To evaluated the usefulness of cultured human fibroblast for insulin receptor assay, the authors cultured fibroblast from biopsied normal adult female eyelid skin and assayed the insulin receptor with radioreceptor assay method. From the data obtained, percent of labeled insulin bound, numbers of insulin binding sites, affinity constants(Ka) and affinity of the empty sites(Ke) were calculated. The results were as follow; 1) The percent radioactivity bound of cultured fibroblast reached plateau at 4 hours $15^{\circ}C$ incubation. 2) The scatchard plot of insulin binding to cultured human fibroblast was curvilinear and the affinity to receptor was decreased with increased receptor occupancy. 3) The numbers of high affinity, low affinity and total insulin receptor of cultured fibroblasts were 852, 24,800 and 25,652 sites per cell. 4) High and low affinity constants of cultured fibroblasts were $3.4\times^{10}M^{-1},\;and\;1.08\times10^8M^{-1}$, and the affinity of empty site was $5.0\times10^8M^{-1}$.
KIPS Transactions on Software and Data Engineering
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v.5
no.1
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pp.13-20
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2016
In this paper, we propose an object recognition system that can effectively find out its category, its instance name, and several attributes from the color and depth images of an object with hierarchical feature learning. In the preprocessing stage, our system transforms the depth images of the object into the surface normal vectors, which can represent the shape information of the object more precisely. In the feature learning stage, it extracts a set of patch features and image features from a pair of the color image and the surface normal vector through two-layered learning. And then the system trains a set of independent classification models with a set of labeled feature vectors and the SVM learning algorithm. Through experiments with UW RGB-D Object Dataset, we verify the performance of the proposed object recognition system.
Objectives: The present study was performed to evaluate the potential effects of Bupleuri radix (SH) on regenerative activities in the peripheral sciatic nerve after crushing injury in rats. Methods: Axonal regeneration after crush injury in rats was analyzed by immunofluorescence staining using anti-NF-200 antibody and retrograde tracing of DiI-axons. Changes in protein levels in the sciatic nerve axons and DRG tissue were analyzed by Western blot analysis and immunofluorescence staining. Effects of SH extract treatment on neurite outgrowth was examined by immunofluorescence staining for cultured DRG neurons. Results: Major findings on the effects of SH extract treatment on axonal regeneration are summarized as follows. 1. SH-mediated enhancement in axonal regeneration was identified by immuno- fluorescence straining of NF-200 protein and retrograde tracing of DiI-labeled axons. 2. Axonal GAP-43 protein levels were upregulated by SH not only in the injured axons but also in the DRG sensory neurons corresponding to sciatic sensory axons. 3. Phospho-Erk1/2 protein levels were increased in both injured axonal area and DRG sensory neurons by SH. Phospho-Erk1/2 was also found in non-neuronal cells in the injured axons. 4. SH elevated levels of Cdc2 protein produced in Schwann cells in the distal portions of injured sciatic nerves. 5. The neurite outgrowth of DRG sensory neurons in culture was augmented by SH, and these changes were positively associated with GAP-43 production levels in the DRG neurons. Conclusions: These data suggest that SH extract improves the regenerative responses of injured peripheral neurons, and thus may be useful for understanding molecular basis for the development of therapeutic strategies.
Objectives : Alzheimer's disease(AD) is the most common form of dementia, which is characterized by progressive deterioration of memory and higher cortical functions that ultimately results in total degradation of intellectual and mental activities. Nokyongdaebo-tang(Lurongdabutang) has been usually used for the treatment for the deficiency syndrome dementia and amnesia. This experiment was designed to investigate the effect of the Nokyongdaebo-tang(Lurongdabutang) hot water extract on pathological AD model. Methods : The effects of the Nokyongdaebo-tang(Lurongdabutang) hot water extract on cultured spinal cord cells induced by ${\beta}$-amyloid were investigated. The effects of the Nokyongdaebo-tan(Lurongdabutang) hot water extract on the memory deficit mice induced by scopolamine were investigated. Results : 1. ${\beta}$-amyloid treatment on cultured spinal cord cells increased both GFAP-staining intensity of astrocytes and caspase 3 immunoreactivity on cultured cells. Then, Nokyongdaebo-tang(Lurongdabutang) treatment reduced the labeling intensity for both GFAP and caspase 3 proteins in culture cells. 2. Scopolamine treatment into mice increased levels of GFAP-positive astrocytes and caspase 3-labeled cells of the hippocampal subfields dentate hilar region, CA3 and CA1 area. In vivo administration of Nokyongdaebo-tang(Lurongdabutang) attenuated labeling intensity for those two proteins in the same hippocampal areas. Similar effects were observed by the treatment of galanthamine, an inhibitor of acetylcholinesterase. Conclusions : This experiment shows that the Nokyongdaebo-tang(Lurongdabutang) may play a protective role in damaged neural tissues. Since neuronal damage seen in degenerative brains such as AD are largely unknown, the current data may provide possible insight into therapeutic strategies for AD treatments. Nokyongdaebo-tang(Lurongdabutang) might be effective for the prevention and treatment of AD.
