한국축산식품학회지 (Food Science of Animal Resources)

  • 제29권5호
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  • Pages.640-646
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  • 2009
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  • 2636-0772(pISSN)
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  • 2636-0780(eISSN)
한국축산식품학회 (Korean Society for Food Science of Animal Resources)
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Isoflavone 비배당화 및 항산화 활성을 지닌 Lactobacillus plantarum YS712의 선발

Isolation and Partial Characterization of Isoflavone Transforming Lactobacillus plantarum YS712 for Potential Probiotic Use

  • 발행 : 2009.10.31
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초록

발효유제품, 김치, 및 신생아 분변 등에서 유산균을 분리하여 isoflavone 전환 능력을 평가하였다. 유산균 중 높은 수준의 $\beta$-glucosidase 활성을 보인 균주는 Enterococcus sp. 77, L. paracasei, Lactobacillus sp. 712, L. brevis ATCC 8287, Lactococcus sp. KU107, L. acidophilus KCNU, L. plantarum L155 등으로 나타났다. 이들 유산균에 대하여 유당 발효성을 평가하여 가장 우수한 유산균을 선발하여 API, 16S rDNA를 분석한 결과 Lactobacillus plantarum으로 동정되었으며 이 활성 유산균을 Lactobacillus plantarum YS712로 명명하였다. Lactobacillus plantarum YS712는 pH 2.5에서도 약 50% 이상의 강한 생존율을 보였으며 DPPH 라디칼 소거능도 95.8%로, 선발된 유산균 중 가장 높은 라디칼 소거능을 나타내었다. 이로써 Lactobacillus plantarum YS712는 발효식품 및 유제품에 다양하게 이용할 가치가 있다고 판단된다.

Lactic acid bacteria (LAB) are typical probiotic microbes that are used in various industries including fermented foods, feed additives, and pharmaceuticals. The purpose of this study was to compare the ability of isoflavone biotransformation and antioxidative activity of 17 LAB. Six strains including the Lactobacillus species exhibited a 100% hydrolysis rate for daidzein during fermentation. The content of total genistein in soymilk fermented with these strains was $872-943\;{\mu}g/g$. The DPPH (1, 1-diphenyl-2-picrylhydrazyl) radical scavenging ability of the LAB was widely variable and ranged from 23-78%. A selected strain was isolated from kimchi and the strain was identified as Lactobacillus plantarum ssp. through the API carbohydrate fermentation pattern and 16S rDNA profile. The strain exhibited excellent acid tolerance in an artificial gastric solution. L. plantarum YS712 showed high $\beta$-glucosidase activity in fermentation. The concentration of genistein and daidzein in soymilk fermented with L. plantarum YS712 increased from 3.64 to $917.3\;{\mu}g/g$ and from 58.18 to $1062.17\;{\mu}g/g$, respectively. These results demonstrate the potential of L. plantarum YS712 as a probiotic culture that can be utilized in the manufacturing of fermentation foods and dietary supplements.

