• Title/Summary/Keyword: L2CAP

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Single Nucleotide Polymorphisms linked to the SlMYB12 Gene that Controls Fruit Peel Color in Domesticated Tomatoes (Solanum lycopersicum L.)

  • Kim, Bichsaem;Kim, Nahui;Kang, Jumsoon;Choi, Youngwhan;Sim, Sung-Chur;Min, Sung Ran;Park, Younghoon
    • Horticultural Science & Technology
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    • v.33 no.4
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    • pp.566-574
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    • 2015
  • Yellow or transparent fruit peel color is caused by the accumulation or lack of naringenin chalcone (NG, C) in fruit peel and determines the red or pink appearance of tomato fruit, respectively. NGC biosynthesis is regulated by the SlMYB12 gene of the Y locus on chromosome 1, and DNA markers derived from SlMYB12 would be useful for marker-assisted selection (MAS) of tomato fruit color. To develop a gene-based marker, 4.9 kb of the SlMYB12 gene including a potential promoter region was sequenced from the red-fruited (YY) line 'FCR' and pink-fruited (yy) line 'FCP'. Sequence alignment of these SlMYB12 alleles revealed no sequence variations between 'FCR' and 'FCP'. To identify SlMYB12-linked single nucleotide polymorphisms (SNPs), 'FCR' and 'FCP' were genotyped using a SolCAP Tomato SNP array and CAPS markers (CAPS-456, 531, 13762, and 38123) were developed from the four SNPs (solcap_snp_sl_456, 531, 13762, and 38123) most closely flanking the SlMYB12. These CAPS markers were mapped using $F_2$ plants derived from 'FCR' ${\times}$ 'FCP'. The map positions of the fruit peel color locus (Y) were CAPS-13762 (0 cM) - 456 (11.09 cM) - Y (15.71 cM) - 38123 (17.82 cM) - 531 (30.86 cM), and the DNA sequence of SlMYB12 was physically anchored in the middle of CAPS-456 and CAPS-38123, indicating that fruit peel color in domesticated tomato is controlled by SlMYB12. A total of 64 SolCAP tomato germplasms were evaluated for their fruit peel color and SNPs located between solcap_snp_sl_456 and 38123. Seven SNPs that were detected in this interval were highly conserved for pink-fruited accessions and specific to transparent fruit peel traits, as depicted by a phenetic tree of 64 accessions based on the seven SNPs.

Thermal stability, magnetic and magnetocaloric properties of Gd55Co35M10 (M = Si, Zr and Nb) melt-spun ribbons

  • Jiao, D.L.;Zhong, X.C.;Zhang, H.;Qiu, W.Q.;Liu, Z.W.;Ramanujan, R.V.
    • Current Applied Physics
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    • v.18 no.12
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    • pp.1523-1527
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    • 2018
  • The thermal stability, magnetic and magnetocaloric properties of $Gd_{55}Co_{35}M_{10}$ (M = Si, Zr and Nb) melts-pun ribbons were studied. The relatively high reduced glass transition temperature ($T_{x1}/T_m$ > 0.60) and low melting point ($T_m$) resulted in excellent glass forming ability (GFA). The Curie temperatures ($T_C$) of melt-spun amorphous ribbons $Gd_{55}Co_{35}M_{10}$ for M = Si, Zr and Nb were 166, 148 and 173 K, respectively. For a magnetic field change of 2 T, the values of maximum magnetic entropy change $(-{\Delta}S_M)^{max}$ for $Gd_{55}Co_{35}Si_{10}$, $Gd_{55}Co_{35}Zr_{10}$ and $Gd_{55}Co_{35}Nb_{10}$ were found to be 2.86, 4.28 and $4.05J\;kg^{-1}K^{-1}$, while the refrigeration capacity (RC) values were 154, 274 and $174J\;kg^{-1}$, respectively. The $RC_{FWHM}$ values of amorphous alloys $Gd_{55}Co_{35}M_{10}$ (M = Si, Zr and Nb) are comparable to or larger than that of $LaFe_{11.6}Si_{1.4}$ crystalline alloy. Large values of $(-{\Delta}S_M)^{max}$ and RC along with good thermal stability make $Gd_{55}Co_{35}M_{10}$ (M = Si, Zr and Nb) amorphous alloys be potential materials for magnetic cooling operating in a wide temperature range from 150 to 175 K, e.g., as part of a gas liquefaction process.

