• Korean Journal of Veterinary Service
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• v.30 no.1
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• pp.23-32
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• 2007

A total of 60 samples (38 domestic honeys and 22 foreign honeys) were collected from October in 2003 to May in 2004 in Incheon metropolitan area, and contents of important quality-related components in honeys were analyzed using methods of Korea Food Code. Most of the honeys were satisfied with Korean limit (21%) in moisture content even though 4 samples brought by Korean travellers and 1 sample in domestic honey (wild flower) were higher than the limit. The lowest mean level of moisture in the honeys was imported foreign honeys (16.95%). All of the samples were below of 0.6% in the analysis of ash contents. The highest content was imported foreign honey (0.19%) while the lowest content was in domestic acacia honey (0.05%). Acidity of domestic honeys (9.49-9.94meq/kg) was approximately half of that in foreign honeys. All samples were satisfied with Korean limit (40.0meq/kg). In the analysis of fructose and glucose, only three samples of foreign honeys were lower than Korean limit (sum of both ; 65%). The content of sucrose in all samples was less than 7%. Mean of Hydroxymethylfurfural (HMF) contents were 12.83-24.3mg/kg in domestic honey, and 31.34-45.58mg/kg in foreign honey. However, three samples brought by Korean travellers were not satisfied with Korean limit (${\leq}80mg/kg$). In conclusion, quality of domestic honey was better than that of foreign honey. Also, it may be needed that the honeys purchased by Korean travellers in foreign countries should be continuously monitored.

• Korean Journal of Food Science and Technology
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• v.50 no.6
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• pp.660-664
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• 2018

Honey is known to have a variety of health-promoting effects. Ethyl acetate fractions (EAFs) of four Korean domestic honeys from basswood, Korean raisin, chestnut, and acacia as well as a New Zealand manuka honey were analyzed comparatively to evaluate their antioxidative and antimelanogenic effects. The EAFs of basswood, Korean raisin, and chestnut honey had higher antioxidant capacities and tyrosinase inhibition activities than those of manuka honey. Pretreatment of B16F1 melanoma cells with EAFs at $100{\mu}g/mL$ resulted in relative retention of melanin contents as follows: acacia honey (141.0%)>basswood honey (134.3%)>manuka honey (131.5%)>Korean raisin honey (107.3%)>chestnut honey (88.0%). Intracellular tyrosinase activities of B16F1 melanoma cells were significantly (p<0.05) lowered by EAFs of Korean raisin and chestnut honey than by EAF of manuka honey. Consequently, these results suggest that Korean domestic honeys from different floral sources serve as good sources of antioxidative and antimelanogenic agents.

• The Korean Journal of Food And Nutrition
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• v.15 no.2
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• pp.158-164
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• 2002

This study examined for the investigation the effect of honey on antibacterial activity. The experimental honey were used the domestics, or chestnut honey, multiflower honey, acassia honey, native honey and the foreign, or manuka honey, clover honey, canola honey, and the artificial honey, made with the diluted solution of each 12.5%, 25.0%, 50.0%. The result of compared the occasion of added-catalase with not added-catalase about the honey's antibacterial activity on Staphylococcus aureus by agar well diffusion assay were as follows. When the catalase was not added, manuka honey antibacterial activity was superior to chestnut honey's in the diluted honey of 12.5% and on the occasion of the diluted honey of 25.0%, it was approved in the order of manuka honey > chestnut honey > multiflower honey 〉 native honey > clover honey > acassia honey and the occasion of the diluted honey of 50.0%, it was approved in the order of manuka honey > chestnut honey > canola honey > native honey > multiflower honey > clover honey > acassia honey(p > 0.01). The clear zone representing inhibition of growth in diluted honey of 12.5, 25.0, 50.0 % with non-treat catalase ranged from 5.85 to 6.60, 4.26 to 8.27, 5.24 to 11.49 mm, respectively. When the catalase was added, antibacterial activity only showed in the manuka honey of 12.5% and on the occasion of the diluted honey of 25.0%, manuka honey's antibacterial activity was superior to chestnut honey (p > 0.01). On the occasion of the diluted honey of 50.0%, antibacterial activity was high in the order of manuka honey > chestnut honey > clover honey > canola honey > native honey(p > 0.01). The correlation was approved significantly among the manuka honey, chestnut honey, clover honey, canola honey and native honey.

