Fig. 1. Antioxidant capacities of the ethyl acetate fractions from five different varieties of honey using oxygen radical absorbance capacity assay.
Fig. 2. Tyrosinase inhibition of the ethyl acetate fractions from five different varieties of honey.
Fig. 3. Cytotoxic effects of the ethyl acetate fractions from five different varieties of honey on B16F1 melanoma cells.
Fig. 4. Antimelanogenic effects of the ethyl acetate fractions from five different varieties of honey on B16F1 melanoma cells.
Fig. 5. Effects of the ethyl acetate fractions from five different varieties of honey on intracellular tyrosinase activity in B16F1 melanoma cells.
References
- Andrade P, Ferreres F, Amaral MT. Analysis of honey phenolic acids by HPLC, its application to honey botanical characterization. J. Liq. Chromatogr. Relat. Technol. 20: 2281-2288 (1997) https://doi.org/10.1080/10826079708006563
- Anklam E. A review of the analytical methods to determine the geographical and botanical origin of honey. Food Chem. 63: 549-562 (1998) https://doi.org/10.1016/S0308-8146(98)00057-0
- Baek Y, Kim YJ, Baik M-Y, Kim D-O, Lee H. Total phenolic contents and antioxidant activities of Korean domestic honey from different floral sources. Food Sci. Biotechnol. 24: 1453-1457 (2015) https://doi.org/10.1007/s10068-015-0187-8
- Bertolotto C, Busca R, Abbe P, Bille K, Aberdam E, Ortonne J-P, Ballotti R. Different cis-acting elements are involved in the regulation of TRP1 and TRP2 promoter activities by cyclic AMP: pivotal role of M boxes (GTCATGTGCT) and of microphthalmia. Mol. Cell. Biol. 18: 694-702 (1998) https://doi.org/10.1128/MCB.18.2.694
- Bogdanov S, Jurendic T, Sieber R, Gallmann P. Honey for nutrition and health: a review. J. Am. Coll. Nutr. 27: 677-689 (2008) https://doi.org/10.1080/07315724.2008.10719745
- Breathnach AS, Wyllie LM. Electron microscopy of melanocytes and melanosomes in freckled human epidermis. J. Invest. Dermatol. 42: 389-394 (1964) https://doi.org/10.1038/jid.1964.84
- Brenner M, Hearing VJ. The protective role of melanin against UV damage in human skin. Photochem. Photobiol. 84: 539-549 (2008) https://doi.org/10.1111/j.1751-1097.2007.00226.x
- Chang T-S. An updated review of tyrosinase inhibitors. Int. J. Mol. Sci. 10: 2440-2475 (2009) https://doi.org/10.3390/ijms10062440
-
Chung S-Y, Seo Y-K, Park J-M, Seo M-J, Park J-K, Kim J-W, Park C-S. Fermented rice bran downregulates MITF expression and leads to inhibition of
${\alpha}$ -MSH-induced melanogenesis in B16F1 melanoma. Biosci. Biotechnol. Biochem. 73: 1704-1710 (2009) https://doi.org/10.1271/bbb.80766 - El-Kased RF, Amer RI, Attia D, Elmazar MM. Honey-based hydrogel: in vitro and comparative in vivo evaluation for burn wound healing. Sci. Rep. 7: 9692 (2017) https://doi.org/10.1038/s41598-017-08771-8
- Eteraf-Oskouei T, Najafi M. Traditional and modern uses of natural honey in human diseases: a review. Iran. J. Basic Med. Sci. 16: 731-742 (2013)
- Han S, Lee K, Yeo J, Woo S, Kweon H, Nam S, Ho Y, Kim W. Whitening effect of the honey from Korea. Korean J. Apic. 25: 39-43 (2010)
-
Heo H-J, Cho H-Y, Hong B, Kim H-K, Kim E-K, Kim B-G, Shin DH. Protective effect of 4,5-dihydroxy-3,6,7-trimethoxyflavone from Artemisia asiatica against A
${\beta}$ -induced oxidative stress in PC12 cells. Amyloid-J. Protein Fold. Disord. 8: 194-201 (2001) https://doi.org/10.3109/13506120109007362 - Huang D, Ou B, Hampsch-Woodill M, Flanagan JA, Prior RL. Highthroughput assay of oxygen radical absorbance capacity (ORAC) using a multichannel liquid handling system coupled with a microplate fluorescence reader in 96-well format. J. Agric. Food Chem. 50: 4437-4444 (2002) https://doi.org/10.1021/jf0201529
- Jantakee K, Tragoolpua Y. Activities of different types of Thai honey on pathogenic bacteria causing skin diseases, tyrosinase enzyme and generating free radicals. Biol. Res. 48: 4 (2015) https://doi.org/10.1186/0717-6287-48-4
