• Food Science of Animal Resources
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• v.33 no.3
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• pp.403-410
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• 2013

This study was performed to evaluate the microbiological contamination level of raw beef from retail markets in Seoul, Korea. The sampling and laboratory test were performed according to the procedure of "Standard for processing and ingredients specification of livestock product" and "Korean food code". Enterotoxin of Staphylococcus aureus isolates were detected using VIDAS$^{(R)}$ and PCR-based methods. Listeria monocytogenes serotyping and genotyping were carried out using Listeria antisera and L. monocytogenes Fingerprinting kit, respectively. A total of 48 samples were collected from 16 retail markets (butcher's shop: 5, department store: 6, supermarket: 5) in 2011. The level of total bacteria counts in the butcher's shop, department store and supermarket were $4.4{\times}10^3$ CFU/g, $3.9{\times}10^5$ CFU/g and $1.0{\times}10^4$ CFU/g, respectively. The concentrations of Escherichia coli of these three retail markets were $6.4{\times}10$ CFU/g, 7.6 CFU/g and $2.0{\times}10$ CFU/g, respectively. Salmonella species was not detected on all samples. However, S. aureus was isolated in the 3 samples (6.25%) from each type of three retail markets. L. monocytogenes was isolated in the 4 samples (8.3%) from department stores. The level of contamination of these foodborne bacteria was less than 100 CFU/g. The enterotoxin-encoding genes of S. aureus isolates were sea, seh, sei and sep gene. The gene similarity of L. monocytogenes isolated from two retail markets by Rep-PCR showed 57.8-98.1% and 68.1-98.1%, respectively. These results suggest that the HACCP guideline for environmental control in slaughterhouse and retail markets should be provided to prevent cross contamination and manage foodborne pathogens such as L. monocytogenes and S. aureus.

• Journal of Life Science
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• v.11 no.2
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• pp.103-110
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• 2001

This study was designed to investigate the effects of silkworm(Bombyx moril L.) powder on oxidative stress and membrane fluidity in brain membranes of rats. Sprague-Dawley(SD) male rats(160$\pm$10 g) were fed basic diet(control group), and experimental diets(SWP-200 and SWP-400 groups) added 200 and 400mg/kg BW/day for 6 weeks. There were no significant differences in cholesterol levels of brain memberanes by administration of silkworm powder (SWP). Membrane fluidities were significantly increased(21.5% and 30.8%, respectively) in brain mitochondria of SWP-200 and SWP-400 groups compared with control group, but significant difference between brain microsomes could not obtained. Basal oxygen radicals (BORs) in brain mitochondria and mircrosomes were significantly inhibited(8.5% and 16.5%, 16.8%and 24.8%, respectively) by SWP-200 and SEP-400 groups compared with control group. Induced oxygen radicals(IORs) in brain mitochondria were significantly inhibited(16.6% and 21.4%, respectively)by sWP-200 and SWP-400 groups compared with control group, but IOR in brain microsome were significantly inhibited about 16.0% by SWP-400 groups only compared with control group. Lipid peroxide(LPO) levels were significantly decreaed(14.8%and 22.4%, respectively) in brain mitochondria of SWP-200 and SWP-400 groups compared with control group, but LPO level was significantly decreased about 16.0% in brain microsome of SWP-400 group only. Oxidized protein(OP) levels were remarkably decreased(about 14.8% and 16.5%, respectively) in brain mitochondria of SWP-200 and SWP-400 groups, but OP level was significantly decreased about 13.0% in brain microsome of SWP-400 group only compared with control group, Theses results suggest that administration of in brain microsome of SWP-400 group only compared with control group. These results suggest that administration of SWP may play effective role in attenuating an oxidative stress and increasing a membrane fluidity in brain membranes.

