• Journal of Applied Biological Chemistry
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• 제55권3호
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• pp.149-156
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• 2012

The effects of solvent type and concentration, solid/liquid ratio, extraction time and repeated extraction on recovery of keratinase from solid-state fermentation (SSF) of chicken feather by a local Streptomyces sp. NRC 13S were investigated in order to establish the experimental conditions for keratinase yield. Among solvents tested, 0.5% (v/v) glycerol was the best. Box-Behnken design was used to investigate the effect of relevant variables on keratinase recovery. The factors investigated were solid/liquid ratio (1:1.66-1:6.66 g/mL), glycerol concentration (0.5-5% v/v) and repeated extraction (1-5 cycle). The results showed that the maximum recovery of keratinase (6933.3 U/gfs) was obtained using 0.5 (v/v) glycerol as extracting solvent, in a solid/liquid ratio of 1:5 and three extraction cycles.

• Journal of Animal Science and Technology
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• 제63권6호
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• pp.1468-1472
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• 2021

Clostridium perfringens B20 was isolated from chicken feces collected from a local farm associated with Chung-Ang University (Anseong, Korea). The whole genome of C. perfringens B20 was sequenced using the PacBio RS II platform and assembled de novo. The genome is 2,982,563 bp long and assembled in two contigs. Annotation analyses revealed 2,668 protein-coding sequences, 30 rRNA genes, and 94 tRNA genes, with 28.2% G + C (guanine + cytosine) content. In silico genomic analysis revealed the presence of genes encoding a class IId bacteriocin, lactococcin A, and associated ABC transporter and immunity proteins, as well as a putative bacteriocin gene.

• 한국식품조리과학회지
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• 제25권5호
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• pp.600-605
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• 2009

본 연구는 시판되는 닭튀김류의 지방함량과 지방산 조성, 트랜스 지방산함량 및 산가를 조사하기 위해 총 20종을 대상으로 분석하였다. 그 결과는 다음과 같다. 20종의 닭튀김에서 추출된 지방함량은 $4.94{\sim}18.06%$의 다양한 범위를 나타내었다. 이러한 지방함량의 분포는 닭 자체의 지방함량이나 닭을 튀긴 기름의 종류, 튀김온도, 시간, 튀김옷의 성분차이 등 다양한 요인에 의한 차이라고 생각된다. 조사된 닭튀김의 주요 지방산 조성은 palmitic acid, stearic acid, oleic acid, linoleic acid 등이었다. 포화 지방산 중에서 palmitic acid($10.67{\sim}25.43%$)가 불포화지방산 중에서는 oleic acid($29.78{\sim}67.85%$)와 linoleic acid($8.42{\sim}39.54%$)가 가장 많았다. 식품 100 g당 함유되어 있는 트랜스 지방산 함량은 0.1 g 이하의 낮은 함량을 보여주었으며 닭튀김에 함유된 지방을 추출하여 산가를 측정한 결과 20종의 닭튀김의 결과치는 식품공전상 튀김식품의 산가 규격을 초과하지 않는 양호한 결과를 나타내었다.

• 대한수의학회지
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• 제29권4호
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• pp.503-515
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• 1989

Critical parameters affecting sensitivity and specificity of an enzyme-linked immunosorbent assay(ELISA) for detection of antibodies to avian infections bronchitis virus(IBV) were standardized. By adopting the optimized conditions an equation calculating ELISA antibody titers from the observations at single serum dilution was formulated. The purified antigen of IBV Mass-41 strain was dispensed into polystyrene microplate wells at a concentration of 300ng per well($100{\mu}l$) and the plates were coated by completey drying at $37^{\circ}C$. Diluted chicken serum and horseradish peroxidase conjugated goat anti-chicken IgG were added in order in $100{\mu}l$ volumes per well and allowed to react for 30 minutes each at room temperature. Just before use and after each reaction the plates were washed three times with distilled water. Finally o-phenylenediamine solution was added as an enzyme substrate. After incubation for another 15 minutes at room temperature absorbances were read at 492nm. Hyperimmune serum against Mass-41 strain was used as internal reference positive(IRP) serum. After repeated titration of IRP and negative serum, a constant titer of IRP was determined. Serum titrations were carried out for various sample sera together with IRP and negative sera and the observed titers of sample sera were corrected by reflecting the ratio between observed and constant titers of IRP serum. These corrected titers of the sample sera were plotted against sample/positive(S/P) OD ratios. All the OD's measured in the serum titrations were also corrected by substracting negative serum OD. The following equation was formulated from the above data; $Log_{10}$ ELISA titer=$5.568({\log}_{10}S/P)+4.161$ Thus it was possible to calculate ELISA titer by measuring absorbance at 1/400 single serum dilution. Titer measured by cross ELISA tests employing Mass-41 strain and three local IBV isolates were similar. These results suggest that the ELISA tests standardized in this study can be used for evaluating not only vaccinal immunity but also for infection status against fields IBV's.