Studoes on the Slurry-Application of Winter Rye (Secale cereale L. ) II. Effect of mineral content , nitrogen balance and environmental soil (추파용 호밀에 대한 액상분뇨 시비 연구 II. 무기물 함량 , N 생산성 및 토양환경에 미치는 영향)

  • 신동은;김동암;신재순;송관철;이종경;윤세형;김원호;김정갑
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.18 no.3
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    • pp.243-250
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    • 1998
  • This study was conducted to evaluate mineral content of Winter Rye, Nitrogen Balance, Soil chemical characteristics and NO,-N level in infiltration water as affected by different slurry application on the basis of N contents respectively and was arranged as a randomized complete block design with seven treatments (chemical fertilizer 160kg Nha, cattle slurry 160 . 320 - 480kg Nha, swine slurry 160 - 320 - 480kg Nha) and conducted at National Livestock Research Institute, RDA, in Suweon fiom Sep. 1996. to Apr. 1997. The results obtained are summarized as follows : Nirogen, Phosphorous, Calcium and Potassium of Winter Rye slightly increased as slurry application level was increased (p$NO_3$-N content ($\mu$g/g) of Winter Rye was shown fiom 780 to 1,462 in all treatments, especially , the swine slurry plots were significantly higher than that of cattle sluny plots (p < 0.05). As slurry application increased, show a marked trend toward surplus N input in all treatment. The pH of the topsoil (0~IOcm) was shown 6om 5.1 to 5.4 except the plot of chemical fertilizer before experiment pH of 5.1, but the pH of the subsoil (10~20cm) tended not to be regular. The content of available ,TEX>$P_2O_5$ inaeased with the increased application of slurry, particularly, the topsoil (O- 1Ocm) were much higher than the subsoil (10~20cm). The $NO_3$-N content of infiltration water (depth:lm) was increased as affected by sluny application.

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Identification of Lacrymaria velutina (Pers. Ex Fr.) Konrad & Maubl. from Micheon-myeon, Jinju-city, Korea

  • Han, Ki-Soo;Volk, Thomas J.;Kim, Hee-Kyu
    • Mycobiology
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    • v.38 no.4
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    • pp.249-255
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    • 2010
  • We identified Lacrymaria velutina of the Coprinaceae in Korea. The unusually large and sturdy fruiting body, fibrillose to fibrillose-scaly cap and stalk without a volva with an obscure superior hairy ring zone or hairy annulus, and blackish brown, warted spores distinguished this species from closely related Psathyrella species. An illustrated account of the microscopic traits is presented. Fruiting bodies with obtusely hemispherical caps, 2.5~6 cm, becoming convex with age; surface dry, densely fibrillose-scaly with split margin; stipe, 4.5~6 cm, equal, hollow, fibrillose, dry, whitish above the superior ring zone, light brown below; crowded gills, adnexed, dark black at maturity. Pileipellis typically cellular with the gill edge appearing white and beaded. Blackish brown basidiospores that discolor in concentrated sulfuric acid. Spores elliptical, warted, $9\sim11{\times}6\sim8{\mu}m$, with prominent snout-like germpores. Cheilocystidia abundant, $57\sim68{\times}19\sim25{\mu}m$, and narrowly elongated clavate, often clustered in threes or fours. Pleurocystidia rarely present, $45\sim47.5{\times}12\sim13{\mu}m$, and clavate to utriform. This trait distinguishes our sample as L. velutina from other Psathyrella spp. of the Coprinaceae, which have smooth spores. This taxon was clarified by the observation that Psathyrella spores fade in concentrated sulfuric acid. A molecular phylogenetic study revealed that our specimen was Lacrymria velutipes, which is closely related to Lacrymaria lacrymabunda. Moreover, those two species are clearly distinguishable from other Psathyrella species, which agreed with the morphologically distinctive traits described above. We believe that this is the first report of this taxon, which has not been described in Korea.

