• Proceedings of the Korean Biophysical Society Conference
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• 2003.06a
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• pp.44-44
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• 2003

$BK_{Ca}$ channels were suggested to contain one or more domains of the ‘regulator of K+ conductance’(RCK) in their cytosolic carboxyl termini (Jiang et al.2001). It was also shown that the RCK domain in mammalian $BK_{Ca}$ channels might sense the intracellular $Ca^{2+}$ with a low affinity (Xia et al. 2002). We aligned the amino acid sequence of the $\alpha$-subunit of rat $BK_{Ca}$ channels (rSlo) with known RCK domains and identified a second region exhibiting about 50％ homology. This putative domain, RCK2, contains the characteristic amino acids conserved in other RCK domains. We wondered whether this second domain is involved in the domain-domain interaction and the gating response to intracellular $Ca^{2+}$ for rSlo channel, as revealed in the structure of RCK domain of E. coli channel (Jiang et al.2001). In order to examine the possibility, site-directed mutations were introduced into the RCK2 domain of rSlo channel and the mutant channels were expressed in Xenopus oocytes for functional studies. One of such mutation, G772D, in the putative nucleotide-binding domain resulted in the enhanced $Ca^{2+}$ sensitivity and the channel gating of rSlo channel. These results suggest that this region of $BK_{Ca}$ channels is important for the channel gating and may form an independent domain in the cytosolic region of $BK_{Ca}$ channels. In order to obtain the mechanistic insights of these results, G772 residue was randomly mutagenized by site-directed mutagenesis and total 17 different mutant channels were constructed. We are currently investigating these mutant channels by electrophysiological techniques.ical techniques.

• BMB Reports
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• v.46 no.1
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• pp.37-40
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• 2013

CY-007 and CY-049 pteridine glycosyltransferases (PGTs) that differ in sugar donor specificity to catalyze either glucose or xylose transfer to tetrahydrobiopterin were studied here to uncover the structural determinants necessary for the specificity. The importance of the C-terminal domain and its residues 218 and 258 that are different between the two PGTs was assessed via structure-guided domain swapping or single and dual amino acid substitutions. Catalytic activity and selectivity were altered in all the mutants (2 chimeric and 6 substitution) to accept both UDP-glucose and UDP-xylose. In addition, the wild type activities were improved 1.6-4.2 fold in 4 substitution mutants and activity was observed towards another substrate UDP-N-acetylglucosamine in all the substitution mutants from CY-007 PGT. The results strongly support essential role of the C-terminal domain and the two residues for catalysis as well as sugar donor specificity, bringing insight into the structural features of the PGTs.

• KIPS Transactions on Computer and Communication Systems
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• v.1 no.1
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• pp.55-60
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• 2012

Recent botnets are widely using the DNS services at the connection of C&C server in order to evade botnet's detection. It is necessary to study on DNS analysis in order to counteract anomaly-based technique using the DNS. This paper studies collection of DNS traffic for experimental data and supervised learning for DNS traffic-based malicious domain classification such as query of domain name corresponding to C&C server from zombies. Especially, this paper would aim to determine significant features of DNS-based classification system for malicious domain extraction by the Principal Component Analysis(PCA).

• Journal of the Computational Structural Engineering Institute of Korea
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• v.17 no.1
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• pp.31-38
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• 2004

Mechanical design process is generally accomplished by design, analysis, and test. Designers use programs fitting purpose, and obtain repeatedly a response of a simulation program, a sub-program for optimization. In this paper, shape optimization using approximate optimization technique is carried out with sequential design domain(SDD). In addition, algorithm executing Pro/Engineer and ANSYS automatically are adopted in the approximate optimization program by SDD. It is difficult for design problem to be approximated accurately for the whole range of design space. However, more or less accurate approximation is constructed if SDD is applied to that case. SDD starts with a certain range which is off-seted from midpoint of an initial design domain and then SDD of the next step is determined by a move limited. Convergence criterion is defined such that optimal point must be located within SDD during the two steps. Also, the PLBA(Pshenichny-Lim-Belegundu-Arora) algorithm is used to solve approximate optimization problems. This algorithm uses the second-order information and the active set strategy, in order to seek the direction of design variables.