키워드

참고문헌

  1. Adlercreuts, H. (1995) Phytoestrogens: epidemiology and a possible role in cancer protection. Environ. Health Persp. 103, 103-112 https://doi.org/10.1289/ehp.95103s2103
  2. Adlercreutz, H., Hockerstedi, K., Bannwart, C., Bloigu, S., Hamalainen, E., Fotsis, T., and Olles, A. (1987) Effect of dietary components, including lignans and phytoestrogens on enterohepatic circulation and liver metabolism of estrogens and on sex hormone binding globulin. J. Steroid Biochem. Mol. Biol. 27, 1135-1144 https://doi.org/10.1016/0022-4731(87)90200-7
  3. Adlercreutz, H., Mousavi, Y., Clark, J., Hockerstedt, K., Hamalainen, E., Wahala, K., Maketa, T., and Hase, T. (1992) Dietary phytoestrogens and cancer in vitro and in vivo studies. J. Steroid Biochem. Mol. Biol. 41, 331-337 https://doi.org/10.1016/0960-0760(92)90359-Q
  4. Aoshima, H., Tsunoue, H., Koda, H., and Kiso, Y. (2004) Aging of whisky increases 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity. J. Agr. Food Chem. 52, 5240-5244 https://doi.org/10.1021/jf049817s
  5. Blois, M. S. (1985) Antioxidant determination by the use of a stable free radical. Nature 4617, 1198
  6. Booth, I. R. (1985) Regulation of cytoplamic pH in bacteria. Microbiol. Rev. 49, 359-378
  7. Chien, H. L., Huang, H. Y., and Chou, C. C. (2006) Transformation of isoflavone phytoestrogens during the fermentation of soymilk with lactic acid bacteria and bifidobacteria. Food Microbiol. 23, 772-778 https://doi.org/10.1016/j.fm.2006.01.002
  8. Choi, Y. B., Woo, J. G., and Noh, W. S. (1999) Hydrolysis of $\beta$-glucosidase bonds of isoflavone conjugates in the lactic acid fermentation of soy milk. Kor. J. Food Sci. Technol. 31, 189-195
  9. Dunham, H. J., William, C., Edens, F. W., Casas, I. A., and Dobrogosz, W. J. (1993) Lactobacillus reuteri immunomodulation of stressor-associated disease in newly hatched chickens and turkeys. Poult. Sci. 72, 103
  10. Fuller, R. (1989) Probiotics in man and animals. J. Appl. Bacteriol. 66, 365-378
  11. Joo, J. C., Shin, J. H., Lee, S. J., Cho, H. S., and Sung, N. J. (2006) Antioxidative activity of hot water extracts from medicinal plants. J. Kor. Soc. Food Sci. Nutr. 35, 7-14 https://doi.org/10.3746/jkfn.2006.35.1.007
  12. Kang, Y. H., Park, Y. K., and Lee, G. D. (1996) The nitrite scavenging and electron donating ability of phenolic compounds. Kor. J. Food Sci. Technol. 28, 232-239
  13. Kim, H. K., Kim, Y. E., Do, J. R., Lee, Y. C., and Lee, B. Y. (1995) Antioxidative activity and physiological activity of some Korean medicinal plants. Kor. J. Food Sci. Technol. 27, 80-85
  14. Kim, H. J., Kim, J. H., Son, J. H., Seo, H. J., Park, S. J., Park, N. S., and Kim, S. K. (2004) Characterization of bacteriocin produced by Lactobacillus bulgaricus. J. Microbiol. Biotechnol. 14, 503-508
  15. Kim, N. Y. and Han, M. J. (2005) Development of Ginseng yoghurt fermented by Bifidobacterium ssp. Korean J. Food Cookery Sci. 21, 575-584
  16. Lee, I. S. and Park, K. Y. (2003) Preparation and quality characteristics of yoghurt added with cultured ginseng. Kor. J. Food Sci. Technol. 35, 235-241
  17. Lee, M. J., Sohn, C. Y., and Kim, J. H. (2008) Relation between health examination outcome and intake of soy food and isoflavone among adult male in seoul. Korean J. Nutr. 41, 254-263
  18. Lee, S. Y. and Oh, K. N. (1999) Effects of sweeteners and enzyme treatments on the quality attributes of soy yogurt containing soy protein isolate. Korean J. Soc. Food Sci. 15, 73-80
  19. Lin, M. Y. and Yen, C. L. (1999) Reactive oxygen species and lipid peroxidation product-scavenging ability of yogurt organism. J. Dairy Sci. 82, 1629-1634 https://doi.org/10.3168/jds.S0022-0302(99)75391-9
  20. Matsuda, S., Norimoto, F., Matsumoto, Y., Ohba, R., Teramoto, Y., Ohta, N., and Ueda, S. (1994) Solubilization of a novel isoflavone glycoside-hydrolyzing β-glucosidase from Lactobacillus casei subsp. rhamnosus. J. Ferment. Bioeng. 77, 439-441 https://doi.org/10.1016/0922-338X(94)90021-3
  21. Shah, N. P. (2006) Functional foods from probiotics and prebiotics. Food Technol. 55, 46-53
  22. Shanma, O. P., Adlercreutz, H., Strandberg, J. D., Zirkin, B. R., Coffey, D. S., and Ewing, L. L. (1992) Soy of dietary source plays a preventive role against the pathogenesis of prostatitis in rats. J. Steroid Biochem. Mol. Biol. 43, 557-564 https://doi.org/10.1016/0960-0760(92)90244-D
  23. Sim, J. H., Oh, S. J., Kim, S. K., and Baek, Y. J. (1995) Comparative tests on the acid tolerance of some lactic acid bacteria species isolated from lactic fermented products. Kor. J. Food Sci. Technol. 27, 101-104
  24. Tsangalis, D., Ashton, J. F., Magill, A. E. J., and Shah, N. P. (2002) Enzymic transformation of isoflavone phytoestrogens in soymilk by $\beta$-glucosidase-producing bifidobacteria. J. Food Sci. 67, 3104-3113 https://doi.org/10.1111/j.1365-2621.2002.tb08866.x
  25. Wei, H., Wei, L., Frenkel, F., Bowen, R., and Bames, S. (1993) Inhibition of tumor promoter-induced hydrogen peroxide formation in vitro and in vivo by genistein. Nutr. Cancer. 20, 1-12 https://doi.org/10.1080/01635589309514265
  26. Woo, J. Y., Paek, N. S., and Kim, Y. M. (2005) Studies on antioxidative effect and lactic acid bacteria growth of persimmon leaf extracts. Korean J. Food & Nutr. 18, 28-38

