• The Korean Journal of Mycology
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• v.27 no.6 s.93
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• pp.424-429
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• 1999

This study was conducted to investigate the characteristics of polysaccharides isolated from the fruit body and cultured mycelia of Phellinus linteus IY001. All fractions were extracted by hot water, followed by ethanol precipitation (F-THE and M-HE) or ultrafiltration (M-HU) (F-TH, F-THE; fruit body, M-HE, M-HU; cultured mycelia). Among these fractions, F-TH fraction was obtained at the highest yields of 6.83% and yield of F-THE was at the level 2.79%. The carbohydrates of these fractions was found to be a heteroglucan composed of glucose, galactose, mannose, fructose, ribose and xylose by analysis of gas chromatography. The total carbohydrate contents of M-HE and M-HU fractions were 99.2%, and 86.0% respectively. The glucose content of M-HE, M-HU and F-THE ranged from 54 to 84.8% of the total monosaccharide. Amino acid pattern showed that all fractions contained a large amount of aspartic acid, glycine, glutamic acid, alanine. Serine and threonine were found to be involved in the linkage, O-linked type. These fractions, except F. TH, contained polysaccharides with the molecular weights of 12 kD and showed the characteristics of IR absorption for ${\beta}-glucosides$ at $890\;cm^{-1}$.

• Journal of Physiology & Pathology in Korean Medicine
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• v.16 no.4
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• pp.674-679
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• 2002

The toxicity and bioaccumulation of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) and polychlorinated biphenyls (PCBs) continues to be a focus of research in human and various species. The main human exposure is via the dietary route. This study was carried out to investigate the protective effect of Cornu Cervi Parvum extract on clinical parameters and hepatotoxicity in Sprague-Dawley rat (SD rat) accutely exposured to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). Male SD rats received single intraperitoneal (ip) injection of TCDD (40 ㎍/kg), and administered 10 or 20 mg/kg/day of the ethanol extract oral injection for 4 weeks from 1 week before TCDD treatment. The gain in body weight was less in group treated with TCDD than in CON group, while that of C/H+ TCDD group (Cornu Cervi Parvum extract 20 mg/kg/day) increased. The decrease in spleen and testis weight caused by TCDD was prevented by Cornu Cervi Parvum extract 20 mg/kg/day. The fluctuation in BUN content, WBC and platelet count by TCDD intoxication were significantly attenuated by the ethanol extract treatment (20 mg/kg/day for 4 weeks). Treatments of rats with the extract (10 or 20 mg/kg/day) were significantly reduced AST and ALT levels compared with TCDD-treated group. Moderate swelling of hepatocytes, hyperchromatism, acidophilic cytoplasm and cytoplasmic vacuolation were observed in TCDD-treated animals (TCDD group). The administration of EtOH extract 10 or 20 mg/kg along with TCDD significantly alleviated the liver histopathological alteration induced by TCDD. These results suggest that Cornu Cervi Parvum extract can be useful as a protective agent against TCDD, an endocrine disruptor.

• Journal of Society of Preventive Korean Medicine
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• v.4 no.2
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• pp.235-241
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• 2000

This study was carried out to evaluate cytotoxic effects of Poncirus trifoliata Raf. extract on lymphocytic leukemia tumor (L1210) cell lines. Disruptions in cell organelles were determined by 3-(4,5-dimethyl thiazol-2-yl)-2,5-diphenyl-2H-tetrazoliumbromide (MTT) assay The comparison of Ic50 Values of Poncirus trifoliata Raf. extract in L1210 cell lines showed that their susceptibility to these fractons decreased in the following order: adriamycin > Fr.4> Fr. 6> Fr. 5> Fr. 3> Fr. 1> Fr. 2 by the MTT assay. In order to develop an antumicrobial agent, Poncirus trifoliata Raf. was extracted wit ethanol, and then it was fractionated with several mobile phase. The antitumor activities of fractions of the ethanol soluble extract was investigated. The minimal inhibitory concentrations (MIC) of fractions of the ethanol soluble extract of Poncirus trifoliata Raf. against microorganisms were also examined. Antimicrobial activities of ampicillin and ketoconazole as references were compared to those of fractions of the ethanol soluble extract of Poncirus trifoliata Raf. The antimicrobial activities of all fractions from the extract had growth inhibition activities against gram-positive bacteria, gram-negative bacteria and fungi $(MIC\;>\;200{\mu}g/ml)$. These results suggest that fraction 4 of the ethanol soluble extract of Poncirus trifoliata Raf. possessed the most antitumorous agent.

