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Somatic embryogenesis and plant regeneration of Hovenia dulcis Thunb  

Eom, Seung-Hee (College of Agriculture & Life Science, Kangwon National University)
Shin, Dong-Yong (College of Agriculture & Life Science, Kangwon National University)
Lee, Hyeon-Yong (College of Agriculture & Life Science, Kangwon National University)
Kim, Myong-Jo (College of Agriculture & Life Science, Kangwon National University)
Kim, Jong-Dai (College of Agriculture & Life Science, Kangwon National University)
Choi, Won-Cheol (Kwanghyeowon Medical Foundation Cancer Center)
Heo, Kwon (College of Agriculture & Life Science, Kangwon National University)
Yu, Chang-Yeon (College of Agriculture & Life Science, Kangwon National University)
Publication Information
Korean Journal of Medicinal Crop Science / v.10, no.1, 2002 , pp. 41-45 More about this Journal
Abstract
An efficient and reproducible procedure for the large scale propagation of Hovenia dulcis Thunb. is described. Shoot primodia emerging from the leaf surface was induced from MS medium supplemented with NAA. Stem cuttings were suitable explants for multiple shoot proliferation. They produced axillary shoots which branched repeatedly, yielding an average of 7 shoots per explants after 4 weeks in culture, when cultured on a woody plant medium (WPM) containing 0.1mg/l BA and 0.1mg/l NAA. Stem, leaf and root segments from axenic seedlings were used as explant source to induce somatic embryogenesis. A high frequency of somatic embryos were induced directly from leaf in MS medium with NAA, 2,4-D and in medium containing NAA, 2,4-D with BA. Somatic embryos were germinated in MS medium supplemented with 1mg/ l $GA_3$. Somatic embryos proliferated secondary somatic embryos rapidly after transfer to MS medium supplemented with 1mg/ l kinetin, 1mg/ l $GA_3$ and 2% dextrose.
Keywords
Somatic embryogenesis; Hovenia dulcis;
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