• BMB Reports
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• v.46 no.11
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• pp.561-566
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• 2013

We examined the ways in which fenobam could promote not only the transduction of PEP-1-FK506BP into cells and tissues but also the neuroprotective effect of PEP-1-FK506BP against ischemic damage. Fenobam strongly enhanced the protective effect of PEP-1-FK506BP against $H_2O_2$-induced toxicity and DNA fragmentation in C6 cells. In addition, combinational treatment of fenobam with PEP-1-FK506BP significantly inhibited the activation of Akt and MAPK induced by $H_2O_2$, compared to treatment with PEP-1-FK506BP alone. Interestingly, our results showed that fenobam significantly increased the transduction of PEP-1-FK506BP into both C6 cells and the hippocampus of gerbil brains. Subsequently, a transient ischemic gerbil model study demonstrated that fenobam pretreatment led to the increased neuroprotection of PEP-1-FK506BP in the CA1 region of the hippocampus. Therefore, these results suggest that fenobam can be a useful agent to enhance the transduction of therapeutic PEP-1-fusion proteins into cells and tissues, thereby promoting their neuroprotective effects.

• The Journal of Korean Medical History
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• v.26 no.2
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• pp.175-185
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• 2013

Purpose : The current Medical Law and the Pharmaceutical Affairs Act, which are incapable of utilizing the research results and the advanced academic, clinical, and pharmaceutical system of the present-day Korean (Oriental) medicine, have limitations and create a paradox by provoking social conflict among the professionals in the field. The aim of this study was to find out the legal and systematic problems that contributed to a complicated conflict amongst Korean (Oriental) medicine doctors, doctors, pharmacists, and Korean (Oriental) pharmacists regarding the classification of their functions. Methods : We reviewed the history and characteristics of the legislation regarding the duties of Korean (Oriental) medicine doctors and Korean (Oriental) pharmacists as well as the relevant and important public health policies since the enactment of the National Medical Services Law in 1951. We focused on the laws and regulations that are made in the process of the separating functions of physicians and pharmacists and the dispute between the Korean (Oriental) medicine doctors and the Korean (Oriental) pharmacists in the 1990s and 2000s. Results : The legislations and amendments of the medical and pharmaceutical laws and regulations that reflect the modern academic, clinical, and pharmaceutical system of the Korean (Oriental) medicine and the research results could be summarized as follows: 1) A partial amendment of the Medical Law in 1987, which added the provision of "Oriental health guidance" as one of the duties of Korean (Oriental) medicine doctors, assured a place for Korean (Oriental) medicine doctors in the field of public health. 2) A partial revision of Pharmaceutical Affairs Act in 1994 established a new system for Korean (Oriental) pharmacists, bringing about the creation of dualistic pharmaceutical system that complements the dualistic medical system. 3) The Promotion of the Research and Development of Wonder Drugs by Using Natural Substances Act was legislated in 2000 in order to stimulate research and development of Korean (Oriental) medicine and its industrialization. 4) Oriental Medicine Promotion Act in 2003 was enacted to lay foundation to specify and promote technology and industry that are related to Korean (Oriental) medicine. Discussions and conclusions : Although the dualistic medical and pharmaceutical system is set up by the Medical Law and Pharmaceutical Affairs Act, it is shown that the relevant regulations have been developed from a perspective of the western medicine.

• The Journal of Korean Medical History
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• v.26 no.2
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• pp.9-21
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• 2013

Purpose : The purpose of this study was to analyze the history and characteristics of laws and regulations of the medical and pharmaceutical system in Korea-focusing on the Korean (Oriental) medical and pharmaceutical system-from the modern period to the early days of the Republic. We reviewed how traditional notions and categories of Oriental medicine, which were regarded as experiential and conventional, became part of the current dualistic medical and pharmaceutical system, and examined problems and effects during the course of positioning. Methods : We classified the development of the medical and pharmaceutical laws and regulations chronologically, from the Korean Empire to the beginning of the Republic. The abolishment of the traditional medical system that was based on laws and regulations of the Joseon Dynasty, the implementation of dualistic medical system in the Korean Empire, the attempt to demolish Korean (Oriental) medicine under the Japanese colonial rule, and the process of developing a statute-based continental law system were thoroughly reviewed. Results : Although the dualistic medical system was specified in legislation via the enactment of the National Medical Services Law in 1951, we found that it was actually enacted in 1963, when the laws and systems regarding the educational institution of Korean (Oriental) medicine were stably established. Moreover, the dualistic pharmaceutical system was specified in legislation through the partial amendment of the Pharmaceutical Affairs Act in 1994, but we concluded that the actual enactment was rather in 2000, when the first Korean (Oriental) pharmacist was produced. Discussions and conclusions : An effort to establish a dualistic medical system of Korean (Oriental) medicine and Western medicine during the Korean Empire bore fruit a few decades later, after the Republic of Korea was founded. It means the basis for the legal system finally took shape in spite of the numerous attempts during the Japanese colonial era and the beginning of the Republic to abolish Korean (Oriental) medical and pharmaceutical system.