• Journal of Society of Preventive Korean Medicine
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• v.4 no.2
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• pp.273-284
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• 2000

The objective of this study consists on clarifying what factors are influenced upon skin care behavior using fender's Health Promotion Model, widely used to anticipate practices of health care behavior for women with keen interest in skin care and then manifesting their correlations from July 1 to August 22, 1998, on 159 women interested in skin care. A subvariable included practice of skin care behavior and independent variables include general characteristics, health fitness, the level of agreement with the advantages of the perceived skin care behavior, the level of cognition of the necessity for skin care behavior The data collected processed with ANOVA and multiple regression analysis to clarify what factors among other independent variables have the most powerful effects upon skin care. The resultant findings were revealed, as follows : 1. In health care behavior among skin care behavior,'full ingestion of water' represented the highest value by 3.45 points, 2. The testees of this study had for the most part a higher level of cognition for the necessity for skin care behavior than for practice of skin care behavior. The more points they have for cognition of the necessity for skin care behavior, the more points they present for practice of skin care behavior. 3. From the perspective of the relationship between sociometic factors on the testees and their skin care behavior, the higher level married women were on (p<0.05) and the more points they have for economic status, the higher points were reflected . 4. The correlations between the testees' recognition or perception factors and points for practice of skin health state showed the higher points than the group who thought that they did not know their own skin health state (p<0.05). The higher the points of skin care fitness (p<0.0001), the higher the points of self-awareness (p<0.0001) 5. The factors that had the most powerful influence upon cognition and practice of women's skin care behavior were found, with statistical significance, to be adjustable factors such as age, smoking, drinking habit, economic status, etc. and points of cognition of the necessity for skin care behavior.

• Research in Plant Disease
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• v.19 no.4
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• pp.326-330
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• 2013

During the 2012 growing season, 154 leaf samples were collected from sweet cherry trees in Hwaseong, Pyeongtaek, Gyeongju, Kimcheon, Daegu, Yeongju and Eumseong and tested for the presence of Cherry green ring mottle virus (CGRMV). PCR products of the expected size (807 bp) were obtained from 6 samples. The PCR products were cloned and sequenced. The nucleotide sequences of the clones showed over 88% identities to published coat protein sequences of CGRMV isolates in the GenBank database. The sequences of CGRMV isolates, CGR-KO 1-6 shared 98.8 to 99.8% nucleotide and 99.6 to 100% amino acid similarities. Phylogenetic analysis indicated that the Korean CGRMV isolates belong to the group II of CGRMV coat protein genes. The CGRMV infected sweet cherry trees were also tested for Apple chlorotic leaf spot virus (ACLSV), Apple mosaic virus (ApMV), Cherry necrotic rusty mottle virus (CNRMV), Cherry mottle leaf virus (CMLV), Cherry rasp leaf virus (CRLV), Cherry leafroll virus (CLRV), Cherry virus A (CVA), Little cherry virus 1 (LChV1), Prune dwarf virus (PDV) and Prunus necrotic ringspot virus (PNRSV) by RT-PCR. All of the tested trees were also infected with ACLSV.

• Korean Journal of Soil Science and Fertilizer
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• v.42 no.3
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• pp.145-151
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• 2009

This study was conducted to develop rational soil management and enhance the productivity of lands converted from paddy soils. Specifically, the changes in the soil physical properties brought about by the change in land usage from paddy soil were evaluated. This was carried out from 1999 to 2001 at 50 site in large-scale converted paddy fields of Kimcheon, Youngcheon, Gyeongsan and Milyang in the Youngnam region, categorized according to soil texture and drainage class. The ridge height of converted paddy soils was higher in coarse-textured and poorly-drained soils than in fine-textured and well-drained soils. The gray color of the surface soil was of lesser degree in converted soils than paddy soils and more notable in welldrained soils. The porosity ratio and the formation of aggregate structure were higher, and the appearance of soil mottling was deeper in converted paddy fields than in paddy soils. The glaying layer "g" of surface soil degraded with time. The porosity and amount of water stable aggregate was found to increase with time after conversion. The penetration resistance of the converted paddy soil was lower and deeper with time after conversion. The soil aeration of the converted paddy soil was lower in sandy loam than in loamy soil. Furthermore, soil aeration was influenced by ridge height and drainage class in poorly-drained soils.

• Journal of dental hygiene science
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• v.3 no.1
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• pp.39-44
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• 2003

This study was to investigate the antibacterial effect of Schizandrae fructus and Evodiae fructus extracts on S. mutans growth. The antibacterial effect of these extracts on S. mutans was good at Schizandrae fructus extract than the Evodiae fructus extract. The antibacterial activities of these extracts were measured 8.5 mm and 6.5 mm at 20 mg/ml concentration, respectively. The growth of S. mutans in control medium was the highest at 8 hrs, while the media of Schizandrae fructus and Evodiae fructus extracts added-medium(2 mg/ml) showed maximum growth at 16hrs. The pH values of the control medium was 5.08 at 8 hrs, but the media supplemented with Schizandrae fructus and Evodiae fructus extracts were 6.00, and 5.36 at 8 hrs, respectively. The amounts of total carbohydrate for the control media was 0.81 mg/ml at 8 hrs, but the media supplemented with Schizandrae and Evodiae fructus extracts were 1.32 mg/ml and 1.88 mg/ml at 8 hrs, respectively. In the change of culture medium proteins, the control culture broth and cultures supplemented with Schizandrae fructus and Evodiae fructus extracts were 8.39 mg/ml, 8.59 mg/ml and 9.97 mg/ml at 8 hrs, respectively. The S. mutans polysaccharide contents of the control medium and the media supplemented with Schizandrae and Evodiae fructus extracts were 300 mg/100 ml, 240 mg/100 ml and 280 mg/100 ml at 8 hrs, respectively.