- Jung C. A note on the early publication of beekeeping of Western honeybee, Apis mellifera in Korea: Yangbong Yoji (Abriss Bienenzucht) by P. Canisius Kugelgen. J. Apic. 29: 73-77 (2014)
- Kassim M, Achoui M, Mustafa MR, Mohd MA, Yusoff KM. Ellagic acid, phenolic acids, and flavonoids in Malaysian honey extracts demonstrate in vitro anti-inflammatory activity. Nutr. Res. 30: 650-659 (2010) https://doi.org/10.1016/j.nutres.2010.08.008
- Kim HR, Kim H, Jung BJ, You GE, Jang S, Chung DK. Lipoteichoic acid isolated from Lactobacillus plantarum inhibits melanogenesis in B16F10 mouse melanoma cells. Mol. Cells 38: 163-170 (2015)
- Kim D-S, Park S-H, Kwon S-B, Li K, Youn S-W, Park K-C. (-)-Epigallocatechin-3-gallate and hinokitiol reduce melanin synthesis via decreased MITF production. Arch. Pharm. Res. 27: 334-339 (2004) https://doi.org/10.1007/BF02980069
- Kim Y-J, Uyama H. Tyrosinase inhibitors from natural and synthetic sources: structure, inhibition mechanism and perspective for the future. Cell. Mol. Life Sci. 62: 1707-1723 (2005) https://doi.org/10.1007/s00018-005-5054-y
- Kohsaka R, Park MS, Uchiyama Y. Beekeeping and honey production in Japan and South Korea: past and present. J. Ethnic Foods 4: 72-79 (2017) https://doi.org/10.1016/j.jef.2017.05.002
- Lee SK, Lee H. Antimicrobial activity of solvent fractions and bacterial isolates of Korean domestic honey from different floral sources. Food Sci. Biotechnol. 25: 1507-1512 (2016) https://doi.org/10.1007/s10068-016-0234-0
- Levy C, Khaled M, Fisher DE. MITF: master regulator of melanocyte development and melanoma oncogene. Trends Mol. Med. 12: 406-414 (2006) https://doi.org/10.1016/j.molmed.2006.07.008
- Masamoto Y, Ando H, Murata Y, Shimoishi Y, Tada M, Takahata K. Mushroom tyrosinase inhibitory activity of esculetin isolated from seeds of Euphorbia lathyris L. Biosci. Biotechnol. Biochem. 67: 631-634 (2003) https://doi.org/10.1271/bbb.67.631
- Nithitanakool S, Pithayanukul P, Bavovada R, Saparpakorn P. Molecular docking studies and anti-tyrosinase activity of Thai mango seed kernel extract. Molecules 14: 257-265 (2009) https://doi.org/10.3390/molecules14010257
- Pfeifer GP, Besaratinia A. UV wavelength-dependent DNA damage and human non-melanoma and melanoma skin cancer. Photochem. Photobiol. Sci. 11: 90-97 (2012) https://doi.org/10.1039/C1PP05144J
- Rigopoulos D, Gregoriou S, Katsambas A. Hyperpigmentation and melasma. J. Cosmet. Dermatol. 6: 195-202 (2007) https://doi.org/10.1111/j.1473-2165.2007.00321.x
- Sanchez-Ferrer A, Rodriguez-Lopez JN, Garcia-Canovas F, Garcia-Carmona F. Tyrosinase: a comprehensive review of its mechanism. Biochim. Biophys. Acta 1247: 1-11 (1995) https://doi.org/10.1016/0167-4838(94)00204-T
- Sapkota K, Park S-E, Kim J-E, Kim S, Choi H-S, Chun H-S, Kim S-J. Antioxidant and antimelanogenic properties of chestnut flower extract. Biosci. Biotechnol. Biochem. 74: 1527-1533 (2010) https://doi.org/10.1271/bbb.100058
- Ullah S, Khan SU, Saleh TA, Fahad S. Mad honey: uses, intoxicating/poisoning effects, diagnosis, and treatment. RSC Adv. 8: 18635-18646 (2018) https://doi.org/10.1039/C8RA01924J
- Unver N, Freyschmidt-Paul P, Horster S, Wenck H, Stab F, Blatt T, Elsasser HP. Alterations in the epidermal-dermal melanin axis and factor XIIIa melanophages in senile lentigo and ageing skin. Br. J. Dermatol. 155: 119-128 (2006) https://doi.org/10.1111/j.1365-2133.2006.07210.x
- Visavadia BG, Honeysett J, Danford MH. Manuka honey dressing: an effective treatment for chronic wound infections. Br. J. Oral Maxillofac. Surg. 46: 55-56 (2008) https://doi.org/10.1016/j.bjoms.2006.09.013
- Yoshioka S, Terashita T, Yoshizumi H, Shirasaka N. Inhibitory effects of whisky polyphenols on melanogenesis in mouse B16 melanoma cells. Biosci. Biotechnol. Biochem. 75: 2278-2282 (2011) https://doi.org/10.1271/bbb.100514
- Zumla A, Lulat A. Honey-a remedy rediscovered. J. R. Soc. Med. 82: 384-385 (1989) https://doi.org/10.1177/014107688908200704