• KSBB Journal
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• 제24권3호
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• pp.273-278
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• 2009

본 연구는 김치에서 분리한 Lactobacillus plantarum YK-9의 다양한 특성을 조사하기 위하여 실시되었다. 형태학적 관찰 및 생리화학적 특성에 대하여 조사하였다. 16S rRNA 염기서열 분석을 통해 균주를 동정하였고, 그 결과 Lactobacillus plantarum로 확인되었고, L. plantarum YK-9으로 명명하였으며, 이 균주는 GenBank에 [FJ669130]로 등재하였다. 배양 시간에 따른 L. plantarum YK-9의 생장과 pH의 변화를 조사하였으며, 대사산물로서 유기산 (lactic acid와 acetic acid)의 생성은 HPLC를 통하여 확인하였다. 유기산의 생성은 L. plantarum YK-9의 생장에 따라 증가 하는 것을 확인하였다. 초기 pH 7.0은 배양 기간동안 3.6으로 감소하였고, 배양 72시간이 지난 후 생성된 lactic acid와 acetic acid의 농도는 각각 588.7 mM과 255.5 mM이었다. 식중독 원인균인 S. aureus, B. cereus, L. monocytogenes, E. coli, E. faecalis, S. enteritidis에 대하여 20배로 농축된 YK-9 배양 상등액에 처리하여 항균력을 조사하였으며, 실험에 사용된 6가지 식중독 원인세균 모두에 대한 항균효과가 있는 것이 관찰되었고, 이는 유기산 생성에 기인하는 것으로 나타났다.

• Journal of Microbiology and Biotechnology
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• 제16권10호
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• pp.1513-1517
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• 2006

One single lactic acid producing bacterium, isolated from kimchi, inhibited the growth and adherence of Helicobacter pylori to the human gastric epithelial cell line MKN-45. This isolate was identified as Lactobacillus plantarum and termed L. plantarum strain PL9011. The adherence of H pylori, in the presence of live or nonviable L. plantarum strain PL9011 (10-fold CFU), decreased to 14-20%. The spent culture supernatant of L. plantarum strain PL9011 resulted in the eradication of H pylori. This activity remained stable following neutralization and heat treatment, but not following pepsin treatment, thereby suggesting small peptides as the inhibitory factor. L. plantarum strain PL9011 did not produce any harmful metabolites or enzymes. The results obtained in this study suggest that the L. plantarum strain PL9011 may be a potential novel probiotic for the stomach.

• 한국축산식품학회지
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• 제34권5호
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• pp.647-655
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• 2014

Obesity is strongly associated with several metabolic and chronic diseases and has become a major public health problem of worldwide concern. This study aimed to investigate the physiological characteristics and anti-obesity effects of Lactobacillus plantarum Q180. Lactobacillus plantarum Q180 was isolated from the faces of healthy adults and found to have a lipase inhibitory activity of $83.61{\pm}2.32%$ and inhibited adipocyte differentiation of 3T3-L1 cells ($14.63{\pm}1.37%$) at a concentration of $100{\mu}g/mL$. The strain was investigated for its physiological characteristics. The optimum growth temperature of L. plantarum Q180 was $37^{\circ}C$. Lactobacillus plantarum Q180 showed higher sensitivity to novobiocin in a comparison of fifteen different antibiotics and showed the highest resistance to rifampicin, polymyxin B and vancomycin. The strain showed higher ${\beta}$-galactosidase and N-acetyl-${\beta}$-glucosaminidase activities. It also did not produce carcinogenic enzymes such as ${\beta}$-glucuronidase. The survival rate of L. plantarum Q180 in MRS broth containing 0.3% bile was 97.8%. Moreover, the strain showed a 97.2% survival rate after incubation for 3 h in pH 2.0. Lactobacillus plantarum Q180 was displayed resistance to Escherichia coli, Salmonella Typhimurium and Staphylococcus aureus with rates of 55.6%, 38.0% and 47.6%, respectively. These results demonstrate that L. plantarum Q180 has potential as a probiotic with anti-obesity effects.

