Browse > Article
http://dx.doi.org/10.5483/BMBRep.2013.46.11.080

Fenobam promoted the neuroprotective effect of PEP-1-FK506BP following oxidative stress by increasing its transduction efficiency  

Ahn, Eun Hee (Department of Biomedical Science and Research Institute of Bioscience and Biotechnology, Hallym University)
Kim, Dae Won (Department of Biomedical Science and Research Institute of Bioscience and Biotechnology, Hallym University)
Shin, Min Jea (Department of Biomedical Science and Research Institute of Bioscience and Biotechnology, Hallym University)
Jo, Hyo Sang (Department of Biomedical Science and Research Institute of Bioscience and Biotechnology, Hallym University)
Eom, Seon Ae (Department of Biomedical Science and Research Institute of Bioscience and Biotechnology, Hallym University)
Kim, Duk-Soo (Department of Anatomy, College of Medicine, Soonchunhyang University)
Park, Eun Young (Department of Biological Sciences, Sookmyung Women's University)
Park, Jong Hoon (Department of Biological Sciences, Sookmyung Women's University)
Cho, Sung-Woo (Department of Biochemistry and Molecular Biology, University of Ulsan College of Medicine)
Park, Jinseu (Department of Biomedical Science and Research Institute of Bioscience and Biotechnology, Hallym University)
Eum, Won Sik (Department of Biomedical Science and Research Institute of Bioscience and Biotechnology, Hallym University)
Son, Ora (Department of Biomedical Science and Research Institute of Bioscience and Biotechnology, Hallym University)
Hwang, Hyun Sook (Department of Biomedical Science and Research Institute of Bioscience and Biotechnology, Hallym University)
Choi, Soo Young (Department of Biomedical Science and Research Institute of Bioscience and Biotechnology, Hallym University)
Publication Information
BMB Reports / v.46, no.11, 2013 , pp. 561-566 More about this Journal
Abstract
We examined the ways in which fenobam could promote not only the transduction of PEP-1-FK506BP into cells and tissues but also the neuroprotective effect of PEP-1-FK506BP against ischemic damage. Fenobam strongly enhanced the protective effect of PEP-1-FK506BP against $H_2O_2$-induced toxicity and DNA fragmentation in C6 cells. In addition, combinational treatment of fenobam with PEP-1-FK506BP significantly inhibited the activation of Akt and MAPK induced by $H_2O_2$, compared to treatment with PEP-1-FK506BP alone. Interestingly, our results showed that fenobam significantly increased the transduction of PEP-1-FK506BP into both C6 cells and the hippocampus of gerbil brains. Subsequently, a transient ischemic gerbil model study demonstrated that fenobam pretreatment led to the increased neuroprotection of PEP-1-FK506BP in the CA1 region of the hippocampus. Therefore, these results suggest that fenobam can be a useful agent to enhance the transduction of therapeutic PEP-1-fusion proteins into cells and tissues, thereby promoting their neuroprotective effects.