• Journal of Animal Science and Technology
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• 제66권5호
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• pp.1069-1078
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• 2024

Woody breast (WB) is one of muscle myopathy found in chicken breast, characterized with enlarged size and extremely stiff texture. The WB condition is one of the most prevalent quality issues in the modern poultry industry. WB has been shown to be heritable, but no effective detection method of WB severity in live birds exists for the selection purpose. The objective of this study was to determine potential of a non-invasive, portable digital palpation device as WB detection method that can be used for the selection to estimate the heritability of WB. The physical and functional properties of WB was also investigated in comparison with normal breast (NB). Two hundred ten breast muscles were obtained from a local processing plant one day after harvest and sorted based on WB scoring (1 for NB and 2 and 3 for WB). The samples were subjected to physical and physicochemical analyses, determining biomechanical properties (muscle tone, stiffness, elasticity, relaxation, and creep), pH, color, cooking yield, and texture (firmness and compression energy were used for raw meat and shear force and energy for cooked meat). The least squares means of the following variables were significantly different between WB and NB (p < 0.01): stiffness (603.4 vs 565.8; N/m), and elasticity (1.40 vs 1.55). However, relaxation and creep were not significantly different (p > 0.05). These results collectively showed that biomechanical properties of WB differ from NB. The degree of muscle stiffness in WB can be considered as a trait to be selected. The WB score showed strong negative correlations with cooking yield (-0.77) and cooked L^*(-0.74), which means that as the breast becomes harder, the cooking yield decreases, and the color becomes darker after cooking. The WB score showed high correlations with physical and functional characteristics and exhibited strong correlations with the biomechanical properties measured by the device. Therefore, the results indicated that the digital palpation device has potential to detect the WB severity (degree of stiffness) of breast muscle.

• Journal of Microbiology and Biotechnology
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• 제30권4호
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• pp.526-532
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• 2020

A bacterial strain, designated B301^T and isolated from raw chicken meat obtained from a local market in Korea, was characterized and identified using a polyphasic taxonomic approach. Cells were gram-negative, non-motile, obligate-aerobic coccobacilli that were catalase-positive and oxidase-negative. The optimum growth conditions were 30℃, pH 7.0, and 0% NaCl in tryptic soy broth. Colonies were round, convex, smooth, and cream-colored on tryptic soy agar. Strain B301^T has a genome size of 3,102,684 bp, with 2,840 protein-coding genes and 102 RNA genes. The 16S rRNA gene analysis revealed that strain B301^T belongs to the genus Acinetobacter and shares highest sequence similarity (97.12%) with A. celticus ANC 4603^T and A. sichuanensis WCHAc060041^T. The average nucleotide identity and digital DNA-DNA hybridization values for closely related species were below the cutoff values for species delineation (95-96% and 70%, respectively). The DNA G+C content of strain B301^T was 37.0%. The major respiratory quinone was Q-9, and the cellular fatty acids were primarily summed feature 3 (C_16:1 ω6c/C_16:1 ω7c), C_16:0, and C_18:1 ω9c. The major polar lipids were phosphatidylethanolamine, diphosphatidyl-glycerol, phosphatidylglycerol, and phosphatidyl-serine. The antimicrobial resistance profile of strain B301^T revealed the absence of antibiotic-resistance genes. Susceptibility to a wide range of antimicrobials, including imipenem, minocycline, ampicillin, and tetracycline, was also observed. The results of the phenotypic, chemotaxonomic, and phylogenetic analyses indicate that strain B301^T represents a novel species of the genus Acinetobacter, for which the name Acinetobacter pullorum sp. nov. is proposed. The type strain is B301^T (=KACC 21653^T = JCM 33942^T).

