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A LIM Protein Gene Homologue of Protaetia brevitarsis: cDNA Cloning and mRNA Expression  

Kim Iksoo (Department of Agricultural Biology, National Institute of Agricultural Science & Technology)
Choi Yong Soo (College of Natural Resources and Life Science, Dong-A University)
Lee Sun Young (Department of Agricultural Biology, National Institute of Agricultural Science & Technology)
Kim Mi Ae (Department of Agricultural Biology, National Institute of Agricultural Science & Technology)
Kim Seong Ryul (Department of Agricultural Biology, National Institute of Agricultural Science & Technology)
Hwang Jae Sam (Department of Agricultural Biology, National Institute of Agricultural Science & Technology)
Jin Byung Rae (College of Natural Resources and Life Science, Dong-A University)
Publication Information
International Journal of Industrial Entomology and Biomaterials / v.11, no.1, 2005 , pp. 71-74 More about this Journal
Abstract
A LIM protein gene homologue of the CRP (cysteine­rich protein) family in the whiter-spotted flower chafer, Protaetia brevitarsis, was cloned. The P. brevitarsis LIM protein cDNA encodes a 92 amino acid polypep­tide with a predicted molecular mass of 10,030 Da and a pI of 8.57. The P. brevitarsis LIM protein contains the cysteine-rich consensus sequence of LIM domain and the glycine-rich consensus sequence observed in the cysteine-rich protein family 1 (CRPl). The potential nuclear targeting signal is retained. The deduced amino acid sequence of the P. brevitarsis LIM protein cDNA showed 92$\%$ identity to another beetle, Apriona germari LIM protein. Northern blot analysis showed that P. brevitarsis LIM protein is highly expressed in epidermis and midgut, but not in the fat body.
Keywords
cDNA cloning; Insect; LIM protein; Prota­etia brevitarsis;
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