[KSCI] Korea Science Citation Index Service

http://dx.doi.org/10.4489/MYCO.2010.38.2.108

Possible Roles of LAMMER Kinase Lkh1 in Fission Yeast by Comparative Proteome Analysis

Cho, Soo-Jin (BioRefinery Center, Korea Research Institute of Chemical Technology)
Kim, Young-Hwan (Mass Spectrometry Research Center/Mass Spectrometry Team, Korea Basic Science Institute)
Park, Hee-Moon (Department of Microbiology and Molecular Biology, College of Bioscience and Biotechnology, Chungnam National University)
Shin, Kwang-Soo (Department of Microbiology and Biotechnology, Daejeon University)

Publication Information

Mycobiology / v.38, no.2, 2010 , pp. 108-112 More about this Journal

Abstract

To investigate the possible roles of LAMMER kinase homologue, Lkh1, in Schizosaccharomyces pombe, whole proteins were extracted from wild type and lkh1-deletion mutant cells and subjected to polyacrylamide gel electrophoresis. Differentially expressed proteins were identified by tandem mass spectrometry (MS/MS) and were compared with a protein database. In whole-cell extracts, 10 proteins were up-regulated and 9 proteins were down-regulated in the mutant. In extracellular preparations, 6 proteins were up-regulated in the lkh1+ null mutant and 4 proteins successfully identified: glycolipid anchored surface precursor, $\beta$ -glucosidase (Psu1), cell surface protein, glucan 1,3- $\beta$ -glucosidase (Bgl2), and exo-1,3 $\beta$ -glucanase (Exg1). These results suggest that Lkh1 is involved in regulating cell wall assembly.

Keywords

Lkh1; Proteome analyses; Schizosaccharomyces pombe;

Citations & Related Records

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