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http://dx.doi.org/10.1080/12298093.2018.1513115

LAMMER Kinase Lkh1 Is an Upstream Regulator of Prk1-Mediated Non-Sexual Flocculation in Fission Yeast  

Park, Yoon-Dong (Department of Microbiology & Molecular Biology, Chungnam National University)
Kwon, Soo Jeong (Department of Microbiology & Molecular Biology, Chungnam National University)
Bae, Kyung Sook (MicroID Co. Ltd)
Park, Hee-Moon (Department of Microbiology & Molecular Biology, Chungnam National University)
Publication Information
Mycobiology / v.46, no.3, 2018 , pp. 236-241 More about this Journal
Abstract
The cation-dependent galactose-specific flocculation activity of the Schizosaccharomyces pombe null mutant of $lkh1^+$, the gene encoding LAMMER kinase homolog, has previously been reported by our group. Here, we show that disruption of $prk1^+$, another flocculation associated regulatory kinase encoding gene, also resulted in cation-dependent galactosespecific flocculation. Deletion of prk1 increased the flocculation phenotype of the $lkh1^+$ null mutant and its overexpression reversed the flocculation of cells caused by lkh1 deletion. Transcript levels of $prk1^+$ were also decreased by $lkh1^+$ deletion. Cumulatively, these results indicate that Lkh1 is one of the negative regulators acting upstream of Prk1, regulating non-sexual flocculation in fission yeast.
Keywords
Non-sexual flocculation; LAMMER kinase; Lkh1; Prk1; negative regulator; Schizosaccharomyces pombe;
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