• Journal of The Korean Society of Grassland and Forage Science
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• v.14 no.3
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• pp.215-222
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• 1994

This experiment was conducted to find out the response of orchardgrass (Dactylis glorneruta L.) varieties to grazing at the condition of monocultures and mixed pastures on the field of Livestock Experiment Station at Suwon from Sep. 1989 to Oct. 1991. The varieties of orchardgrass used in this experiment were Potomac, Hallmark and Syn. No. 2 as a early maturing type, and Lidacta, Rancho and Syn. No. 10 as a late maturing type. The results obtained were as follows: 1. Herbage utilization of mixture with Hallmark variety(T2) was the highest as 78.2% and that with Rancho variety(T5) was the lowest as 70.2%. but there was no difference(P>0.05). Mixtures with early maturing varieties(T1, T2 and T3) were higher as 77.4% than those with late varieties(T4, T5 and T6) as 72.9% in herbage utilization. In monocultures Rancho variety(T5) was the highest as 68.6% and Lidacta variety(T4) was the lowest as 58.8%, and mixtures with early maturing varieties(T1, T2 and T3) as 66.6% were higher than those of late(T1, 72 and 73) as 64.6%. 2. The content of crude protein of 6 mixed pastures ranged from 19.7% to 22.8%, and those of ether extracts, crude fiber, crude ash and nitrogen free extract were 6.6 to 7.5%, 24.9 to 26.2'76, 1 1.5 to 1 1.9% and 33.6-35.4 8, respectively, But there were not different significantly(P>0.05). In monocultures, significant difference by variety was observed in crude protein content(P<0.05). 3. In botanical composition, there was just a little different among 6 mixtures, having 90% or more in grass percentage. The percentages of each orchardgrass varieties were decreased, but those of perennial ryegrasses were increased as grazing sesson was progressed in all treatment.

• Journal of The Korean Society of Grassland and Forage Science
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• v.14 no.3
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• pp.206-214
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• 1994

This experiment was conducted to find out the response of orchardgrass (Dactylis glomrrata L.) varieties to grazing at the condition of monocultures and mixed pastures on the field of Livestock Experiment Station at Suwon from Sep. 1989 to Oct. 1991. The varieties of orchardgrass used in this experiment were Potomac, Hallmark and Syn. No. 2 as a early maturing type, and Lidacta, Rancho and Syn. No. 10 as a late maturing type. The results obtained were as follows: I. Heading date of early maturing varieties among orchardgrass like as Potomac, Hallmark and Syn. 2 ranged from May 9 to May 14. Otherwise those of late varieties(Lidacta Rancho and Syn. 10) were between May 21 to May 25. Leaf types of early varieties were shown erect, those of late varieties were erect or semi-erect. 2. In dry matter production per ha, mixture with Rancho variety(T5) was the highest as 7.818 kg and mixture with Hallmark(T2) was the lowest as 7,013 kg. But there was not significantly different(P>0.05) among mixtures. While in monocultures, Syn. 10 variety was the highest(6.626 kglha) and Lidacta was Iowest(5,775 kg). But no difference was found(P>0.05). 3. In mean grass height after grazing, mixtures with early maturing types were higher as 9.8 cm than that of late (8.8cm), and early types were higher as 10.5cm than that of late(9.4cm) in monocultures. But there was no significant difference both mixtures and monocultures.

• Journal of Bio-Environment Control
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• v.20 no.4
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• pp.333-340
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• 2011

This study was conducted on the effect of plant growth retardants (PGRs) on growth of potted Ardisia pusilla and Ardisia pusilla var. variegata grown for a short term in an ebb and flow system. Plants were planted in 10 cm plastic pots, containing a mixture of peat moss and perlite (1 : 1, v/v) on 30 June 2005 and were grown until 23 Sep. 2005. The PGRs used were 50, 200, 350, 500 $mg{\cdot}L^{-1}$ daminozide (B- 9), 10, 40, 70, 100 $mg{\cdot}L^{-1}$ paclobutrazol (Boundy), and 5, 15, 25, 35 $mg{\cdot}L^{-1}$ ethephon (Florel). On every irrigation, the nutrient solution containing PGRs was supplied at a 2 cm depth and was kept for 15~20 minutes. The surplus nutrient solution was drained back into the tank for next irrigations. In both cultivars, paclobutrazol gave the most pronounced effect in inhibition of stretchiness. Ardisia pusilla, which was pinched just before initiation of paclobutrazol treatment, resulted in the greatest inhibition of shoot elongation. Daminozide treatments above 200 $mg{\cdot}L^{-1}$ also gave reduction of shoot length. However, ethephon treatments gave no dwarfing effect in Ardisia pusilla. Inhibition of stretchiness was observed even in the lowest concentration treatments. Among the PGRs, paclobutrazol was the most effective in suppressing plant stretchiness.

