse impacts. However, excessive application of chicken manure biochar may lead to an increase in soil pH and electrical conductivity (EC) beyond the optimal range, requiring further assessment of application rates.

• 대한암한의학회지
• /
• 제15권1호
• /
• pp.29-36
• /
• 2010

The anti-metastatic activity of water extract of Samguikoeuitang (WSGKE) consisting of Ginseng Radix, Angelicae Radix, Sophora flavescens and Coicis Semenwas examined. Ethanol extract of Samguikoeuitang (ESGKE) showed significant cytotoxicity against colon 26-M3.1 carcinoma cells, while WSGEK did not. However, WSGKE significantly increased the production of IL-6 and IL-12 in thioglycollate-induced macrophages from Balb/c mice, whereas ESGKE did not. WSGKE significantly increased natural killer (NK) cell cytotoxicity against effecter YAC-1 cells in an Effecter cells/Target ratio dependent manner. Also, WSGKE significantly suppressed lung metastasis after i.v. injection of colon26-M3.1 carcinoma cells. Inhibitory effect of WSGKE on lung metastasis totally abolished in NK cells-deficient mice by treatment with anti-asialo GM1 serum. In addition, the combination treatment of cisplatin and WSGKE (100 ${\mu}g$/mouse) prolonged the lifespan of mice inoculated by colon26-M3.1 cell. These findings suggest that WSGKE can inhibit lung metastasis and prolong life span via immunological enhancement as a Biological Response Modifier.

• Advances in Traditional Medicine
• /
• 제9권2호
• /
• pp.115-127
• /
• 2009

Samsoeum (SSE) is used in traditional oriental medicine for various medicinal purposes. However, the exact mechanism that accounts for the anti-allergy and anti-inflammatory effects of the SSE is still not fully understood. The aim of the present study is to elucidate whether and how SSE modulates the allergic reactions in vivo, and inflammatory reaction in vitro. In this study, we showed that SSE significantly decreased compound 48/80-induced systemic anaphylaxis, ear-swelling response, histamine release from preparation of rat peritoneal mast cells and anti-dinitropheny IgE-induced passive cutaneous reaction. Also, SSE inhibited the expression of inflammatory cytokine and cyclooxygenase-2 in PMA plus A23187-stimulated human mast cells (HMC-1). In addition, we showed that anti-inflammatory mechanism of SSE is through suppression of nuclear factor-${\kappa}B$ activation and $I{\kappa}B-{\alpha}$ phosphorylation/degradation in HMC-1. These results provided new insight into the pharmacological actions of SSE as a potential molecule for therapy of inflammatory allergic diseases.

• 생물환경조절학회지
• /
• 제24권3호
• /
• pp.252-257
• /
• 2015

노지 포도재배는 강우에 직접 노출되어 열과와 병 발생이 많아진다. 그래서 도입된 것이 간이 비가림 재배이지만, 이것 역시 열과와 병 발생을 예방하는 데는 한계가 있다. 따라서 간이비가림 시설의 문제점을 보완하기 위해서 완전하게 강우를 차단하면서 자연적인 환기가 가능한 비가림 시설을 개발하여 적설하중과 풍하중을 내재 해 규격에 맞추었다. 개선된 비가림 시설의 특성을 검증하기 위해서 $100m^2$ 규모의 시설을 설치한 다음 관행 비가림 시설과 시설 내외부 온도 분포와 그에 따른 포도캠벨얼리의 품질, 갈반병 발생 및 열과율 등을 조사하여 비교하였다. 관행 비가림 시설과 개선된 비가림 시설의 온도 조사결과 외기온이 $34^{\circ}C$ 이상일 경우 시설 내부온도가 관행 최대 $40.7^{\circ}C$, 개선 시설은 $37.4^{\circ}C$로 측정되어 개선 시설이 3정도 낮았고, $32^{\circ}C$ 이하에서는 관행과 개선 시설 간에 온도 차이가 없었다. 과실 품질은 개선된 비가림 시설이 관행 비가림 시설보다 당도와 상품과율이 높았다. 개선된 비가림 시설이 관행에 비해 열과율과 갈반병 발병율이 현저히 낮았다.

• 한국작물학회:학술대회논문집
• /
• 한국작물학회 2017년도 9th Asian Crop Science Association conference
• /
• pp.368-368
• /
• 2017

Rice is the world's three major crops that is the staple food of Asian. Japonica type rice is consumed in Korea. Rice cultivation area decreases steadily. That is affected by decrease of the farm income with the inventory of 1.7 million tons, which is 2 to 3 times more than the optimal amount due to the decrease in the consumption of rice per person and cultivate high yield varieties. In recent years, as income level has improved, interest in health has been high and consumption for health food has been steadily increasing. For health food, rice is added by adding grains such as millet, sorghum, oats, beans and colored rice rather than white rice. In 1997, RDA(Rural Development Administration) developed black rice 'Heuknambyeo', and then 20 varieties were bred until 2017. In CBARES(Chungbuk Agricultural Research and Extension Services), we have developed new rice varieties 'Cheongpungheukchal' in 2010, 'Cheongpungheukhyangchal' in 2014, 'Cheongpungheukchal' is high in farming preference because of high yield. Black rice has high content of GABA and water-soluble phenol, DPPH radical scavenging activity, and consumers are interested in the function in the body. Therefore, functional and antioxidant activities(anthocyanin, total polyphenol, ${\alpha}-glucosidase$ inhibition) and antioxidant activities(ABTs, DPPH) were analyzed by comparing white and black rice. Testing varieties are 'Chucheongbyeo', best quality cultivars 'Daebo' and 'Samgwang' as white rice which are cultivated much in Chungbuk area, and black rice are 10 varieties including 'Cheongpungheukchal', 'Cheongpungheukhyangchal', 'Josaengheukchal' and so on. It has transplanted on 25th May, at CBARES research paddy by 100% fertilizer recommendation rate. Harvesting time was 50 days after heading by varieties, and has researched growth properties, yield and yield components, functional and antioxidant activities. Anthocyanin content was not measured because there was no pigmented in white rice, the highest value of anthocyanin content was 'Shintoheukmi' and in the range of 125.6~249.6mg/100g by black rice varieties. Total polyphenol content was high 'Cheongpungheukchal' and 'Shintoheukmi' and in the range of 96.68~244.34 mg/100g in black rice, white rice lower than blackish rice at 19.84~22.51mg/100g. ${\alpha}-glucosidase$ inhibition was high 'Cheongpungheukchal', 'Cheongpungheukhyangchal' and 'Shintoheukmi' in the rage of 75.87~98.85% by black rice varieties, especially 'Samgwang' was 80.75% and the other white rice was higher than 58~68%.