Mycobiology

The Korean Society of Mycology (한국균학회)
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Possible Roles of LAMMER Kinase Lkh1 in Fission Yeast by Comparative Proteome Analysis

  • Cho, Soo-Jin (BioRefinery Center, Korea Research Institute of Chemical Technology) ;
  • Kim, Young-Hwan (Mass Spectrometry Research Center/Mass Spectrometry Team, Korea Basic Science Institute) ;
  • Park, Hee-Moon (Department of Microbiology and Molecular Biology, College of Bioscience and Biotechnology, Chungnam National University) ;
  • Shin, Kwang-Soo (Department of Microbiology and Biotechnology, Daejeon University)
  • Received : 2010.02.24
  • Accepted : 2010.03.06
  • Published : 2010.06.30
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Abstract

To investigate the possible roles of LAMMER kinase homologue, Lkh1, in Schizosaccharomyces pombe, whole proteins were extracted from wild type and lkh1-deletion mutant cells and subjected to polyacrylamide gel electrophoresis. Differentially expressed proteins were identified by tandem mass spectrometry (MS/MS) and were compared with a protein database. In whole-cell extracts, 10 proteins were up-regulated and 9 proteins were down-regulated in the mutant. In extracellular preparations, 6 proteins were up-regulated in the lkh1+ null mutant and 4 proteins successfully identified: glycolipid anchored surface precursor, $\beta$-glucosidase (Psu1), cell surface protein, glucan 1,3-$\beta$-glucosidase (Bgl2), and exo-1,3 $\beta$-glucanase (Exg1). These results suggest that Lkh1 is involved in regulating cell wall assembly.

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References

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