• Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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• v.38 no.3
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• pp.247-257
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• 2020

Livestock epidemics, such as foot and mouth disease, are causing enormous economic losses due to their strong infectious power. Early detection of infectious diseases in livestock is very important, but it is difficult to diagnose early in individual farms, and there are frequent cases of transmission through inter-farm movement such as veterinarians and feeding vehicles. In this study, we studied the technology that enables rapid diagnosis without veterinarian farm visits and prevents further spread by automatically monitoring the body temperature of livestock using ubiquitous-based information and communication technology in the early stage of onset and sending it in real time. We have presented a technique for systematically managing livestock epidemics at the farm level, regional level, and national level by using the community mapping technique by using the remote medical treatment system linked to the automatically collected information. In this process, community mapping items for each step and stakeholders were derived for crowd sourcing based spatial information technology.

• Journal of Microbiology and Biotechnology
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• v.13 no.4
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• pp.509-516
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• 2003

A model system for the immnunochemical detection of Escherichia coli O157:H7 using a combined immunomagnetic separation (IMS) and test-strip liposome immunoassay (LIA) procedure was developed. Immunomagnetic beads coated with anti-E. coli O157 IgG antibodies were used to separate the E. coli O157 (including the H7 serotype) from culture. Immunoliposomes, whose surface was conjugated to goat anti-E. coli O157:H7 IgG and which encapsulated the marker dye, sulforhodamine B, were used as a detection label. The test strip, onto which antibodies to goat IgG were immobilized, was the immunosensor capturing immunoliposomes that did not bind to E. coli O157:H7 on the immunomagnetic bead-E. coli O157:H7 complexes. In experiments, pure cell culture suspensions of $10^5 E.$ coli O157:H7 organisms per ml produced a measurable signal inhibition, whereas a weak yet detectable signal inhibition occurred with $10^3CFU/ml$. The inhibition signals increased, when the incubation time for IMS was extended to 90 min and higher IgG-tag density (0.4mol%) was used on the liposomes. With 0.2 and 0.4mol% IgG-tagged liposomes, the IMS-LIA procedure showed more improved signal inhibitions than those of a direct (no IMS) LIA. The combined assay, which measures the instantaneous signal from immunoliposomes, can be completed within 90 min, making it significantly faster than conventional plating methods and enzyme-linked immunosorbent assay (ELISA). Accordingly, it is quite feasible to use the combined immunoassay format of IMS and dye-loaded immunoliposomes for the detection of E. coli O157:H7.

• Proceedings of the Korean Institute of Navigation and Port Research Conference
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• 2009.10a
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• pp.206-207
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• 2009

It is estimated that the potential of Chinese market is infinite. The success of Chinese economic reformation is generating the high economic growth in China. China is a rising logistics market as its growing throughput and port expansion but China's railway transportation system is not well-linked with ports. China built TCR(Trans-China Railway) which is across the Chinese territory and connected to as far as Europe. With this mode, it is possible to place more overseas freight and meet more customers' needs. Lian yun gang is a city on the East coast of China where the commencing station of TCR is located. This paper reviews the present conditions of Lianyungang and presents the development direction of the city to become a logistics point.

• KSBB Journal
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• v.15 no.1
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• pp.42-48
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• 2000

Urokinase (UK), a thrombolytic enzyme used to clear catheters obstructed by blood clots, can be also used industrially in the recombinant protein purification system to cleave a fusion protein linked with a certain fragment of GST. We have immobilized UK by covalent attachment to activated Sepharose 6B-Cl gels and evaluated its performance to cleave a fusion protein of hGH and GST. The Sepharose gels were activated by etherification with glycidol (2,3-epoxypropanol) and further oxidized with periodate resulting in glyceryl-Sepharose gels. After the activation treatment, surface density of the aldehyde groups was 7-30 $\mu$mol-aldehde/mL-gel. Immobilization yield was higher than 99% at high pH (10.5), and the immobilized UK maintained ca. 80% specific activity of the soluble UK. In a column reaction the cleavage yield heavily depended on the feed rate, and it was nearly 86% of that from soluble UK. And the immobilized UK was successfully regenerated by unfolding and refolding with 6M GuHCl. After cleavaging reaction, the monomeric hGH was purified by using expanded bed adsorption chromatography.

• Journal of Navigation and Port Research
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• v.27 no.4
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• pp.479-484
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• 2003

In the case of the feeder bus route choice, it is more desirable to choose the route by treating as a single route linked from origin to destination than the whole network. This study is to establish a concept that frames the feeder bus route choice technique for the change of the existing single bus route or the creation of the new feeder bus route. The concept of the feeder bus route choice technique in this study is not to frame the whole bus network but to frame a single route to a unit O-D pair. So, this study has the assumption that does not consider the waiting and transfer time at the bus stop. This system technique consists of the following phases: I) limitation of the road network examined for the study, ii) enumeration of the appropriate candidate routes by the permissive route length, and iii) determination of the optimum bus route by the route evaluation value.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.7 no.6
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• pp.1356-1363
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• 2006

The main objective of this research is an increased understanding of how a supplier can successfully manage its care business in the mobile telecommunications market. In order to carry out this purpose, Uzbekistanis mobile telecommunication market has been studied as a case study in this research. The study tried to identify the forces and the factors present in CRM, and the role of these in enhancing (endangering) business. The objective was to gain a better understanding of how customer could be successfully managed and treated through CRM system. Processes and measures of customer satisfaction and loyalty provide two main aspects of the study. The importance of the concept of care and the actions that define it were found to be critically important for creating loyal customers. The relation between customer's needs, satisfaction, and loyalty, and how these ultimately relate to a providing firm's profitability, were seen to be linked in complex ways. The complexity can be studied in many ways but herein the customer satisfaction-loyalty of each event was first evaluated separately. Customer satisfaction and loyalty were then related to each other in order to compare the separate and combined characteristics.