• 한국동물위생학회지
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• 제23권3호
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• pp.271-279
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• 2000

This study was carried out to provide the fundamental information for development of proper vaccination program against infectious bursal disease(IBD) to the local chicken farms. The antigen detection was peformed from 8 samples of bursa of Fabricius with agar gel precipitation(AGP) and indirect immunofluorescent assay(IFA), And also, the antibodies in serum samples were detected by the various serological methods such as commercial ELISA assay, AGP and virus neutralization(VN) test. 1. The antigen detection rates were 25% for AGP which is 2 out of 8 farms and 10 out of 40 bursas, and 25% which Is 2 out of 8 farms and 20% 8 out of 40 bursas for IFA, respectively. 2. The mean titer of maternal antibody (>3,000) existed until 10 days of the age with ELISA-GMT. 3. The antibody positive rates which are over 80% showed until 5 days of the age with ELISA and at 10 days of the age with AGP except one, but none of them showed from 1 day of the age. This report came to conclusions that both the protective maternal antibody titers and the antigen positive rates were significant until at the 10 days of the age.

• 미생물학회지
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• 제43권3호
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• pp.186-192
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• 2007

제주도를 포함한 전국규모로 15개 시 도에 위치한 도계장에서 직접 수거한 도계육에 대하여 미생물 오염도 조사로서 총세균수, 대장균군수 및 포도상구균수에 대해서 조사를 하였다. 특히 주요 인수공통 병원성 세균들인 Salmonella 속균과 Campylobacter jejuni, Listeria monocytogenes, Staphylococcus aureus, 그리고 E. coli O157:H7 균에 대해서도 함께 조사를 하였다. 그리고 도계장에서 보다 안전하고 위생적인 처리를 위해서 가장 흔히 사용되는 방법인 염소(20mg/L포함)를 첨가한 염소수로서 수세한 처리계육과 그렇지 않은 비처리 도계육 상호간에 대해서도 Salmonella 속균의 분리율과 기타 균종의 억제효과에 대해서도 비교조사를 수행하였다. 먼저 예비조사로서 강원지역을 비롯한 전국 6개 시 도의 도계장 유래 계육에 대해서 세균오염도의 비교조사에서는 포도상구균수, 대장균군수 및 총세균수의 순서로 오염도를 나타내었다. 이 성적은 시판계육의 미생물 오염도 수준보다는 $10{\sim}100$배 이상 낮은 오염도 성적이었다. 그리고 동일계육에 대한 Salmonella 속균의 분리율은 63.3%(19/30)였고, S. enteritidix (33.3%)가 가장 지배적인 혈청형이었으며, 동시에 S. typhimurium (3.3%), S. muenchen (30.0%)도 분리되었다. 하편, 염소수로 세척한 도계육은 총세균수와 대장균군수의 비교조사에서 비처리 계육보다도 약 100배정도로 균수의 억제효과를 보였다. 반면에 포도상구균수에서는 양자간에 뚜렷한 차이를 보이지 알았다. 또한 Salmonella 속균에 대한 억제효과의 비교조사에서도 20 ppm의 염소처리로서는 포도상구균과 마찬가지로 Salmonella 속균에 대해서도 뚜렷한 억제효과를 발휘하지 못한 것으로 확인되었다. 한편 보다 확대된 규모의 조사결과에서 제주도를 포함한 부산, 경남, 대구, 경북, 전남, 광주, 전북, 충남, 대전, 충북, 강원, 서울, 인천 및 경기지역을 포함하여 총 15개 시 도의 도계장 수거계육에서 Salmonella속균은 58.3%(67/115)에서 분리되었고, S. muenchen (57.3%)과 S. enteritidis (22.7%)가 대부분을 차지하였고, 인수공통병원균 중에서는 L. monocytogenes(43.5%), C. jejuni(37.4%), S. aureus(30.4%)의 순서로 분리되었으나, E. coli O157:H7은 국내 계육에서 전혀 분리되지 않았다. 결과적으로 도계육이 위생적이며 안전하게 시판되기 위해서는 최종적인 도계공정 이후 다양한 유통과정에서 발생될 수 있는교차 및 추가오염의 기회를 줄이기 위한 보다 철저한 위생관리 대책과 보완대책이 필요하다는 사실을 이 성적을 통하여 비로소 확인할 수 있었다.

