• 제목/요약/키워드: Kit development

검색결과 482건 처리시간 0.029초

Development of Rapid Diagnostic Technology for Pig Disease (2) - Rapid detection of PPE in the pig feces -

  • Kim, Hyuck-Joo;Hong, Jong-Tae;Yu, Byeong-Kee;Kim, Giyoung;Kim, Suk
    • Journal of Biosystems Engineering
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    • 제38권2호
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    • pp.121-128
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    • 2013
  • Purpose: Porcine proliferative enteropathy (PPE), caused by the obligate intracellular bacterium Lawsonia intracellularis, is a widely distributed disease throughout the world causing substantial economic loss. In order to diagnose PPE rapidly, the rapid kit was developed and tested. Methods: In this study, a rapid kit was developed to screen the PPE rapidly at the pig farm. Also, occult blood test with fecal occult blood (FOB) kit was done for detecting the blood in pig feces which might be the evident of hemorrhagic PPE. For developing the kit, we tested fecal samples of PPE infected pigs diagnosed by polymerase chain reaction (PCR) method. Results: With the developed rapid kit, Lawsonia intracellularis was detected in high density emulsion of ileum. On the other hand, the test result of detecting Lawsonia in feces showed too high non-specific response. In addition, nevertheless the FOB test result showed that blood evident could be founded in pig feces, the diagnosing result was not fit to PCR test result, which shows blood in pig feces could be from not only hemorrhagic PPE but also many reasons. Conclusions: To deal with the PPE effectively, it will be better for farmers to screen the PPE in earlier stage with easy and rapid diagnosing tool on farm. This study found out that the rapid kit could detect the Lawsonia intracellularis and hemoglobin in pig feces. However, the non-specific response to negative samples of PPE was too high to use at a pig farm. Further research is needed for lowering the non-specific response with the rapid kit.

Development and Application of Horticultural Kit for Children's Horticulture Education

  • Kwack, Hye Ran
    • 인간식물환경학회지
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    • 제24권6호
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    • pp.595-608
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    • 2021
  • Background and objective: For effective horticultural education, it is required to develop kits for practice. In this study, we developed a horticultural kit for germination that introduces storytelling, characterization, and assembly, and investigated the educational effects of using it to study its educational differentiation and usefulness. Methods: First, we developed a seed germination kit for learning. It consists of three components: the kit main frame for hydroponics, the activity sheet for storytelling, and the instruction manual. It is made into an acrylic assembly in consideration of the ease of delivery for contactless learning. Second, we developed two programs, each using a germination kit for horticultural education (experimental group) and a general germination kit program (control group). At this time, the class consists of pre-questionnaire, the 1st and 2nd classes, observation log writing (total six times for two weeks), the 3rd class, and post-questionnaire. The experimental group and control group were separately executed. Third, the application results were compared and analyzed before and after class. As a quantification questionnaire, the effects before and after class were compared through t-test with a questionnaire on program satisfaction and attitude change toward plants. The quantitative results were analyzed through observation activity sheets and effects before and after the class were analyzed through t-test with quantification and language coding. Results: The effect of horticulture education using the developed kit was estimated by a quantification questionnaire. Both the program satisfaction and the change in attitude toward plants showed high in the experimental group, and this difference was statistically significant (p < .05). As a result of analyzing the observation paper with a qualitative questionnaire, various cognitive and affective expressions appeared in the experimental group as well as the contents of the observation paper. The number of syllables and various qualitative expressions appeared, and these differences were also statistically significant (p < .05). Conclusion: Compared to general germination activities, it was found that the germination activities using storytelling-based horticultural kits would be effective for contactless classes in the post-COVID-19 era and in increasing the educational effect of horticultural activities.

FPCB 열압착 프레스 개발에 관한 연구 (A Study on the Development of FPCB Lamination Press)

  • 안재우;이창헌;변재혁;이종형
    • 한국산업융합학회 논문집
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    • 제11권4호
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    • pp.171-175
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    • 2008
  • The bigger size of the wafer to the meet the need of higher productivity and the smaller digital products for convene are examples of the main trends of the market among others. The higher integrity of chips is also an important task in semiconductor industry. In this thesis the COF(chop on film) technology has reviewed to investigate the best combinations of required functions and to develop a new system to save the installation area and to adapt to the variation of layout with flexibility. The new system shows the better and provides the improved safety with smaller size.