Proceedings of the Korean Society of Applied Pharmacology
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1996.11a
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pp.99-113
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1996
Taurine, a ${\beta}$-amino acid, plays an important role as a neuromodulator and is necessary for the normal development of the brain. Since de novo synthesis of taurine in the brain is minimal and in vivo studies suggest that taurine does not cross the blood-brain barrier, the blood-cerebrospinal fluid (CSF) barrier is likely to play a role in taurine transport between the central nervous system and the systemic circulation. Therefore, we examined in vivo elimination of taurine from the CSF in the rat to characterize in vivo kinetics of elimination for taurine from the CSF is consistent with the in vitro study. Using a stereotaxic device, cannulaes were placed into the lateral ventricle and the cisterna magna of the rat. Radio-labelled taurine and inulin (a marker of CSF flow) were injected into the lateral ventricle, and the concentrations of the labelled compounds in the CSF were monitored for up to 3 hrs in the cisterna magna. The apparent clearance of taurine from CSF was greater than the estimated CSF flow (p<0.005), indicating that there is a clearance process in addition to the CSF flow. Taurine distribution into the choroid plexus was at least 10 fold higher than that found in other brain areas (e.g., cerebellum, olfactory bulb and cortex). When unlabelled taurine was co-administered with radio-labelled taurine, the apparent clearance of the labeled taurine was reduced (p<0.01), suggesting a saturable disposition of taurine from CSF. Distribution of taurine into the choroid plexus, cerebellum, olfactory bulb and cortex was similarly diminished, indicating that the saturable uptake of taurine into these tissues is responsible for the non-linear disposition. A pharmacokinetic model involving first order elimination and saturable distribution described these data adequately. The Michaelis-Menten rate constant estimated from in vivo elimination study is similar to that obtained in the in vitro uptake experiment Collectively, our results demonstrate that taurine is transported in the choroid plexus via a taurine is cleared from the CSF via a saturable process. This process may be functionally relevant to taurine homeostasis in the brain.
Jo Hyun-Kyung;NamGung Uk;Seol In-Chan;Kim Yoon-Sik
Journal of Physiology & Pathology in Korean Medicine
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v.19
no.6
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pp.1666-1672
/
2005
Oriental medicinal drugs have a broad spectrum of clinical use for the cure of nervous system diseases including brain ischemic damages or neuropathies. Yet, specific drugs or drug components used in the oriental medicine in relation to none fiber regeneration are not known. In the present study, possible growth promoting effects of oriental medicinal drugs were investigated in the injured sciatic nerve system in the rat. By immunofluorescence staining, we found that Jahageo (JHG, Hominis placenta) increased Induction levels of axonal growth associated protein GAP-43 in the rat sciatic none. Small growth promoting activity was found in Golsebo (GSB, Drynariae rhizoma) and Baikhasuo (BHSO, Polygoni multiflori radix) drugs. JHG also increased cell cycle protein Cdc2 levels in the injured area of the sciatic nerves. Immunofluorescence staining indicated that induced Cdc2 protein was mostly localized in the Schwann cells in the injury area, implying that JHG activity might be related to increased Schwann cell proliferation during axonal regeneration. Moreover, levels of phospho-extracellular signal-regulated (ERK) pathway in the injured neNes were elevated by JHG treatment while levels of total ERK were unaltered. In vivo measurement of axonal regeneration using retrograde tracer showed that JHG, GSB and BHSO significantly enhanced Dil-labeled regenerating motor neurons compared with saline control. The present data suggest that oriental medicinal drugs such as JHG, GSB, and BHSO may be a useful target for developing specific drugs of axonal regeneration.