• Journal of Microbiology and Biotechnology
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• 제24권12호
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• pp.1629-1635
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• 2014

It is known that lactic acid bacteria (LAB) have many beneficial health effects, including anti-oxidative activity and immune regulation. In this study, the immune regulatory effects of Lactobacillus sakei and Lactobacillus plantarum, which are found in different types of kimchi, were evaluated. L. sakei and its lipoteichoic acid (LTA) have greater immune stimulating potential in IL-12, IFN-${\gamma}$, and TNF-${\alpha}$ production as compared with L. plantarum in an in vitro condition. On the other hand, L. plantarum is assumed to repress the Th1 immune response in murine experiments. After being injected with LPS, L. plantarum-fed mice maintained a healthier state, and the level of TNF-${\alpha}$ in their blood was lower than in other bacterial strainfed mice and in the LPS-only control mice. Additionally, IL-12 production was significantly decreased and the production of IL-4 was greatly increased in the splenocytes from L. plantarum-fed mice. Further experiments revealed that the pre-injection of purified LTA from L. plantarum (pLTA), L. sakei (sLTA), and S. aureus (aLTA) decreased TNF-${\alpha}$ and IL-4 production in LPS-injected mice. Mouse IL-12, however, was significantly increased by aLTA pre-injection. In conclusion, the L. sakei and L. plantarum strains have immune regulation effects, but the effects differ in cytokine production and the regulatory effects of the Th1/Th2 immune response.

• 한국축산식품학회지
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• 제29권5호
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• pp.640-646
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• 2009

발효유제품, 김치, 및 신생아 분변 등에서 유산균을 분리하여 isoflavone 전환 능력을 평가하였다. 유산균 중 높은 수준의 $\beta$-glucosidase 활성을 보인 균주는 Enterococcus sp. 77, L. paracasei, Lactobacillus sp. 712, L. brevis ATCC 8287, Lactococcus sp. KU107, L. acidophilus KCNU, L. plantarum L155 등으로 나타났다. 이들 유산균에 대하여 유당 발효성을 평가하여 가장 우수한 유산균을 선발하여 API, 16S rDNA를 분석한 결과 Lactobacillus plantarum으로 동정되었으며 이 활성 유산균을 Lactobacillus plantarum YS712로 명명하였다. Lactobacillus plantarum YS712는 pH 2.5에서도 약 50% 이상의 강한 생존율을 보였으며 DPPH 라디칼 소거능도 95.8%로, 선발된 유산균 중 가장 높은 라디칼 소거능을 나타내었다. 이로써 Lactobacillus plantarum YS712는 발효식품 및 유제품에 다양하게 이용할 가치가 있다고 판단된다.

• 한국축산식품학회지
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• 제35권5호
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• pp.615-621
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• 2015

Lactobacillus plantarum FH185 was isolated from the feces of healthy adults. In our previous study, L. plantarum FH185 was demonstrated that it has anti-obesity effect in the in vitro and in vivo test. In order to determine its potential for use as a probiotic, we investigated the physiological characteristics of L. plantarum FH185. The optimum growth temperature of L. plantarum FH185 was 40℃. L. plantarum FH185 showed higher sensitivity to novobiocin in a comparison of fifteen different antibiotics and showed higher resistance to polymyxin B and vancomycin. It also showed higher β-galactosidase and N-acetyl-β-glucosaminidase activities. Moreover, it was comparatively tolerant to bile juice and acid, and inhibited the growths of Salmonella Typhimurium and Staphylococcus aureus with rates of 44.76% and 53.88%, respectively. It also showed high adhesion activity to HT-29 cells compared to L. rhamnosus GG.

• 한국식품과학회지
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• 제53권3호
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• pp.348-355
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• 2021

Bacteriophages (phages) are known determinants of kimchi microbial ecology. Lactobacillus plantarum is related to kimchi over-acidification during the late stages of kimchi fermentation. A phage infecting Lac. plantarum was isolated from kimchi and characterized. The phage population for kimchi in a market was 2.3 log particles/mL, which corresponded to 32% of the bacterial population on a log scale. The isolated phage was designated as ΦLP12116. ΦLP12116 which belonged to the Siphoviridae family and has a very narrow host range, infecting only Lac. plantarum. The phage was stable at a lactic acid concentration of 1.0% and pH 4.0 at 4℃, indicating that it could survive in kimchi. In the kimchi extract broth treated by the phage, the growth of Lac. plantarum KCCM 12116 was inhibited by 2.2 log CFU/mL compared to the growth in non-phage-treated broth. Therefore, this study suggests that the growth of Lac. plantarum, which is known as an acid-producing strain during late fermentation in kimchi, may be controlled using the phage.