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부이기반 자율형 수상로봇키트 개발 (Development of Buoy-based Autonomous Surface Robot-kit)

  • 김현식
    • 한국해양공학회지
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    • 제29권3호
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    • pp.249-254
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    • 2015
  • Buoys are widely used in marine areas because they can mark positions and simultaneously acquire and exchange underwater, surface, and airborne information. Recently, the need for controlling and optimizing a buoy's position and attitude has been raised to achieve successful communication in a heterogeneous collaborative network composed of an underwater robot, a surface robot, and an airborne robot. A buoy in the form of a marine robot would be ideal to address this issue, as it can serve as a moving node of the communication network. Therefore, a buoy-based autonomous surface robot-kit with the abilities of sonar-based avoidance, dynamic position control, and static attitude control was developed and is discussed in this paper. The test and evaluation results of this kit show the possibility of real-world applications and the need for additional studies.

영상 인식 기반 신속 인플루엔자 자동 판독 기법 개발 (Development of Automated Rapid Influenza Diagnostic Test Method Based on Image Recognition)

  • 이지은;주윤하;이정찬
    • 대한의용생체공학회:의공학회지
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    • 제40권3호
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    • pp.97-104
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    • 2019
  • To examine different types of influenza diagnostic test kits automatically, automated rapid influenza diagnostic test method based on image recognition is proposed in this paper. First, the proposed methods classify a variety of the rapid influenza diagnostic test kit based on support vector machine that analyzes the kits' feature point. Then, to improve the accuracy of test, the proposed methods match the histogram of both the target image of influenza kit and the input image of influenza kit for minimizing the effect of environment factors, such as lighting and exposure variations. And, to minimize the effect from composition of the hand-helds devices, the proposed methods extract the feature point and match point-by-point between target image of influenza kit and input image of influenza kit. Experimental results of 124 experimental group show that the proposed methods significantly have effectiveness, which shows 90% accuracy in moderate antigen, for the preliminary examination of influenza, and provides the opportunity for taking action against influenza.

중학생들을 위한 얇은막 크로마토그래피 실험 키트의 개발 (Development of a Thin Layer Chromatography Experimental Kit for Middle School Students)

  • 류재정;박원규
    • 대한화학회지
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    • 제47권4호
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    • pp.401-411
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    • 2003
  • 제7차 교육과정의 가장 큰 특징은 수준별 교육과정의 시행이라고 할 수 있으며, 이에 따라 학생의 능력과 개인차에 따른 다양한 학습 과제의 개발이 필요하다. 제6차 교육과정의 중학교 과정에서 다루었던 크로마토그래피의 이론과 실험의 오류를 바로잡기위하여 중학생들이 크로마토그래피의 원리를 보다 정확하게 이해할 수 있는 새로운 실험 키트가 필요하다. 본 연구에서는 시중에 판매되고 있는 TLC판을 정지상으로 이용하고, 이동상으로는 상대적으로 인체에 해가 적은 혼합 유기용매를 사용하며, 시료 및 참고물질로는 시료의 전개 과정을 눈으로 쉽게 확인할 수 있는 산-염기 지시약들을 사용하여 새로운 크로마토그래피 실험 키트를 개발하였다. 또한, 이 실험 키트의 효과를 알아보기 위하여 중학생(일반 중학생 및 영재반 중학생)과 교사(과학 교사 및 비과학 교사)를 상대로 실험을 실시하여, 실험내용과 그 효과에 관한 설문을 조사하였다.

창의공학설계 및 실습 수업을 위한 아두이노 기반 교육용 캔위성 개발 연구 (A Study on Development of Educational CanSat based on Arduino for Creative Engineering Design and Practice Class)

  • 이영건;이상현;김종범;김송현;유승훈
    • 공학교육연구
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    • 제24권5호
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    • pp.38-45
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    • 2021
  • The CanSat was designed as an educational satellite simulation program that implements the overall system of the satellite such as the command processing unit, the communication unit, and the power unit in a structure of the size of a can. In particular, the training effect is very excellent because the trainee can learn a process similar to the actual satellite development process by designing, manufacturing, testing, and launching. Republic of Korea Air Force Academy has been using the CanSat production kit used by the domestic can satellite contest experience department for education, but since it was produced based on PCB, it was impossible to show creativity and operation was restricted even with small mistakes. In this paper, we analyze the existing CanSat kit and propose a new educational CanSat kit that can be used in creative engineering design and practice subjects that will be reorganized into a regular course from 2021, and a lesson plan. In conclusion, by using the proposed CanSat kit for lectures, it is possible to achieve educational purposes and effects, improve lecture satisfaction, and provide stable instruction.

