• Journal of Food Hygiene and Safety
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• v.9 no.1
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• pp.49-56
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• 1994

This study was carried out to investigate the levels of copper, zinc, manganese, nickel, cadmium and mercury content in breast milk among urban, rural and industrial lactating women in Korea. A total of 59 samples, which were collected from 17 in urban, 20 in rural and 22 in industrial area, and from 21-38 years-old healthy lactating women, were analyzed by Rigaku Mercury Analyzer for mercury, and by atomic absorption apectrophotometry for the other metals. The results are summarized as follows : The mean trace metal contents in breast milk were determined to be 0.34$\pm$0.14 ppm for copper, 2.01$\pm$1.43 ppm for zinc, 8.49$\pm$5.11 ppb for manganese, 7.75$\pm$5.73 ppb for nickel, 1.65$\pm$2.42 ppm for cadmium, 34.45$\pm$26.71 ppb for lead and 0.90$\pm$0.68 ppb for mercury. For the trace metal content in breast milk by area, the highest of copper, zinc, cadmium and mercury content were in urban, the highest of manganese content was in industrial, and the highest of nickel and lesd content were in rural. For copper, zinc, manganese and lead content in breast milk by lactation period, the highest levels were found in under 4 weeks after lactating, and subsequently the levels declined as lactation progressed, but the levels of zinc and manganese content increased from over 25 weeks after lactating. For cadmium and mercury content in breast milk by lactation period, the lowest levels were found in under 4 weeks after lactating, the highest levels were found in 5-12 weeks after lactating, and subsequently the levels declined as lactation progressed.For nickel content in breast milk by lactation period, the highest level was in 13-24 weeks after lactating, the lowest level was in 5-12 weeks after lactating.

• Korean Journal of Community Nutrition
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• v.1 no.1
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• pp.41-46
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• 1996

This study was undertaken to determine talc fatty acid intakes of lactating women and breast fed infants. Food consumptions of lactating women were measured at 3 4nd 9 days find 4, 8 and 12 weeks postpartum, respectively and diet samples were collected at the same time. Breast milk and blood samples were collected at 12 weeks postpartum. Fatty acid profiles of diet and breast milk were analyzed with GC. Total fatty acid intakes, fat percentage of energy, P/M/S and n-6/n-3 ratios of lactating women were 50.7 g/d, 20.9$\%$, 0.6/0.9/1 and 4.0/l, respect timely, over the first 12 weeks of postpartum and were not significantly different according to the lactating stages. Total fatty acid intakes and fat percentage of energy of the infects aged 12 weeks through the breast milk alone were 18.9 g/d and 44.8. This study shows that the linoleic acid percentage of energy intake in infants was 6.7$\%$, which was in optimum and/or high level to generally recommended guideline. DHA intakes of lactating women were positively correlated with those of infants from breast mil.

• Journal of Nutrition and Health
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• v.41 no.6
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• pp.518-529
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• 2008

Lactating women have an increased need of folate in the breastfeeding period and, as a consequence, may be in risk of folate deficiency. Folate content of breast milk, furthermore, is important for infants to support exponential growth. However, little is known about the folate content of breast milk from Korean lactating women and their folate nutritional status. In this study, therefore, we investigated the folate status of Korean lactating women and the folate content of their breast milk during extended lactation. A total of 10 subjects who delivered full-term infants participated this study voluntarily. Dietary folate intakes were measured and blood and breast milk were collected at 1, 2, 3, and 6 months postpartum. The women who did not take folic acid supplements failed to meet the recommended intake(RI) of folate for lactating women during all the study periods but those who did met the RI. The unsupplemented women showed lower plasma folate concentrations compared to the supplemented women and all the women were in suboptimal folate status determined by plasma folate concentration throughout the study periods. But the supplemented women showed lower prevalence of suboptimal folate status only at 3 or 6 months postpartum. Plasma folate concentrations of both groups decreased with the progression of lactation. Erythrocyte folate concentrations were not different between the two groups, however, that of the unsupplemented reduced further as time progressed. Plasma homocysteine levels were not different between the two groups. Concentrations of erythrocyte folate and plasma homocysteine were not changed throughout the study periods. Folate contents of their breast milk through the study periods were not different between the two groups and it decreased as lactation progressed in both groups. The results of this study suggest that the folate nutritional status of Korean lactating women might be deteriorated with the progression of lactation without folic acid supplements.