• Korean Journal of Food Science and Technology
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• v.33 no.1
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• pp.33-39
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• 2001

To develop a method for separation process using Sep-pak $C_18$, simultaneous and systematic analysis of 8 permitted and 11 non-permitted synthetic food colors in Korea, optimization of analysis conditions for reverse phase ion-pair high performance liquid chromatography was carried out. For the best result of Sep-pak $C_18$ separation the pH of color standard mixture solution was $5{\sim}6$ and 0.1% HCl-methanol solution were set as eluent. The colors eluated from Sep-pak $C_18$ cartridge were determined and confirmed by high performance liquid chromatography with a photodiode array detector at 420 nm for yellow colors type, at 520 nm for red colors type, at 600 nm for blue and green colors type and at 254 nm for mixed colors. Conditions for HPLC analysis were as follows: column, Symmetry $C_18$ (5 m, 3.9 mm $i.d.{\times}150\;mm$); mobile phase, 0.025 M ammonium acetate (containing 0.01 M tetrabutylammonium bromide) : acetonitrile : methanol (65 : 25 : 10) and 0.025 M ammonium acetate(containing 0.01 M tetrabutylammonium bromide) : acetonitrile : methanol (40 : 50 : 10); flow rate, 1 mL/min. It takes 35 minutes for simultaneaus analysis and 18 minutes for systematic analysis. The detection limits range of each colors were $0.01{\sim}0.05\;{\mu}g/g$.

• Journal of The Korean Society of Grassland and Forage Science
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• v.26 no.1
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• pp.53-62
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• 2006

Near infrared reflectance spectroscopy (NIRS) has been increasingly used as a rapid, accurate method of evaluating some chemical compositions in forages. Analysis of forage quality by NIRS usually involves dry ground samples. Costs might be reduced if samples could be analyzed without drying or grinding. The objective of this study was to investigate effect of sample preparations and spectral math treatments on prediction ability of chemical composition for corn silage by NIRS. A population of 112 corn silage representing a wide range in chemical parameters were used in this investigation. Samples of com silage were scanned at 2nm intervals over the wavelength range 400-2500nm and the optical data recorded as log l/Reflectance(log l/R) and scanned in overt-dried grinding(ODG), liquid nitrogen grinding(LNG) or intact fresh(IF) condition. Samples were analysed for neutral detergent fiber(NDF), acid detergent fiber(ADF), acid detergent lignin(ADL), crude protein(CP) and crude ash content were expressed on a dry-matter(DM) basis. The spectral data were regressed against a range of chemical parameters using modified partial least squares(MPLS) multivariate analysis in conjunction with four spectral math treatments to reduce the effect of extraneous noise. The optimum calibrations were selected on the basis of minimizing the standard error of cross validation(SECV). The results of this study show that NIRS predicted the chemical parameters with very high degree of accuracy(the correlation coefficient of cross validation$(R^2cv)$ range from $0.70{\sim}0.95$) in ODG. The optimum equations were selected on the basis of minimizing the standard error of prediction(SEP). The Optimum sample preparation methods and spectral math treatment were for ADF, the ODG method using 2,10,5 math treatment(SEP = 0.99, $R^2v=0.93$), and for CP, the ODG method using 1,4,4 math treatment(SEP = 0.29. $R^2v=0.91$).