Keywords
Fenobam; Ischemic damage; Neuroprotection; PEP-1-FK506BP; Protein transduction domains;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
연도 인용수 순위
1 Kang, C. B., Hong, Y., Dhe-Paganon, S. and Yoon, H. S. (2008) FKBP family proteins: immunophilins with versatile biological functions. Neurosignals 16, 318-325.   DOI   ScienceOn
2 Kino, T., Hatanaka, H., Miyata, S., Inamura, N., Nishiyama, M., Yajima, T., Goto, T., Okuhara, M., Kohsaka, M., Aoki, H. and Ochiai, T. (1987) FK-506, a novel immunosuppressant isolated from a Streptomyces. II. Immunosuppressive effect of FK-506 in vitro. J. Antibiot. (Tokyo) 40, 1256-1265.   DOI
3 Harrar, Y., Bellini, C. and Faure, J. D. (2001) FKBPs: at the crossroads of folding and transduction. Trends Plant Sci. 6, 426-431.   DOI   ScienceOn
4 Brillantes, A. B., Ondrias, K., Scott, A., Kobrinsky, E., Ondriasova, E., Moschella, M. C., Jayaraman, T., Landers, M., Ehrlich, B. E. and Marks, A. R. (1994) Stabilization of calcium release channel (ryanodine receptor) function by FK506-binding protein. Cell 77, 513-523.   DOI   ScienceOn
5 Cameron, A. M., Nucifora, F. C., Fung, E. T., Livingston, D. J., Aldape, R. A., Ross, C. A. and Snyder, S. H. (1997) FKBP12 binds the inositol 1,4,5-trisphosphate receptor at leucine-proline (1400-1401) and anchors calcineurin to this FK506-like domain. J. Biol. Chem. 272, 27582-27588.   DOI   ScienceOn
6 Chen, Y. G., Liu, F. and Massague, J. (1997) Mechanism of TGFbeta receptor inhibition by FKBP12. EMBO J. 16, 3866-3876.   DOI   ScienceOn
7 Aghdasi, B., Ye, K., Resnick, A., Huang, A., Ha, H. C., Guo, X., Dawson, T. M., Dawson, V. L. and Snyder, S. H. (2001) FKBP12, the 12-kDa FK506-binding protein, is a physiologic regulator of the cell cycle. Proc. Natl. Acad. Sci. U. S. A. 98, 2425-2430.   DOI   ScienceOn
8 Shou, W., Aghdasi, B., Armstrong, D. L., Guo, Q., Bao, S., Charng, M. J., Mathews, L. M., Schneider, M. D., Hamilton, S. L. and Matzuk, M. M. (1998) Cardiac defects and altered ryanodine receptor function in mice lacking FKBP12. Nature 391, 489-492.   DOI   ScienceOn
9 Palucha, A. and Pilc, A. (2007) Metabotropic glutamate receptor ligands as possible anxiolytic and antidepressant drugs. Pharmacol. Ther. 115, 116-147.   DOI   ScienceOn
10 Porter, R. H., Jaeschke, G., Spooren, W., Ballard, T. M., Buttelmann, B., Kolczewski, S., Peters, J. U., Prinssen, E., Wichmann, J., Vieira, E., Muhlemann, A., Gatti, S., Mutel, V. and Malherbe, P. (2005) Fenobam: a clinically validated nonbenzodiazepine anxiolytic is a potent, selective, and noncompetitive mGlu5 receptor antagonist with inverse agonist activity. J. Pharmacol. Exp. Ther. 315, 711-721.   DOI   ScienceOn
11 Burke, R. E. (2007) Inhibition of mitogen-activated protein kinase and stimulation of Akt kinase signaling pathways: Two approaches with therapeutic potential in the treatment of neurodegenerative disease. Pharmacol. Ther. 114, 261-277.   DOI   ScienceOn
12 Hausenloy, D.J. and Yellon, D. M. (2004) New directions for protecting the heart against ischaemia-reperfusion injury: targeting the Reperfusion Injury Salvage Kinase (RISK)-pathway. Cardiovasc Res. 61, 448-460.   DOI   ScienceOn
13 Kim, S. Y., Sohn, E. J., Kim, D. W., Jeong, H. J., Kim, M. J., Kang, H. W., Shin, M. J., Ahn, E. H., Kwon, S. W., Kim, Y. N., Kwon, H. J., Kim, T. Y., Lee, K. S., Park, J., Eum, W. S. and Choi, S. Y. (2011) Transduced PEP-1-FK506BP ameliorates atopic dermatitis in NC/Nga mice. J. Invest. Dermatol. 131, 1477-1485.   DOI   ScienceOn
