• Journal of Microbiology and Biotechnology
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• v.25 no.8
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• pp.1339-1348
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• 2015

Until now, considerable effort has been made to engineer novel nitrogen-fixing organisms through the transfer of nif genes from various diazotrophs to non-nitrogen fixers; however, regulatory coupling of the heterologous nif genes with the regulatory system of the new host is still not well understood. In this work, a 49 kb nitrogen fixation island from P. stutzeri A1501 was transferred into E. coli using a novel and efficient transformation strategy, and a series of recombinant nitrogen-fixing E. coli strains were obtained. We found that the nitrogenase activity of the recombinant E. coli strain EN-01, similar to the parent strain P. stutzeri A1501, was dependent on external ammonia concentration, oxygen tension, and temperature. We further found that there existed a regulatory coupling between the E. coli general nitrogen regulatory system and the heterologous P. stutzeri nif island in the recombinant E. coli strain. We also provided evidence that the E. coli general nitrogen regulator GlnG protein was involved in the activation of the nif-specific regulator NifA via a direct interaction with the NifA promoter. To the best of our knowledge, this work plays a groundbreaking role in increasing understanding of the regulatory coupling of the heterologous nitrogen fixation system with the regulatory system of the recipient host. Furthermore, it will shed light on the structure and functional integrity of the nif island and will be useful for the construction of novel and more robust nitrogen-fixing organisms through biosynthetic engineering.

• Proceedings of the Korea Inteligent Information System Society Conference
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• 2000.11a
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• pp.397-406
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• 2000

대부분의 국방정보시스템의 소프트웨어는 높은 가용성, 신뢰성, 신속성, 정확성 등을 요구하는 대규모이면서 복잡한 실시간 시스템이다. 이러한 국방정보시스템의 소프트웨어 개발사업에 있어서 저비용 고효율의 미개국방경영 건설을 위하고 강한 전투력을 육성하기 위해서는 국방정보시스템의 효율적인 소프트웨어 개발사법이 요구된다. 따라서, 국방정보시스템의 소프트웨어 사업관리자가 개발사업을 관리하고 감독하는데 있어서 개발자와 사용자간의 조정 및 통제 기능을 수행하고 해당 국방정보시스템의 특성을 파악하여 성공적인 사업수행을 할 수 있도록 기술적인 사업관리 측면에서 구체적이고 상세화된 방안/지침을 제공하기 위한 전문가시스템의 지식베이스 도메인 지식개발에 관한 연구이다. 기존의 국방정보시스템의 사업관리자가 경험을 동해 축적해 온 기술, 정책, 아이디어, 노하우 등에 대한 지식을 습득하고 사업 관련자료에서 제시한 소프트웨어 생명주기 단계별 방안이나 지침 등을 바탕으로 하여 식별된 사실이나 내용을 지식베이스로 구축하여 국방정보시스템의 사업관리자가 필요로 할 때 설명모듈을 거쳐 임무 및 세부활동사항을 게시하여 줌으로써 사업관리 경험이 부족하거나 사업관리자가 교체되었을 때 사업관리자들이 업무를 지속적으로 연계시켜 임무수행이 가능하도록 기초/기반 여건을 제공하고자 한다. 본 논문은 국방정보시스템의 소프트웨어 개발사업에서 소프트웨어 생명주기 단계별 사업관리자의 임무 및 세부활동사항 지원용 전문가시스템을 개발할 때 이용할 수 있도록 도메인 지식을 개발하는 것이며 논문의 결과를 활용시 기대되는 효과는 본문을 참고 바란다.의 장점을 취합하여 설계되었다. 본 시스템은 기존의 UN/EDIFACT표준을 사용하고 있는 EDI환경과 기존 VAN 방식의 EDI 중계 시스템과 연동되며, 향후 관세청의 XML/EDI 표준 시행을 미리 대비하는 선도연구로서 자리매김이 된다. 본 연구에서는 개발된 XML/EDI 통관시스템은 향후, 서비스의 최대 걸림돌이 되어왔던 값비싼 EDI 사용료의 부담에서 벗어날 수 있게 할 것이며, 저렴한 EDI구축/운영 비용으로 전자문서교환의 활성화와 XML이 인터넷 기반의 문서유통 표준으로 자리매김할 수 있는 중요한 계기가 될 것이다.재무/비재무적 지표를 고려한 인공신경망기법의 예측적중률이 높은 것으로 나타났다. 즉, 로지스틱회귀 분석의 재무적 지표모형은 훈련, 시험용이 84.45%, 85.10%인 반면, 재무/비재무적 지표모형은 84.45%, 85.08%로서 거의 동일한 예측적중률을 가졌으나 인공신경망기법 분석에서는 재무적 지표모형이 92.23%, 85.10%인 반면, 재무/비재무적 지표모형에서는 91.12%, 88.06%로서 향상된 예측적중률을 나타내었다.ting LMS according to increasing the step-size parameter $\mu$ in the experimentally computed. learning curve. Also we find that convergence speed of proposed algorithm is increased by (B+1) time proportional to B which B is