• Korean Journal of Food Science and Technology
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• v.31 no.6
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• pp.1471-1476
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• 1999

A simple and practical method for determination of caffeine in foods was developed. The analysis of caffeine was performed by reverse phase high performance liquid chromatography using a ${\mu}-Bondapak\;C_{18}$ column at isocratic condition with methanol-acetic acid-water(20 : 1 : 79) on UV detector at 280 nm. The clean-up and extraction of caffeine in samples were based on a simple pretreatment using a Sep-Pak $C_{18}$ cartridge. Recovery rates obtained with this method for cider, candy, cookie, milk, ice cream and persimmon leaf tea were 99.23%, 99.50%, 99.17%, 99.37%, 98.93% and 99.10% respectively. And the detection limit of caffeine was $0.1\;{\mu}g/mL$. With this method, the range of caffeine contents extracted from coffee, green tea, black tea, Oolong tea(tea bag), soft drinks, ice cream, milk and commercial confectionery were $3.38{\sim}37.50\;mg/g,\;16.30{\sim}26.10\;mg/g,\;10.80{\sim}16.65\;mg/g,\;11.25\;mg/g,\;0.06{\sim}0.11\;mg/g,\;0.04{\sim}0.44\;mg/g,\;0.04{\sim}0.39\;mg/g\;and\;0.10{\sim}1.80\;mg/g$, respectively. But caffeine was not detected in the other tea such as Acanthopanax sessiliflorum tea, Angelica gigas tea, Angelica tea, Arrow root tea, Duchu'ng tea, Dunggulle tea, Ganoerma lucidum tea, Ginger tea powder, Persimmon leaf tea, Ssanghwa tea and Cocoa mix powder.

• Korean Journal of Optics and Photonics
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• v.19 no.6
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• pp.416-421
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• 2008

A scheme for predicting the concentration of a glucose solution based on its relative optical absorbance at multiple probe wavelengths was proposed and verified. The relative absorbance at each of the probe wavelength was obtained with respect to the absorbance at a reference wavelength. The single reference wavelength (1310 nm) and a group of four different probe wavelengths (1064, 1550, 1685, 1798 nm) were selected to exhibit the glucose absorbance with opposite signs, thereby enhancing the accuracy of the prediction. The final glucose concentration was estimated by taking the average of the predicted values provided by the four probe wavelengths. The absorbance of the glucose solution for the path length of 5 mm was $-1.42{\times}10^{-6}\;AU$/(mg/dL) at the reference wavelength of 1310 nm and peaked at $+8.12{\times}10^{-6}\;AU$/(mg/dL) at 1685 nm. The concentration of the glucose solution was decently predicted by means of the proposed scheme with the standard error of prediction of ${\sim}28\;mg/dL$. In addition, the influence of the ambient temperature and the fat thickness upon the prediction of the glucose concentration was examined. The absorption change with the temperature was $-9.1{\times}10^{-5}\;AU/^{\circ}C$ in the temperature range of $26{\sim}40^{\circ}C$ at the reference wavelength, and $-2.08{\times}10^{-2}\;AU/^{\circ}C$ at 1550 nm. And the absorption change with respect to the fat thickness was +1.093 AU/mm at the probe wavelength of 1685 nm.

• Food Science of Animal Resources
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• v.29 no.4
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• pp.482-486
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• 2009

The aim of this study was to optimize a simple, fast, and economical analysis procedure for the determination of 16 different pesticides in raw milk via GC/MSD. Analyses were performed via gas chromatography with electron impact mass spectrometric detection in the selected ion monitoring mode (GC/MSD-SIM) using Pentachloronitrobenzene as the internal standard. The modified sample preparation methodology was based on the Pesticide Analytical Manual (PAM) of the FDA concerning fat extraction, ACN-ether partitioning, and clean-up of the Sep-Pak florisil cartridge. The modified methodology for the determination of the 16 pesticides was validated. The range of LOQs of the 16 pesticides was likely three times lower than their Maximum Residence Levels (MRLs). The recoveries of most of the pesticides were acceptable at the fortification levels of 0.5 and 1.0 ${\mu}g/mL$ and their RSD (%) level was less than 20%. None of the 16 pesticides were detected in the selected raw milk samples.