• 비교문화연구
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• 제29권
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• pp.177-199
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• 2012

Nowadays migrations between the countries are getting each day more active and a mixture of people with different cultural backgrounds strengthens the hybridism, whereupon the point of view on the migration of Koreans has been changed from a diasporan perspective to an transnational one. The expansion of the transnationalism affects the identity of the subsequent generations of the immigration giving way to a psychologically deterritorialized social identity, which is an important consideration in the study of subsequent generations of the immigration. This study examined the Korean immigration history and the current status of the Korean community in Spain since the 1960s. The early immigrants like fishing vessel crew members, instructors of Taekwondo, chicken sexers who, despite the limits of foreigners in Spain, went to find out the source of life with no hesitation, overcame the difficulties of the early settlers and finally managed to achieve local success. Current history of immigration that began in the mid-1960s went through the maturity during the 1970s and 80s and the Koreans in Spain these days, who have undergone differentiation in the occupational structure because of the crisis of pelagic fisheries, face new challenges due to changes in the environment of the country they reside. The Korean community is regarded as an 'exemplary minority group (Model Minority)' even though it is a minority group in number. Now that the interest for overseas Koreans is higher than ever at home and abroad, due to the economic growth of the Republic of Korea, the spread of its cultural influence -the Korean Wave as proof-, and the overseas Koreans suffrage conducted, the research of the Korean residents in Spain will be important as an advanced research of an unexplored field, as well as an opportunity to broaden the horizons of the existing study area that has mainly dealt with major regions only.

• 한국식품과학회지
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• 제45권1호
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• pp.126-132
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• 2013

본 연구를 통해 중국소비자들에게 한식 양념된 후 진공저온에서 조리된 닭고기 버거와 중국 현지에서 시판 중인 닭고기 패티버거의 기호도 차이를 조사하기 위해 20-30대 북경 거주 여성을 대상으로 외식 성향, 외식 선택 속성, 한식과 한국에 대한 인식에 대한 조사와 버거 3종에 대한 소비자 기호도 조사를 실시한 결과는 다음과 같다. 1. 외식 빈도에 대한 질문에는 일주일에 1-2회라는 답변이 가장 많았으며 중식당과 패스트푸드 레스토랑이 1, 2위로 나타났다. 95.2%가 보통 이상으로 패스트 푸드를 좋아한다고 답하였으며, 응답자의 47.1%는 일주일에 1-2회 패스트 푸드를 먹는다고 답하였다. 평균 외식 비용은 20위엔 이상-50위엔 미만이 56.2%이라고 답하였으며, 월평균 외식 지출 비용은 500위안 미만 지출한다는 답변이 가장 많았다. 중국 여성이 외식을 하는데 있어서 가장 중요하게 생각하는 속성은 맛(M=6.5)에 대한 속성이었으며 건강(M=6.0), 기분 전환(M=5.6)의 순으로 중요하다고 답하였다. 2. 한국 음식에 대한 관심 정도에는 60.0% 이상이 관심이 있다고 답하였고 한국의 이미지에 대한 질문은 응답자의 65.3%가 긍정적이라고 답하였으며, 한국 음식의 이미지 역시 62.8%이 긍정적이라고 답하였다. 한국 음식을 좋아하냐는 질문에 90.1%가 좋다고 답하였고 그 이유는 맛이 좋기 때문에(63.6%), 모양과 색상이 좋기 때문(50.4%)이라고 답하였다. 3. 버거에 대한 소비자 기호도는 전반적 기호도, 외관 및 구매의도에서 BB가 SB, AB에 비해 유의적으로 높았으며 향미와 질감 부분에서는 세 시료간의 유의적인 차이가 나타나지 않았다. SB의 경우 평균적인 점수는 AB, BB에 비해 낮은 것으로 조사되었으나 전반적 기호도, 외관에 대한 기호도와 구매의도에서는 AB와 유의적인 차이가 나지 않은 것으로 조사되었다. 패티 외 식재의 변경, 향미, 질감의 보완을 통해 한식 스타일로 개발된 SB의 중국진출 가능성을 고려해 볼 수 있겠다. 4. SB가 좋은 이유는 향미는 32.7%, 맛은 26.1%, 질감은 17%, 기타 응답은 13.1% 및 전체적인 조화가 11.1%라고 답하였다. 싫어하는 이유는 향미는 33.7%, 맛은 25.6%, 질감은 23.3% 및 기타 응답은 17.4%로 답하였다. 그중 세부 항목은 조화롭지 않은 향미, 부재료의 향미, 소스의 강한 향미, 기름진 맛, 쓴맛, 단맛, 감칠맛이 싫다고 하였다. 친숙치 않은 식감과 패티의 질김과 씹힘성이 좋지 않으며 전체적으로 조화롭지 못하다는 응답이 있었다. 본 연구를 통해 중국시장에서의 진공저온조리를 통한 한식 외식 상품의 가능성을 살펴볼 수 있었으며, 연구 결과는 중국 현지에서 적용 가능한 다양한 상품 개발의 기초 자료로 활용될 수 있을 것이다.