• Mycobiology
• /
• 제46권3호
• /
• pp.236-241
• /
• 2018

The cation-dependent galactose-specific flocculation activity of the Schizosaccharomyces pombe null mutant of $lkh1^+$, the gene encoding LAMMER kinase homolog, has previously been reported by our group. Here, we show that disruption of $prk1^+$, another flocculation associated regulatory kinase encoding gene, also resulted in cation-dependent galactosespecific flocculation. Deletion of prk1 increased the flocculation phenotype of the $lkh1^+$ null mutant and its overexpression reversed the flocculation of cells caused by lkh1 deletion. Transcript levels of $prk1^+$ were also decreased by $lkh1^+$ deletion. Cumulatively, these results indicate that Lkh1 is one of the negative regulators acting upstream of Prk1, regulating non-sexual flocculation in fission yeast.

• Mycobiology
• /
• 제38권2호
• /
• pp.108-112
• /
• 2010

To investigate the possible roles of LAMMER kinase homologue, Lkh1, in Schizosaccharomyces pombe, whole proteins were extracted from wild type and lkh1-deletion mutant cells and subjected to polyacrylamide gel electrophoresis. Differentially expressed proteins were identified by tandem mass spectrometry (MS/MS) and were compared with a protein database. In whole-cell extracts, 10 proteins were up-regulated and 9 proteins were down-regulated in the mutant. In extracellular preparations, 6 proteins were up-regulated in the lkh1+ null mutant and 4 proteins successfully identified: glycolipid anchored surface precursor, $\beta$-glucosidase (Psu1), cell surface protein, glucan 1,3-$\beta$-glucosidase (Bgl2), and exo-1,3 $\beta$-glucanase (Exg1). These results suggest that Lkh1 is involved in regulating cell wall assembly.

• Mycobiology
• /
• 제51권5호
• /
• pp.372-378
• /
• 2023

Lkh1, a LAMMER kinase homolog in the fission yeast Schizosaccharomyces pombe, acts as a negative regulator of filamentous growth and flocculation. It is also involved in the response to oxidative stress. The lkh1-deletion mutant displays slower cell growth, shorter cell size, and abnormal DNA content compared to the wild type. These phenotypes suggest that Lkh1 controls cell size and cell cycle progression. When we performed microarray analysis using the lkh1-deletion mutant, we found that only four of the up-regulated genes in the lkh1-deletion were associated with the cell cycle. Interestingly, all of these genes are regulated by the Mlu1 cell cycle box binding factor (MBF), which is a transcription complex responsible for regulating the expression of cell cycle genes during the G1/S phase. Transcription analyses of the MBF-dependent cell-cycle genes, including negative feedback regulators, confirmed the up-regulation of these genes by the deletion of lkh1. Pull-down assay confirmed the interaction between Lkh1 and Yox1, which is a negative feedback regulator of MBF. This result supports the involvement of LAMMER kinase in cell cycle regulation by modulating MBF activity. In vitro kinase assay and NetPhosK 2.0 analysis with the Yox1T40,41A mutant allele revealed that T40 and T41 residues are the phosphorylation sites mediated by Lkh1. These sites affect the G1/S cell cycle progression of fission yeast by modulating the activity of the MBF complex.

• 한국운동역학회지
• /
• 제21권3호
• /
• pp.269-279
• /
• 2011

This study was analyzed the characteristics on the stability of posture while conducting a through two and half rotation technic of pench$\acute{e}$ in rhythmic gymnastics. Two rhythmical gymnastics player(LKH and SSJ) who is a member of the national team were selected, and for obtain the kinematic and kinetic variables were used a ProReflex MCU 240 infrared camera(Qualisys, Sweden) and a Type9286A force platform(Kistler, Switzerland). The mechanical factors were computed by using Visual3D program and Matlab R2009a. During the landing and rotation phase the results showed following characteristics; 1) In medial-lateral and horizontal displacement of the support foot, LKH showed smaller movement than SSJ, but SSJ showed smaller movement than LKH in swing foot. LKH showed bigger movement in medial-lateral axis of COP and vertical axis of COG, but SSJ showed bigger movement in horizontal axis of COP and medial-lateral axis of COG. 2) SSJ showed bigger maximum horizontal and vertical velocity at P1 and P2 than LKH. 3) In the inclination angle of COP and COG, SSJ showed smaller change than LKH, but within medial-lateral tilt of the shoulder, LKH performed rotation motion in horizontal position than SSJ. There was no differences in each force components during rotation, but on landing phase, the results showed a characteristic that SSJ exerted bigger breaking force and vertical force than LKH.

• 순환기질환의공학회:학술대회논문집
• /
• 순환기질환의공학회 2006년도 제6회 학술대회
• /
• pp.30-31
• /
• 2006

• Mycobiology
• /
• 제42권4호
• /
• pp.422-426
• /
• 2014

Depending on the acquisition of developmental competence, the expression of genes for ${\beta}$-1,3-glucan synthase and chitin synthase was affected in different ways by Aspergillus nidulans LAMMER kinase. LAMMER kinase deletion, ${\Delta}lkhA$, led to decrease in ${\beta}$-1,3-glucan, but increase in chitin content. The ${\Delta}lkhA$ strain was also resistant to nikkomycin Z.