The present study was focussed to assess the proliferating cells in the distal epiphyseal tissue of the chicken femur by immunohistochemical staining methods. Four chickens were administrated intraperitoneally by twice consecutive injections, 1 day interval with bromodeoxyuridine(Brdur, 0.05 mg/gm BW/time), and then were killed by exsanguination of jugular vein at 2 hours after last injection. Samples were taken from femur distal epiphyseas of chicken. Labeling indexes(LI) were calculated as the ratio of the number of anti-Brdur monoclonal antibody-labeled cells in the each tissue layers from basal layer of the integument to bone marrow. The overall LI were found to be $13.90{\pm}3.44%$, $30.03{\pm}7.52%$, $16.00{\pm}9.41%$, $0.00{\pm}0.00%$ and $60.03{\pm}13.39%$ at basal layer of integument, perichordrium, reseving zone in cartilage, hypertrophic zone in cartilage and bone marrow respectively. LI in proliferating zone of cartilage were found to be $36.99{\pm}7.59%$, $32.83{\pm}5.38%$ and $22.02{\pm}6.27%$ at reserving zone side region, middle region, and hypertrophic zone side region respectively. The tissue layers with higher LI were odered as bone marrow, reserving zone side region in proliferating zone, middle region in proliferating zone, perichondrium, hypertrophic zone side region in proliferating zone. reserving zone of cartilage and basal layer of integument. These data indicate that the overall LI in the each tissue layer of distal epiphyseas of the chicken femur were concluded to be higher than that in another tissue of adult birds but hypertrophic zone of cartlage were appeared to be not proliferating cells.
Journal of the Korean Society of Clothing and Textiles
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v.25
no.2
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pp.303-314
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2001
The purposes of this study were 1) to identify different sub-segments of tourism market in Cheju island based on fashion image of clothing souvenir and 2) to provide manufactures with useful information for developing cultural fashion goods related to Cheju island. The data were collected via a self-administered questionnaire from 192 female tourist(20-59 years old) in Cheju and analyzed by factor analysis, cluster analysis, one-way ANOVA and $\chi$$^2$-test. Six factors of fashion image were found image were found and labeled as: Sexy/feminine, wild/mannish, easy/simple, Cheju, ecology, natural image. Four factors of benefit sought were also identified: Practicality/economic-value, fashionability/preference of design, reputation and souvenir-value. Three groups were identified based on fashion image sought: Image of primitive nature(G1: 37.7%), image of nature in the city(G2: 20.1%), sexy/feminine image(G3: 42.2%). There were significant differences among sub-groups in age, fashion innovation, benefit sought and intention of buying Gal-ot. Younger females(20-30 ages) were included more in group 1 than group 2 while older females(40-50 ages) were included more in group 2. Group 1 had a higher score of fashion innovation comparing group 3. Group 1 had the highest scores on practicality/economic-value and souvenir-value. However, group 2 placed the highest importance on reputation as well as practicality/economic-value and group 3 had the lowest scores on all types of benefit. Group 1 and group 2 had intention of buying Gal-ot more than group 1. Based on the results of this study, manufactures may implement target marketing strategies on group 1 which sought the image of primitive nature and group 2 which sought the image of nature in the city.
Journal of the Korea Society of Computer and Information
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v.10
no.1
s.33
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pp.59-66
/
2005
The organization of the web is progressively more being used to improve search and analysis of information on the web as a large collection of heterogeneous documents. Most people begin at a Web search engine to find information. but the user's pertinent search results are often greatly diluted by irrelevant data or sometimes appear on target but still mislead the user in an unwanted direction. One of the intentional, sometimes vicious manipulations of Web databases is a intentionally biased web page like Google bombing that is based on the PageRank algorithm. one of many Web structuring techniques. In this thesis, we regard the World Wide Web as a directed labeled graph that Web pages represent nodes and link edges. In the Present work, we define the label of an edge as having a link context and a similarity measure between link context and target page. With this similarity, we can modify the transition matrix of the PageRank algorithm. By suggesting a motivating example, it is explained how our proposed algorithm can filter the Web intentionally biased web Pages effective about $60\%% rather than the conventional PageRank.
This article studied the motivational factor of participation skill competition and their effectiveness. This study was restricted to cooks within those who had participated in a cooking contest to allow measurement of participation and satisfaction. This thesis investigated 116 cooks by questionnaires from April 6, 2005 to April 23, 2005, of which 106 responses were used for statistical processing to perform this study First, from the motive of participation, 13 variables were analyzed into 3 factors such as the factor of social recognition, the factor of speciality, and the factor of self-development and self-realization. The factor as a motive of participating in skill competition has a significant effect on effectiveness. A total of three factors were extracted from Recognition consisting of 10 variables. Each factor was labeled as awareness of the competition, revision of test questions, and selection of the judges of the competition. Second, three factors were derived from 12 variables in association with the effect of the contest, including factors of contribution to industry development, technical and technological effect, and psychological effect. In addition, it was revealed that the motive of participating in skill competition had an significant impact on recognition. Third, the results of regression analysis demonstrated that factors relating to recognition of skill competition and participation motives significantly influence on the effect of the contest. One limitation of this study is that the Population of the survey was limited to those who had Participated in the contest. Thus, the findings of this study may not be generalizable for other cases. It is clear that further research is needed to collect more in-dept data from other similar competitions in an effort to extend the application of the present results to other studies.
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