• Journal of Microbiology and Biotechnology
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• 제26권5호
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• pp.975-987
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• 2016

Lactobacillus plantarum (L. plantarum) is a representative probiotic. In particular, L. plantarum is the first commensal bacterium to colonize the intestine of infants. For this reason, the initial settlement of L. plantarum can play an important role in determining an infant's health as well as their eventual health status as an adult. In addition, L. plantarum combats pathogenic infections (such as Escherichia coli (E. coli), one of the early pathogenic colonizers in an unhealthy infant gut) by secreting antimicrobial substances. The aim of this research was to determine how L. plantarum combats E. coli infection and why it is a representative probiotic in the intestine. Consequently, this research observed that E. coli releases uracil. L. plantarum specifically recognizes E. coli-derived uracil, which increases the growth rate and production of antimicrobial substance of L. plantarum. In addition, through the inhibitory activity test, this study postulates that the antimicrobial substance is a protein and can be considered a bacteriocin-like substance. Therefore, this research assumes that L. plantarum exerts its antibacterial ability by recognizing E. coli and increasing its growth rate as a result, and this phenomenon could be one of the reasons for L. plantarum settling in the intestine of infants as a beneficial bacterium.

• 한국축산식품학회지
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• 제22권1호
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• pp.81-86
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• 2002

쇠고기 분쇄육으로부터 박테리오신 생산균주를 분리하여 형태학적, 생화학적 특성을 조사한 결과 Lactobacillus plantarum ssp. 와 유사하게 나타났으며 당발효성 실험결과 95%의 신뢰도로 L. plantarum으로 동정되어 L. plantarum KU107로 명명하였다. L. plantarum KU107이 생산하는 박테이로신은 trypsin과 pepsin의 처리에 의해 활성이 소실되었으며 pH 2와 12에서도 활력이 완전하게 소실되지 않는 pH 안정성과 열안정성을 갖는 것으로 조사되었다. 또한, 이 박테리오신은 Bacillus cereus, Listeria inoccua, Listeria monacytogenes, Staphylococcus aureus, Staphylococcus intermedius KCCM 11806, 그리고 Yersinia enterocolitica에 대해서도 항균 활성을 나타내었다. S. aureus가 접종된 쇠고기 분쇄육에 박테리오신을 처리한 경우, 대조구와는 다르게 저장 7일째까지 접종된 S. aureus 초기균수와 유의적인 차이없이 성장을 지연시킴을 알 수 있었으며 저장 14일까지 대조구에 비해 유의적으로 현저히 적은 균수를 나타내었다.

• 한국축산식품학회지
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• 제27권3호
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• pp.363-370
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• 2007

김치의 L. plantarum의 생균제적 특성을 조사하기 위하여 김치에서 산생산과 성장능력 등이 우수한 25종의 Lactobacillus sp.를 분리하였고, API kit 분석에 의하여 L. plantarum을 동정하고 재차 16S rDNA 염기서열(99.9% 상동성)을 비교한 후 L. plantarum JK-01로 표기하였다. L. plantarum JK-01은 MRS broth에서 배양시 18시간 후 $2.9{\times}10^{10}CFU/ml$로 최대로 증식하는 빠른 성장특성을 나타냈으며 pH도 4.5로 조속히 하강하였다. 효소활성은 xylanase, amylase, protease, phytase의 순으로 높은 활성을 포함하고 있는 것으로 예측되었다. L. plantarum JK-01은 pH 2에서도 $1.36{\times}10^5CFU/mL$가 생존하였고, 1%의 담즙산에서도 약 $10^6CFU/mL$이상이 생존하여 내산성과 내담즙산성이 강한 것으로 나타났다. 또, $60^{\circ}C$에서도 $3.3{\times}10^3$ CFU/mL 정도로 생존하는 내열성이 있었으며, 대장균과 함께 배양시 18시간 후 대장균을 사멸시키는 것으로 나타났다. 이상의 결과로부터 분리한 L. plantarum JK-01은 생균제로서 충분히 이용가치가 있는 것으로 사료되었다.