• Genomics & Informatics
• /
• 제7권1호
• /
• pp.38-40
• /
• 2009

The EST Knowledge Integrated System, EKIS (http://ekis.kribb.re.kr), was established as a part of Korea's Ministry of Education, Science and Technology initiative for genome sequencing and application research of the biological model organisms (GEAR) project. The goals of the EKIS are to collect EST information from GEAR projects and make an integrated database to provide transcriptomic and metabolomic information for biological scientists. The EKIS constitutes five independent categories and several retrieval systems in each category for incorporating massive EST data from high-throughput sequencing of 65 different species. Through the EKIS database, scientists can freely access information including BLAST functional annotation as well as Genechip and pathway information for KEGG. By integrating complex data into a framework of existing EST knowledge information, the EKIS provides new insights into specialized metabolic pathway information for an applied industrial material.

• BMB Reports
• /
• 제40권1호
• /
• pp.95-99
• /
• 2007

Bovine coding region single nucleotide polymorphisms located proximal to quantitative trait loci were identified to facilitate bovine QTL fine mapping research. A total of 692,763 bovine SNPs was extracted from 39,432 UniGene clusters, and 53,446 candidate SNPs were found to be a depth >3. In order to validate the in silico SNPs experimentally, 186 animals representing 14 breeds and 100 mixed breeds were analyzed. Genotyping of 40 randomly selected candidate SNPs revealed that 43% of these SNPs ranged in frequency from 0.009 to 0.498. To identify non-synonymous SNPs and to correct for possible frameshift errors in the ESTs at the predicted SNP positions, we designed a program that determines coding regions by protein-sequence referencing, and identified 17,735 nsSNPs. The SNPs and bovine quantitative traits loci informations were integrated into a bovine SNP data: BcSNPdb (http://snugenome.snu.ac.kr/BtcSNP/). Currently there are 43 different kinds of quantitative traits available. Thus, these SNPs would serve as valuable resources for exploiting genomic variation that influence economically and agriculturally important traits in cows.

• 약학회지
• /
• 제56권5호
• /
• pp.280-287
• /
• 2012

A high-performance liquid chromatography (HPLC) combined with ultraviolet (UV) method for the simultaneous determination of ${\alpha}$-cyperone and nootkatone was developed for the quality control of Cyperus rotundus Linne. The separation was performed on a KR100-$5C_{18}$ ($4.6{\times}250mm$) column, and an elution gradient composed of methanol and water with a flow-rate of 1.0 ml/min. Detection wavelength was set at 254 nm. The optimum extraction for the detection of the ${\alpha}$-cyperone and nookatone was achieved by ultrasonic with methanol for an hour. Two marker compounds ${\alpha}$-cyperone and nootkatone in Cyperi Rhizoma showed good linearity ($R^2$ >0.999) in the concentration range of $12.5{\mu}g/ml$ to $200{\mu}g/ml$. The developed method provided satisfactory precision and accuracy with overall intra-day and inter-day variations of 0.04~1.23% and 0.08~0.68%, respectively, and the overall recoveries of 97.45~105.58% for the two compounds analyzed. Additionally, a difference was observed in the cluster analysis and principal component analysis between Cyperi Rhizoma in Korea and China. The result demonstrated that the principal component analysis is useful to distinguish between Cyperi Rhizoma in Korea and China.