• Korean Journal of Agricultural Science
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• v.48 no.4
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• pp.899-910
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• 2021

This study was performed to suggest concentration methods leading to the production ofhoney with an excellent flavor by examining the effects of the concentration temperature and method on changes in 5-hydroxymethyl-2-furaldehyde (5-HMF) levels and the flavor components of honey. The 5-HMF contents of honey samples concentrated in a tray concentrator at 45, 50, 60, and 70℃ were 2.1, 2.3, 2.5, and 3.1 mg·kg^-1, respectively, demonstrating that the 5-HMF contents increased as the concentration temperatures were increased. The honey vacuum-concentrated at 70℃ showed a higher 5-HMF content than that at 60℃, similar to the tray-concentrated honey at different temperatures. The main and other minor flavor components of the honey were volatilized and significantly reduced after vacuum concentration. In the tray concentration, all of the honey samples concentrated at 40, 50, 60, and 70℃ showed flavor component patterns similar to each other, and most of the main and other minor flavor components in the honey were volatilized and significantly reduced after tray concentration. As such, most of the main and other minor flavor components of the honey were mostly removed at 70℃ after both the vacuum concentration and tray concentration processes. The effects of the concentration method and temperature on the viscosity, 5-HMF level, and flavor components of the honey were found to be significant in this study. Given that the components of honey were shown to undergo significant physicochemical changes depending on the concentration method used and temperature during laboratory-scale production, the concentration methods devised in this study can be applied industrially.

• Korean Journal of Veterinary Service
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• v.28 no.3
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• pp.203-213
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• 2005

Oxytetracycline, tetracycline, chlortetracycline and doxycycline in honey were separated by solid phase extraction (SPE) and determined with high performance liquid chromatography (HPLC) with UV/Visible detector. Analysis was carried out using following conditions: XTerra $C_8$ column $(3.9\times150mm\;i.d. 5{\mu}m)$, mobile phase composed of 0.01M oxalic acid : methanol : acetonitrile (820 : 80 : 100, v/v/v), isocratic pump at a flow rate of 0.9 ml/min. and $50{\mu}l$ of injection volume, UV/Visible detector with wavelength of 360nm. The calibration curves of four tetracyclines showed linearity $(\gamma^2>0.999)$ at concentration range of $100\~1,000 ng/ml$. The recoveries in fortified honey represented more than $70\%$ with low coefficient of variation $(<10\%)$ for concentration range of four tetracyclines. The detection limits for oxytetracycline, tetracycline, chlortetracycline and doxycycline were 13.8, 14.6, 26.2 and 24.9ng/g in acacia honey. respectively. We also monitored tetracyclines residue in domestic honey [n : 38, acacia (20), wild flower (18) ] and foreign honey [n=22, legally distributed (13), illegally distributed (9)] using modified Charm II screening and HPLC confirmation methods. Seven of the 60 samples $(11.7\%)$ were suspect positive using modified Charm II screening test. Chlortetracycline residue was found in one foreign honey (illegally distributed) tested at concentrations of 0.22 ppm. Conclusively, for more effective control of tetracyclines used in beekeeping should be further survey for residues in honey and also national guidelines (maximum residue limit : MRL) and methods should be obligatory.

• Korean Journal of Food Science and Technology
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• v.17 no.3
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• pp.155-162
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• 1985

The sugers and free amino acids of honey(Western and Domestic honey) and the changes of diastase activity during storage at various temperatures and HMF were studied. The average content of sugers in the western honey was 40.20% of fructose, 32.43% of glucose and 1.0% of sucrose. The average ratio of fructose to glucose was 1.24. In the case of the domestic honey, average content of sugers was 37.37% of fructose, 31.92% of glucose and 0.74% of sucrose and the average ratio of fructose to glucose was 1.19. Of the 16 amino acids detected, proline was the most predominant free amino acid in all of the honey studied, representing 39.59 to 46.86% of total free amino acids. Diastase activity of honey during the storage at $50^{\circ}C$ was rapidly decreased, but it was notably changed during storage at low temperature ($4^{\circ}C$) and room temperature ($20^{\circ}C$). The accumlated content of HMF were 0.12 to 0.47mg% during storage at 80 days at $20^{\circ}C$ and 5.35 to 8.71mg% after 3 weeks storage at $50^{\circ}C$.

