Abstract
Growth of Campanula punctata 'Rubriflora' plantlets, as affected by three levels of photosynthetic photon flux (PPF), 70, 110, and $220{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$, two levels of $CO_2$ concentration, 500 and $1,500{\mu}mol{\cdot}m^{-1}$, and two levels of number of air exchanges per hour (NAEH), 0.1 and $2.8 h^{-l}$, was studied. Explants were obtained from photomixotrophically-micropropagated plantlets. Four explants were planted in each $3.7{\times}10^{-4}m^3$ polycarbonate box containing MS basal medium and no added sucrose. Explants were cultured under cool-white fluorescent lamps for $16h{\cdot}d^{-1},\;at\;25\pm1^{\circ}C$ temperature, and $70\~80\%$ relative humidity In treatments of $2.8h^{-1}$ NAEH, a 10mm round hole made on the vessel cap was sealed with a microporous filter. For higher $CO_2$ concentrations in the culture room, $CO_2$ gas was provided from a tank of liquefied $CO_2$. Fresh and dry weights, length of the longest root, and number of leaves significantly increased with increasing PPF and especially $CO_2$ concentration. Length of the longest root, number of leaves, fresh and dry weights, and chlorophyll concentration were enhanced with increased NAEH. However, leaf area was the smallest in the $220{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}\;PPF\;2.8h^{-1}$ NAEH and especially, $1,500{\mu}mol{\cdot}mol^{-1}\;CO_2$ concentration treatment. Treatment effect became more produced with time. Overall, treatment with $220{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}\;PPF\;and\;1,500{\mu}mol{\cdot}mol^{-1}\;CO_2$ gave the most vigorous growth.
자주초롱꽃(Campanula punctata 'Rubriflora')의 자가영양배양시 $CO_2$ 농도,환기횟수 및 광도가 소식물체의 생장에 미치는 영향을 조사하였다. $1,500{\mu}mol{\cdot}m^{-1}$고농도$CO_2$에서는 광도를 높일수록 초장, 엽수가 증가되었으나, 최대근장은 유의성이 인정되지 않았다 엽록소의 함량은 광도가 높을 때보다는 낮은 경우인 $70{\mu}mol{\cdot}m^{-2}s^{-1}$에서 가장 많이 증가되었다 엽면적은 광도가 높을수록 작아지는 경향을 보였으며, $CO_2\;1,500{\mu}mol{\cdot}mol^{-1}$ 환기횟수 $2.8h^{-l},\;PPF\; 220{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$에서 가장 큰 차이로 작게 나타났다. 생체중과 건물중은 $CO_2$ 농도, 환기횟수, PPF가 높을수록 증가되었다. 특히 $CO_2\;1,500{\mu}mol{\cdot}mol^{-1}$, 환기횟수 $2.8h^{-1},\;PPF\; 220{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$에서 가장 크게 증가되었다. 환기횟수의 영향은 전반적으로 적었다. 종합적으로 자주초롱꽃 소식물체의 기내 자가영양배양 차량번식을 위해서는 $CO_2\;1,500{\mu}mol{\cdot}mol^{-1},\;PPF\;220{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$의 조건이 적합하였다.