• Research in Plant Disease
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• v.17 no.2
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• pp.155-160
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• 2011

To develop the efficient screening methods for antifungal compound active to Chinese cabbage clubroot caused by Plasmodiophora brassicae, the control efficacy of three fungicides fluazinam, ethaboxam, and cyazofamid on the disease was tested under several conditions such as soil types, cultivars of Chinese cabbage, growth stages of the host, and inoculum concentrations. The in vivo antifungal activities of the fungicides on clubroot of two Chinese cabbage cultivars were hardly different. At 7- and 14-day-old seedlings, the fungicides were more effective to control of clubroot than at 21-day-old seedlings. In a commercial horticulture media soil (CNS), disease severity of untreated controls was higher and control activity of the fungicides was less than in a mixture of CNS and upland soil (1:1, v/v). Disease development of the seedlings inoculated with P. brassicae at $1.8{\times}10^7$ spores/pot to $1.1{\times}10^9$ spores/pot was almost same, but control efficacy of the fungicides was negatively correlated with inoculum dosages. To effectively select in vivo antifungal compound on Chinese cabbage clubroot, 14-day-old seedlings need to be inoculated with P. brassicae by drenching the spore suspension to give $1{\times}10^8$ spores/pot 1 day after chemical treatment. To develop clubroot, the inoculated plants are incubated in a growth chamber at $20^{\circ}C$ for 2 days, and then cultivated in a greenhouse ($20{\pm}5^{\circ}$) for four weeks.

• Journal of Physiology & Pathology in Korean Medicine
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• v.29 no.5
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• pp.378-385
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• 2015

Dizziness is one of the most common symptoms in clinical practice. The purpose of this study was to investigate the characteristics of patients with dizziness who visited Korean medicine hospital. This study analyzed 328 patients with dizziness who visited Dunsan Korean Medicine Hospital of Dae-Jeon University from Nov. 1st, 2012 to Oct. 31st, 2014. We gathered the data of the patients based on the medical charts. The patients were divided into 4 gorups ; central dizziness group, peripheral dizziness group, presyncope dizziness group, functional dizziness group, In the distribution according to sex, the number of female patients was higher than the male patients in all the types of dizziness except the presyncope group. The average age of the central group was higher than any other types. The peripheral and central group were most frequently admitted. The average hospitalization period were categorized as follows : central group 37.31, presyncope group 17, functional group 14.21, peripheral group 13.5 days. The following numbers of patients showed the distribution of each syndrome differentiation ; deficiency of qi and blood(氣血兩虛) 58.8%, plegm-dampness syndrome(濕痰中阻) 18.0%, hyperactivity of liver yang(肝陽上亢) 14.3%, deficiency of kidney essence(腎精不足) 8.8%. The distribution of herbal medicine for dizziness were categorized as follows ; Jaeumkeonbi-tang(Ziyinjianpi-tang, 滋陰健脾湯) 50.4%, Banhabaekchulcheonma-tang(Banxiabaishutianma-tang, 半夏白朮天麻湯) 10.2% and Bojungikgi-tang(Buzhongyiqi-tang, 補中益氣湯) 4.1%. The 86.0% of patients with dizziness has improved. The peripheral showed highest rate of improvement(100%). This report would serve as a reference data for further study about patients with dizziness in the clinical field of Korean medicine.

• Tuberculosis and Respiratory Diseases
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• v.81 no.3
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• pp.247-255
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• 2018

Background: Patients with acute respiratory failure secondary to tuberculous destroyed lung (TDL) have a poor prognosis. The aim of the present retrospective study was to develop a mortality prediction model for TDL patients who require mechanical ventilation. Methods: Data from consecutive TDL patients who had received mechanical ventilation at a single university-affiliated tertiary care hospital in Korea were reviewed. Binary logistic regression was used to identify factors predicting intensive care unit (ICU) mortality. A TDL on mechanical Ventilation (TDL-Vent) score was calculated by assigning points to variables according to ${\beta}$ coefficient values. Results: Data from 125 patients were reviewed. A total of 36 patients (29%) died during ICU admission. On the basis of multivariate analysis, the following factors were included in the TDL-Vent score: age ${\geq}65$ years, vasopressor use, and arterial partial pressure of oxygen/fraction of inspired oxygen ratio <180. In a second regression model, a modified score was then calculated by adding brain natriuretic peptide. For TDL-Vent scores 0 to 3, the 60-day mortality rates were 11%, 27%, 30%, and 77%, respectively (p<0.001). For modified TDL-Vent scores 0 to ${\geq}3$, the 60-day mortality rates were 0%, 21%, 33%, and 57%, respectively (p=0.001). For both the TDL-Vent score and the modified TDL-Vent score, the areas under the receiver operating characteristic curve were larger than that of other illness severity scores. Conclusion: The TDL-Vent model identifies TDL patients on mechanical ventilation with a high risk of mortality. Prospective validation studies in larger cohorts are now warranted.