• Korean Journal of Veterinary Service
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• v.34 no.1
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• pp.81-86
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• 2011

This study was designed to evaluate the beekeeper's consciousness for diagnosis and treatment on a disease of honey-bee in Daejeon and Jeollanamdo in Korea. The questionnaire consisted of 14 questions. Of 231 randomly selected respondents between February and May 2010, 47.6% was consult a 'club (colleague)', 89.2% treated a 'doctoring oneself', 84.4% was recognize a bee as 'domestic animal', 77.5% was not recognize a veterinarian as field of treatment, 38.5% was acquire know-how by a beekeeper. Therefore these results suggest that the beekeepers are relying a lot on individuals experience for the diagnosis and treatment on the disease of honey-bee, the apiculture techniques was gained much by oneself. Korean beekeeping association was played a role as well, but related-university, agriculture research & extension services and veterinary service laboratory was not played a role in the country.

• Korean Journal of Food Science and Technology
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• v.40 no.1
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• pp.8-14
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• 2008

This study was performed to establish analysis methods, and evaluated for grayanotoxin in domestic/foreign honey and wild honey. The molecular weight of grayanotoxins I, II and III, excluding grayanotoxin III that has been commercialized, were analyzed by LC-MS/MS. Then, the molecular structure of grayanotoxins I and II were analyzed by NMR. A total 111 samples (25 Korean honey, 21 Korean wild honey, 13 Korean honeycomb honey, 44 foreign honey, 8 foreign wild honey) were examined to determined whether or not each sample contained grayanotoxins I, II, and III. The honey samples were mixed with methanol and loaded into a tC18 cartridge, the filtrate was diluted with water, and the mixture was then analyzed by ESI triple-quadrupole LC-MS/MS. Grayanotoxins were only found in the foreign wild honey and were not detected in Korean honey, Korean honeycomb honey, or Korean wild honey. Three of the samples contained grayanotoxin I, II, and III, and one sample contained only grayanotoxins I and III. The lowest level for grayanotoxin I was 3.13 ${\pm}$ 0.00 mg/kg, and the highest level was 12.93 ${\pm}$ 0.01 mg/kg. The levels of grayanotoxin II were 0.84 ${\pm}$ 0.01 mg/kg, 0.92 ${\pm}$ 0.00 mg/kg and 1.08 ${\pm}$ 0.01 mg/kg, respectively. The lowest level of grayanotoxin III was 0.25 ${\pm}$ 0.01 mg/kg and the highest level was 3.29 ${\pm}$ 0.74 mg/kg. Through this study, safety management for foreign wild honey has been enabled.

• Korean Journal of Food Science and Technology
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• v.44 no.4
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• pp.401-410
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• 2012

This study examines the authenticity discrimination of the circulated honey by using stable isotope ratio methods. In the case of domestic honey, the range of ${\delta}^{13}C$ for the samples labeled as pure honey was about -27- -21‰ at the $C_3$ origin, and the range of that for artificial honey was over -19‰ at the $C_4$ origin. The range of ${\delta}^{13}C$ for all imported honey was over -27- -23‰ originating from the $C_3$ plant. According to the nectar-source, ${\delta}^2H$ and ${\delta}^{18}O$ for domestic honey were significantly different for 6 and 5 groups, respectively. However, we could not explain the detailed relationship as well as the geographical feature of ${\delta}^2H$ and ${\delta}^{18}O$. The difference for ${\delta}^2H$ and ${\delta}^{18}O$ in the wide range of latitude, such as between Australia and Canada, was more or less shown. However, it was difficult to find out the trends of ${\delta}^2H$ and ${\delta}^{18}O$ for imported honey versus the geographical information in the similar latitudinal country.

• Korean Journal of Veterinary Service
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• v.29 no.4
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• pp.451-457
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• 2006

This study was conducted to investigate the contents of lead (Pb) and cadmium(Cd) in honeys (n=60) from April to May in 2004 and to provide the scientific basis for heavy metal regulations of Korea Food Code. The samples were digested with acids, then analyzed for the contents of Pb and Cd by an inductively coupled plasma spectrometer (ICP). The contents of Pb and Cd [minimum-maximum (mean)] were 0.189-1.82 mg/kg (0.568) and not detected (ND)-0.016 mg/kg (0.0008) in domestic acacia honeys (n=20), ND-1.702 mg/kg (0.329) and ND-0.243 mg/kg (0.013) in domestic wild flower honeys (n= 18), ND-0.322 mg/kg (0.073), ND-0.027 mg/kg (0.002) in imported honeys (n=13), ND-3.754 mg/kg (0.671) and ND-0.658 mg/kg (0.073) in foreign honeys (n=9) brought by Korean travellers, respectively. According to the results, foreign honeys brought by Korean travellers were detected with the highest level of Pb and Cd. Therefore, we recommend that heavy metals of domestic and foreign honeys should be continuously monitored. It is also thought that these results could be the important references to establish the standard of Pb and Cd in honey.