• Nutrition Research and Practice
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• v.11 no.1
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• pp.64-69
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• 2017

BACKGROUND/OBJECTIVES: This study was performed to measure fat-soluble vitamins and minerals in breast milk of Korean lactating mothers who exclusively breastfed their babies. SUBJECTS/METHODS: Breast milk samples were collected from 334 mothers. Concentrations of retinol and ${\alpha}$-tocopherol were analyzed by high performance liquid chromatography ultraviolet spectrometry while concentrations of minerals were measured by inductively coupled plasma optical emission spectrometry. RESULTS: Retinol and ${\alpha}$-tocopherol contents of breast milk were $39.58{\pm}19.64{\mu}g/dL$ and $0.23{\pm}0.13mg/dL$, respectively. Average sodium, potassium, calcium, phosphorus, and magnesium levels in breast milk were $11.11{\pm}5.16$, $38.56{\pm}9.01$, $27.87{\pm}6.10$, $13.56{\pm}3.30$, and $3.05{\pm}0.65mg/dL$, respectively. Contents of trace elements such as iron, zinc, copper, and manganese were $40.26{\pm}46.21$, $98.40{\pm}62.47$, $24.09{\pm}9.03$, and $0.90{\pm}1.63{\mu}g/dL$, respectively. Fat-soluble vitamin concentration was positively correlated with total fat in milk samples, but no significant differences were observed in levels of retinol, ${\alpha}$-tocopherol, or minerals based on whether or not lactating women were taking dietary supplements. CONCLUSIONS: Micronutrient contents of breast milk samples from Korean lactating women were comparable to those of other nations. Retinol and ${\alpha}$-tocopherol levels were correlated and also with total fat in breast milk.

• Journal of Nutrition and Health
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• v.32 no.1
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• pp.75-82
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• 1999

In order to investigate the longitudinal changes on zinc secretion of lactating women and zinc intake of breast-fed infants, we examined 20 lactating women(10 primipare and 10 multipare) and their infants during the first 90 days postpartum. We measured the consumed volume of human milk by test-weighing method and zinc concentration by atomic absorption spectophotometry after wet digestion. Weight gain of infants was -5.7, 54.1, 46.3, 42.0 and 32.3g/day at 7, 15, 30, 60 and 90 days postpartum, respectively. The secretion volume of human milk was 527, 608, 724, 841 and 798g/day respectively. The consumed volume of human milk of breast-fed infants was 432, 503, 603, 715 and 715g/day. The intake level of breast milk to secretion volume reached 85.0% in average. The zinc concentration of the milk was 4.29, 3.32, 2.52, 1.62 and 1.18mg/l, and the zinc intake of breast-fed infants was 1.80, 1.69, 1.45, 1.15 and 0.70mg/day. Zine intake per body weight of infants averaged 0.32mg/kg/day during the first 90 days postpartum. The average zinc intake of breast-fed infants was 1.36mg/day, which is 27.2% of the recommended daily allowance for 0-4-month-old infants.

• Journal of Nutrition and Health
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• v.29 no.2
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• pp.188-191
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• 1996

This study was undertaken to determine the effects of fish oil supplementation with low dose on the lipid content and fatty acid composition of breast milk. Among 18 lactating women who were exclusively breast-fed their babies, 6 were in control group and 12 were in fish lil groups. The subjects in fish oil groups were supplemented with 1.96g/d or 3.92g/d of fish oil for 2 weeks from 10 to 12 weeks of postpartum. All subjects consumed their usual diet at home. Breast milk samples were collected at the final day of experiment. By fish oil supplementation, the concentrations of triglyceride, cholesterol, free fatty acid and phospholipid as well as total lipid in breast milk tended to increase, but not significant. There was no dose-dependent response. The fatty acid composition of breast milk was not changed by fish oil supplementation. These results suggest that low dose of fish oil supplementation may increase of lipid content, but does not affect on the fatty acid composition of breast milk.

• Journal of Nutrition and Health
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• v.31 no.9
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• pp.1440-1445
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• 1998

In order to investigate the vitamin I intake of exclusively breast-fed infants, we examined 33 lactating women and their infants at 0.5, 1, 2, 3, 4 and 5 months of lactation. Vitamin E contents of human milk were determined by HPLC analysis. Vitamin E contents of the milk showed 539, 520, 464, 422, 409 and 351$\mu\textrm{g}$/100m1 during the lactation respectively. Vitamin E contents of the human milk were not affected by energy, lipid and protein consumption of lactating women. Vitamin E intake of breast-fed infants averaged 3375 and 269$\mu\textrm{g}$/day in boys and girls during the first 5 months of lactation. Vitamin E intake per body weight of breast-fed infants appeared 725, 752, 600, 461, 420 and 334$\mu\textrm{g}$/kg/day respectively. We conclude that breast-fed infants most likely receives adequate vitamin E from the human milk compared with recommended dietary allowances for Korean infants. (Korean J Nutrition 31(9) : 1440-1445, 1998)