• Journal of the Optical Society of Korea
• /
• 제13권1호
• /
• pp.65-74
• /
• 2009

Device quality indium tin oxide (ITO) films are deposited on glass substrates and ultra-thin diamond-like carbon films are deposited as a buffer layer on ITO by a pulsed Nd:YAG laser at 355 nm and 532 nm wavelength. ITO films deposited at room temperature are largely amorphous although their optical transmittances in the visible range are > 90%. The resistivity of their amorphous ITO films is too high to enable an efficient organic light-emitting device (OLED), in contrast to that deposited by a KrF laser. Substrate heating at $200^{\circ}C$ with laser wavelength of 355 nm, the ITO film resistivity decreases by almost an order of magnitude to $2{\times}10^{-4}\;{\Omega}\;cm$ while its optical transmittance is maintained at > 90%. The thermally induced crystallization of ITO has a preferred <111> directional orientation texture which largely accounts for the lowering of film resistivity. The background gas and deposition distance, that between the ITO target and the glass substrate, influence the thin-film microstructures. The optical and electrical properties are compared to published results using other nanosecond lasers and other fluence, as well as the use of ultra fast lasers. Molecularly doped, single-layer OLEDs of ITO/(PVK+TPD+$Alq_3$)/Al which are fabricated using pulsed-laser deposited ITO samples are compared to those fabricated using the commercial ITO. Effects such as surface texture and roughness of ITO and the insertion of DLC as a buffer layer into ITO/DLC/(PVK+TPD+$Alq_3$)/Al devices are investigated. The effects of DLC-on-ITO on OLED improvement such as better turn-on voltage and brightness are explained by a possible reduction of energy barrier to the hole injection from ITO into the light-emitting layer.

• JSTS:Journal of Semiconductor Technology and Science
• /
• 제10권1호
• /
• pp.66-77
• /
• 2010

Improvement in breakdown voltage ($BV_{ds}$) and speed of the device are the key issues among the researchers for enhancing the performance of HEMT. Increased speed of the device aspires for shortened gate length ($L_g$), but due to lithographic limitation, shortening $L_g$ below sub-micrometer requires the inclusion of various metal-insulator geometries like T-gate onto the conventional architecture. It has been observed that the speed of the device can be enhanced by minimizing the effect of upper gate electrode on device characteristics, whereas increase in the $BV_{ds}$ of the device can be achieved by considering the finite effect of the upper gate electrode. Further, improvement in $BV_{ds}$ can be obtained by applying field plates, especially at the drain side. The important parameters affecting $BV_{ds}$ and cut-off frequency ($f_T$) of the device are the length, thickness, position and shape of metal-insulator geometry. In this context, intensive simulation work with analytical analysis has been carried out to study the effect of variation in length, thickness and position of the insulator under the gate for various metal-insulator gate geometries like T-gate, $\Gamma$-gate, Step-gate etc., to anticipate superior device performance in conventional HEMT structure.

• 한국산업정보학회논문지
• /
• 제18권1호
• /
• pp.37-45
• /
• 2013

뇌졸중 환자들은 운동장애, 감각장애, 인지장애 등 다양한 후유증을 경험하게 되며 이를 극복하기 위하여 많은 재활훈련을 받게 된다. 이러한 재활훈련은 병원에서만 이루어 질 때 보다 일상 속에서 지속적으로 수행될 때 그 효과가 극대화 된다. 이를 위해서 다양한 가정용 훈련프로그램이 보급되었지만 이를 수행함에 있어서 적절한 피드백에 제공되어 지는 프로그램 부족한 실정이다. 따라서 본 연구에서 3차원 깊이 측정이 가능한 키넥트 센서를 이용하여 사용자에게 필요한 피드백을 제공 받을 수 있는 가정용 운동프로그램을 개발하고자 한다. 본 연구에서 제안된 프로그램은 사용자가 원할 때만 수행지식(knowledge of performance; KP) 피드백과 결과지식(knowledge of result; KR) 피드백을 선택적으로 제공하며 이러한 사용자 주도적인 선택적 피드백은 사용자의 운동효과를 극대화 시킬 것이다.

• 한국임상약학회지
• /
• 제23권3호
• /
• pp.248-255
• /
• 2013

Objective: This paper was aimed to investigate the current labelling trend of tar color additives in infant's drugs used in Korea and other countries. Method: The current labelling system of the tar color additives in infant's drugs was investigated using inserted papers for drug information of infant's drugs as well as three drug information internet sites popular in Korea including internet sites of foreign countries of USA, UK, Japan, and China for infant's drug information. Results: Among 325 drugs registered as syrup form for infant's drugs (sources, http://www.health.kr), 4 drugs indicated tar color related information on the internet and only 2 drugs indicated tar color additives' name. And from the investigated results of 76 inserted papers for infant's drug information, among 31 drugs containing tar dyes, in only 19 drugs the tar color additives' names were notated. Among 6 drugs which do not contain tar dyes, only 1 drug was notated as 'Dye-Free'. Conclusion: For advanced labelling system in Korea, we proposed the below guidelines. [1] The name of tar color additives used in infant's drugs should be notated even in drug information on internet sites. [2] All drug ingredients used in infant's drugs need to be separately notated as active and inactive species on both internet sites and inserted papers. [3] 'Dye-Free' notation should be mandatory for infant's drugs which do not contain tar color additives in drug information on the internet sites as well as inserted papers.

