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Growth Promoting Effects of Oriental Medicinal Drugs on Sciatic Nerve Regeneration in the Rat  

Jo Hyun-Kyung (Department of Internal Medicine, College of Oriental Medicine, Daejeon University)
NamGung Uk (Department of Neurophysiology, College of Oriental Medicine, Daejeon University)
Seol In-Chan (Department of Internal Medicine, College of Oriental Medicine, Daejeon University)
Kim Yoon-Sik (Department of Internal Medicine, College of Oriental Medicine, Daejeon University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.19, no.6, 2005 , pp. 1666-1672 More about this Journal
Abstract
Oriental medicinal drugs have a broad spectrum of clinical use for the cure of nervous system diseases including brain ischemic damages or neuropathies. Yet, specific drugs or drug components used in the oriental medicine in relation to none fiber regeneration are not known. In the present study, possible growth promoting effects of oriental medicinal drugs were investigated in the injured sciatic nerve system in the rat. By immunofluorescence staining, we found that Jahageo (JHG, Hominis placenta) increased Induction levels of axonal growth associated protein GAP-43 in the rat sciatic none. Small growth promoting activity was found in Golsebo (GSB, Drynariae rhizoma) and Baikhasuo (BHSO, Polygoni multiflori radix) drugs. JHG also increased cell cycle protein Cdc2 levels in the injured area of the sciatic nerves. Immunofluorescence staining indicated that induced Cdc2 protein was mostly localized in the Schwann cells in the injury area, implying that JHG activity might be related to increased Schwann cell proliferation during axonal regeneration. Moreover, levels of phospho-extracellular signal-regulated (ERK) pathway in the injured neNes were elevated by JHG treatment while levels of total ERK were unaltered. In vivo measurement of axonal regeneration using retrograde tracer showed that JHG, GSB and BHSO significantly enhanced Dil-labeled regenerating motor neurons compared with saline control. The present data suggest that oriental medicinal drugs such as JHG, GSB, and BHSO may be a useful target for developing specific drugs of axonal regeneration.
Keywords
Hominis placenta; Polygoni Multiflori Radix; Drynariae Rhizoma; sciatic nerve; regeneration; GAP-43; Cdc2; ERK;
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  • Reference
1 Ide, C. Peripheral nerve regeneration. Neurosci Res 25, 101-121, 1996.
2 Fu, S.Y., Gordon, T. The cellular and molecular basis of peripheral nerve regeneration. Mol Neurobiol 14, 67-116, 1997.
3 Skene, J.H.P., Willard, M. Characteristics of growth associated polypeptides in regenerating toad retinal ganglion 'cell axons. J Neurosci 1, 419-426, 1981.
4 Xu, H., Jiang, B., Zhang, D., Fu, Z., Zhang, H. Compound injection of radix Hedysari to promote peripheral nerve regeneration in rats. Chin J Traumatol 5, 107-111, 2002.
5 Chen, Y.S., Wu, C.H., Yao, C.H., Chen, C.T. Ginsenoside RbI enhances peripheral nerve regeneration across wide gaps in silicone rubber chambers. Int J Artif Organs 25, 1103-1108, 2002.
6 Kim W.H, Choi W.Y. 臟腑辨證論治. 서울, 成保社, p 281-287, 1990.
7 Doree, M., Galas, S. The cyclin-dependent protein kinases and the control of cell division. FASEB J 8, 1114-1121, 1994.
8 Namikawa, K., Honma, M., Abe, K., Takeda, M., Mansur, K., Obata, T., Miwa, A, Okado, H., Kiyama, H. Akt/ protein kinase B prevents injury-induced motoneuron death and accelerates axonal regeneration. J Neurosci 20, 2875-2886, 2000.
9 Kishino, A., Nakayama, C. Enhancement of BDNF and activated-ERK immunoreactivity in spinal motor neurons after peripheral administration of BDNF. Brain Res 964, 56-66, 2003.
10 Desbarats, J., Birge, R.B., Mimouni-Rongy, M., Weinstein, D.E., Palerme, J.S., Newell, M.K. Fas engagement induces neurite growth through ERK activation and p35 upregulation. Nat Cell BioI 5, 118-125. 2003
11 Narngung, U., Routtenberg, A. Transcriptional and posttranscriptional regulation of a brain growth protein: regional differentiation and regeneration induction of GAP-43. Eur J Neurosci 12, 3124-3136, 2000.