• 한국정보처리학회:학술대회논문집
• /
• 한국정보처리학회 2010년도 추계학술발표대회
• /
• pp.1158-1161
• /
• 2010

사용자 그룹 서비스는 IPTV를 비롯한 다양한 분야에 걸쳐 응용되고 있으며, 안전한 그룹 통신을 위해 그룹 키를 사용한다. 그룹 키 관리를 보다 효율적으로 하기 위해 최근 많은 연구에서 트리를 이용한 기법을 제시하였다. 그 중 대표적인 기법은 LKH, OFT 등이 있으며, 이들은 그룹 키를 갱신하는데 필요한 메시지 전송량이 O(lgN) 수준이다. 이후 Sun, Kang 등은 키 갱신에 필요한 메시지 전송량을 O(1) 수준으로 감소시켜 더욱 효율적인 기법을 제안하였다. 본 논문에서는 이와 같은 기법들을 비교분석하여 효율성과 안전성에 대해 재조명하였다.

• 한국정보처리학회:학술대회논문집
• /
• 한국정보처리학회 2011년도 추계학술발표대회
• /
• pp.900-902
• /
• 2011

모바일 기기와 무선통신의 발달로 모바일 기기를 대상으로 하는 다양한 종류의 멀티캐스트 네트워크 기반 멀티미디어 서비스가 증가하고 있다. 이러한 멀티캐스트 통신 환경에서 보안성을 제공하기 위해 사용되는 그룹키를 전달하고 관리하는 것이 중요한 문제가 되고 있다. 또한 연구도 꾸준히 진행 되고 있으며, 본 논문은 기존연구(LKH)를 바탕으로 상대적으로 대역폭이 적은 모바일 환경에서 키-인덱스를 이용하여 키를 갱신하고 동기화하는 메시지의 양을 줄이는 방법을 제시한다.

• 한국식품저장유통학회지
• /
• 제15권5호
• /
• pp.760-768
• /
• 2008

EPS 생성 유산균인 Leu. kimchii GJ2의 생리활성 효능을 구명하기 위하여 고콜레스테롤식이를 급여한 흰쥐에게 Leu. kimchii GJ2 투여로 고지혈증 개선효과를 조사하였다. 체중증가율, 식이섭취량 및 식이효율은 실험군 간의 차이가 없었으나, 체중당 간의 무게는 고콜레스테롤식이군 (HCD)이 다른 실험군들에 비하여 유의하게 증가되었다. 고콜레스테롤식이 급여로 증가된 혈청 중 GOT 및 ALP 활성은 Leu. kimchii GJ2 투여 병합투여로 활성이 감소되었으며, Leu. kimchii GJ2 투여 용량이 증가할수록 농도 의존적으로 감소하였다. 혈청 중 중성지방과 인지질 함량은 실험군 간에 유의차가 없었으나, 총콜레스테롤 함량은 고콜레스테롤식이군(HCD군, HCD-LKL군 및 HCD-LKH군)들 간에 유의차를 보여 Leu. kimchii GJ2 투여 용량이 증가할수록 감소하였다. 고콜레스테롤식이와 Leu. kimchii GJ2 병합투여한 HCD-LKL군과 HCD-LKH군은 HCD군에 비하여 HDL-콜레스테롤 함량은 증가하였으나, LDL-콜레스테롤 함량, 심혈관위험지수 및 동맥경화지수는 유의하게 저하되었다. Leu. kimchii GJ2 투여 용량이 증가할수록 HDL-콜레스테롤 함량은 농도 의존적으로 증가하였고, LDL-콜레스테롤 함량은 저하되었다. 간조직 중 총콜레스테롤 및 중성 지방 함량은 HCD군이 ND군에 비하여 유의하게 증가되었으며, 고콜레스테롤식이와 Leu. kimchii GJ2 병합투여한 HCD-LKL군과 HCD-LKH군은 HCD군에 비하여 Leu. kimchii GJ2 투여 용량 의존적으로 저하되었다. 지방조직의 HR-LPL과 TE-LPL 활성은 HCD군이 다른 군들에 비하여 증가하였으며, Leu. kimchii GJ2 투여 용량이 증가할수록 HR-LPL과 TE-LPL 활성 모두 유의하게 저하되었다. 이상의 결과 Leu. kimchii GJ2은 고콜레스테롤식이 급여로 증가 되어진 총콜레스테롤 및 LDL-콜레스테롤 함량을 감소시키고, 감소되어진 HDL-콜레스테롤 함량을 증가시킴으로써 혈청 지질을 저하시키며, 간기능 보호효과가 있는 것으로 확인되어 고지혈증 예방과 치료에 효과가 있을 것으로 사료된다. Leu. kimchii GJ2의 이러한 효과는 Leu. kimchii GJ2가 EPS를 생성하여 유산균 본래의 기능인 장 운동 개선효과와 식이섬유소처럼 작용하여 지질의 장내에서의 흡수를 저해했기 때문이라 사료된다.

• KSII Transactions on Internet and Information Systems (TIIS)
• /
• 제13권7호
• /
• pp.3690-3713
• /
• 2019

According to the main group key management schemes logical key hierarchy (LKH), exclusion basis systems (EBS) and other group key schemes are limited in network structure, collusion attack, high energy consumption, and the single point of failure, this paper presents a group key management scheme for wireless sensor networks based on Lagrange interpolation polynomial characteristic (AGKMS). That Chinese remainder theorem is turned into a Lagrange interpolation polynomial based on the function property of Chinese remainder theorem firstly. And then the base station (BS) generates a Lagrange interpolation polynomial function f(x) and turns it to be a mix-function f(x)' based on the key information m(i) of node i. In the end, node i can obtain the group key K by receiving the message f(m(i))' from the cluster head node j. The analysis results of safety performance show that AGKMS has good network security, key independence, anti-capture, low storage cost, low computation cost, and good scalability.