A Study on Satisfaction with Makeup-kits and Makeup-kit Brand Development -Focused on Lifelong Education Centers of Colleges-

  • Kim, EunSil;Chung, Ju-Im
    • 패션비즈니스
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    • 제19권3호
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    • pp.31-42
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    • 2015
  • The purpose of this study is to develop a new makeup-kit brand for education, analyzing customer satisfaction of makeup-kits used in makeup lessons provided by lifelong education centers of colleges. The research method is as follows. In order to understand the current status of the kits used in makeup lessons offered by lifelong education centers of colleges in South Korea, the study conducted a survey from October 10 of 2014 to October 31 via fax, telephone and e-mail targeting these 98 colleges that were open makeup-courses available in October 2014. As for an analysis technique, the study used a frequency analysis. In order to develop a makeup-kit brand, the study investigated these makeup-kits as well and presented a BI draft and a makeup-kit design that the study had created for itself using Photoshop CS6 and Illustrator CS4. According to findings of the survey, the students were not satisfied with kits that had too many items, were high in price and had poor case design. In the light of that, the study conducted to develop a low to middle priced makeup-kit ranging from 110,000 KRW and 150,000 KRW with approximately 26 items. In terms of a case design, black was a dominant color which would make the case feel simple and modern. Dimensions of a small size makeup-kit container were 22cm*35cm*37cm with a sponge and rubber pad proposed as product ideas. The study also worked on a BI design.

Genome-Wide Transcriptional Response During the Development of Bleomycin-Induced Pulmonary Fibrosis in Sprague-Dawley Rats

  • Park, Han-Jin;Yang, Mi-Jin;Oh, Jung-Hwa;Yang, Young-Su;Kwon, Myung-Sang;Song, Chang-Woo;Yoon, Seok-Joo
    • Toxicological Research
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    • 제26권2호
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    • pp.137-147
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    • 2010
  • Pulmonary fibrosis is a common consequence of many lung diseases and a leading cause of morbidity and mortality. The molecular mechanisms underlying the development of pulmonary fibrosis remain poorly understood. One model used successfully to study pulmonary fibrosis over the past few decades is the bleomycin-induced pulmonary fibrosis model. We aimed to identify the genes associated with fibrogenesis using an Affymetrix GeneChip system in a bleomycin-induced rat model for pulmonary fibrosis. To confirm fibrosis development, several analyses were performed, including cellular evaluations using bronchoalveolar lavage fluid, measurement of lactate dehydrogenase activity, and histopathological examinations. Common aspects of pulmonary fibrosis such as prolonged inflammation, immune cell infiltration, emergence of fibroblasts, and deposition of extracellular matrix and connective tissue elements were observed. Global gene expression analysis revealed significantly altered expression of genes ($\geq$ 1.5-fold, p < 0.05.) in a time-dependent manner during the development of pulmonary fibrosis. Our results are consistent with previous results of well-documented gene expression. Interestingly, the expression of triggering receptor expressed on myeloid cells 2 (Trem2), secreted phosphoprotein 1 (Spp1), and several proteases such as Tpsab1, Mcpt1, and Cma1 was considerably induced in the lung after bleomycin treatment, despite little evidence that they are involved in pulmonary fibrogenesis. These data will aid in our understanding of fibrogenic mechanisms and contribute to the identification of candidate biomarkers of fibrotic disease development.

열간가공된 γ-TiAl 합금의 미세조직 제어 및 기계적 특성 평가 (Microstructure Control and Tensile Property Measurements of Hot-deformed γ-TiAl alloy)

  • 박성현;김재권;김성웅;김승언;박노진;오명훈
    • 열처리공학회지
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    • 제32권6호
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    • pp.256-262
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    • 2019
  • The microstructural features and texture development by both hot rolling and hot forging in ${\gamma}-TiAl$ alloy were investigated. In addition, additional heat treatment after hot forging was conducted to recognize change of the microstructure and texture evolution. The obtained microstructural features through dynamic recrystallization after hot deformed ${\gamma}-TiAl$ were quite different because two kinds of formation process were occurred depending on deformation condition. However, analyzed texture tends to be random orientation due to intermediate annealing up to ${\alpha}+{\beta}$ region during the hot deformation process. After additional heat treatment, microstructure transformed into fully lamellar microstructure and randomly oriented texture was also observed due to the same reason as before. Tensile test at room temperature demonstrated that anisotropy of mechanical properties were not appeared and transgranular fracture was occurred between interface of ${\alpha}_2/{\gamma}$. As a result, it could be suggested that microstructural features influenced much more than texture development on mechanical properties at room temperature.