12 Skene, J.H.P., Willard, M. Axonally transported proteins associated with axon growth in rabbit central and peripheral nervous system. J Cell Bioi 89, 96-103, 1981.
13 Liao, B., Newmark, H., Zhou, R. Neuroprotective effects of ginseng total saponin and ginsenosides RbI and Rg1 on spinal cord neurons in vitro. Exp Neural 173, 224-234, 2002.
14 전국한의과대학 본초학교수 共編著. 本草學. 서울, 永林社, pp 155-156, 287-288, 331-333, 545-546, 562-563, 567-568, 571-572, 583-584, 2000.
15 Gispen. W.H., Boonstra, J., De Graan, P.N.E., Jennekens, F. G.l.. Oestreicher, A.B., Schotman, P., Schrama, L.H., Verhaagen, J. and Margolis, F.L. B-50/GAP-43 in neuronal development and repair. Restorat Neurol Neurosci 1, 237-244, 1990.
16 Konishi, Y., Bonni, A The E2F-Cdc2 cell-cycle pathway specifically mediates activity deprivation-induced apoptosis of postmitotic neurons. J Neurosci 23, 1649-1658, 2003.
17 Lindsay, R.M., Wiegand, S.J., Altar, C.A., DiStefano, P.S. Neurotrophic factors: from molecule to man. Trends Neurosci 17, 182-190, 1994.
18 Konishi, Y., Lehtinen, M., Donovan, N., Bonni, A Cdc2 phosphorylation of BAD links the cell cycle to the cell death machinery. Mol Cell 9, 1005-1016, 2002.
19 O'Hare, M., Wang, F., Park, D.S. Cyclin-dependent kinases as potential targets to improve stroke outcome. Pharmacol Ther 93, 135-143, 2002.
20 Fawcett, J.W., Keynes, R.J. Peripheral nerve regeneration. Annu Rev Neurosci 13, 43-60, 1990.
21 Bornze, H.M., Bulsara, K.R., Iskandar, B.J., Caroni, P., Skene, J.H. Spinal axon regeneration evoked by replacing two growth cone proteins in adult neurons. Nat Neurosci 4, 38-43, 2001.
22 Davis, R.J. Signal transduction by the JNK group of MAP kinases. Cell 103, 239-252, 2000.
23 Cheng, Y.S., Cheng,W.C., Yao, C.H., Hsieh, C.L., Lin, J.G., La.i T.Y., Lin, C.C. Effects of buyang huanwu decoction on peripheral nerve regeneration using silicone rubber chambers. Am J Chin Med 29, 423-432, 2001.
24 Son, Y.J., Thompson, W.J. Schwarm cell processes guide regeneration of peripheral axons. Neuron. 14, 125-132, 1995.
25 Curtis, R., Adryan, K.M., Zhu, Y., Harkness, P.J., Lindsay, R.M., DiStefano, P.S. Retrograde axonal transport of ciliary neurotrophic factor is increased by peripheral nerve injury. Nature 365, 253-255, 1993.
26 Kim Y.S., Kim D.H., Seol I.C., Kim B.T. Study on the effect of Chukdamtang and Kamichukdamtang extract on brain damage and thromboembolism. Korean J Oriental Physiol & Pathol 15(1):92-104, 2001.
27 Al-Majed, A.A., Neumann, C.M., Brushart, T.M., Gordon, T. Brief electrical stimulation promotes the speed and accuracy of motor axonal regeneration. J Neurosci 20, 2602-2608, 2000.
28 Benowitz, L.I., Routenberg, A. A membrane phosphorylation associated with neuronal development, axonal regulation, phospholipid metabolism and synaptic plasticity. Trends Neurosci 10, 527-531, 1987.
29 Namgung, U., Choi, B.H., Park, S., Lee, J.D., Seo, H.S., Suh, B.C., Kim, KT. Activation of Cyclin-dependent kinase 5 is involved in axonal regeneration. Mol Cell Neurosci 25, 422-432, 2004.