Mechanistic investigations on emission characteristics from g-C3N4, gC3N4@Pt and g-C3N4@Ag nanostructures using X-ray absorption spectroscopy

  • Sharma, Aditya;Varshney, Mayora;Chae, Keun Hwa;Won, Sung Ok
    • Current Applied Physics
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    • v.18 no.11
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    • pp.1458-1464
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    • 2018
  • An improved method for the preparation of g-$C_3N_4$ is described. Currently, heating (> $400^{\circ}C$) of urea is the common method used for preparing the g-$C_3N_4$. We have found that sonication of melamine in $HNO_3$ solution, followed by washing with anhydrous ethanol, not only reduce the crystallite size of g-$C_3N_4$ but also facilitate intriguing electronic structure and photoluminescence (PL) properties. Moreover, loading of metal (Pt and Ag) nanoparticles, by applying the borohydride reduction method, has resulted in multicolor-emission from g-$C_3N_4$. With the help of PL spectra and local electronic structure study, at C K-edge, N K-edge, Pt L-edge and Ag K-edge by X-ray absorption spectroscopy (XAS), a precise mechanism of tunable luminescence is established. The PL mechanism ascribes the amendments in the transitions, via defect and/or metal states assimilation, between the ${\pi}^*$ states of tris-triazine ring of g-$C_3N_4$ and lone pair states of nitride. It is evidenced that interaction between the C/N 2p and metal 4d/5d orbitals of Ag/Pt has manifested a net detraction in the ${\delta}^*{\rightarrow}LP$ transitions and enhancement in the ${\pi}^*{\rightarrow}LP$ and ${\pi}^*{\rightarrow}{\pi}$ transitions, leading to broad PL spectra from g-$C_3N_4$ organic semiconductor compound.

Varietal Responses of Soybean Germination and Seedling Elongation to Temperature and Polyethylene Glycol Solution (온도와 PEG에 대한 대두품종의 발아 및 묘신장 반응)

  • Seong, Rak-Chun;Chung, Hae-Joon;Hong, Eun-Hi
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.33 no.1
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    • pp.31-37
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    • 1988
  • Germination and seedling elongation of soybeans [Glycine max. (L.) Merr., cults. Bangsakong, Hill, Paldalkong, Danyupkong, Baegwun-kong. Kwangkyokong, Changyupkong and Hwangkeumkong] were measured at two temperatures (15 and 30$^{\circ}C$) and four polyethylene glycol 10,000 (PEG) solutions (0, 20, 30 and 35g/100g-H$_2$O). Adjustments of PEG solution were based on water potentials of 0.0, -0.5, -1.1 and -1.5 MPa at room temperature. Observations were made at 3, 6, 9 and 12 days for 15$^{\circ}C$ and 1, 2, 3 and 4 days for 30$^{\circ}C$. Fifteen seeds of each cultivar were placed on Whatman No. 1 (9cm) filter paper in plastic pertridishes, and adjusted to 15 ml of the proper PEG solution supplemented with 0.2 percent thiram using automatic syringe. The dishes were covered with cap. The seeds were germinated at a continuous temperature of 15 or 30$^{\circ}C$ under dark conditions for programmed period. Seedling moisture content and seedling length of eight soybean cultivars decreased as PEG concentration increased both at 15 and 30$^{\circ}C$. Cultivar differences in seedling moisture content and seedling length were found among eight soybean cultivars at temperatures of 15 and 30$^{\circ}C$. Larger sized cultivar absorbed more moisture than samller sized cultivar. However, reverse results were obtained on the seedling moisture content of each of eight soybean cultivars. Cultiver Hill and Paldalkong showed greater seedling length than the other six cultivars from 20 to 30g/100g water of PEG concentrations both at 15 and 30$^{\circ}C$. The results of this study indicated that germination test of soybean seeds in aqueous solutions of PEG has potential for screening soybean cultivars for improved emergence during moisture stress.