• Journal of Microbiology and Biotechnology
• /
• 제24권3호
• /
• pp.337-345
• /
• 2014

Pichia pastoris is one of the most widely used expression systems for the secretory expression of recombinant proteins. The secretory expression in P. pastoris usually makes use of the prepro $MAT{\alpha}$ sequence from Saccharomyces cerevisiae, which has a dibasic amino acid cleavage site at the end of the signal sequence. This is efficiently processed by Kex2 protease, resulting in the secretion of high levels of proteins to the medium. However, the proteins that are having the internal accessible dibasic amino acids such as KR and RR in the coding region cannot be expressed using this signal sequence, as the protein will be fragmented. We have identified a new signal sequence of 18 amino acids from a P. pastoris protein that can secrete proteins to the medium efficiently. The PMT1-gene-inactivated P. pastoris strain secretes a ~30 kDa protein into the extracellular medium. We have identified this protein by determining its N-terminal amino acid sequence. The protein secreted has four DDDK concatameric internal repeats. This protein was not secreted in the wild-type P. pastoris under normal culture conditions. We show that the 18-amino-acid signal peptide at the N-terminal of this protein is useful for secretion of heterologous proteins in Pichia.

• 한국항공우주학회지
• /
• 제31권1호
• /
• pp.97-103
• /
• 2003

연소기 내부에서 연소효율의 증대목적으로 사용되어지고 있는 후향계단후류의 측면제트 분사가 있는 초음속 난류유동장에 대한 수치적 연구를 수행하였다. 본 연구에서는 2차원 Navier_Stokes 방정식과 k-$\varepsilon$ 난류모델식을 고해상도 풍상차분법인 TVD 기법을 사용하여 비정상 유동장에 대한 수치계산을 수행하였다. 유동특성은 제트분사에 의한 마하면이 장애물 역할을 하여 후향계단과 마하면사이에 공동유동(cavity flow)과 비슷한 유동현상이 나타난다. 따라서, 공동 유동에서 발생하는 유동의 진동특성이 나타날 것을 예측할 수 있다. 수치계산 결과에서도 모맨텀플럭스와 분사위치에 따라, 공동유동에서 발생하는 진동특성을 갖는 비정상 유동 특성이 나타나는 것을 볼 수 있었다.

• 한국농공학회논문집
• /
• 제53권1호
• /
• pp.71-81
• /
• 2011

Due to increased human activities and intensive rainfall events in a watershed, soil erosion and sediment transport have been hot issues in many areas of the world. To evaluate soil erosion problems spatially and temporarily, many computer models have been developed and evaluated over the years. However, it would not be reasonable to apply the model to a watershed if topography and environment are different to some degrees. Also, source codes of these models are not always public for modification. The ArcGIS model builder provides ease-of-use interface to develop model by linking several processes and input/output data together. In addition, it would be much easier to modify/enhance the model developed by others. Thus, simple model was developed to decide soil erosion hot spot areas using ArcGIS model builder tool in this study. This tool was applied to a watershed to evaluate model performance. It was found that sediment yield was estimated to be 13.7 ton/ha/yr at the most severe soil erosion hot spot area in the study watershed. As shown in this study, the ArcGIS model builder is an efficient tool to develop simple models without professional programming abilities. The model, developed in this study, is available at http://www.EnvSys.co.kr/~sateec/toolbox for free download. This tool can be easily modified for further enhancement with simple operations within ArcGIS model builder interface. Although very simple soil erosion and sediment yield were developed using model builder and applied to study watershed for soil erosion hot spot area in this study. The approaches shown in this study provides insights for model development and code sharing for the researchers in the related areas.