• Journal of the Korea Academia-Industrial cooperation Society
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• v.17 no.10
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• pp.345-356
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• 2016

The purpose of this study was to develop a scale to evaluate the safety competency of perioperative nurses and to examine its validity and reliability. The research process included the development of preliminary items through literature reviews and the construct validity examination of the conceptual framework for the preliminary scale. The content validity of the identified items was evaluated by experts, resulting in 30 preliminary items. The participants involved in testing the validity and reliability of the preliminary scale were 377 perioperative nurses. The data were analyzed using exploratory factor analysis, parallel analysis, confirmatory factor analysis, and internal consistency. To verify the construction factor of the preliminary scale, exploratory factor analysis and parallel analysis were performed, resulting in 3 factors and 27 items. The internal structure of the scale was schematized using confirmatory factor analysis and the goodness of fit of the final research model was very appropriate, as shown by the values of TLI=.90, CFI=.91, RMSEA=.07, and SRMR=.07. The final scale consisted of 27 items and 3 factors including knowledge (6 items), skill (13 items), and attitude (8 items). Cronbach' ${\alpha}$ for the final scale was .94, showing good internal consistency. This safety competency scale can be used for assessing the competency of perioperative nurses regarding patient safety, for developing patient safety studies and for the career development of perioperative nurses.

• Korean Journal of Comparative Education
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• v.18 no.4
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• pp.141-162
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• 2008

Many e-learning programs in Korea use lecture as a main instructional method. A meta-analysis study reports that e-learning programs using lectures were the most ineffective. In addition, many researchers in the field of distance education contend that the active participation of learners is the key to the success of e-learning. These imply why we can easily find many people who don't have good impression about e-learning. The quality of e-learning depends on the application of appropriate pedagogy. This study aims to present the implications for the improvement of e-learning programs in the Republic of Korea by analyzing the psychological characteristics reflected on the e-learning programs in the U. S. that have been improved through design research for a long time. The result shows that the e-learning programs in the U. S. have five major psychological aspects, i.e., reflective thinking, collaborative interaction, knowledge construction, situated action, and utilizing multiple representations. Consequently, this study suggests that e-learning programs in the Republic of Korea need to reflect learning principles such as learning by doing, situated learning, collaborative learning, learning with multiple representations in order to improve the quality.

• Cross-Cultural Studies
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• v.48
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• pp.325-359
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• 2017

The works of a literary and cultural historian Franco Moretti are conspicuous in many ways. Trained in Marxism and Russian formalism, he participated in the construction of the New Left in England. Also, he interestingly interpreted the socialization of the individual through the genre of bildungsroman. Then, he shifted his research interest to the notion of world literature, and to explore its global scale, he developed his own quantitative approach combined with advanced computer technology in digital humanities. His recent publication reveals that Moretti conducted a social critique of the European bourgeois culture with his new quantitative method. His macroscopic view of literature and use of cutting-edge technology in his research inspire historians of Korean literature located in the so-called periphery of world literature. Therefore, the purpose of this study is to examine the idea of world literature outlined by Franco Moretti by reviewing his method called "distant reading" and examples of such an approach. His distant reading is to construct a macroscopic archive through inclusion of forgotten works from literary history and to analyze morphological patterns that frequently appear in the archive. His book entitled Graphs, Maps, Trees is a collection of examples of which he applied distant reading. By delving into such cases, I will raise questions about Moretti's macroscopic perspective of world literature in conjunction with Korean literature. As located at the periphery of global circulation of literary knowledge, Korea appropriated Western genres, established its literary institutions, and developed book markets through modern newspapers and magazines. This experience of furthering modern literature through periodicals would provide another view to revisit Moretti's world literature.

• The Korean Journal of Archival Studies
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• no.19
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• pp.57-102
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• 2009

Recently, distribution of record via web and coefficient of utilization are increase. so, Archival information service using website becomes essential part of record center. The main point of archival information service by website is making record information retrieval easy. It has need of matching user's request and representation of record resources correctly to making archival information retrieval easy. Archivist and record manager have used various information representation tools from taxonomy to recent thesaurus, still, the accuracy of information retrieval has not solved. This study constructed record retrieval system based on Topic Map by modeling record resources which focusing on description metadata of the records to improve this problem. The target user of the system is general web users and its range is limited to the president related sources in the National Archives Portal Service. The procedure is as follows; 1) Design an ontology model for archival information service based on topic map which focusing on description metadata of the records. 2) Buildpractical record retrieval system with topic map that received information source list, which extracted from the National Archives Portal Service, by editor. 3) Check and assess features of record retrieval system based on topic map through user interface. Through the practice, relevance navigation to other record sources by semantic inference of description metadata is confirmed. And also, records could be built up as knowledge with result of scattered archival sources.