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Autotrophic Growth of Limonium spp. 'Ocean Blue' Plantlets In Vitro as Affected by PPF, NAEH and $\textrm{CO}_2$ Concentration (스타티스 ‘오션 블루’의 자가영양배양시 광도, 환기횟수 및 $\textrm{CO}_2$ 농도가 소식물체의 기내 생육에 미치는 영향)

  • 정기원;정병룡
    • Journal of Bio-Environment Control
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    • v.11 no.3
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    • pp.115-120
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    • 2002
  • Growth and development of Limonium spp.‘Ocean Blue’plantlets were studied under three levels of photosynthetic photon flux (PPF),70,150 and 220 $\mu$mol. $m^{-2}$ . $s^{-1}$ , two levels of $CO_2$ concentration, 500 and 1000 $\mu$mol. $m^{-1}$ , and two levels of number of air exchanges per hour (NAEH),0.1 $h^{-1}$ and 2.8 $h^{-l}$. Explants were obtained from photomixotrophically-micropropagated plantlets. Four explants per vessel were cultured under cool-white fluorescent lamps for 16 h. $d^{-1}$ at 25$\pm$11$^{\circ}C$ and 70~80% relative humidity. In treatments of 2.8 $h^{-1}$ NAEH, a 10 mm round hole made on the vessel cap was sealed with a microporous filter and two $CO_2$ concentrations in the culture rooms were provided from a liquefied $CO_2$ tank. Fresh and dry weights, height, length of the longest root, number of loaves, and leaf area significantly increased with increasing PPF and especially, $CO_2$ concentration. Growth was enhanced by a 2.8 $h^{-1}$ NAEH. Overall, treatment with a 220 $\mu$mol. $m^{-2}$ . $s^{-1}$ PPF and a 1000 $\mu$mol. $m^{-1}$ $CO_2$ resulted in the most vigorous growth of Limonium spp. ‘Ocean Blue’ plantlets.s.

Characteristics and breeding of a new variety Pleurotus eryngii, Gongi No.3 (신품종 큰느타리버섯 '곤지3호' 육성 및 특성)

  • Ha, Tai-Moon;Ju, Young-Cheol;Jeon, Dae-Hoon;Choi, Jong-In;Lee, Tae-Soo
    • Journal of Mushroom
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    • v.9 no.1
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    • pp.22-26
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    • 2011
  • We bred a new strain of Pleurotus eryngii. It's name is 'Gongi No.3' and it was bred by mating monokaryotic strain isolated from E08-5D2 and dikaryotic strain GMPE25016 from 2006 to 2010 in Mushroom Research Station, Gyonggi province A.R.E.S. The characteristics of a new strain 'Gongi No.3' is as follows ; The optimum temperature for mycelium growth was from 26 to 29 degrees celsius on PDA medium and those for the premodium formation and the growth of fruit body were from 14 to 18 degrees celsius. The period of spawn running was around 30days at 22 degrees celsius and the period taken from scratching old spawn to make premodium were 8 days. The color degree of cap surface was measured by color difference meter and that of a new strain 'Gongi No.3' was 54.4 by L-value. it was seem to be dark, compared with 'Keunneutari No.2'. The hardness of fruit body of a new strain was higher than 'Keunneutari No.2'. The yield was about 180g per bottle(1100cc). it was 10g more than 'Keunneutari No.2'.