• Journal of IKEEE
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• v.18 no.4
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• pp.485-494
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• 2014

Traffic Sign Recognition(TSR) is an important element in an Advanced Driver Assistance System(ADAS). However, many studies related to TSR approaches only in normal daytime environment because a sign's unique color doesn't appear in poor environment such as night time, snow, rain or fog. In this paper, we propose a new TSR algorithm based on machine learning for daytime as well as poor environment. In poor environment, traditional methods which use RGB color region doesn't show good performance. So we extracted sign characteristics using HoG extraction, and detected signs using a Support Vector Machine(SVM). The detected sign is recognized by a decision tree based on 25 reference points in a Normalized RGB system. The detection rate of the proposed system is 96.4% and the recognition rate is 94% when applied in poor environment. The testing was performed on an Intel i5 processor at 3.4 GHz using Full HD resolution images. As a result, the proposed algorithm shows that machine learning based detection and recognition methods can efficiently be used for TSR algorithm even in poor driving environment.

• BMB Reports
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• v.48 no.7
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• pp.395-400
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• 2015

Parkinson's disease (PD) is a neurodegenerative disability caused by a decrease of dopaminergic neurons in the substantia nigra (SN). Although the etiology of PD is not clear, oxidative stress is believed to lead to PD. Catalase is antioxidant enzyme which plays an active role in cells as a reactive oxygen species (ROS) scavenger. Thus, we investigated whether PEP-1-Catalase protects against 1-methyl-4-phenylpyridinium (MPP⁺) induced SH-SY5Y neuronal cell death and in a 1-methyl-4-phenyl-1,2,3,6-trtrahydropyridine (MPTP) induced PD animal model. PEP-1-Catalase transduced into SH-SY5Y cells significantly protecting them against MPP⁺-induced death by decreasing ROS and regulating cellular survival signals including Akt, Bax, Bcl-2, and p38. Immunohistochemical analysis showed that transduced PEP-1-Catalase markedly protected against neuronal cell death in the SN in the PD animal model. Our results indicate that PEP-1-Catalase may have potential as a therapeutic agent for PD and other oxidative stress related diseases. [BMB Reports 2015; 48(7): 395-400]

• Journal of Oriental Neuropsychiatry
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• v.7 no.1
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• pp.1-13
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• 1996

1. Out of 21 examples on a clinical base Alzheimer type dementia were 8 examples(38%), 11 vascular types(52%), 1 Alzheimer and vascular mixed type and rest 1 was secondary dementia type(NPH).2. Between the genders, there were 5 males and 3 females for Alzheimer types, 6 males and 5 females for the vascular types, 1 male for Alzheimer and vascular mixed type, and 1 female for secondary dementia type(NPH).3. For the degrees of Alzheimer type dementia there were 2 mild dementia, 4 moderate dementia, and 2 severe dementia. 4. Among the Alzheimer types 2 mild dementia were almost recovered back to normal in approximately 2 months, 2of 4 moderate dementia became significantly better in approximately 2 months and recovered to almost normal state in 3 to 5 months. The rest 2 have been under treatment for 3 months, but showed a little improvement. Out of 2 severe dementia examples, one showed a little improvement even if it has been under treatment over 2 years. The other example did not show any improvement, but dementia did not proceed any more.5. Among the 11 vascular examples, 7 recovered in 2 to 3 months, 1 in 5 months, 2 recovered in a year, and the treatment was stopped arbitrary for the last one.6 In the case of Alzheimer and vascular mixed type dementia, even the moderate dementia did not show any apparent result in 6 months.7. For NPHI (Normal Pressure Hydrocephalus), there was an improvement on dementia in 2 months after the treatment, but gait disturbance and urinary incontinence did not show any noticeable difference. As a conclusion, almost all the Alzheimer and vascular type patients recovered, but the treatment periods varied depending on the types of the dementia and the degree of seriousness, especially in the case of the severe Alzheimer patients, the treatment oniy suppression the progression of the dementia. The most important aspect from clinical point of view was even if almost all the patients were almost completely cired, they need to keep being cured for a long period of time. The method with respect to Four Constitutions is thought of the most desirable.

• Korean Journal of Medicinal Crop Science
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• v.10 no.1
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• pp.41-45
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• 2002

An efficient and reproducible procedure for the large scale propagation of Hovenia dulcis Thunb. is described. Shoot primodia emerging from the leaf surface was induced from MS medium supplemented with NAA. Stem cuttings were suitable explants for multiple shoot proliferation. They produced axillary shoots which branched repeatedly, yielding an average of 7 shoots per explants after 4 weeks in culture, when cultured on a woody plant medium (WPM) containing 0.1mg/l BA and 0.1mg/l NAA. Stem, leaf and root segments from axenic seedlings were used as explant source to induce somatic embryogenesis. A high frequency of somatic embryos were induced directly from leaf in MS medium with NAA, 2,4-D and in medium containing NAA, 2,4-D with BA. Somatic embryos were germinated in MS medium supplemented with 1mg/ l $GA_3$. Somatic embryos proliferated secondary somatic embryos rapidly after transfer to MS medium supplemented with 1mg/ l kinetin, 1mg/ l $GA_3$ and 2% dextrose.