• Korean Journal of Community Nutrition
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• v.12 no.3
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• pp.223-234
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• 2007

This study was done to determine the trans fatty acid (tFA) composition of human milk from postpartum to sixth months after delivery, to investigate the tFA intake of lactating women, and to estimate the intakes of tFA by infants exclusively fed breast milk. A total of 27 lactating Korean women participated to this study voluntarily, gave their breast milk, and responded to an investigation of their diets. The lactating women consumed 2.3-2.8 g/d of tFAs over the period of the first, second, third, and sixth months postpartum, which was 3.4-4.9% of the total fat intake and 0.8%-1.2% of the total energy intake. The proportions of tFAs in the breast milk were 1.89% in colostrum, 1.78% in transitional milk, and 1.78-2.25 in mature milk of the first, second, third, and sixth months postpartum. The tFAs of the breast milk identified in this study were C16:1n9t, C18:1n9t, C18:2n6t12t, C18:2n6t12c, C18:2n6t12t and C18:2n6t11t. Among them, C18:1n9t was predominant, which made up 59.26% of all tFAs in cob strum, 62.36% in transitional milk, and 64.42% in mature milk. The proportion of total tFA was unchanged with time, although some significant differences were noted for individual tFAs. The percentages of C18:2n6t12c and C18:2n6c12t decreased over the study period. Estimated tFA intake of the exclusively breast-fed infants was 0.18 g/d when fed colostrum, 0.29 g/d when fed transitional milk, and 0.53 g/d when fed mature milk until the sixth month of postpartum. Those were 0.5%, 0.8%, and 1.1% of the total energy intake. The results in this study indicate that lactating Korean women consume not a large quantity of tFAs, secrete breast milk not containing much tFA, and the estimated intake of tFAs by infants fed exclusively breast milk is not great.

• Journal of Nutrition and Health
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• v.28 no.8
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• pp.782-790
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• 1995

The longitudinal changes in protein secretion from 27 lactating women(primiparae = 10, multiparae=17) and protein intake of infants have been studied from 0.5 to 6 months postpartum in Chungju and Anseong area. The protein contents o breast milk in primiparae appeared significantly higher than in multipaae at 0.5 and 1 month postpartum. The protein secretion of primiparae and multiparae was not significantly different. In breast-feeding period, there was a tendency that protein secretion from 0.5 to 2 months postpartum was higher than thereafter. Average protein intake of boys from milk from 0.5 to 6 months postpartum was significantly higher than that of girls(p<0.05), because volume of milk intake of boys was much more than that of girls. In the amount of protein intake per infant weight, there was no significant difference between boys and girls. Protein intakes per infant weight decreased during lactation. Mean energy consumption of lactating women was 2,327㎉/day, which was 93.1% of recommended energy allowance for Koreans. Mean dietary protein consumption of lactating women was 81g/day, which was 101.3% of recemmended protein allowance for Koreans. Energy ratio of carbohydrate : protein : lipid was appeared to 68.8 : 13.9 : 17.3.

• Journal of Nutrition and Health
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• v.26 no.1
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• pp.76-88
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• 1993

Maternal body weight, skinfold thickness and circumference measurements were examined in 23 lactating women and 10 nonlactating women longitudinally from 1 week to 12 weeks postpartum. The dietary intakes of Korean lactating and nonlactating women were measured by 24-hour recall method and effects of maternal energy intake on body weight change were analyzed by Pearson correlation. The mean daily energy intake of breast-feeding(BF, n=12), combination of breast and formula-feeding (CF, n=11), and formula-feeding (FF, n=10) mothers were 1941kcal, 187kcal and 1727kcal, respectively. A significant decrease in weight was observed at all group. Weight losses at 12 weeks of postpartum in BF, CF and FF motheres were 3.18kg, 3.91kg and 5.15kg respectively. Weight losses increased as calorie intake decreased (${\gamma}$2=0.3803, p<0.05). The skinfold thickness and circumferences on all regions were decreased significantly except triceps and upper arm circumferences. However, there were no significant differences between lactating and nonlactating women. Anthropometric measurements decreased at the trunk but not at the limbs. Weight change was significantly correlated with changes of the scores for proximal circumference (${\gamma}$2=0.4999, p<0.05). There results suggest that lactation does not promete weight loss in well-nourished women and that the Korean RDA for energy in lactation may be too high.