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http://dx.doi.org/10.3839/jabc.2012.023

Optimization of Extraction Parameters for Keratinase Recovery from Fermented Feather under Solid State Fermentation by Streptomyces sp. NRC 13S  

Shata, Hoda Mohamed Abdel Halim (Microbial Chemistry Department, National Research Center)
Farid, Mohamed Abdel Fattah (Natural and Microbial Products Department, National Research Center)
Publication Information
Journal of Applied Biological Chemistry / v.55, no.3, 2012 , pp. 149-156 More about this Journal
Abstract
The effects of solvent type and concentration, solid/liquid ratio, extraction time and repeated extraction on recovery of keratinase from solid-state fermentation (SSF) of chicken feather by a local Streptomyces sp. NRC 13S were investigated in order to establish the experimental conditions for keratinase yield. Among solvents tested, 0.5% (v/v) glycerol was the best. Box-Behnken design was used to investigate the effect of relevant variables on keratinase recovery. The factors investigated were solid/liquid ratio (1:1.66-1:6.66 g/mL), glycerol concentration (0.5-5% v/v) and repeated extraction (1-5 cycle). The results showed that the maximum recovery of keratinase (6933.3 U/gfs) was obtained using 0.5 (v/v) glycerol as extracting solvent, in a solid/liquid ratio of 1:5 and three extraction cycles.
Keywords
Box-Behnken design; extraction; keratinase; optimization; solid-state fermentation;
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