• Journal of the Computational Structural Engineering Institute of Korea
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• v.16 no.4
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• pp.431-437
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• 2003

In this paper, an approximate optimization program based on GUI(graphic user interface) environment is developed. This program is coded by using Fortran and Visual basic. Fortran is used to Progress approximate optimization process. Visual basic is used to make user environment for user to use conveniently. Inside of this program, it uses two independent programs. One is commercial program, ANSYS, and the other is optimization program, PLBA(Pshenichny-Lim-Belegundu Arora). The former is used to obtain approximate equation of stress and displacement of a structure. The latter is used to solve approximate optimization. This algorithm uses second-order information of a function and active set strategy. This program is connecting ANSYS and PLBA. And it progress the process repeatedly until it obtain optimum value. As a method of approximate optimization, sequential design domain(SDD) is introduced. SDD starts with a certain range which is offseted from midpoint of an initial design domain and then SDD of the next step is determined by optimal point of a prior step.

• 한국생물공학회:학술대회논문집
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• 2003.10a
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• pp.534-534
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• 2003

• International Journal of Industrial Entomology and Biomaterials
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• v.9 no.1
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• pp.149-153
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• 2004

Here we report the molecular cloning of a LIM protein cDNA of the CRP (cysteine-rich protein) family from the mulberry longicorn beetle, Apriona, geramri. The A. germari LIM protein cDNA contains an open reading frame of 276 bp encoding 92 amino acid residues with a calculated molecular weight of approximately 10 kDa. The A. germari LIM protein contains the cysteine-rich consensus sequence of LIM domain and the glycine-rich consensus sequence observed in cysteine-rich protein family 1 (CRP1). The potential nuclear targeting signal is retained. The deduced amino acid sequence of the A. germari LIM protein cDNA showed 81 % identity to both Bombyx mori muscle LIM protein (Mlp) and Drosophila melanogaster Mlp60A and 77% to Epiblema scudderiana Mlp. Northern blot analysis showed that A. germari LIM protein is highly expressed in epidermis and muscle, and less strongly in midgut, but not in the fat body.

• International Journal of Industrial Entomology and Biomaterials
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• v.11 no.1
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• pp.71-74
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• 2005

A LIM protein gene homologue of the CRP (cysteine­rich protein) family in the whiter-spotted flower chafer, Protaetia brevitarsis, was cloned. The P. brevitarsis LIM protein cDNA encodes a 92 amino acid polypep­tide with a predicted molecular mass of 10,030 Da and a pI of 8.57. The P. brevitarsis LIM protein contains the cysteine-rich consensus sequence of LIM domain and the glycine-rich consensus sequence observed in the cysteine-rich protein family 1 (CRPl). The potential nuclear targeting signal is retained. The deduced amino acid sequence of the P. brevitarsis LIM protein cDNA showed 92$\%$ identity to another beetle, Apriona germari LIM protein. Northern blot analysis showed that P. brevitarsis LIM protein is highly expressed in epidermis and midgut, but not in the fat body.

• Proceedings of the Korea Committee for Ocean Resources and Engineering Conference
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• 2004.05a
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• pp.167-171
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• 2004

The Spar platform with deep draft is characterized as effective structure in extreme wave condition, which has larger natural period than that of waves in sea. In this paper, the time simulation of motion responses of Spar with catenary mooring line is presented in irregular waves. The memory effect is modeled by added mass at infinite frequency and convolution integrals in terms of wave damping coefficients. The added mass, wave damping coefficients and wave exciting forces are obtained from three-dimensional panel method in the frequency domain. The motion equations are consisted of forces for inetia, memory effect, hydrostatic restoring, wave exciting and mooring line. The forces of mooring line are modeled as quasi-static catenary cable.

• Proceedings of the IEEK Conference
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• 2001.09a
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• pp.657-660
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• 2001

In this paper, a robust watermarking technique is presented by using a block-based statistics in DCT domain. First, the proposed technique calculates JND threshold value using the global statistics in DCT domain. Then watermark insertion is carried out by inserting one watermark into coefficients which are above the threshold value J within a 2${\times}$2 block. Finally, watermark is estimated by averaging the extracted watermarks from the coefficients which are above the threshold in a window. In experiments it is shown that the proposed techniques can enhance perceptual invisibility and robustness against additive noise and JPEG compression attacks by using the characteristics of JND.