Effect of $CO_2$ Concentration, NAEH and Light Intensity on the Photoautotrophic Growth of Campanula punctata 'Rubriflora' Plantlets In Vitro (자주초롱꽃의 기내 자가영양배양시 $CO_2$농도, 환기횟수 및 광도가 생장에 미치는 영향)

  • Shim, Jae-Nam;Kim, Gyeong-Hee;Jeong, Byoung-Ryong
    • Journal of Bio-Environment Control
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    • v.14 no.4
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    • pp.233-238
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    • 2005
  • Growth of Campanula punctata 'Rubriflora' plantlets, as affected by three levels of photosynthetic photon flux (PPF), 70, 110, and $220{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$, two levels of $CO_2$ concentration, 500 and $1,500{\mu}mol{\cdot}m^{-1}$, and two levels of number of air exchanges per hour (NAEH), 0.1 and $2.8 h^{-l}$, was studied. Explants were obtained from photomixotrophically-micropropagated plantlets. Four explants were planted in each $3.7{\times}10^{-4}m^3$ polycarbonate box containing MS basal medium and no added sucrose. Explants were cultured under cool-white fluorescent lamps for $16h{\cdot}d^{-1},\;at\;25\pm1^{\circ}C$ temperature, and $70\~80\%$ relative humidity In treatments of $2.8h^{-1}$ NAEH, a 10mm round hole made on the vessel cap was sealed with a microporous filter. For higher $CO_2$ concentrations in the culture room, $CO_2$ gas was provided from a tank of liquefied $CO_2$. Fresh and dry weights, length of the longest root, and number of leaves significantly increased with increasing PPF and especially $CO_2$ concentration. Length of the longest root, number of leaves, fresh and dry weights, and chlorophyll concentration were enhanced with increased NAEH. However, leaf area was the smallest in the $220{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}\;PPF\;2.8h^{-1}$ NAEH and especially, $1,500{\mu}mol{\cdot}mol^{-1}\;CO_2$ concentration treatment. Treatment effect became more produced with time. Overall, treatment with $220{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}\;PPF\;and\;1,500{\mu}mol{\cdot}mol^{-1}\;CO_2$ gave the most vigorous growth.

Estimation of Pollutant Load Delivery Ratio for Flow Duration Using L-Q Equation from the Oenam-cheon watershed in Juam Lake (유량-부하량관계식을 이용한 주암호 외남천 유역의 유황별 유달율 산정)

  • Choi, Dong-Ho;Jung, Jae-Woon;Lee, Kyoung-Sook;Choi, Yu-Jin;Yoon, Kwang-Sik;Cho, So-Hyun;Park, Ha-Na;Lim, Byung-Jin;Chang, Nam-Ik
    • Journal of Environmental Science International
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    • v.21 no.1
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    • pp.31-39
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    • 2012
  • The objective of this study is to provide pollutant loads delivery ratio for flow duration in Oenam-cheon watershed, which is upstream watershed of Juam Lake. To calculate the delivery ratio by flow duration, rating curves and discharge-loads curves using measured data were established, then Flow Duration Curve(FDC) and pollutant loads delivery ratio curves were constructed. The results show that the delivery ratios for $BOD_5$ for abundant flow($Q_{95}$), ordinary flow($Q_{185}$), low flow($Q_{275}$), and drought flow($Q_{355}$) were 23.9, 12.7, 7.1, and 2.9%, respectively. The delivery ratios of same flow regime for T-N were 58.4, 31.2, 17.2 and 7.1%, respectively. While, the delivery ratios T-P were 17.3, 7.5, 3.4, and 1.1% respectively. In general, delivery ratio of high flow condition showed higher value due to the influence of nonpoint source pollution. Based on the study results, generalized equations were developed for delivery ratio and discharge per unit area, which could be used for ungaged watershed with similar pollution sources.