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http://dx.doi.org/10.5423/RPD.2006.12.3.205

PCR based Rapid Isolation of Agrobacterium vitis Strains in Korea and Their Pathogenical and Biochemical Characteristics  

Kim, Jong-Kun (Graduate School of Biotechnology and Information Technology, Hankyong National University)
Lim, Sun-Hwa (Graduate School of Biotechnology and Information Technology, Hankyong National University)
Lee, Dae-Sung (Graduate School of Biotechnology and Information Technology, Hankyong National University)
Choi, Jae-Eul (College of Agriculture, Chungnam National University)
Yun, Hae-Keun (National Horticultural Research Institute, RDA)
Park, Sang-Hun (Agriculture Technology Center)
Kang, Sung-Su (Agriculture Technology Center)
Kang, Hee-Wan (Graduate School of Biotechnology and Information Technology, Hankyong National University)
Publication Information
Research in Plant Disease / v.12, no.3, 2006 , pp. 205-212 More about this Journal
Abstract
Agrobacterium vitis is a causal agent of crown-gall disease on grapevine. Bacterial strains were isolated from crown galls of different grapevine varieties in grapevine farms of Kyungbuk(Kimcheon), Chungbuk(Okcheon), Chungnam(Daejon, Choenan) and Kyeonggi(Suwon, Ansung) areas in Korea from 2002 to 2005. Primer sets, Phe A and VirA, which were derived from pectate lysase gene and virA gene of Ti-plasmid in A. vitis were used to detect A. vitis strains from crown galls. PheA and VirA primers amplified DNA fragments of 0.25 kb and 0.5 kb from fifty-one bacterialstrains. They formed crown galls on grapevine variety, Kyoho, or carrot disks with variable pathgenecity It was confirmed that the biochemical characteristics of 10 bacterial strains that was strong pathogene city on grapevine were mostly in agreement with type culture strains of A. vitis, showing growth in the presence of 2% NaCl, non-production of acid from melezitose and negative response in production of 3-Ketolactose.
Keywords
Agrobacterium vitis; Biochemical characteristics; Crown gall; Grapevine; Pathogenicity; Specific PCR detection;
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