• The Journal of Korean Academic Society of Nursing Education
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• v.5 no.2
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• pp.359-375
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• 1999

This is a survey in order to provide basic information about menstrual pain management and dysmenorrhea management. Menstrual pain means low abdominal cramps during menstruation and dysmenorrhea means low abdominal pains, and other symtoms as lumbago, fatigue, G-I symptoms, etc. The purposes of this study were (1) to measure the menstrual pain and dysmenorrhea, (2) to determine factors influenced to them, and (3) to know self-management method for them of college students. The subject of this study consisted of 312 college students Data were collected by questionnaire which was to measure the menstrual pain(Visual Analogue Scale(VAS), dysmenorrhea (dysmenorrhea scale) and general informations from May to June, 1999. The results of this study were as follows ; (1) Mean age for subjects was 19.4 yrs, their height was 161.4Cm, weight, 50.9Kg, menarcheal age, 13.5yrs, menstural period, 30.1 days, and menstrual duration was 5.6 days. Most respondents replied that their menstrual bleeding was moderate (72.1%) and occured regularly(55.4%). (2) Mean menstrual pain was 5.20. Menstrual pain didn't be occured 17.4 % of respondents, periodically 46.0%, and intermittently 36.6%, most commonly beginning the first day of menstruation (3) Chief management method was endurance (40.4%), use of analgesics (29.5%) and bed rest(16.5%). (4) Factors influenced to menstrual pain were analgesics use, heavy bleeding, time of menstrual pain, life change during menstruation. (5) Most symptoms of dysmenorrhea were low abdominal pain(22.2%), lumbago (15.9%), fatigue(9.0%). (6) Factors influenced to dysmenorrhea were anlgesics use and menstrual pain. (7) By Pearson's correlation analysis, there was a significant correlation between menstrual pain and menarcheal age, menstrual period. menstrual duration. But there was no correlation between menstrual pain and height, weight (8) By Pearson's correlation analysis, there was a significant correlation between dysmenorrhea and menstrual period But there was no correlation between menstrual pain and menarcheal age, menstrual duration, height, weight The results demonstrate the importance of development and clinical trial of nursing intervention on menstrual pain and dysmenorrhea.

• Research in Plant Disease
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• v.12 no.3
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• pp.205-212
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• 2006

Agrobacterium vitis is a causal agent of crown-gall disease on grapevine. Bacterial strains were isolated from crown galls of different grapevine varieties in grapevine farms of Kyungbuk(Kimcheon), Chungbuk(Okcheon), Chungnam(Daejon, Choenan) and Kyeonggi(Suwon, Ansung) areas in Korea from 2002 to 2005. Primer sets, Phe A and VirA, which were derived from pectate lysase gene and virA gene of Ti-plasmid in A. vitis were used to detect A. vitis strains from crown galls. PheA and VirA primers amplified DNA fragments of 0.25 kb and 0.5 kb from fifty-one bacterialstrains. They formed crown galls on grapevine variety, Kyoho, or carrot disks with variable pathgenecity It was confirmed that the biochemical characteristics of 10 bacterial strains that was strong pathogene city on grapevine were mostly in agreement with type culture strains of A. vitis, showing growth in the presence of 2% NaCl, non-production of acid from melezitose and negative response in production of 3-Ketolactose.

• Journal of the Korean Society of Food Science and Nutrition
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• v.29 no.4
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• pp.737-742
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• 2000

As functionality investigation of a soybean fermentation food, a angiotensin converting enzyme inhibitory peptide was separated during natto fermentation by Bacillus natto and inhibitory effect was investigated. After incubation at each 2$0^{\circ}C$, 3$0^{\circ}C$, 4$0^{\circ}C$, 5$0^{\circ}C$, 6$0^{\circ}C$ for the 0~72 hr, protein content, protease activity and angiotensin converting enzyme inhibition were determined. The protein content and protease activity were increased and reached maximum at 60 hr fermentation with 4$0^{\circ}C$ and decreased after the 60 hr fermentation during natto fermentation. The optimum condition for angiotensin converting enzyme inhibitors was appeared at fermentation for 60 hr at 4$0^{\circ}C$. Crude extract of natto was partially purified by Amicon membrane YM-3 and Sephadex G-10, G-25 gel filtration, stepwise. The inhibitory rate was increased in a concentration dependent manner, espcially the most potent activity about 74.74% at 1.0 mg peptide content. The most prominent amino acid of the peptide from natto was alanine, followed by phenylalnine, histidine.