• Journal of Korean Society of Rural Planning
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• v.25 no.4
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• pp.119-128
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• 2019

Although the General Agricultural Fishing Village Development Project had a significant impact on the landscape formation and management in rural area, the rural landscape planning and project sectors was not stable by fluctuation variables. On the basic understanding the institutional status of rural landscape planning, some conclusions could be discussed as follows through the analysis of quantitative and qualitative changes and the background factors. The rural landscape plan lacked its own legal and institutional foundations, and the landscape project of the General Agricultural Fishing Village Development Project was mainly focused on the environment-improving H/W project for expanding the basic living infrastructures. In order to reflect the characteristics of the region and become independent rural landscape management, it is necessary to change the contents so that the S/W business such as the deepening landscape awareness and empowerment linked with improving the physical landscape. To this end, it is necessary to establish a foundation for the rural landscape planning system to have its own status, and it seems to seek to supplement with what is included S/W contents reflecting the context of higher level landscape planning. The landscape sector of the General Agricultural Fishing Village Development Project proved to be in the process of shrinking in both quantity and quality, despite the temporary expansion in the past. In addition, considering the distortion of the contents of landscape projects in rural sites, it seems that the rural landscape-related public project are now in a position to seek a new phase change beyond quantitative expansion. In particular, along with institutional improvements to secure the independent status of rural landscape planning that contributes to village community regeneration and social sustainability through the role of intermediary support organizations in landscape management activities. Through the integrated landscape plan, which is established in advance and led by the residents in the early stages, there is a need to change the direction to enhance meaning of the landscape planning goes beyond the facility design or space improvement.

• Journal of Korean Society of Rural Planning
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• v.26 no.2
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• pp.117-126
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• 2020

In this study, demands of smart technology development were analyzed for rural village communities. Questionnaire items were derived by grasping the current status of information and communication technology. 49 villages in 8 regions were selected and surveys and statistical analysis were conducted. The main results of the study are as follows. First, 92% of community leaders use smartphones, search for information (38%), communicate with the Internet (36%) using smartphones, use KakaoTalk (31%), and Facebook (24%). Second, in the rural and urban exchange activities, promote support information service (51%) and promote method suggestion service (48.5%) showed that the demand for services in promote field was high. It is linked to the creation of economic opportunities. Third, in the income and production activities, demand for distribution services technology (39.3%) was high in the field of production and distribution, and cold chains that help maintain freshness until food, such as meat, fish, and vegetables are delivered to consumers when agricultural products are distributed. The constant temperature control system needs to be actively introduced. Fourth, autonomy activities showed the highest demand for air conditioning and control systems (34.2%) of community building, and the lowest demand for electronic voting (9.4%) and videoconferencing (9.4%) services. Lastly, in the general activity area of the community, the demand for technology of emergency services (37.1%) and health self-diagnosis service (35.4%), which are technologies in the welfare sector, ranked first and second respectively.

• Journal of the Korea Institute of Building Construction
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• v.11 no.5
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• pp.459-467
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• 2011

The work of constructing apartment housing involves various fields of industry that are linked to each other, and is based on a design document prepared by multiple technicians and architects. Consequently, design errors, material flaws or faulty construction works can cause defects, which sometimes overlap with each other. Construction companies should repair any defects found in a completed building within a specified period of time, and to do this, should establish a business plan by efficiently predicting the cost of defect repair. As it is very difficult for companies to accurately predict the occurrence of defects, historical performance data is used as a base. For domestic apartment housing units, data on the cost of defect repair is insufficient, so there are hardly any methods that can be used to make precise predictions. Therefore, the intent of this study is to develop a model that can predict the cost of defect repair by supply type and area, based on historical performance data with ten years worth of post-completion.

• The Plant Pathology Journal
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• v.17 no.2
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• pp.106-109
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• 2001

A multi-rapid immunofilter paper assay (multi-RIPA) system was prepared for simultaneous diagnosis of three Tobamoviruses, Cucumber green mottle mosaic virus (CGMMV), Kyuri green mottle mosaic virus (KGMMV), and Zucchini green mottle mosaic virus (ZGMMV) in cucurbitaceous crops. Each of these viruses was specifically detected by the multi-RIPA from cucumber, watermelon, zucchini, and bottle gourd inoculated with the three Tobamoviruses singly or in combination. The three viruses could infect cucumber, watermelon, and bottle gourd ; however, CGMMV could not infect zucchini as the latex-coated CGMMV antibody showed a negative reaction in the multi-RIPA of the virus-infected plant extract. When the minimum detection level of multi-RIPA was compared with that of double antibody sandwich-enzyme-linked immunosorbent assay (DAS-ELISA) using CGMMV, the latter was 10 times more sensitive than the former. The detection limit of the multi-RIPA for the purified CGMMV was 50 ng/ml. In a survey of the threeviruses in cucurbits growing in commercial fields in 1999 and 2000, CGMMV was detected in watermelon and cucumber, and ZGMMV was detected only in zucchini growing in plastic houses at the suburbs of Chonju, Korea. However, KGMMV was not found in the commercially growing cucurbit crops in our study, The results suggest that the multi-RIPA can be a simple, rapid, specific and convenient tool to detect simultaneously the Tobamoviruses.