Altered sugar donor specificity and catalytic activity of pteridine glycosyltransferases by domain swapping or site-directed mutagenesis |
Kim, Hye-Lim
(School of Biological Sciences, Inje University)
Kim, Ae Hyun (School of Biological Sciences, Inje University) Park, Mi Bi (School of Biological Sciences, Inje University) Lee, Soo-Woong (Advanced Research Center for Multiple Myeloma, College of Medicine, Inje University) Park, Young Shik (School of Biological Sciences, Inje University) |
1 | Kim, H.-L., Kim, D. H., Lee, Y. K., Park, S. O., Lee, Y.-W., Kwon, O.-S. and Park, Y. S. (2010) An enzymatic method to distinguish tetrahydrobiopterin from oxidized biopterins using UDP-glucose:tetrahydrobiopterin glucosyltransferase. Anal. Biochem. 397, 79-83. DOI ScienceOn |
2 | Lee, Y. G., Kim, A. H., Park, M. B., Kim, H. L., Lee, K. H. and Park, Y. S. (2010) Molecular cloning of cyanobacterial pteridine glycosyltransferases that catalyze the transfer of either glucose or xylose to tetrahydrobiopterin. Appl. Environ. Microbiol. 76, 7658-7661. DOI ScienceOn |
3 | Ruane, K. M, Davies G. J. and Martinez-Fleites C. (2008) Crystal structure of a family GT4 glycosyltransferase from Bacillus anthracis ORF BA1558. Proteins 73, 784-787. DOI ScienceOn |
4 | Martinez-Fleites, C., Proctor, M., Roberts, S., Bolam, D. N., Gilbert, H. J. and Davies, G. J. (2006) Insights into the synthesis of lipopolysaccharide and antibiotics through the structures of two retaining glycosyltransferases from family GT4. Chem. Biol. 13, 1143-1152. DOI ScienceOn |
5 | Arnold, K., Bordoli, L., Kopp, J. and Schwede, T. (2006). The SWISS-MODEL Workspace: A web-based environment for protein structure homology modelling. Bioinformatics 22, 195-201. DOI ScienceOn |
6 | Kabsch, W. and Sander, C. (1983) Dictionary of protein secondary structure: pattern recognition of hydrogenbonded and geometrical features. Biopolymers 22, 2577- 2637. DOI ScienceOn |
7 | Gouet, P., Courcelle, E., Stuart, D. I. and Metoz, F. (1999) ESPript: multiple sequence alignments in PostScript. Bioinformatics 15, 305-308. DOI ScienceOn |
8 | Hansen, E. H., Osmani, S. A., Kristensen, C., Møller, B. L. and Hansen, J. (2009) Substrate specificities of family 1 UGTs gained by domain swapping. Phytochem. 70, 473-482. DOI ScienceOn |
9 | Chung, H. J., Kim, Y.-A., Kim, Y. J., Choi, Y. K., Hwang, Y. K. and Park, Y. S. (2000) Purification and characterization of UDP-glucose: tetrahydrobiopterin glucosyltransferase from Synechococcus sp. PCC 7942. Biochim. Biophys. Acta 1524, 183-188. DOI ScienceOn |
10 | Werner, E. R., Blau, N. and Thöny, B. (2011) Tetrahydrobiopterin: biochemistry and pathophysiology. Biochem. J. 438, 397-414. DOI |
11 | Lairson, L. L., Henrissat, B., Davies, G. J. and Withers, S. G. (2008) Glycosyltransferases: structures, functions, and mechanisms. Annu. Rev. Biochem. 77, 521-555. DOI ScienceOn |
12 | Truman, A. W., Dias, M. V., Wu, S., Blundell, T. L., Huang, F. and Spencer, J. B. (2009) Chimeric glycosyltransferases for the generation of hybrid glycopeptides. Chem. Biol. 16, 676-685. DOI ScienceOn |
13 | Park, S. H., Park, H. Y., Sohng, J. K., Lee, H. C., Liou, K., Yoon, Y. J. and Kim, B. G. (2009) Expanding substrate specificity of GT-B fold glycosyltransferase via domain swapping and high-throughput screening. Biotechnol. Bioeng. 102, 988-994. DOI ScienceOn |
14 | Palcic, M. M. (2011) Glycosyltransferases as biocatalysts. Curr. Opin. Chem. Biol. 15, 226-233. DOI ScienceOn |
15 | Ho, S. N., Hunt, H. D., Horton, R. M., Pullen, J. K. and Pease, L. R. (1989) Site-directed mutagenesis by overlap extension using the polymerase chain reaction. Gene 77, 51-59. DOI ScienceOn |
![]() |