Browse > Article

Isolation of Lactobacillus plantarum from Kimchi and Its Inhibitory Activity on the Adherence and Growth of Helicobacter pylori  

Lee, Hak-Mee (Culture Collection of Antimicrobial Resistant Microbes, Department of Biology, Seoul Women's University)
Lee, Yeon-Hee (Culture Collection of Antimicrobial Resistant Microbes, Department of Biology, Seoul Women's University)
Publication Information
Journal of Microbiology and Biotechnology / v.16, no.10, 2006 , pp. 1513-1517 More about this Journal
Abstract
One single lactic acid producing bacterium, isolated from kimchi, inhibited the growth and adherence of Helicobacter pylori to the human gastric epithelial cell line MKN-45. This isolate was identified as Lactobacillus plantarum and termed L. plantarum strain PL9011. The adherence of H pylori, in the presence of live or nonviable L. plantarum strain PL9011 (10-fold CFU), decreased to 14-20%. The spent culture supernatant of L. plantarum strain PL9011 resulted in the eradication of H pylori. This activity remained stable following neutralization and heat treatment, but not following pepsin treatment, thereby suggesting small peptides as the inhibitory factor. L. plantarum strain PL9011 did not produce any harmful metabolites or enzymes. The results obtained in this study suggest that the L. plantarum strain PL9011 may be a potential novel probiotic for the stomach.
Keywords
Kimchi; lactic acid bacteria; Lactobacillus plantarum; Helicobacter pylori;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
Times Cited By Web Of Science : 12  (Related Records In Web of Science)
연도 인용수 순위
1 Atrih, A., N. Rekhif, A. J. Moir, A. Lebrihi, and G. Lefebvre. 2001. Mode of action, purification and amino acid sequence of plantaricin C19, an anti-Listeria bacteriocin produced by Lactobacillus plantarum C19. Int. J. Food Microbiol. 68: 93-104   DOI   ScienceOn
2 Cho, J., D. Lee, C. Yang, J. Jeon, J. Kim, and H. Han. 2006. Microbial population dynamics of kimchi, a fermented cabbage product. FEMS Microbiol. Lett. 257: 262-267   DOI   ScienceOn
3 Kim, M. and J. Chun. 2005. Bacterial community structure in kimchi, a Korean fermented vegetable food, as revealed by 16S rRNA gene analysis. Int. J. Food Microbiol. 103: 91-96   DOI   ScienceOn
4 Lee, C. H. 1997. Lactic acid fermented foods and their benefits in Asia. Food Control 8: 259-269   DOI   ScienceOn
5 Lee, H. Y., S. H. Seok, S. A. Cho, M. W. Baek, D. J. Kim, Y. Lee, and J. H. Park. 2004. Dietary intake of various lactic acid bacteria suppresses type 2 helper T cell production in antigen-primed mice splenocyte. J. Microbiol. Biotechnol. 14: 167-170   DOI   ScienceOn
6 Lee, Y. 2005. Characterization of Weissella kimchii PL9023 as a potential probiotic for women. FEMS Microbiol. Lett. 250: 157-162   DOI   ScienceOn
7 Sgouras, D., P. Maragkoudakis, K. Petraki, B. Martinez-Gonzalez, E. Eriotou, S. Michopoulos, G.. Kalantzopoulos, E. Tsakalidou, and A. Mentis. 2004. In vitro and in vivo inhibition of Helicobacter pylori by Lactobacillus casei strain Shirota. Appl. Environ. Microbiol. 70: 518-526   DOI
8 Holo, H., J. Jeknic, M. Daeschel, S. Stevanovic, and I. F. Nes. 2001. Plantaricin W from Lactobacillus plantarum belongs to a new family of two-peptide antibiotics. Microbiology 147: 643-651   DOI
9 Pinchuk, I. V., P. Bressollier, B. Verneuil, B. Fenet, I. B. Sorokulova, F. Megraud, and M. C. Urdaci. 2001. In vitro anti-Helicobacter pylori activity of the probiotic strain Bacillus subtilis 3 is due to secretion of antibiotics. Antimicrob. Agents Chemother. 45: 3156-3161   DOI   ScienceOn
10 Kim, T., S. H. Jung, J. Y. Lee, S. K. Choi, S. H. Park, J. S. Jo, and H. Y. Kim. 2003. Identification of lactic acid bacteria in kimchi using SDS-PAGE profiles of whole cell proteins. J. Microbiol. Biotechnol. 13: 119-124
11 Choi, S. Y. and L. Beuchat. 1994. Growth inhibition of Listeria monocytogenes by a bacteriocin of Pediococcus acidilactici M during fermentation of kimchi. Food Microbiol. 11: 301-307   DOI   ScienceOn
12 Choi, H. J., H. S. Lee, S. Her, D. H. Oh, and S. S. Yoon. 1999. Partial characterization and cloning of leuconocin J, a bacteriocin produced by Leuconostoc sp. J2 isolated from the Korean fermented vegetable kimchi. J. Appl. Microbiol. 86: 175-181   DOI   ScienceOn
13 Jur, J. W., T. H. Hyun, Y. R. Pyun, T. S. Kim, I. H. Yeo, and H. D. Paik. 2000. Identification and partial characterization of lacticin BH5, a bacteriocin produced by Lactococcus lactis BH5 isolated from kimchi. J. Food Prot. 63: 1707-1712   DOI
14 Lane, D. J. 1991. 16S/23S rRNA sequencing, pp. 115-175. In Goodfellow, M. (ed.), Nucleic acid Techniques in Bacterial Systematics. Willey, Chichester
15 Adolfsson, O., S. N. Meydani, and R. M. Russell. 2004. Yogurt and gut function. Am. J. Clin. Nutr. 80: 245-256   DOI
16 Chun, J. and M. Goodfellow. 1995. A phylogenetic analysis of the genus Nocardia with 16S rRNA gene sequences. Int. J. Syst. Bacteriol. 45: 240-245   DOI   ScienceOn
17 Nam, H., H. Misun, B. On, and Y. Lee. 2002. Effect of Weissella confusa strain PL9001 on the adherence and growth of Helicobacter pylori. Appl. Envir. Microbiol. 68: 4642-4645   DOI
18 Kabir, A. M. A., Y. Aiba, A. Takagi, S. Kamiya, T. Miwa, and Y. Koga. 1997. Prevention of Helicobacter pylori infection by lactobacilli in a gnotobiotic murine model. Gut 41: 49-55   DOI   ScienceOn
19 Choi, H. J., C. I. Cheigh, S. B. Kim, and Y. R. Pyun. 2000. Production of a nisin-like bacteriocin by Lactococcus lactis subsp. Lactis A164 isolated from kimchi. J. Appl. Microbiol. 88: 563-571   DOI   ScienceOn
20 Aiba, Y., N. Suzuki, A. M. Kabir, A. Takagi, and Y. Koga. 1998. Lactic acid-mediated suppression of Helicobacter pylori by the oral administration of Lactobacillus salivarius as a probiotic in a gnotobiotic murine model. Am. J. Gastroenterol. 93: 2097-2101   DOI
21 Coconnier, M. H., V. Lievin, E. Hemery, and A. L. Servin. 1998. Antagonistic activity against Helicobacter infection in vitro and in vivo by the human Lactobacillus acidophilus strain LB. Appl. Environ. Microbiol. 64: 4573-4580
22 Hamilton-Miller, J. M. 2003. The role of probiotics in the treatment and prevention of Helicobacter pylori infection. Int. J. Antimicrob. Agents 22: 360-366   DOI   ScienceOn