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Autotrophic Growth of Limonium spp. 'Ocean Blue' Plantlets In Vitro as Affected by PPF, NAEH and $\textrm{CO}_2$ Concentration  

정기원 (경상대학교 응용생명과학부)
정병룡 (경상대학교 농업생명과학연구원)
Publication Information
Journal of Bio-Environment Control / v.11, no.3, 2002 , pp. 115-120 More about this Journal
Abstract
Growth and development of Limonium spp.‘Ocean Blue’plantlets were studied under three levels of photosynthetic photon flux (PPF),70,150 and 220 $\mu$mol. $m^{-2}$ . $s^{-1}$ , two levels of $CO_2$ concentration, 500 and 1000 $\mu$mol. $m^{-1}$ , and two levels of number of air exchanges per hour (NAEH),0.1 $h^{-1}$ and 2.8 $h^{-l}$. Explants were obtained from photomixotrophically-micropropagated plantlets. Four explants per vessel were cultured under cool-white fluorescent lamps for 16 h. $d^{-1}$ at 25$\pm$11$^{\circ}C$ and 70~80% relative humidity. In treatments of 2.8 $h^{-1}$ NAEH, a 10 mm round hole made on the vessel cap was sealed with a microporous filter and two $CO_2$ concentrations in the culture rooms were provided from a liquefied $CO_2$ tank. Fresh and dry weights, height, length of the longest root, number of loaves, and leaf area significantly increased with increasing PPF and especially, $CO_2$ concentration. Growth was enhanced by a 2.8 $h^{-1}$ NAEH. Overall, treatment with a 220 $\mu$mol. $m^{-2}$ . $s^{-1}$ PPF and a 1000 $\mu$mol. $m^{-1}$ $CO_2$ resulted in the most vigorous growth of Limonium spp. ‘Ocean Blue’ plantlets.s.
Keywords
culture environment; light intensity; micropropagation; ventilation;
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1 Kozai, T. and Y. Iwanami. 1988. Effects of $CO_2$ enrich-ment and sucrose concentration under high photon fluxes on plantlet growth of carnation (Dianthus caryophylIus L.) in tissue culture during the propaga-tion stage. J. Japan. Soc. Hort. Sci. 57:279-288   DOI
2 Kozai, T., S. Kushihashi, C. Kubota, and K. Fujiwara, 1992a. Effect of the difference in air temperature between photoperiod and dark period on morphogene-sis and growth of potato plantlets in vitro under photo-autotrophic and $CO_2$ enriched conditions. J. Japan. oc. Hort. Sci. 61:93-98   DOI
3 Desjardins, Y., A. Gosselin, and M. Lamarre. 1990. Growth of transplants and in vitro-cultured clones of asparagus in response to $CO_2$ enrichment and supple-mental lighting. J. Amer. Soc. Hort. Sci. 115:364-368
4 정병룡, 이은주, 김수정,황승재. 1996. 발근단계에서의 기내 영양체계와 기외 순화방법이 카네이션과 딸기 배양묘의 생육에 미치는 영향. 한국식물조직배양학회 발표요지. pp. 37-38
5 이용범, 이병일. 1994. $CO_2$ 장기 시용이 토마토의엽온, 확산저항 및 광합성에 미치는 영향. J. Kor. Soc. Hort. Sci. 35:421-428
6 이은주. 1998. Study of the Autotrophic Culture of Limomnium spp. cv. Misty Blue. 경상대학교. 석사학위논문. p. 26-27
7 Fujiwara, K., T. Kozai, and I. Watanabe. 1987. Funda-mental studies on environments in plant tissue culture vessels. J. Agr. Meteorol. 43:21-30   DOI
8 Coumac, L., I. Cirier, and P. Chagvardieff. 1992. Improvement of photoautotrophic Solanum tuberosum plantlet culture by light and $CO_2$: Differential develop-ment of photosynthetic characteristics and varietal con-straints. Acta Hort. 319:53-58
9 Fujiwara, K., S. Kira, and T. Kozai. 1992. Time course of $CO_2$ exchange of potato cultures in vitro with differ-ent sucrose concentrations in the culture medium. J. Agr. Meteorol. 48:49-56   DOI
10 Kirdmanee, C., Y. Kitaya, and T. Kozai. 1995b. Effects of $CO_2$ enrichment and supporting material on growth,photosynthesis and water potential of Euclyptus shoots/plantlets cultured photoautotrophically in vitro. Environ Control Biol. 33:133-141   DOI
11 Murashige, T. and F. Skoog. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15:473-497   DOI
12 Grout, B.W. and S. Millam. 1985. Photosynthetic development of micropropagated strawberry plantlets following transplanting. Ann. Bot. 55:129-131   DOI
13 Kozai, T., K. Fujiwara, M. Hauashi, and J. Aitken-Christe, 1992b. The in vitro environment and its Con-trol in micropropagation. pp. 247-282. In: Transplant Production System (K. Kurata and T. Kozai, Eds). Kluwer Academic Publishers
14 Kozai, T. and K. Sekimoto, 1988. Effects of the num-ber of air changes per hour of the closed vessel and the photosynthetic photon flux on the carbon dioxide con-centration inside the vessel and the growth of straw-berry plantlets in vitro. Environ. Control Biol. 26:21-29   DOI
15 Kim, J.S. and B.R. Jeong. 2001. Growth, Photosyn-thetic rate and stomatal function of Spathiphyllum in vitro as affected by PPF and NAEH. J. Kor. Hort. Sci. 42:351-352
16 Desjardins, Y., F. Laforge, C. Lussier, and A. Gosse-lin. 1988. Effect of $CO_2$ enrichment and high photo-synthetic photon flux on the development of autotro-phy and growth of tissue cultured strawberry, rasp-berry and asparagus plants. Acta Hort. 230:45-53
17 Desjardins, Y., A. Gosselin, and S. Yelle. 1987. Accli-matization of in vitro strawberry plantlets in $CO_2$enriched environments and supplemental lighting. J. Amer. Soc. Hort. Sci. 112:846-851
18 Kirdmanee, C., Y. Kitaya, and T. Kozai. 1995a. Effects of $CO_2$ enrichment and supporting material in vitro onphotoautotrophic growth of Eucalyptus plantlets in vitro and ex vitro: Anatomical comparisons. Acta Hort. 393:111-118
19 Jeong, B.R., K. Fujiwara, and T. Kozai. 1995. Carbon dioxide enrichment in autotrophic micropropagation:Methods and Advantages. HortTechnology 3:332-334