• Communications of Mathematical Education
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• v.33 no.3
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• pp.231-254
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• 2019

As the teacher-centered education is transformed into learning-centered education, the active knowledge construction of learner becomes important. Along with this change, project-based learning is receiving attention. However, there are many problems to apply the results of research to pre-service teachers because most of the previous studies have focused on elementary middle high school students. Therefore, this study examined the changes in perceptions of pre-service mathematics teachers while project-based learning progressed for one year. Pre-service mathematics teachers had less experience with project-based learning than group discussions, group activities. Also their perceptions was classified as five factors: 'Instructional effects', 'Application and participant of project-based learning', 'Achievement', 'Diffusion of project-based learning', 'Student motivation'. Pre-service mathematics teachers responded positive changes in perception that project-based learning could be motivated to students but they responded negative changes in perception that project-based learning could be distributed to school. They responded positive changes in perception that teachers can show their achievements by project-based learning but they responded negative change in perception that teachers would be fun to apply project-based learning. Also, they responded positive changes in perception that teachers and students were easy to apply or utilize project-based learning.

• 재활복지
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• v.21 no.4
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• pp.119-147
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• 2017

The purpose of this study is to analyze the awareness and needs for community based Telerehabilitation(TR) service by carrying out a online survey targeting individual with disabilities. By using the survey, we attempt to utilize the results as the basis for the health promotion and effective rehabilitation service to the disabilities in the community. Survey was conducted on the 162 target users, who use center for independent living, are registered as disabled. There are 34 questions in total. The results of the study are as follows. First, 74.7% of respondents said 'they had never heard of TR service'. 68.3% of the respondents said that 'they want to use the service' and 78% are positive about the service. Second, 84% of them said 'they have an intend to participate the service', 87.1% of the respondents answered that they needed 'very necessary' and 'necessary' in the question of necessity. Third, as a results of searching the requirements for TR service, it was found that there are requirements such as service system and construction considering the characteristics of the disabled, service provision of rehabilitation experts, and various program composition. Based on the results of this study, we suggest the development of a differentiated TR system program to improve the quality of rehabilitation for individuals with disabilities by considering the reality of rehabilitation treatment of community disabled people and considering the knowledge and needs of TR system.

• Journal of The Korean Association For Science Education
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• v.39 no.2
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• pp.295-305
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• 2019

In previous study, visual representation competence taxonomy (VRC-T), which is composed of two dimensions, was developed for the purpose of promoting effective visual representation use and research in science education. In this study, elementary school students' visual representation competence for shadow phenomenon was investigated using VRC-T. In terms of visual representation competence, 'interpretation' was the highest score, followed by 'construction' and 'integration'. It also showed that students' visual representation competence was not high even after learning shadow-related units in the regular curriculum. On the other hand, text-based scientific knowledge was not correlated with all categories of visual representation competence. This indicates that there is a need to emphasize visual representation more in science class. Finally, hierarchical relationship among cognitive processes of VRC-T was explored according to ordering theory. If the tolerance level is somewhat loosened, a linear hierarchical relationship was found between the six cognitive processes. This suggests that VRC-T is an analytical framework that can be useful when designing assessment tools, tasks, and science class activities to enhance visual representation competence.

• The Journal of Korean Philosophical History
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• no.23
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• pp.129-154
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• 2008

This essay is a landscape of core values and relevant evidences about Creative Leadership in order to make sketches of Korean-style leadership framework. Glancing through this work, you can smell the distinctions of Korea creative leaders, particularly, in the aspects of procommunity pragmatism and ordinary innovation. Most Korea creative leaders were social mentors and masters who had enlarged their influences from the individual domain to social area in all the dimensions of human life. At the individual stage, the Creative Leadership suggests 'Jungki(正己)' as a core value of themselves, which is materialized as such competencies as self-assertiveness, observation and experimentation, and strong inquiry. Because most of them were technical masters dealing with new methodology and new knowledge including alchemy, medicine and weapons, the creative leaders show us very unique core values and competencies in the life sequences of the individuation, relationship, organization and community unlike other Korean-style leaderships and global practices. You can taste and compare these things: 'Kihyulsangtong(氣血相通)' as a core value and respect- for- others, style analysis, persuasion as core competencies in the stage of relationship, nextly, 'Suhshi(授時) & Jungeum(正音)' as a core value and coordination and adjustment, teamwork as core competencies in the stage of organization, finally, 'Hunmin(訓民) & Hwalin(活人)' as a core value and vision-building, social benefiting, ordinary innovation as core competencies in the stage of community. The value of this study is focused on the construction of Korean-style leadership framework based on the Creative Leadership through vivid stories about great leaders in our history.