14 Ahn, E. H., Kim, D. W., Kim, D. S., Woo, S. J., Kim, H. R., Kim, J., Lim, S. S., Kang, T. C., Kim, D. J., Suk, K. T., Park, J., Luo, Q., Eum, W. S., Hwang, H. S. and Choi, S. Y. (2011) Levosulpiride, (S)-(-)-5-Aminosulfonyl-N-)(1-ethyl-2- pyrrolidinyl) methyl]-2-methoxybenzamide, enhances the transduction efficiency of PEP-1-ribosomal protein S3 in vitro and in vivo. BMB Rep. 44, 329-334.   과학기술학회마을   DOI   ScienceOn
15 Sohn, E. J., Kim, D. W., Kim, Y. N., Kim, S. M., Lim, S. S., Kang, T. C., Kwon, H. Y., Kim, D. S., Cho, S. W., Han, K. H., Park, J., Eum, W. S., Hwang, H. S. and Choi, S. Y. (2011) Effects of pergolide mesylate on transduction efficiency of PEP-1-catalase protein. Biochem. Biophys. Res. Commun. 406, 336-340.   DOI   ScienceOn
16 Koenitzer, J. R. and Freeman, B. A. (2010) Redox signaling in inflammation: interactions of endogenous electrophiles and mitochondria in cardiovascular disease. Ann. N Y Acad. Sci. 1203, 45-52.   DOI   ScienceOn
17 Yoon, J. H., An, S. H., Kyeong, I. G., Lee, M. S., Kwon, S. C. and Kang, J. H. (2011) Oxidative modification of ferritin induced by hydrogen peroxide. BMB Rep. 44, 165-169.   과학기술학회마을   DOI   ScienceOn
18 Zweier, J. L. and Talukder, M. A. (2006) The role of oxidants and free radicals in reperfusion injury. Cardiovasc. Res. 70, 181-190.   DOI   ScienceOn
19 Sofroniew, M. V. and Vinters, H. V. (2010) Astrocytes: biology and pathology. Acta. Neuropathol. 119, 7-35.   DOI   ScienceOn
20 van den Berg, A. and Dowdy, S. F. (2011) Protein transduction domain delivery of therapeutic macromolecules. Curr. Opin. Biotechnol. 22, 888-893.   DOI   ScienceOn
21 Kim, S. Y., Jeong, H. J., Kim, D. W., Kim, M. J., An, J. J., Sohn, E. J., Kang, H. W., Shin, M. J., Ahn, E. H., Kwon, S. W., Kim, D. S., Cho, S. W., Park, J., Eum, W. S. and Choi, S. Y. (2011) Transduced PEP-1-FK506BP inhibits the inflammatory response in the Raw 264.7 cell and mouse models. Immunobiology 216, 771-781.   DOI   ScienceOn
22 Anwyl, R. (1999) Metabotropic glutamate receptors: electrophysiological properties and role in plasticity. Brain Res. Rev. 29, 83-120.   DOI   ScienceOn
23 Niswender, C. M. and Conn, P. J. (2010) Metabotropic glutamate receptors: physiology, pharmacology, and disease. Annu. Rev. Pharmacol. Toxicol. 50, 295-322.   DOI   ScienceOn
24 Lindemann, L., Jaeschke, G., Michalon, A., Vieira, E., Honer, M., Spooren, W., Hartung, T., Kolczewski, S., Buttelmann, B., Flament, C., Diener, C., Fischer, C., Gatti, S., Prinssen, E. P., Parrott, N., Hoffmann, G. and Wettstein, J. G. (2011) CTEP: a novel, potent, long-acting, and orally bioavailable metabotropic glutamate receptor 5 inhibitor. J. Pharmacol. Exp. Ther. 339, 474-486.   DOI   ScienceOn
25 Veloz, M. F., Buijsen, R. A. M., Willemsen, R., Cupido, A., Bosman, L. W. J., Koekkoek, S. K. E., Potters, J. W., Oostra, B. A. and De Zeeuw, C. I. (2012) The effect of an mGluR5 inhibitor on procedural memory and avoidance discrimination impairments in Fmr1 KO mice. Genes Brain Behav. 11, 326-331.
26 Crock, L. W., Stemler, K. M., Song, D. G., Abbish, P., Voqt, S. K., Qiu, C. S., Lai, H. H., Mysorekar, I. U. and Gereau, R. W. (2012) Metabotropic glutamate receptor 5 (mGluR5) regulates bladder nociception. Mol. Pain. 8, 20.   DOI   ScienceOn
27 Hwang, I. K., Yoo, K. Y., Kim, D. W., Lee, C. H., Choi, J. H., Kwon, Y. G., Kim, Y. M., Choi, S. Y. and Won, M. H. (2010) Changes in the expression of mitochondrial peroxiredoxin and thioredoxin in neurons and glia and their protective effects in experimental cerebral ischemic damage. Free Radic. Biol. Med. 48, 1242-1251.   DOI   ScienceOn