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http://dx.doi.org/10.5851/kosfa.2013.33.3.403

Evaluation on Microbiological Contamination Level of Raw Beef from Retail Markets in Seoul, Korea  

Ko, Eun-Kyung (Optipharm Inc)
Heo, Eun Jeong (Livestock Product Standard Division, Food Microbiology Division and Agro-Livestock & Fishery Products Policy Division, Ministry of Food and Drug Safety)
Kim, Young Jo (Livestock Product Standard Division, Food Microbiology Division and Agro-Livestock & Fishery Products Policy Division, Ministry of Food and Drug Safety)
Park, Hyun Jung (Livestock Product Standard Division, Food Microbiology Division and Agro-Livestock & Fishery Products Policy Division, Ministry of Food and Drug Safety)
Wi, Seong-Hwan (Veterinary Pharmaceutical Management Division, Animal and Quarantine Agency)
Moon, Jin San (Veterinary Pharmaceutical Management Division, Animal and Quarantine Agency)
Publication Information
Food Science of Animal Resources / v.33, no.3, 2013 , pp. 403-410 More about this Journal
Abstract
This study was performed to evaluate the microbiological contamination level of raw beef from retail markets in Seoul, Korea. The sampling and laboratory test were performed according to the procedure of "Standard for processing and ingredients specification of livestock product" and "Korean food code". Enterotoxin of Staphylococcus aureus isolates were detected using VIDAS$^{(R)}$ and PCR-based methods. Listeria monocytogenes serotyping and genotyping were carried out using Listeria antisera and L. monocytogenes Fingerprinting kit, respectively. A total of 48 samples were collected from 16 retail markets (butcher's shop: 5, department store: 6, supermarket: 5) in 2011. The level of total bacteria counts in the butcher's shop, department store and supermarket were $4.4{\times}10^3$ CFU/g, $3.9{\times}10^5$ CFU/g and $1.0{\times}10^4$ CFU/g, respectively. The concentrations of Escherichia coli of these three retail markets were $6.4{\times}10$ CFU/g, 7.6 CFU/g and $2.0{\times}10$ CFU/g, respectively. Salmonella species was not detected on all samples. However, S. aureus was isolated in the 3 samples (6.25%) from each type of three retail markets. L. monocytogenes was isolated in the 4 samples (8.3%) from department stores. The level of contamination of these foodborne bacteria was less than 100 CFU/g. The enterotoxin-encoding genes of S. aureus isolates were sea, seh, sei and sep gene. The gene similarity of L. monocytogenes isolated from two retail markets by Rep-PCR showed 57.8-98.1% and 68.1-98.1%, respectively. These results suggest that the HACCP guideline for environmental control in slaughterhouse and retail markets should be provided to prevent cross contamination and manage foodborne pathogens such as L. monocytogenes and S. aureus.
Keywords
retail markets; raw beef; HACCP; microbiological quality;
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Times Cited By KSCI : 8  (Citation Analysis)
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