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  2. Probiotic Effects of Lactobacillus plantarum and Leuconostoc mesenteroides Isolated from Kimchi vol.45, pp.1, 2016, https://doi.org/10.3746/jkfn.2016.45.1.012
  3. Isolation and Characterization of Lactic Acid Bacteria with Angiotensin-Converting Enzyme Inhibitory and Antioxidative Activities vol.21, pp.10, 2011, https://doi.org/10.5352/JLS.2011.21.10.1428
  4. Characteristic Changes of Galgeuntang Fermented with Lactic Acid Bacteria vol.43, pp.5, 2011, https://doi.org/10.9721/KJFST.2011.43.5.655
  5. Characterization of Lactobacillus brevis SCML 432 isolated from Meju in Sunchang and optimization of its culture conditions by statistical methods vol.25, pp.3, 2018, https://doi.org/10.11002/kjfp.2018.25.3.397
  6. 베트남인 분변 및 김치로부터 분리된 유산균의 프로바이오틱스 기능성 연구 vol.35, pp.4, 2009, https://doi.org/10.22424/jmsb.2017.35.4.255
  7. Bioconversion of glycosides isoflavones to aglycone isoflavones by Lactobacillus rhamnosus BHN-LAB 76 under anaerobic conditions vol.26, pp.2, 2009, https://doi.org/10.11002/kjfp.2019.26.2.148
  8. Lactobacillus rhamnosus BHN-LAB 76에 의한 Pueraria 발효 추출물의 항산화 활성 평가 vol.29, pp.5, 2009, https://doi.org/10.5352/jls.2019.29.5.545
  9. Bioconversion of Isoflavone and Soyasaponin in the Fermentation of Soy Embryo Using Lactic Acid Bacteria vol.23, pp.3, 2009, https://doi.org/10.13050/foodengprog.2019.23.3.209
  10. Lactobacillus plantarum BHN-LAB 33의 생물전환공정을 통한 방풍 발효 추출물의 항산화 활성 및 미백 활성 증대 효과 vol.29, pp.11, 2019, https://doi.org/10.5352/jls.2019.29.11.1208